Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Chromobacteriaceae; Chromobacterium group; Chromobacterium; Chromobacterium violaceum

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4397 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7NYY8|Q7NYY8_CHRVO Uncharacterized protein OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) OX=243365 GN=CV_1134 PE=4 SV=1
MM1 pKa = 7.36PTLHH5 pKa = 6.65PQADD9 pKa = 4.21AHH11 pKa = 5.87WVMAIPVLSTSHH23 pKa = 6.68IRR25 pKa = 11.84QQTHH29 pKa = 5.01AWLQINVGGWIALEE43 pKa = 3.91YY44 pKa = 11.03AEE46 pKa = 4.58YY47 pKa = 11.02SHH49 pKa = 6.81GFFIALGEE57 pKa = 4.22SDD59 pKa = 4.54SDD61 pKa = 3.87PAEE64 pKa = 4.26WFPDD68 pKa = 3.74HH69 pKa = 7.81PDD71 pKa = 3.49LQHH74 pKa = 5.79IASWVKK80 pKa = 10.46SNCPAATWMRR90 pKa = 11.84LDD92 pKa = 3.98ADD94 pKa = 4.06GDD96 pKa = 4.2VIDD99 pKa = 5.57ALPSYY104 pKa = 10.48DD105 pKa = 3.47WEE107 pKa = 4.45EE108 pKa = 4.08GDD110 pKa = 5.14RR111 pKa = 11.84DD112 pKa = 3.86SQAATGSTVPSEE124 pKa = 4.15SLAILVVISGGEE136 pKa = 3.95VEE138 pKa = 5.77GVYY141 pKa = 10.59ASQGDD146 pKa = 4.41CEE148 pKa = 4.51VKK150 pKa = 10.48IVDD153 pKa = 5.54LDD155 pKa = 4.26DD156 pKa = 5.85ADD158 pKa = 5.56DD159 pKa = 4.3DD160 pKa = 5.05AEE162 pKa = 4.18YY163 pKa = 10.79FPLSGFADD171 pKa = 3.45QTAFAAYY178 pKa = 9.53RR179 pKa = 11.84DD180 pKa = 3.71QLHH183 pKa = 6.24CVRR186 pKa = 5.28

Molecular weight: 20.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q7NPZ7|MURD_CHRVO UDP-N-acetylmuramoylalanine--D-glutamate ligase OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) OX=243365 GN=murD PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.31QPSTTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.24RR14 pKa = 11.84THH16 pKa = 5.92GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.8GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4397

0

4397

1395434

37

4130

317.4

34.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.493 ± 0.06

1.03 ± 0.014

5.445 ± 0.035

5.379 ± 0.038

3.441 ± 0.026

8.401 ± 0.042

2.188 ± 0.017

4.417 ± 0.028

3.59 ± 0.032

11.495 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.455 ± 0.021

2.867 ± 0.035

4.958 ± 0.036

4.388 ± 0.038

6.867 ± 0.05

5.645 ± 0.036

4.247 ± 0.038

6.746 ± 0.039

1.468 ± 0.02

2.483 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski