Streptococcus satellite phage Javan538
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZQA2|A0A4D5ZQA2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan538 OX=2558732 GN=JavanS538_0006 PE=4 SV=1
MM1 pKa = 7.46 TEE3 pKa = 3.55 NHH5 pKa = 6.21 YY6 pKa = 10.99 LEE8 pKa = 4.44 QAEE11 pKa = 4.29 KK12 pKa = 10.96 DD13 pKa = 3.61 RR14 pKa = 11.84 LEE16 pKa = 4.75 LEE18 pKa = 4.25 QHH20 pKa = 6.33 HH21 pKa = 7.08 LNYY24 pKa = 9.99 MADD27 pKa = 3.77 DD28 pKa = 3.97 TPIYY32 pKa = 9.65 PSNIPKK38 pKa = 10.44 LMEE41 pKa = 4.03 IAKK44 pKa = 9.99 KK45 pKa = 10.13 LQAEE49 pKa = 4.2 DD50 pKa = 3.33 TSLNIYY56 pKa = 9.2 EE57 pKa = 5.15 LYY59 pKa = 9.95 KK60 pKa = 10.61 QPEE63 pKa = 4.11 ARR65 pKa = 11.84 TKK67 pKa = 10.57 LFSQITEE74 pKa = 3.83 ACYY77 pKa = 9.19 MALSATPTQAQRR89 pKa = 11.84 LAFCDD94 pKa = 3.79 YY95 pKa = 11.34 LEE97 pKa = 4.08 QQYY100 pKa = 11.5 EE101 pKa = 4.16 NTLKK105 pKa = 11.01 KK106 pKa = 10.21 MIASTDD112 pKa = 3.41 KK113 pKa = 10.77 QALGEE118 pKa = 4.11 LLDD121 pKa = 4.47 LLEE124 pKa = 5.0 LPAEE128 pKa = 4.35 IEE130 pKa = 4.32 SQFIRR135 pKa = 11.84 DD136 pKa = 3.75 MAVSGILAKK145 pKa = 10.63 DD146 pKa = 3.18
Molecular weight: 16.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.585
IPC2_protein 4.66
IPC_protein 4.558
Toseland 4.418
ProMoST 4.584
Dawson 4.495
Bjellqvist 4.673
Wikipedia 4.342
Rodwell 4.406
Grimsley 4.329
Solomon 4.495
Lehninger 4.444
Nozaki 4.609
DTASelect 4.724
Thurlkill 4.418
EMBOSS 4.368
Sillero 4.673
Patrickios 3.579
IPC_peptide 4.507
IPC2_peptide 4.66
IPC2.peptide.svr19 4.632
Protein with the highest isoelectric point:
>tr|A0A4D5ZPR2|A0A4D5ZPR2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan538 OX=2558732 GN=JavanS538_0011 PE=4 SV=1
MM1 pKa = 7.78 KK2 pKa = 10.26 INEE5 pKa = 4.22 IKK7 pKa = 10.67 KK8 pKa = 9.76 KK9 pKa = 10.54 DD10 pKa = 3.3 GSTVYY15 pKa = 10.36 RR16 pKa = 11.84 ANVYY20 pKa = 10.59 LGVDD24 pKa = 4.14 VITGKK29 pKa = 10.74 KK30 pKa = 7.55 VTTKK34 pKa = 9.32 VTARR38 pKa = 11.84 TKK40 pKa = 10.91 KK41 pKa = 9.48 EE42 pKa = 4.24 LKK44 pKa = 9.67 TKK46 pKa = 10.1 AQQAQFDD53 pKa = 4.54 FKK55 pKa = 11.59 ANGSTRR61 pKa = 11.84 YY62 pKa = 9.46 KK63 pKa = 10.03 EE64 pKa = 4.34 VVIEE68 pKa = 4.33 TYY70 pKa = 10.79 EE71 pKa = 3.89 EE72 pKa = 4.27 LAISWWDD79 pKa = 3.38 SYY81 pKa = 11.84 KK82 pKa = 10.19 NTVKK86 pKa = 10.93 ANTRR90 pKa = 11.84 NTQKK94 pKa = 10.88 VLLNNHH100 pKa = 5.76 VLPLFGGFKK109 pKa = 10.16 LDD111 pKa = 4.12 KK112 pKa = 9.92 LTTPLIQSTMNKK124 pKa = 9.46 LANSTNTGEE133 pKa = 4.13 VGAYY137 pKa = 8.68 LHH139 pKa = 6.32 YY140 pKa = 10.97 GKK142 pKa = 9.58 IHH144 pKa = 5.96 TLNKK148 pKa = 10.19 RR149 pKa = 11.84 ILQYY153 pKa = 11.17 GVILQVIPTNPANNVVLPRR172 pKa = 11.84 NTQKK176 pKa = 11.1 DD177 pKa = 3.22 KK178 pKa = 10.56 KK179 pKa = 10.89 AKK181 pKa = 9.81 VKK183 pKa = 10.6 HH184 pKa = 5.76 FNNDD188 pKa = 2.8 EE189 pKa = 4.08 LKK191 pKa = 10.82 QFLTYY196 pKa = 10.53 LDD198 pKa = 4.07 SLDD201 pKa = 3.36 NSKK204 pKa = 11.22 YY205 pKa = 8.53 KK206 pKa = 10.13 TYY208 pKa = 11.4 YY209 pKa = 10.78 DD210 pKa = 2.93 ITLYY214 pKa = 10.96 KK215 pKa = 10.3 FLLATGCRR223 pKa = 11.84 INEE226 pKa = 4.0 ALALSWSDD234 pKa = 2.86 IDD236 pKa = 4.49 LDD238 pKa = 3.79 NSVVHH243 pKa = 5.57 ITKK246 pKa = 9.13 TLNYY250 pKa = 8.52 RR251 pKa = 11.84 KK252 pKa = 9.31 EE253 pKa = 4.21 VNSPKK258 pKa = 10.6 SKK260 pKa = 10.59 AGYY263 pKa = 9.68 RR264 pKa = 11.84 DD265 pKa = 3.11 IDD267 pKa = 3.51 IDD269 pKa = 3.82 QQTATMLKK277 pKa = 9.78 RR278 pKa = 11.84 YY279 pKa = 7.54 QRR281 pKa = 11.84 KK282 pKa = 6.4 QTQEE286 pKa = 3.04 AWKK289 pKa = 10.12 LGRR292 pKa = 11.84 TEE294 pKa = 4.07 TVVFSDD300 pKa = 5.91 FINAYY305 pKa = 8.69 PNYY308 pKa = 9.19 KK309 pKa = 10.04 AVSSRR314 pKa = 11.84 LRR316 pKa = 11.84 THH318 pKa = 6.7 FKK320 pKa = 10.39 KK321 pKa = 10.74 AGVNNIGFHH330 pKa = 5.79 GFRR333 pKa = 11.84 HH334 pKa = 4.87 THH336 pKa = 6.58 ASLLLNSGIPYY347 pKa = 10.0 KK348 pKa = 10.34 EE349 pKa = 3.8 LQHH352 pKa = 6.73 RR353 pKa = 11.84 LGHH356 pKa = 5.68 STLSMTMDD364 pKa = 4.17 TYY366 pKa = 11.8 SHH368 pKa = 7.15 LSKK371 pKa = 11.01 EE372 pKa = 4.3 NAKK375 pKa = 10.31 KK376 pKa = 9.46 ATSFYY381 pKa = 10.79 EE382 pKa = 4.07 KK383 pKa = 10.41 ALKK386 pKa = 10.52 SII388 pKa = 4.29
Molecular weight: 44.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.055
IPC2_protein 9.326
IPC_protein 9.209
Toseland 9.999
ProMoST 9.648
Dawson 10.189
Bjellqvist 9.823
Wikipedia 10.335
Rodwell 10.76
Grimsley 10.262
Solomon 10.204
Lehninger 10.175
Nozaki 9.97
DTASelect 9.823
Thurlkill 10.043
EMBOSS 10.394
Sillero 10.101
Patrickios 10.292
IPC_peptide 10.204
IPC2_peptide 8.273
IPC2.peptide.svr19 8.306
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
2424
62
502
186.5
21.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.941 ± 0.491
0.454 ± 0.103
5.734 ± 0.501
7.508 ± 0.863
3.919 ± 0.616
5.239 ± 0.47
1.691 ± 0.31
6.724 ± 0.593
8.663 ± 0.677
9.158 ± 0.611
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.98 ± 0.327
6.436 ± 0.606
3.837 ± 0.583
4.249 ± 0.66
4.414 ± 0.469
5.652 ± 0.31
6.601 ± 0.699
5.611 ± 0.542
1.073 ± 0.141
5.116 ± 0.448
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here