Oceaniovalibus sp. ACAM 378
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4789 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5D0S5S7|A0A5D0S5S7_9RHOB ABC transporter ATP-binding protein OS=Oceaniovalibus sp. ACAM 378 OX=2599923 GN=FQ320_09020 PE=4 SV=1
MM1 pKa = 6.92 TAEE4 pKa = 4.03 NSAPMEE10 pKa = 4.47 GAPLIQPSSTDD21 pKa = 2.96 HH22 pKa = 6.93 PLYY25 pKa = 10.82 DD26 pKa = 4.46 DD27 pKa = 3.81 VVGACRR33 pKa = 11.84 SVYY36 pKa = 10.59 DD37 pKa = 3.78 PEE39 pKa = 5.06 IPVNIFDD46 pKa = 5.17 LGLIYY51 pKa = 10.38 TIDD54 pKa = 3.49 INGEE58 pKa = 3.89 NHH60 pKa = 5.88 VEE62 pKa = 3.7 ILMSLTAPGCPVAGEE77 pKa = 4.13 MPGWIADD84 pKa = 4.29 AIEE87 pKa = 4.18 PLAGVKK93 pKa = 9.76 SVNVEE98 pKa = 4.27 LTWSPPWGMEE108 pKa = 3.74 MMSDD112 pKa = 3.74 EE113 pKa = 4.65 ARR115 pKa = 11.84 LEE117 pKa = 4.14 LGFMM121 pKa = 4.54
Molecular weight: 13.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.795
IPC_protein 3.706
Toseland 3.528
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.605
Rodwell 3.554
Grimsley 3.452
Solomon 3.668
Lehninger 3.617
Nozaki 3.821
DTASelect 3.961
Thurlkill 3.579
EMBOSS 3.617
Sillero 3.834
Patrickios 0.769
IPC_peptide 3.668
IPC2_peptide 3.808
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|A0A5D0RX24|A0A5D0RX24_9RHOB Cold-shock protein OS=Oceaniovalibus sp. ACAM 378 OX=2599923 GN=FQ320_18805 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 KK14 pKa = 8.83 HH15 pKa = 4.64 RR16 pKa = 11.84 HH17 pKa = 3.91 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.08 AGRR29 pKa = 11.84 RR30 pKa = 11.84 ILNARR35 pKa = 11.84 RR36 pKa = 11.84 ARR38 pKa = 11.84 GRR40 pKa = 11.84 KK41 pKa = 9.07 SLSAA45 pKa = 3.93
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4789
0
4789
1539245
17
6671
321.4
34.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.288 ± 0.044
0.905 ± 0.01
6.14 ± 0.031
5.288 ± 0.03
3.773 ± 0.025
8.793 ± 0.039
2.048 ± 0.016
5.392 ± 0.023
2.861 ± 0.026
10.161 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.775 ± 0.016
2.59 ± 0.019
5.222 ± 0.026
3.086 ± 0.02
6.874 ± 0.034
5.278 ± 0.023
5.738 ± 0.022
7.336 ± 0.032
1.403 ± 0.013
2.049 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here