Simian immunodeficiency virus - agm
Average proteome isoelectric point is 7.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4WRK8|A0A1B4WRK8_SIV Vpr protein OS=Simian immunodeficiency virus - agm OX=11726 GN=vpr PE=4 SV=1
MM1 pKa = 7.77 ASGGSEE7 pKa = 3.64 NRR9 pKa = 11.84 EE10 pKa = 3.73 RR11 pKa = 11.84 RR12 pKa = 11.84 PGWLEE17 pKa = 3.13 IWDD20 pKa = 4.36 LSRR23 pKa = 11.84 EE24 pKa = 4.13 PWDD27 pKa = 3.12 EE28 pKa = 3.74 WLRR31 pKa = 11.84 DD32 pKa = 3.59 MLTDD36 pKa = 4.33 LNQEE40 pKa = 3.87 ARR42 pKa = 11.84 RR43 pKa = 11.84 HH44 pKa = 5.41 FGRR47 pKa = 11.84 EE48 pKa = 3.53 LLFQVWNYY56 pKa = 9.32 CQEE59 pKa = 3.83 EE60 pKa = 4.96 GEE62 pKa = 4.26 RR63 pKa = 11.84 NEE65 pKa = 4.0 VPMSEE70 pKa = 3.81 RR71 pKa = 11.84 AYY73 pKa = 9.28 RR74 pKa = 11.84 YY75 pKa = 9.88 YY76 pKa = 10.85 RR77 pKa = 11.84 IVQKK81 pKa = 11.07 ALFVHH86 pKa = 6.57 FRR88 pKa = 11.84 CGCRR92 pKa = 11.84 RR93 pKa = 11.84 RR94 pKa = 11.84 TPFEE98 pKa = 4.09 PYY100 pKa = 9.24 EE101 pKa = 3.85 EE102 pKa = 4.16 RR103 pKa = 11.84 RR104 pKa = 11.84 NGEE107 pKa = 3.68 GGGRR111 pKa = 11.84 AGRR114 pKa = 11.84 VPPGLEE120 pKa = 3.91 DD121 pKa = 3.15
Molecular weight: 14.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.86
IPC2_protein 5.97
IPC_protein 5.995
Toseland 6.135
ProMoST 5.842
Dawson 6.186
Bjellqvist 6.186
Wikipedia 6.16
Rodwell 6.16
Grimsley 6.186
Solomon 6.186
Lehninger 6.173
Nozaki 6.402
DTASelect 6.561
Thurlkill 6.561
EMBOSS 6.532
Sillero 6.517
Patrickios 4.393
IPC_peptide 6.211
IPC2_peptide 6.532
IPC2.peptide.svr19 6.519
Protein with the highest isoelectric point:
>tr|A0A1B4WRK1|A0A1B4WRK1_SIV Gag polyprotein OS=Simian immunodeficiency virus - agm OX=11726 GN=gag PE=4 SV=1
MM1 pKa = 7.68 EE2 pKa = 5.77 KK3 pKa = 11.01 GEE5 pKa = 4.44 DD6 pKa = 3.73 EE7 pKa = 4.44 QGGFPLGWKK16 pKa = 8.46 TDD18 pKa = 3.51 NLQPCNNKK26 pKa = 9.44 CFCKK30 pKa = 10.1 RR31 pKa = 11.84 CCYY34 pKa = 10.12 HH35 pKa = 6.61 CQLCFLQKK43 pKa = 10.99 GLGINYY49 pKa = 9.26 APRR52 pKa = 11.84 PRR54 pKa = 11.84 RR55 pKa = 11.84 KK56 pKa = 9.7 KK57 pKa = 10.27 VILPYY62 pKa = 9.5 LHH64 pKa = 7.04 RR65 pKa = 11.84 SISTRR70 pKa = 11.84 GGNSQTTQEE79 pKa = 4.19 EE80 pKa = 4.88 KK81 pKa = 8.4 TTVEE85 pKa = 3.77 RR86 pKa = 11.84 DD87 pKa = 3.26 SRR89 pKa = 11.84 PNQASGRR96 pKa = 11.84 KK97 pKa = 8.33 NLEE100 pKa = 3.61 HH101 pKa = 6.81 KK102 pKa = 9.45 TRR104 pKa = 11.84 GSIGARR110 pKa = 11.84 DD111 pKa = 3.32
Molecular weight: 12.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.139
IPC2_protein 8.756
IPC_protein 8.712
Toseland 9.458
ProMoST 9.224
Dawson 9.706
Bjellqvist 9.56
Wikipedia 9.823
Rodwell 10.043
Grimsley 9.706
Solomon 9.78
Lehninger 9.765
Nozaki 9.853
DTASelect 9.428
Thurlkill 9.619
EMBOSS 9.897
Sillero 9.765
Patrickios 7.571
IPC_peptide 9.78
IPC2_peptide 8.653
IPC2.peptide.svr19 7.803
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
3183
86
1019
397.9
45.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.938 ± 0.356
2.451 ± 0.495
3.739 ± 0.252
7.697 ± 0.797
2.796 ± 0.291
7.886 ± 0.46
2.168 ± 0.314
5.781 ± 0.818
7.289 ± 0.731
8.545 ± 0.379
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.885 ± 0.428
4.555 ± 0.925
5.247 ± 0.539
6.378 ± 0.443
6.283 ± 1.112
4.178 ± 0.242
5.152 ± 0.675
5.592 ± 0.473
3.079 ± 0.246
3.362 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here