Mycobacterium virus Mozy
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1D4J8|G1D4J8_9CAUD Uncharacterized protein OS=Mycobacterium virus Mozy OX=1034112 GN=84 PE=4 SV=1
MM1 pKa = 7.11 THH3 pKa = 6.14 QPDD6 pKa = 3.97 EE7 pKa = 4.49 IEE9 pKa = 5.4 PISQMEE15 pKa = 4.34 LDD17 pKa = 3.82 ILATSGDD24 pKa = 4.09 LEE26 pKa = 4.66 LATQAGDD33 pKa = 3.35 VARR36 pKa = 11.84 WAFTQAVEE44 pKa = 4.38 SLCTLLCLPPEE55 pKa = 4.38 VAEE58 pKa = 4.0 QMVNEE63 pKa = 4.35 WFLPP67 pKa = 3.8
Molecular weight: 7.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.867
IPC2_protein 3.706
IPC_protein 3.528
Toseland 3.389
ProMoST 3.719
Dawson 3.516
Bjellqvist 3.694
Wikipedia 3.452
Rodwell 3.389
Grimsley 3.312
Solomon 3.465
Lehninger 3.414
Nozaki 3.681
DTASelect 3.745
Thurlkill 3.452
EMBOSS 3.465
Sillero 3.656
Patrickios 1.774
IPC_peptide 3.465
IPC2_peptide 3.63
IPC2.peptide.svr19 3.691
Protein with the highest isoelectric point:
>tr|G1D4K7|G1D4K7_9CAUD Uncharacterized protein OS=Mycobacterium virus Mozy OX=1034112 GN=93 PE=4 SV=1
MM1 pKa = 7.71 SDD3 pKa = 3.43 AEE5 pKa = 4.21 TARR8 pKa = 11.84 RR9 pKa = 11.84 NGWTVGTRR17 pKa = 11.84 LAGDD21 pKa = 3.71 EE22 pKa = 4.26 EE23 pKa = 4.96 RR24 pKa = 11.84 GEE26 pKa = 4.52 TTWIPIPIAPGYY38 pKa = 9.05 EE39 pKa = 3.66 ISSDD43 pKa = 3.24 GSRR46 pKa = 11.84 VRR48 pKa = 11.84 SVDD51 pKa = 2.57 RR52 pKa = 11.84 WVQTAIGRR60 pKa = 11.84 QFWRR64 pKa = 11.84 GKK66 pKa = 8.69 DD67 pKa = 3.65 LKK69 pKa = 10.43 IQPSFNGRR77 pKa = 11.84 YY78 pKa = 8.6 LQVNVRR84 pKa = 11.84 LEE86 pKa = 4.27 GEE88 pKa = 4.63 SIRR91 pKa = 11.84 KK92 pKa = 7.65 PVSVHH97 pKa = 6.3 RR98 pKa = 11.84 LACIAFHH105 pKa = 6.7 GPPPSPDD112 pKa = 2.91 HH113 pKa = 6.69 LVRR116 pKa = 11.84 HH117 pKa = 6.12 LNDD120 pKa = 2.77 VGTDD124 pKa = 3.47 NRR126 pKa = 11.84 PDD128 pKa = 3.4 NLRR131 pKa = 11.84 WGTKK135 pKa = 9.3 SEE137 pKa = 3.91 NAYY140 pKa = 10.83 DD141 pKa = 4.1 SIRR144 pKa = 11.84 LGTHH148 pKa = 6.3 FLSKK152 pKa = 10.41 QKK154 pKa = 9.79 QCKK157 pKa = 9.29 RR158 pKa = 11.84 GHH160 pKa = 5.97 PLIRR164 pKa = 11.84 MKK166 pKa = 10.94 SSDD169 pKa = 4.02 HH170 pKa = 6.19 RR171 pKa = 11.84 WCPQCHH177 pKa = 5.57 RR178 pKa = 11.84 EE179 pKa = 4.51 GYY181 pKa = 9.39 HH182 pKa = 6.12 RR183 pKa = 11.84 RR184 pKa = 11.84 AQARR188 pKa = 11.84 RR189 pKa = 11.84 ALQPPKK195 pKa = 10.35 PPITHH200 pKa = 6.9 CPQGHH205 pKa = 6.82 EE206 pKa = 4.05 YY207 pKa = 10.04 TPEE210 pKa = 3.44 NTYY213 pKa = 10.37 TYY215 pKa = 8.04 THH217 pKa = 6.79 SYY219 pKa = 10.12 KK220 pKa = 10.62 EE221 pKa = 4.19 GRR223 pKa = 11.84 TKK225 pKa = 9.7 TARR228 pKa = 11.84 TCKK231 pKa = 7.68 TCHH234 pKa = 6.71 RR235 pKa = 11.84 EE236 pKa = 3.73 RR237 pKa = 11.84 EE238 pKa = 4.04 KK239 pKa = 10.88 EE240 pKa = 3.47 RR241 pKa = 11.84 RR242 pKa = 11.84 ARR244 pKa = 11.84 KK245 pKa = 8.66 RR246 pKa = 11.84 QSRR249 pKa = 3.63
Molecular weight: 28.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.487
IPC_protein 10.014
Toseland 10.379
ProMoST 10.131
Dawson 10.526
Bjellqvist 10.233
Wikipedia 10.716
Rodwell 10.76
Grimsley 10.584
Solomon 10.584
Lehninger 10.555
Nozaki 10.409
DTASelect 10.204
Thurlkill 10.394
EMBOSS 10.774
Sillero 10.452
Patrickios 10.423
IPC_peptide 10.584
IPC2_peptide 9.326
IPC2.peptide.svr19 8.558
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
108
0
108
18362
30
1176
170.0
18.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.478 ± 0.422
1.296 ± 0.181
6.421 ± 0.219
5.925 ± 0.319
2.925 ± 0.203
8.981 ± 0.572
2.304 ± 0.205
4.384 ± 0.199
3.262 ± 0.206
7.118 ± 0.231
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.282 ± 0.122
3.377 ± 0.146
5.958 ± 0.193
3.562 ± 0.213
6.753 ± 0.387
6.149 ± 0.292
6.65 ± 0.24
7.216 ± 0.255
2.309 ± 0.157
2.652 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here