Streptococcus satellite phage Javan761

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D6A169|A0A4D6A169_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan761 OX=2558857 GN=JavanS761_0023 PE=4 SV=1
MM1 pKa = 7.22TLDD4 pKa = 4.01LGKK7 pKa = 8.39MTQAEE12 pKa = 4.45FDD14 pKa = 3.72EE15 pKa = 4.66VMADD19 pKa = 3.21IKK21 pKa = 11.13ARR23 pKa = 11.84NPNLLQLITDD33 pKa = 4.52FLDD36 pKa = 3.62RR37 pKa = 11.84KK38 pKa = 8.24VTPEE42 pKa = 4.05EE43 pKa = 3.86VDD45 pKa = 3.54DD46 pKa = 4.24

Molecular weight:
5.28 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZX01|A0A4D5ZX01_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan761 OX=2558857 GN=JavanS761_0011 PE=4 SV=1
MM1 pKa = 7.87KK2 pKa = 9.27ITEE5 pKa = 4.15YY6 pKa = 10.16TKK8 pKa = 10.79KK9 pKa = 10.58DD10 pKa = 2.93GSTVYY15 pKa = 10.37RR16 pKa = 11.84SSVYY20 pKa = 10.68LGIDD24 pKa = 3.15TVTGKK29 pKa = 10.24KK30 pKa = 10.27VKK32 pKa = 8.07TTISGRR38 pKa = 11.84TKK40 pKa = 10.61RR41 pKa = 11.84EE42 pKa = 3.76LKK44 pKa = 10.7AKK46 pKa = 10.16ALQAQIDD53 pKa = 4.0FEE55 pKa = 5.43KK56 pKa = 11.01DD57 pKa = 2.61GSTVYY62 pKa = 9.19KK63 pKa = 9.51TVEE66 pKa = 3.56IKK68 pKa = 9.81TYY70 pKa = 11.22AEE72 pKa = 4.37LVEE75 pKa = 4.62NWLEE79 pKa = 4.57TYY81 pKa = 10.41CHH83 pKa = 5.52TVKK86 pKa = 10.68KK87 pKa = 8.6STLMGTKK94 pKa = 10.34FKK96 pKa = 10.22IDD98 pKa = 3.75KK99 pKa = 10.11YY100 pKa = 10.77LLPAFGNYY108 pKa = 9.89RR109 pKa = 11.84LDD111 pKa = 3.59KK112 pKa = 9.28LTPPIIQKK120 pKa = 9.18QVNQWAKK127 pKa = 10.93DD128 pKa = 3.66YY129 pKa = 10.61NQLGKK134 pKa = 10.78GFQEE138 pKa = 4.1YY139 pKa = 9.86PLLHH143 pKa = 6.44SLNKK147 pKa = 10.22RR148 pKa = 11.84ILKK151 pKa = 9.85YY152 pKa = 10.39AVSLQAIPFNPARR165 pKa = 11.84DD166 pKa = 3.81VIVPRR171 pKa = 11.84RR172 pKa = 11.84KK173 pKa = 9.67EE174 pKa = 3.77KK175 pKa = 10.62EE176 pKa = 4.12GQKK179 pKa = 10.54LKK181 pKa = 11.2YY182 pKa = 10.43LDD184 pKa = 4.8DD185 pKa = 5.74DD186 pKa = 4.0NLKK189 pKa = 10.77KK190 pKa = 10.58FLTYY194 pKa = 10.66LEE196 pKa = 4.38QLPNTYY202 pKa = 10.78KK203 pKa = 10.95NFFDD207 pKa = 3.54TVLYY211 pKa = 8.16KK212 pKa = 10.46TLLATGLRR220 pKa = 11.84IRR222 pKa = 11.84EE223 pKa = 4.14CLALEE228 pKa = 4.24WSDD231 pKa = 3.93IDD233 pKa = 5.0LKK235 pKa = 11.48NGTLDD240 pKa = 3.4VNKK243 pKa = 9.12TLNIIKK249 pKa = 10.08EE250 pKa = 3.87ITGPKK255 pKa = 7.9TKK257 pKa = 10.51SSVRR261 pKa = 11.84MIDD264 pKa = 3.65LDD266 pKa = 3.84NKK268 pKa = 8.1TVLMLRR274 pKa = 11.84LYY276 pKa = 10.4KK277 pKa = 10.37ARR279 pKa = 11.84QSQIGKK285 pKa = 9.97EE286 pKa = 3.77MGLTYY291 pKa = 10.69EE292 pKa = 4.58KK293 pKa = 10.85VFSNSFDD300 pKa = 3.22KK301 pKa = 11.33HH302 pKa = 6.18IDD304 pKa = 2.98ARR306 pKa = 11.84MLSFRR311 pKa = 11.84LVKK314 pKa = 10.02HH315 pKa = 6.13LEE317 pKa = 3.98RR318 pKa = 11.84AGCPRR323 pKa = 11.84FTFHH327 pKa = 7.93AFRR330 pKa = 11.84HH331 pKa = 4.53THH333 pKa = 6.71ASILLNAGLPYY344 pKa = 10.43KK345 pKa = 10.2EE346 pKa = 3.68IQTRR350 pKa = 11.84LGHH353 pKa = 6.71AKK355 pKa = 10.54LSMTMDD361 pKa = 3.71IYY363 pKa = 11.79SHH365 pKa = 7.11LSKK368 pKa = 10.95DD369 pKa = 3.54NQKK372 pKa = 10.5NATSFYY378 pKa = 10.02EE379 pKa = 3.99KK380 pKa = 10.33AIEE383 pKa = 4.04KK384 pKa = 10.35LKK386 pKa = 11.16SSS388 pKa = 3.82

Molecular weight:
44.88 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29

0

29

4161

46

518

143.5

16.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.768 ± 0.404

0.625 ± 0.104

5.696 ± 0.401

9.349 ± 0.634

4.158 ± 0.276

4.278 ± 0.305

1.61 ± 0.263

7.594 ± 0.423

8.868 ± 0.607

10.214 ± 0.475

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.304

5.84 ± 0.552

2.86 ± 0.35

4.158 ± 0.304

5.263 ± 0.349

5.648 ± 0.287

5.359 ± 0.42

5.071 ± 0.379

0.817 ± 0.187

4.278 ± 0.302

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski