Escherichia phage SUSP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; Suspvirus; Escherichia virus SUSP1

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 138 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9RSH0|A0A0N9RSH0_9CAUD Uncharacterized protein OS=Escherichia phage SUSP1 OX=1718606 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.8CFHH5 pKa = 6.61GTTQEE10 pKa = 3.9NFLNLINGGEE20 pKa = 4.22KK21 pKa = 10.27PSGAWNCSDD30 pKa = 3.88MDD32 pKa = 3.81GCFYY36 pKa = 11.02VYY38 pKa = 10.04PVNKK42 pKa = 10.0FYY44 pKa = 11.07DD45 pKa = 3.71VEE47 pKa = 4.9DD48 pKa = 5.1LDD50 pKa = 5.52DD51 pKa = 4.08EE52 pKa = 4.78QITSEE57 pKa = 4.47GIRR60 pKa = 11.84NALDD64 pKa = 3.43SASITAAYY72 pKa = 9.41QMKK75 pKa = 9.49SQKK78 pKa = 10.0IVILEE83 pKa = 3.9LDD85 pKa = 3.49IPEE88 pKa = 5.38EE89 pKa = 4.2DD90 pKa = 4.98LQDD93 pKa = 3.74DD94 pKa = 4.5WSCEE98 pKa = 3.85NMSDD102 pKa = 3.41VASFTEE108 pKa = 4.31YY109 pKa = 10.6FDD111 pKa = 4.59KK112 pKa = 11.09EE113 pKa = 4.51WIKK116 pKa = 10.8KK117 pKa = 9.87IYY119 pKa = 6.98TTEE122 pKa = 3.99FNGMYY127 pKa = 10.57APFFVPNLNNRR138 pKa = 11.84NLGYY142 pKa = 10.27IPDD145 pKa = 3.95EE146 pKa = 4.23LRR148 pKa = 11.84NIAAMIQRR156 pKa = 11.84SDD158 pKa = 3.27EE159 pKa = 4.15MNNVYY164 pKa = 10.21IDD166 pKa = 3.38IFEE169 pKa = 4.3TMQTYY174 pKa = 10.19VSEE177 pKa = 4.26TSISDD182 pKa = 3.46LTAA185 pKa = 4.81

Molecular weight: 21.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N7GFL1|A0A0N7GFL1_9CAUD Uncharacterized protein OS=Escherichia phage SUSP1 OX=1718606 PE=4 SV=1
MM1 pKa = 7.83GIFSNMKK8 pKa = 10.18AGLLAALAKK17 pKa = 10.2AAAIVSMTGKK27 pKa = 9.96QVGVDD32 pKa = 3.75ASAVAQVFASQIGQQPYY49 pKa = 8.63IHH51 pKa = 6.6VGRR54 pKa = 11.84GKK56 pKa = 10.52GGKK59 pKa = 8.68KK60 pKa = 8.45QAHH63 pKa = 5.92RR64 pKa = 11.84QTGAAAIKK72 pKa = 10.1RR73 pKa = 11.84AAKK76 pKa = 9.87KK77 pKa = 9.93ARR79 pKa = 11.84NRR81 pKa = 11.84KK82 pKa = 8.88RR83 pKa = 11.84SKK85 pKa = 10.72

Molecular weight: 8.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

138

0

138

26755

29

899

193.9

21.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.307 ± 0.26

1.346 ± 0.107

6.227 ± 0.169

6.761 ± 0.203

4.302 ± 0.141

6.533 ± 0.209

1.996 ± 0.116

5.92 ± 0.137

7.842 ± 0.273

7.763 ± 0.166

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.994 ± 0.111

5.18 ± 0.144

2.968 ± 0.134

3.64 ± 0.144

4.298 ± 0.14

6.234 ± 0.192

6.32 ± 0.261

6.817 ± 0.159

1.174 ± 0.099

4.377 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski