Croton yellow vein mosaic alphasatellite
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D3HTY0|D3HTY0_9VIRU Replication protein OS=Croton yellow vein mosaic alphasatellite OX=713089 GN=alpha rep PE=4 SV=1
MM1 pKa = 6.91 SHH3 pKa = 5.9 SRR5 pKa = 11.84 NWCFTIFNYY14 pKa = 9.97 VLPLFTTLPEE24 pKa = 3.79 WANYY28 pKa = 10.27 LVFQEE33 pKa = 4.98 EE34 pKa = 4.62 EE35 pKa = 4.36 CPTTKK40 pKa = 9.85 KK41 pKa = 10.48 RR42 pKa = 11.84 HH43 pKa = 4.56 IQGYY47 pKa = 9.82 VNLKK51 pKa = 9.19 RR52 pKa = 11.84 NQRR55 pKa = 11.84 FAFLKK60 pKa = 10.48 KK61 pKa = 9.96 KK62 pKa = 10.7 LPDD65 pKa = 3.85 GSHH68 pKa = 6.86 IEE70 pKa = 4.0 PCRR73 pKa = 11.84 GSASSNRR80 pKa = 11.84 DD81 pKa = 3.15 YY82 pKa = 10.47 CTKK85 pKa = 10.47 DD86 pKa = 2.88 ASRR89 pKa = 11.84 TGGPWEE95 pKa = 4.17 FGVFCEE101 pKa = 4.38 TGSNKK106 pKa = 9.76 RR107 pKa = 11.84 KK108 pKa = 7.94 TMEE111 pKa = 4.3 RR112 pKa = 11.84 FQEE115 pKa = 4.28 DD116 pKa = 4.08 PEE118 pKa = 4.17 EE119 pKa = 4.61 LRR121 pKa = 11.84 LADD124 pKa = 3.63 PKK126 pKa = 10.93 LYY128 pKa = 10.25 RR129 pKa = 11.84 RR130 pKa = 11.84 CLATKK135 pKa = 10.51 VNTEE139 pKa = 3.71 FSGLVLPVPDD149 pKa = 5.19 RR150 pKa = 11.84 PWQLVAQKK158 pKa = 10.95 ALDD161 pKa = 3.56 QGPDD165 pKa = 3.18 DD166 pKa = 4.05 RR167 pKa = 11.84 TIIWVYY173 pKa = 10.5 GSEE176 pKa = 4.1 GNEE179 pKa = 4.89 GKK181 pKa = 7.15 TTWAKK186 pKa = 10.56 IKK188 pKa = 10.3 IQEE191 pKa = 3.75 GWFYY195 pKa = 11.47 SRR197 pKa = 11.84 GGKK200 pKa = 9.87 GEE202 pKa = 4.13 NIKK205 pKa = 10.35 YY206 pKa = 10.12 QYY208 pKa = 10.88 AEE210 pKa = 4.16 HH211 pKa = 6.98 LGHH214 pKa = 6.82 CVFDD218 pKa = 3.66 IPRR221 pKa = 11.84 QVEE224 pKa = 4.24 DD225 pKa = 3.31 NLQYY229 pKa = 10.79 TVLEE233 pKa = 4.5 EE234 pKa = 3.95 IKK236 pKa = 10.78 DD237 pKa = 3.69 RR238 pKa = 11.84 LIRR241 pKa = 11.84 SSKK244 pKa = 10.56 YY245 pKa = 10.02 EE246 pKa = 4.51 PIDD249 pKa = 3.78 FNCSDD254 pKa = 3.65 KK255 pKa = 11.19 VHH257 pKa = 6.57 LVVLSNFLPCLDD269 pKa = 3.29 VEE271 pKa = 4.56 YY272 pKa = 10.84 NNRR275 pKa = 11.84 GEE277 pKa = 4.25 LVKK280 pKa = 10.8 KK281 pKa = 10.14 PLLSRR286 pKa = 11.84 DD287 pKa = 2.91 RR288 pKa = 11.84 VFIINIDD295 pKa = 3.63 EE296 pKa = 4.59 SVCGHH301 pKa = 7.3 PDD303 pKa = 3.24 DD304 pKa = 5.56 LKK306 pKa = 11.53 NFDD309 pKa = 4.28 VYY311 pKa = 11.54 LEE313 pKa = 4.05
Molecular weight: 36.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.162
IPC2_protein 6.237
IPC_protein 6.313
Toseland 6.389
ProMoST 6.517
Dawson 6.707
Bjellqvist 6.678
Wikipedia 6.693
Rodwell 6.693
Grimsley 6.465
Solomon 6.707
Lehninger 6.707
Nozaki 6.985
DTASelect 7.088
Thurlkill 7.117
EMBOSS 7.102
Sillero 7.102
Patrickios 4.164
IPC_peptide 6.722
IPC2_peptide 6.825
IPC2.peptide.svr19 6.785
Protein with the highest isoelectric point:
>tr|D3HTY0|D3HTY0_9VIRU Replication protein OS=Croton yellow vein mosaic alphasatellite OX=713089 GN=alpha rep PE=4 SV=1
MM1 pKa = 6.91 SHH3 pKa = 5.9 SRR5 pKa = 11.84 NWCFTIFNYY14 pKa = 9.97 VLPLFTTLPEE24 pKa = 3.79 WANYY28 pKa = 10.27 LVFQEE33 pKa = 4.98 EE34 pKa = 4.62 EE35 pKa = 4.36 CPTTKK40 pKa = 9.85 KK41 pKa = 10.48 RR42 pKa = 11.84 HH43 pKa = 4.56 IQGYY47 pKa = 9.82 VNLKK51 pKa = 9.19 RR52 pKa = 11.84 NQRR55 pKa = 11.84 FAFLKK60 pKa = 10.48 KK61 pKa = 9.96 KK62 pKa = 10.7 LPDD65 pKa = 3.85 GSHH68 pKa = 6.86 IEE70 pKa = 4.0 PCRR73 pKa = 11.84 GSASSNRR80 pKa = 11.84 DD81 pKa = 3.15 YY82 pKa = 10.47 CTKK85 pKa = 10.47 DD86 pKa = 2.88 ASRR89 pKa = 11.84 TGGPWEE95 pKa = 4.17 FGVFCEE101 pKa = 4.38 TGSNKK106 pKa = 9.76 RR107 pKa = 11.84 KK108 pKa = 7.94 TMEE111 pKa = 4.3 RR112 pKa = 11.84 FQEE115 pKa = 4.28 DD116 pKa = 4.08 PEE118 pKa = 4.17 EE119 pKa = 4.61 LRR121 pKa = 11.84 LADD124 pKa = 3.63 PKK126 pKa = 10.93 LYY128 pKa = 10.25 RR129 pKa = 11.84 RR130 pKa = 11.84 CLATKK135 pKa = 10.51 VNTEE139 pKa = 3.71 FSGLVLPVPDD149 pKa = 5.19 RR150 pKa = 11.84 PWQLVAQKK158 pKa = 10.95 ALDD161 pKa = 3.56 QGPDD165 pKa = 3.18 DD166 pKa = 4.05 RR167 pKa = 11.84 TIIWVYY173 pKa = 10.5 GSEE176 pKa = 4.1 GNEE179 pKa = 4.89 GKK181 pKa = 7.15 TTWAKK186 pKa = 10.56 IKK188 pKa = 10.3 IQEE191 pKa = 3.75 GWFYY195 pKa = 11.47 SRR197 pKa = 11.84 GGKK200 pKa = 9.87 GEE202 pKa = 4.13 NIKK205 pKa = 10.35 YY206 pKa = 10.12 QYY208 pKa = 10.88 AEE210 pKa = 4.16 HH211 pKa = 6.98 LGHH214 pKa = 6.82 CVFDD218 pKa = 3.66 IPRR221 pKa = 11.84 QVEE224 pKa = 4.24 DD225 pKa = 3.31 NLQYY229 pKa = 10.79 TVLEE233 pKa = 4.5 EE234 pKa = 3.95 IKK236 pKa = 10.78 DD237 pKa = 3.69 RR238 pKa = 11.84 LIRR241 pKa = 11.84 SSKK244 pKa = 10.56 YY245 pKa = 10.02 EE246 pKa = 4.51 PIDD249 pKa = 3.78 FNCSDD254 pKa = 3.65 KK255 pKa = 11.19 VHH257 pKa = 6.57 LVVLSNFLPCLDD269 pKa = 3.29 VEE271 pKa = 4.56 YY272 pKa = 10.84 NNRR275 pKa = 11.84 GEE277 pKa = 4.25 LVKK280 pKa = 10.8 KK281 pKa = 10.14 PLLSRR286 pKa = 11.84 DD287 pKa = 2.91 RR288 pKa = 11.84 VFIINIDD295 pKa = 3.63 EE296 pKa = 4.59 SVCGHH301 pKa = 7.3 PDD303 pKa = 3.24 DD304 pKa = 5.56 LKK306 pKa = 11.53 NFDD309 pKa = 4.28 VYY311 pKa = 11.54 LEE313 pKa = 4.05
Molecular weight: 36.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.162
IPC2_protein 6.237
IPC_protein 6.313
Toseland 6.389
ProMoST 6.517
Dawson 6.707
Bjellqvist 6.678
Wikipedia 6.693
Rodwell 6.693
Grimsley 6.465
Solomon 6.707
Lehninger 6.707
Nozaki 6.985
DTASelect 7.088
Thurlkill 7.117
EMBOSS 7.102
Sillero 7.102
Patrickios 4.164
IPC_peptide 6.722
IPC2_peptide 6.825
IPC2.peptide.svr19 6.785
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
313
313
313
313.0
36.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.195 ± 0.0
3.195 ± 0.0
6.39 ± 0.0
7.987 ± 0.0
5.112 ± 0.0
6.07 ± 0.0
2.236 ± 0.0
4.792 ± 0.0
7.348 ± 0.0
8.946 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
0.639 ± 0.0
5.431 ± 0.0
5.431 ± 0.0
3.514 ± 0.0
6.709 ± 0.0
5.431 ± 0.0
4.792 ± 0.0
6.39 ± 0.0
2.236 ± 0.0
4.153 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here