Neisseria meningitidis serogroup B (strain MC58)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae; Neisseria; Neisseria meningitidis; Neisseria meningitidis serogroup B

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2001 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9JYA5|Q9JYA5_NEIMB ABC_trans_aux domain-containing protein OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=NMB1672 PE=4 SV=1
MM1 pKa = 7.85EE2 pKa = 5.46EE3 pKa = 4.46LLNDD7 pKa = 3.92GSIRR11 pKa = 11.84LHH13 pKa = 7.42DD14 pKa = 3.92YY15 pKa = 10.33TDD17 pKa = 4.87GIGIPLTGTSKK28 pKa = 10.78EE29 pKa = 4.03QVQKK33 pKa = 11.21LKK35 pKa = 10.78DD36 pKa = 3.22IWPTLEE42 pKa = 4.29DD43 pKa = 4.03AQAIWPEE50 pKa = 4.07DD51 pKa = 2.93PWYY54 pKa = 10.24YY55 pKa = 11.09LEE57 pKa = 4.14WLWWDD62 pKa = 3.77IACPIDD68 pKa = 5.64LADD71 pKa = 5.09LPNIDD76 pKa = 4.75IYY78 pKa = 11.09EE79 pKa = 4.13QAA81 pKa = 3.96

Molecular weight: 9.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P95377|LPXD_NEIMB UDP-3-O-acylglucosamine N-acyltransferase OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=lpxD PE=3 SV=2
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVTKK11 pKa = 10.56RR12 pKa = 11.84KK13 pKa = 7.91RR14 pKa = 11.84THH16 pKa = 5.89GFLVRR21 pKa = 11.84SKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2001

0

2001

583208

22

2703

291.5

32.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.142 ± 0.077

1.053 ± 0.021

5.267 ± 0.043

6.157 ± 0.066

4.104 ± 0.044

7.766 ± 0.062

2.204 ± 0.025

5.859 ± 0.039

5.64 ± 0.055

9.863 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.456 ± 0.03

4.117 ± 0.059

4.184 ± 0.042

4.005 ± 0.046

5.481 ± 0.054

5.547 ± 0.043

5.226 ± 0.045

6.784 ± 0.051

1.177 ± 0.023

2.968 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski