Amino acid dipepetide frequency for Lentibacillus sp. CBA3610

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.946AlaAla: 5.946 ± 0.101
0.566AlaCys: 0.566 ± 0.026
4.217AlaAsp: 4.217 ± 0.073
5.096AlaGlu: 5.096 ± 0.085
3.396AlaPhe: 3.396 ± 0.063
5.803AlaGly: 5.803 ± 0.089
1.276AlaHis: 1.276 ± 0.035
5.704AlaIle: 5.704 ± 0.073
4.088AlaLys: 4.088 ± 0.065
6.842AlaLeu: 6.842 ± 0.098
2.147AlaMet: 2.147 ± 0.047
2.939AlaAsn: 2.939 ± 0.061
2.057AlaPro: 2.057 ± 0.056
2.153AlaGln: 2.153 ± 0.053
2.719AlaArg: 2.719 ± 0.058
4.3AlaSer: 4.3 ± 0.075
3.277AlaThr: 3.277 ± 0.064
5.469AlaVal: 5.469 ± 0.084
0.645AlaTrp: 0.645 ± 0.03
2.516AlaTyr: 2.516 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
0.368CysAla: 0.368 ± 0.021
0.079CysCys: 0.079 ± 0.01
0.391CysAsp: 0.391 ± 0.021
0.41CysGlu: 0.41 ± 0.025
0.268CysPhe: 0.268 ± 0.016
0.607CysGly: 0.607 ± 0.027
0.206CysHis: 0.206 ± 0.014
0.44CysIle: 0.44 ± 0.019
0.246CysLys: 0.246 ± 0.017
0.567CysLeu: 0.567 ± 0.026
0.187CysMet: 0.187 ± 0.016
0.237CysAsn: 0.237 ± 0.016
0.33CysPro: 0.33 ± 0.02
0.22CysGln: 0.22 ± 0.014
0.312CysArg: 0.312 ± 0.018
0.459CysSer: 0.459 ± 0.023
0.357CysThr: 0.357 ± 0.021
0.355CysVal: 0.355 ± 0.019
0.061CysTrp: 0.061 ± 0.008
0.235CysTyr: 0.235 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.917AspAla: 3.917 ± 0.069
0.328AspCys: 0.328 ± 0.021
3.869AspAsp: 3.869 ± 0.067
5.394AspGlu: 5.394 ± 0.083
2.58AspPhe: 2.58 ± 0.055
3.81AspGly: 3.81 ± 0.07
1.357AspHis: 1.357 ± 0.035
4.603AspIle: 4.603 ± 0.073
3.491AspLys: 3.491 ± 0.066
5.139AspLeu: 5.139 ± 0.086
1.863AspMet: 1.863 ± 0.044
2.66AspAsn: 2.66 ± 0.051
2.18AspPro: 2.18 ± 0.052
2.337AspGln: 2.337 ± 0.046
2.613AspArg: 2.613 ± 0.053
2.969AspSer: 2.969 ± 0.058
2.902AspThr: 2.902 ± 0.057
4.485AspVal: 4.485 ± 0.069
0.683AspTrp: 0.683 ± 0.031
2.508AspTyr: 2.508 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
5.647GluAla: 5.647 ± 0.095
0.317GluCys: 0.317 ± 0.018
4.425GluAsp: 4.425 ± 0.083
6.907GluGlu: 6.907 ± 0.114
2.489GluPhe: 2.489 ± 0.051
4.253GluGly: 4.253 ± 0.075
1.623GluHis: 1.623 ± 0.039
5.144GluIle: 5.144 ± 0.074
5.908GluLys: 5.908 ± 0.092
6.794GluLeu: 6.794 ± 0.102
2.402GluMet: 2.402 ± 0.044
4.394GluAsn: 4.394 ± 0.081
2.244GluPro: 2.244 ± 0.055
3.82GluGln: 3.82 ± 0.077
3.58GluArg: 3.58 ± 0.059
4.012GluSer: 4.012 ± 0.078
4.442GluThr: 4.442 ± 0.061
4.453GluVal: 4.453 ± 0.077
0.826GluTrp: 0.826 ± 0.03
2.141GluTyr: 2.141 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
3.053PheAla: 3.053 ± 0.071
0.308PheCys: 0.308 ± 0.018
2.507PheAsp: 2.507 ± 0.047
2.711PheGlu: 2.711 ± 0.058
2.275PhePhe: 2.275 ± 0.06
3.279PheGly: 3.279 ± 0.066
0.958PheHis: 0.958 ± 0.03
3.701PheIle: 3.701 ± 0.075
2.097PheLys: 2.097 ± 0.044
4.343PheLeu: 4.343 ± 0.083
1.321PheMet: 1.321 ± 0.034
1.989PheAsn: 1.989 ± 0.048
1.663PhePro: 1.663 ± 0.043
1.626PheGln: 1.626 ± 0.043
1.611PheArg: 1.611 ± 0.044
3.14PheSer: 3.14 ± 0.059
2.523PheThr: 2.523 ± 0.054
2.921PheVal: 2.921 ± 0.06
0.54PheTrp: 0.54 ± 0.024
1.695PheTyr: 1.695 ± 0.047
0.0PheXaa: 0.0 ± 0.0
Gly
4.862GlyAla: 4.862 ± 0.083
0.522GlyCys: 0.522 ± 0.023
3.751GlyAsp: 3.751 ± 0.075
4.678GlyGlu: 4.678 ± 0.076
3.442GlyPhe: 3.442 ± 0.063
4.883GlyGly: 4.883 ± 0.089
1.411GlyHis: 1.411 ± 0.042
5.703GlyIle: 5.703 ± 0.086
4.476GlyLys: 4.476 ± 0.074
6.524GlyLeu: 6.524 ± 0.093
2.265GlyMet: 2.265 ± 0.046
2.939GlyAsn: 2.939 ± 0.066
1.81GlyPro: 1.81 ± 0.045
2.151GlyGln: 2.151 ± 0.048
2.618GlyArg: 2.618 ± 0.059
4.194GlySer: 4.194 ± 0.068
4.047GlyThr: 4.047 ± 0.077
5.054GlyVal: 5.054 ± 0.08
0.874GlyTrp: 0.874 ± 0.036
2.823GlyTyr: 2.823 ± 0.065
0.0GlyXaa: 0.0 ± 0.0
His
1.424HisAla: 1.424 ± 0.037
0.193HisCys: 0.193 ± 0.014
1.327HisAsp: 1.327 ± 0.036
1.506HisGlu: 1.506 ± 0.048
1.043HisPhe: 1.043 ± 0.032
1.505HisGly: 1.505 ± 0.04
0.694HisHis: 0.694 ± 0.03
1.611HisIle: 1.611 ± 0.048
1.044HisLys: 1.044 ± 0.033
2.133HisLeu: 2.133 ± 0.053
0.606HisMet: 0.606 ± 0.025
0.934HisAsn: 0.934 ± 0.033
1.127HisPro: 1.127 ± 0.034
0.917HisGln: 0.917 ± 0.032
0.949HisArg: 0.949 ± 0.032
1.101HisSer: 1.101 ± 0.035
1.1HisThr: 1.1 ± 0.035
1.544HisVal: 1.544 ± 0.038
0.221HisTrp: 0.221 ± 0.015
1.022HisTyr: 1.022 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
5.955IleAla: 5.955 ± 0.087
0.566IleCys: 0.566 ± 0.024
4.669IleAsp: 4.669 ± 0.067
5.177IleGlu: 5.177 ± 0.079
3.217IlePhe: 3.217 ± 0.069
6.018IleGly: 6.018 ± 0.101
1.684IleHis: 1.684 ± 0.04
5.802IleIle: 5.802 ± 0.108
3.846IleLys: 3.846 ± 0.075
6.623IleLeu: 6.623 ± 0.101
2.04IleMet: 2.04 ± 0.05
3.496IleAsn: 3.496 ± 0.068
3.286IlePro: 3.286 ± 0.053
2.729IleGln: 2.729 ± 0.054
3.157IleArg: 3.157 ± 0.062
4.875IleSer: 4.875 ± 0.073
4.382IleThr: 4.382 ± 0.071
5.238IleVal: 5.238 ± 0.071
0.679IleTrp: 0.679 ± 0.032
2.348IleTyr: 2.348 ± 0.05
0.0IleXaa: 0.0 ± 0.0
Lys
4.179LysAla: 4.179 ± 0.061
0.292LysCys: 0.292 ± 0.019
3.548LysAsp: 3.548 ± 0.066
5.415LysGlu: 5.415 ± 0.083
1.667LysPhe: 1.667 ± 0.045
3.661LysGly: 3.661 ± 0.071
1.478LysHis: 1.478 ± 0.042
3.822LysIle: 3.822 ± 0.063
4.606LysLys: 4.606 ± 0.085
5.027LysLeu: 5.027 ± 0.099
1.968LysMet: 1.968 ± 0.047
3.038LysAsn: 3.038 ± 0.066
2.149LysPro: 2.149 ± 0.042
3.282LysGln: 3.282 ± 0.078
3.356LysArg: 3.356 ± 0.066
3.223LysSer: 3.223 ± 0.059
3.457LysThr: 3.457 ± 0.066
3.528LysVal: 3.528 ± 0.056
0.757LysTrp: 0.757 ± 0.028
1.956LysTyr: 1.956 ± 0.049
0.0LysXaa: 0.0 ± 0.0
Leu
6.925LeuAla: 6.925 ± 0.096
0.537LeuCys: 0.537 ± 0.023
5.183LeuAsp: 5.183 ± 0.074
6.379LeuGlu: 6.379 ± 0.094
4.652LeuPhe: 4.652 ± 0.091
6.093LeuGly: 6.093 ± 0.086
1.911LeuHis: 1.911 ± 0.048
6.912LeuIle: 6.912 ± 0.097
5.636LeuLys: 5.636 ± 0.091
9.233LeuLeu: 9.233 ± 0.131
2.678LeuMet: 2.678 ± 0.051
4.47LeuAsn: 4.47 ± 0.066
3.813LeuPro: 3.813 ± 0.06
3.42LeuGln: 3.42 ± 0.062
3.486LeuArg: 3.486 ± 0.069
6.263LeuSer: 6.263 ± 0.093
5.64LeuThr: 5.64 ± 0.08
5.695LeuVal: 5.695 ± 0.091
0.819LeuTrp: 0.819 ± 0.03
3.077LeuTyr: 3.077 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
2.364MetAla: 2.364 ± 0.049
0.147MetCys: 0.147 ± 0.013
1.799MetAsp: 1.799 ± 0.046
2.193MetGlu: 2.193 ± 0.051
1.162MetPhe: 1.162 ± 0.036
1.945MetGly: 1.945 ± 0.043
0.567MetHis: 0.567 ± 0.023
2.249MetIle: 2.249 ± 0.055
2.196MetLys: 2.196 ± 0.054
2.655MetLeu: 2.655 ± 0.057
0.931MetMet: 0.931 ± 0.031
1.644MetAsn: 1.644 ± 0.042
1.152MetPro: 1.152 ± 0.038
1.122MetGln: 1.122 ± 0.033
1.197MetArg: 1.197 ± 0.035
1.773MetSer: 1.773 ± 0.048
1.936MetThr: 1.936 ± 0.044
1.845MetVal: 1.845 ± 0.042
0.234MetTrp: 0.234 ± 0.015
0.801MetTyr: 0.801 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
3.054AsnAla: 3.054 ± 0.057
0.298AsnCys: 0.298 ± 0.018
2.856AsnAsp: 2.856 ± 0.063
3.786AsnGlu: 3.786 ± 0.066
1.626AsnPhe: 1.626 ± 0.043
3.412AsnGly: 3.412 ± 0.069
1.18AsnHis: 1.18 ± 0.035
3.395AsnIle: 3.395 ± 0.069
2.688AsnLys: 2.688 ± 0.066
3.86AsnLeu: 3.86 ± 0.061
1.472AsnMet: 1.472 ± 0.038
2.343AsnAsn: 2.343 ± 0.066
2.186AsnPro: 2.186 ± 0.052
2.283AsnGln: 2.283 ± 0.056
2.217AsnArg: 2.217 ± 0.041
2.342AsnSer: 2.342 ± 0.052
2.485AsnThr: 2.485 ± 0.051
3.17AsnVal: 3.17 ± 0.049
0.614AsnTrp: 0.614 ± 0.026
1.596AsnTyr: 1.596 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
2.419ProAla: 2.419 ± 0.053
0.18ProCys: 0.18 ± 0.014
2.7ProAsp: 2.7 ± 0.049
3.474ProGlu: 3.474 ± 0.073
1.932ProPhe: 1.932 ± 0.046
2.467ProGly: 2.467 ± 0.051
0.814ProHis: 0.814 ± 0.033
2.611ProIle: 2.611 ± 0.049
1.894ProLys: 1.894 ± 0.051
3.295ProLeu: 3.295 ± 0.055
0.86ProMet: 0.86 ± 0.032
1.538ProAsn: 1.538 ± 0.042
1.113ProPro: 1.113 ± 0.036
1.06ProGln: 1.06 ± 0.031
1.1ProArg: 1.1 ± 0.031
2.172ProSer: 2.172 ± 0.045
1.788ProThr: 1.788 ± 0.048
3.13ProVal: 3.13 ± 0.061
0.379ProTrp: 0.379 ± 0.02
1.463ProTyr: 1.463 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
2.895GlnAla: 2.895 ± 0.064
0.191GlnCys: 0.191 ± 0.015
2.149GlnAsp: 2.149 ± 0.053
3.341GlnGlu: 3.341 ± 0.063
1.544GlnPhe: 1.544 ± 0.041
2.226GlnGly: 2.226 ± 0.049
0.887GlnHis: 0.887 ± 0.028
2.522GlnIle: 2.522 ± 0.049
2.599GlnLys: 2.599 ± 0.052
4.065GlnLeu: 4.065 ± 0.075
1.233GlnMet: 1.233 ± 0.038
1.93GlnAsn: 1.93 ± 0.048
1.317GlnPro: 1.317 ± 0.04
2.125GlnGln: 2.125 ± 0.06
1.563GlnArg: 1.563 ± 0.037
2.313GlnSer: 2.313 ± 0.049
2.346GlnThr: 2.346 ± 0.05
2.342GlnVal: 2.342 ± 0.051
0.428GlnTrp: 0.428 ± 0.022
1.297GlnTyr: 1.297 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.398ArgAla: 2.398 ± 0.049
0.269ArgCys: 0.269 ± 0.017
2.435ArgAsp: 2.435 ± 0.052
3.438ArgGlu: 3.438 ± 0.073
1.938ArgPhe: 1.938 ± 0.045
2.383ArgGly: 2.383 ± 0.053
1.023ArgHis: 1.023 ± 0.037
3.147ArgIle: 3.147 ± 0.063
3.186ArgLys: 3.186 ± 0.065
3.913ArgLeu: 3.913 ± 0.064
1.368ArgMet: 1.368 ± 0.041
2.049ArgAsn: 2.049 ± 0.048
1.291ArgPro: 1.291 ± 0.036
1.899ArgGln: 1.899 ± 0.05
1.914ArgArg: 1.914 ± 0.052
2.268ArgSer: 2.268 ± 0.052
2.137ArgThr: 2.137 ± 0.052
2.519ArgVal: 2.519 ± 0.05
0.409ArgTrp: 0.409 ± 0.018
1.592ArgTyr: 1.592 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
3.868SerAla: 3.868 ± 0.072
0.351SerCys: 0.351 ± 0.02
3.66SerAsp: 3.66 ± 0.063
4.185SerGlu: 4.185 ± 0.074
3.007SerPhe: 3.007 ± 0.064
4.782SerGly: 4.782 ± 0.083
1.32SerHis: 1.32 ± 0.031
4.788SerIle: 4.788 ± 0.066
3.077SerLys: 3.077 ± 0.07
5.638SerLeu: 5.638 ± 0.077
1.753SerMet: 1.753 ± 0.044
2.468SerAsn: 2.468 ± 0.06
2.12SerPro: 2.12 ± 0.051
2.12SerGln: 2.12 ± 0.047
2.507SerArg: 2.507 ± 0.058
3.739SerSer: 3.739 ± 0.077
2.938SerThr: 2.938 ± 0.057
4.213SerVal: 4.213 ± 0.061
0.641SerTrp: 0.641 ± 0.026
2.173SerTyr: 2.173 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
4.334ThrAla: 4.334 ± 0.077
0.349ThrCys: 0.349 ± 0.021
3.427ThrAsp: 3.427 ± 0.059
3.923ThrGlu: 3.923 ± 0.07
2.645ThrPhe: 2.645 ± 0.051
4.492ThrGly: 4.492 ± 0.083
1.096ThrHis: 1.096 ± 0.035
4.591ThrIle: 4.591 ± 0.072
2.87ThrLys: 2.87 ± 0.058
4.996ThrLeu: 4.996 ± 0.071
1.458ThrMet: 1.458 ± 0.036
2.507ThrAsn: 2.507 ± 0.05
2.242ThrPro: 2.242 ± 0.043
1.579ThrGln: 1.579 ± 0.042
1.959ThrArg: 1.959 ± 0.04
3.253ThrSer: 3.253 ± 0.061
2.991ThrThr: 2.991 ± 0.064
4.152ThrVal: 4.152 ± 0.067
0.592ThrTrp: 0.592 ± 0.028
2.013ThrTyr: 2.013 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
4.589ValAla: 4.589 ± 0.075
0.532ValCys: 0.532 ± 0.024
3.896ValAsp: 3.896 ± 0.072
4.61ValGlu: 4.61 ± 0.079
3.035ValPhe: 3.035 ± 0.064
4.207ValGly: 4.207 ± 0.074
1.36ValHis: 1.36 ± 0.041
5.738ValIle: 5.738 ± 0.083
3.982ValLys: 3.982 ± 0.074
6.643ValLeu: 6.643 ± 0.085
2.11ValMet: 2.11 ± 0.05
3.221ValAsn: 3.221 ± 0.054
2.599ValPro: 2.599 ± 0.052
2.341ValGln: 2.341 ± 0.049
2.585ValArg: 2.585 ± 0.051
4.438ValSer: 4.438 ± 0.066
4.287ValThr: 4.287 ± 0.073
4.812ValVal: 4.812 ± 0.079
0.628ValTrp: 0.628 ± 0.026
2.287ValTyr: 2.287 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.752TrpAla: 0.752 ± 0.034
0.072TrpCys: 0.072 ± 0.009
0.611TrpAsp: 0.611 ± 0.027
0.725TrpGlu: 0.725 ± 0.029
0.521TrpPhe: 0.521 ± 0.02
0.641TrpGly: 0.641 ± 0.024
0.252TrpHis: 0.252 ± 0.015
0.81TrpIle: 0.81 ± 0.035
0.647TrpLys: 0.647 ± 0.03
1.149TrpLeu: 1.149 ± 0.034
0.359TrpMet: 0.359 ± 0.018
0.53TrpAsn: 0.53 ± 0.024
0.264TrpPro: 0.264 ± 0.016
0.447TrpGln: 0.447 ± 0.024
0.468TrpArg: 0.468 ± 0.023
0.562TrpSer: 0.562 ± 0.026
0.545TrpThr: 0.545 ± 0.025
0.716TrpVal: 0.716 ± 0.024
0.135TrpTrp: 0.135 ± 0.014
0.36TrpTyr: 0.36 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.205TyrAla: 2.205 ± 0.049
0.241TyrCys: 0.241 ± 0.017
2.266TyrAsp: 2.266 ± 0.049
2.473TyrGlu: 2.473 ± 0.053
1.823TyrPhe: 1.823 ± 0.043
2.56TyrGly: 2.56 ± 0.056
0.904TyrHis: 0.904 ± 0.032
2.493TyrIle: 2.493 ± 0.05
1.833TyrLys: 1.833 ± 0.049
3.385TyrLeu: 3.385 ± 0.065
0.89TyrMet: 0.89 ± 0.031
1.534TyrAsn: 1.534 ± 0.037
1.448TyrPro: 1.448 ± 0.039
1.679TyrGln: 1.679 ± 0.049
1.664TyrArg: 1.664 ± 0.045
1.953TyrSer: 1.953 ± 0.051
1.891TyrThr: 1.891 ± 0.041
2.257TyrVal: 2.257 ± 0.046
0.4TyrTrp: 0.4 ± 0.022
1.456TyrTyr: 1.456 ± 0.049
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3575 proteins (970708 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski