Streptococcus satellite phage Javan761
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A169|A0A4D6A169_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan761 OX=2558857 GN=JavanS761_0023 PE=4 SV=1
MM1 pKa = 7.22 TLDD4 pKa = 4.01 LGKK7 pKa = 8.39 MTQAEE12 pKa = 4.45 FDD14 pKa = 3.72 EE15 pKa = 4.66 VMADD19 pKa = 3.21 IKK21 pKa = 11.13 ARR23 pKa = 11.84 NPNLLQLITDD33 pKa = 4.52 FLDD36 pKa = 3.62 RR37 pKa = 11.84 KK38 pKa = 8.24 VTPEE42 pKa = 4.05 EE43 pKa = 3.86 VDD45 pKa = 3.54 DD46 pKa = 4.24
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.494
IPC2_protein 4.113
IPC_protein 3.986
Toseland 3.783
ProMoST 4.05
Dawson 3.986
Bjellqvist 4.228
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.706
Solomon 3.961
Lehninger 3.923
Nozaki 4.113
DTASelect 4.329
Thurlkill 3.859
EMBOSS 3.948
Sillero 4.113
Patrickios 4.202
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 4.054
Protein with the highest isoelectric point:
>tr|A0A4D5ZX01|A0A4D5ZX01_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan761 OX=2558857 GN=JavanS761_0011 PE=4 SV=1
MM1 pKa = 7.87 KK2 pKa = 9.27 ITEE5 pKa = 4.15 YY6 pKa = 10.16 TKK8 pKa = 10.79 KK9 pKa = 10.58 DD10 pKa = 2.93 GSTVYY15 pKa = 10.37 RR16 pKa = 11.84 SSVYY20 pKa = 10.68 LGIDD24 pKa = 3.15 TVTGKK29 pKa = 10.24 KK30 pKa = 10.27 VKK32 pKa = 8.07 TTISGRR38 pKa = 11.84 TKK40 pKa = 10.61 RR41 pKa = 11.84 EE42 pKa = 3.76 LKK44 pKa = 10.7 AKK46 pKa = 10.16 ALQAQIDD53 pKa = 4.0 FEE55 pKa = 5.43 KK56 pKa = 11.01 DD57 pKa = 2.61 GSTVYY62 pKa = 9.19 KK63 pKa = 9.51 TVEE66 pKa = 3.56 IKK68 pKa = 9.81 TYY70 pKa = 11.22 AEE72 pKa = 4.37 LVEE75 pKa = 4.62 NWLEE79 pKa = 4.57 TYY81 pKa = 10.41 CHH83 pKa = 5.52 TVKK86 pKa = 10.68 KK87 pKa = 8.6 STLMGTKK94 pKa = 10.34 FKK96 pKa = 10.22 IDD98 pKa = 3.75 KK99 pKa = 10.11 YY100 pKa = 10.77 LLPAFGNYY108 pKa = 9.89 RR109 pKa = 11.84 LDD111 pKa = 3.59 KK112 pKa = 9.28 LTPPIIQKK120 pKa = 9.18 QVNQWAKK127 pKa = 10.93 DD128 pKa = 3.66 YY129 pKa = 10.61 NQLGKK134 pKa = 10.78 GFQEE138 pKa = 4.1 YY139 pKa = 9.86 PLLHH143 pKa = 6.44 SLNKK147 pKa = 10.22 RR148 pKa = 11.84 ILKK151 pKa = 9.85 YY152 pKa = 10.39 AVSLQAIPFNPARR165 pKa = 11.84 DD166 pKa = 3.81 VIVPRR171 pKa = 11.84 RR172 pKa = 11.84 KK173 pKa = 9.67 EE174 pKa = 3.77 KK175 pKa = 10.62 EE176 pKa = 4.12 GQKK179 pKa = 10.54 LKK181 pKa = 11.2 YY182 pKa = 10.43 LDD184 pKa = 4.8 DD185 pKa = 5.74 DD186 pKa = 4.0 NLKK189 pKa = 10.77 KK190 pKa = 10.58 FLTYY194 pKa = 10.66 LEE196 pKa = 4.38 QLPNTYY202 pKa = 10.78 KK203 pKa = 10.95 NFFDD207 pKa = 3.54 TVLYY211 pKa = 8.16 KK212 pKa = 10.46 TLLATGLRR220 pKa = 11.84 IRR222 pKa = 11.84 EE223 pKa = 4.14 CLALEE228 pKa = 4.24 WSDD231 pKa = 3.93 IDD233 pKa = 5.0 LKK235 pKa = 11.48 NGTLDD240 pKa = 3.4 VNKK243 pKa = 9.12 TLNIIKK249 pKa = 10.08 EE250 pKa = 3.87 ITGPKK255 pKa = 7.9 TKK257 pKa = 10.51 SSVRR261 pKa = 11.84 MIDD264 pKa = 3.65 LDD266 pKa = 3.84 NKK268 pKa = 8.1 TVLMLRR274 pKa = 11.84 LYY276 pKa = 10.4 KK277 pKa = 10.37 ARR279 pKa = 11.84 QSQIGKK285 pKa = 9.97 EE286 pKa = 3.77 MGLTYY291 pKa = 10.69 EE292 pKa = 4.58 KK293 pKa = 10.85 VFSNSFDD300 pKa = 3.22 KK301 pKa = 11.33 HH302 pKa = 6.18 IDD304 pKa = 2.98 ARR306 pKa = 11.84 MLSFRR311 pKa = 11.84 LVKK314 pKa = 10.02 HH315 pKa = 6.13 LEE317 pKa = 3.98 RR318 pKa = 11.84 AGCPRR323 pKa = 11.84 FTFHH327 pKa = 7.93 AFRR330 pKa = 11.84 HH331 pKa = 4.53 THH333 pKa = 6.71 ASILLNAGLPYY344 pKa = 10.43 KK345 pKa = 10.2 EE346 pKa = 3.68 IQTRR350 pKa = 11.84 LGHH353 pKa = 6.71 AKK355 pKa = 10.54 LSMTMDD361 pKa = 3.71 IYY363 pKa = 11.79 SHH365 pKa = 7.11 LSKK368 pKa = 10.95 DD369 pKa = 3.54 NQKK372 pKa = 10.5 NATSFYY378 pKa = 10.02 EE379 pKa = 3.99 KK380 pKa = 10.33 AIEE383 pKa = 4.04 KK384 pKa = 10.35 LKK386 pKa = 11.16 SSS388 pKa = 3.82
Molecular weight: 44.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.009
IPC2_protein 9.151
IPC_protein 9.004
Toseland 9.867
ProMoST 9.516
Dawson 10.072
Bjellqvist 9.706
Wikipedia 10.218
Rodwell 10.599
Grimsley 10.131
Solomon 10.072
Lehninger 10.043
Nozaki 9.853
DTASelect 9.706
Thurlkill 9.911
EMBOSS 10.262
Sillero 9.97
Patrickios 9.984
IPC_peptide 10.087
IPC2_peptide 8.17
IPC2.peptide.svr19 8.08
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
29
0
29
4161
46
518
143.5
16.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.768 ± 0.404
0.625 ± 0.104
5.696 ± 0.401
9.349 ± 0.634
4.158 ± 0.276
4.278 ± 0.305
1.61 ± 0.263
7.594 ± 0.423
8.868 ± 0.607
10.214 ± 0.475
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.547 ± 0.304
5.84 ± 0.552
2.86 ± 0.35
4.158 ± 0.304
5.263 ± 0.349
5.648 ± 0.287
5.359 ± 0.42
5.071 ± 0.379
0.817 ± 0.187
4.278 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here