Hebeloma cylindrosporum h7

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Cortinariaceae; Hebeloma; Hebeloma cylindrosporum

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15327 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C3C094|A0A0C3C094_HEBCY Uncharacterized protein OS=Hebeloma cylindrosporum h7 OX=686832 GN=M413DRAFT_253727 PE=4 SV=1
MM1 pKa = 6.9NQSGLATTTEE11 pKa = 4.2EE12 pKa = 4.26DD13 pKa = 3.81TVVAIIRR20 pKa = 11.84AMQALGLNVCLAADD34 pKa = 4.15FQGDD38 pKa = 3.99VPSLVARR45 pKa = 11.84LDD47 pKa = 3.3AAMQHH52 pKa = 5.07QLARR56 pKa = 11.84HH57 pKa = 6.08LEE59 pKa = 3.96ISSQQDD65 pKa = 3.26SAVASIAEE73 pKa = 4.03RR74 pKa = 11.84RR75 pKa = 11.84TEE77 pKa = 3.96DD78 pKa = 4.96SEE80 pKa = 5.03DD81 pKa = 3.73ADD83 pKa = 3.28ITTDD87 pKa = 3.55EE88 pKa = 4.36EE89 pKa = 4.32SQAIAVAQALDD100 pKa = 3.84YY101 pKa = 10.79LAEE104 pKa = 4.35EE105 pKa = 4.48ATQAADD111 pKa = 3.33LEE113 pKa = 4.43ARR115 pKa = 11.84FLEE118 pKa = 4.3NEE120 pKa = 3.96EE121 pKa = 4.37LEE123 pKa = 4.44EE124 pKa = 4.99LEE126 pKa = 4.47RR127 pKa = 11.84APDD130 pKa = 3.25RR131 pKa = 11.84WAHH134 pKa = 6.3LDD136 pKa = 3.13WSLDD140 pKa = 3.44DD141 pKa = 4.29MSYY144 pKa = 11.5DD145 pKa = 4.41PYY147 pKa = 11.63NSDD150 pKa = 5.56DD151 pKa = 4.29DD152 pKa = 3.99QCGCYY157 pKa = 9.99SSYY160 pKa = 11.54GKK162 pKa = 10.31DD163 pKa = 3.29DD164 pKa = 4.47DD165 pKa = 5.1GEE167 pKa = 4.53VVHH170 pKa = 6.55YY171 pKa = 10.49DD172 pKa = 3.49CEE174 pKa = 4.45YY175 pKa = 10.72SS176 pKa = 3.25

Molecular weight: 19.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C2Y8T9|A0A0C2Y8T9_HEBCY DUF302 domain-containing protein OS=Hebeloma cylindrosporum h7 OX=686832 GN=M413DRAFT_441321 PE=4 SV=1
RR1 pKa = 7.81GGAKK5 pKa = 10.04KK6 pKa = 9.75PVAVRR11 pKa = 11.84GGAKK15 pKa = 10.02KK16 pKa = 9.78PVAVRR21 pKa = 11.84GGAKK25 pKa = 10.02KK26 pKa = 9.78PVAVRR31 pKa = 11.84GGAKK35 pKa = 9.81KK36 pKa = 9.48PAAVRR41 pKa = 11.84GGAKK45 pKa = 10.02KK46 pKa = 9.78PVAVRR51 pKa = 11.84GGAKK55 pKa = 9.81KK56 pKa = 9.48PAAVRR61 pKa = 11.84GGAKK65 pKa = 9.8KK66 pKa = 9.48PAAVRR71 pKa = 11.84GGAKK75 pKa = 9.87KK76 pKa = 9.41PASVRR81 pKa = 11.84GGSKK85 pKa = 9.5PAAVRR90 pKa = 11.84GG91 pKa = 3.77

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15324

3

15327

5907033

49

5548

385.4

42.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.856 ± 0.017

1.262 ± 0.008

5.474 ± 0.012

5.843 ± 0.016

4.002 ± 0.012

6.331 ± 0.019

2.626 ± 0.01

5.213 ± 0.014

4.727 ± 0.019

9.38 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.063 ± 0.007

3.674 ± 0.011

6.681 ± 0.025

3.698 ± 0.013

6.16 ± 0.016

8.887 ± 0.028

5.954 ± 0.015

6.099 ± 0.014

1.414 ± 0.008

2.656 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski