Tolypocladium cylindrosporum virus 1
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7BBP7|E7BBP7_9VIRU RNA-directed RNA polymerase OS=Tolypocladium cylindrosporum virus 1 OX=939923 GN=RdRp PE=3 SV=1
MM1 pKa = 7.86 DD2 pKa = 5.02 FIIRR6 pKa = 11.84 NSFLSGVIASPRR18 pKa = 11.84 GGSLDD23 pKa = 3.1 NDD25 pKa = 3.12 NRR27 pKa = 11.84 FRR29 pKa = 11.84 RR30 pKa = 11.84 YY31 pKa = 7.37 RR32 pKa = 11.84 TSVRR36 pKa = 11.84 TTATIGGNDD45 pKa = 3.86 DD46 pKa = 3.68 SRR48 pKa = 11.84 TTSIFYY54 pKa = 10.38 EE55 pKa = 4.05 VGRR58 pKa = 11.84 AVNTKK63 pKa = 9.25 GRR65 pKa = 11.84 ALNRR69 pKa = 11.84 PSDD72 pKa = 3.56 GALCIEE78 pKa = 4.64 AAYY81 pKa = 7.13 PTNTVLAEE89 pKa = 4.09 DD90 pKa = 5.26 FIGLAKK96 pKa = 10.54 KK97 pKa = 8.47 YY98 pKa = 8.69 TNFSASFEE106 pKa = 4.23 YY107 pKa = 10.81 SSLAGVAEE115 pKa = 4.06 RR116 pKa = 11.84 LARR119 pKa = 11.84 ALAASSVFTDD129 pKa = 3.16 VDD131 pKa = 4.2 SNDD134 pKa = 2.92 IRR136 pKa = 11.84 GGAGLVVNAVGTYY149 pKa = 9.9 DD150 pKa = 4.05 GPISSLTNTVYY161 pKa = 10.38 IPRR164 pKa = 11.84 LVNSSITGEE173 pKa = 4.26 VFSVLANAVSGEE185 pKa = 4.15 GSSVATDD192 pKa = 4.25 IIEE195 pKa = 4.41 LDD197 pKa = 3.31 AGTRR201 pKa = 11.84 QPLIPEE207 pKa = 4.07 VDD209 pKa = 3.63 PAGLAAACVDD219 pKa = 3.5 ALRR222 pKa = 11.84 IVGSNMIASDD232 pKa = 3.62 QGPLFSLAVTRR243 pKa = 11.84 GIHH246 pKa = 5.82 RR247 pKa = 11.84 VLSVVGHH254 pKa = 5.45 TDD256 pKa = 2.62 EE257 pKa = 5.93 GGITRR262 pKa = 11.84 DD263 pKa = 3.64 LLRR266 pKa = 11.84 CSSFAPPFGGIHH278 pKa = 6.0 YY279 pKa = 10.37 ALEE282 pKa = 4.83 PYY284 pKa = 10.56 AGLPALQFTAFPAYY298 pKa = 9.82 AAYY301 pKa = 10.32 VDD303 pKa = 5.23 SIALTTAAVVAHH315 pKa = 7.19 ADD317 pKa = 3.45 PGVMYY322 pKa = 10.42 GGSWFPTFYY331 pKa = 11.02 NGTADD336 pKa = 2.9 SDD338 pKa = 4.01 GTVRR342 pKa = 11.84 PGQNQAGTAAMANRR356 pKa = 11.84 NRR358 pKa = 11.84 AQLLGSAPAFFSEE371 pKa = 4.6 YY372 pKa = 10.01 IRR374 pKa = 11.84 GLATIFGASGDD385 pKa = 3.63 SSLACRR391 pKa = 11.84 FMGAASYY398 pKa = 10.82 ALPQDD403 pKa = 3.91 PRR405 pKa = 11.84 HH406 pKa = 5.75 LRR408 pKa = 11.84 YY409 pKa = 8.93 ATVSPWFWIEE419 pKa = 3.69 PTGLLPHH426 pKa = 7.31 DD427 pKa = 4.03 FLGSKK432 pKa = 10.63 AEE434 pKa = 4.11 ANGAGSFAWKK444 pKa = 8.74 DD445 pKa = 3.4 TTRR448 pKa = 11.84 TRR450 pKa = 11.84 VAWDD454 pKa = 3.53 EE455 pKa = 4.01 LTQHH459 pKa = 6.61 GEE461 pKa = 3.64 ADD463 pKa = 3.95 TTFSAYY469 pKa = 8.54 VARR472 pKa = 11.84 FRR474 pKa = 11.84 SPRR477 pKa = 11.84 QQWFFAHH484 pKa = 6.75 WMNHH488 pKa = 4.98 PLNGLGAIRR497 pKa = 11.84 VRR499 pKa = 11.84 QLDD502 pKa = 3.58 PNGIVQPGQCVAHH515 pKa = 7.03 PDD517 pKa = 3.13 VRR519 pKa = 11.84 DD520 pKa = 3.42 RR521 pKa = 11.84 VEE523 pKa = 4.61 ADD525 pKa = 4.14 LPFTDD530 pKa = 4.54 YY531 pKa = 11.29 LWTRR535 pKa = 11.84 GQSPPPAPGEE545 pKa = 3.87 LLNLSGSVGFLVRR558 pKa = 11.84 HH559 pKa = 5.7 LTYY562 pKa = 11.09 DD563 pKa = 3.76 DD564 pKa = 5.25 DD565 pKa = 4.58 GVPHH569 pKa = 6.99 EE570 pKa = 4.37 EE571 pKa = 4.0 HH572 pKa = 6.37 VPTRR576 pKa = 11.84 RR577 pKa = 11.84 EE578 pKa = 3.94 FLDD581 pKa = 3.35 TTVTIEE587 pKa = 3.5 VGRR590 pKa = 11.84 PVGIATGASNAGDD603 pKa = 3.29 NHH605 pKa = 5.37 VRR607 pKa = 11.84 RR608 pKa = 11.84 ARR610 pKa = 11.84 TKK612 pKa = 10.34 AANEE616 pKa = 3.42 LAAARR621 pKa = 11.84 RR622 pKa = 11.84 RR623 pKa = 11.84 AAVFGRR629 pKa = 11.84 ADD631 pKa = 3.47 VAEE634 pKa = 4.15 MPILTSAPALAPPPSAGLEE653 pKa = 3.97 RR654 pKa = 11.84 GSDD657 pKa = 3.54 PGGSGSQRR665 pKa = 11.84 RR666 pKa = 11.84 AVPAGLPGNDD676 pKa = 2.51 TWARR680 pKa = 11.84 EE681 pKa = 4.11 PTGAPRR687 pKa = 11.84 NPVPHH692 pKa = 6.4 HH693 pKa = 5.9 QPLRR697 pKa = 11.84 APQLARR703 pKa = 11.84 QAGGLGGGAAPIPPPPPGVPAPPALPPADD732 pKa = 5.29 DD733 pKa = 5.48 DD734 pKa = 4.3 NAPPAPVATAPPVADD749 pKa = 3.92 PQAMVGDD756 pKa = 4.23 QII758 pKa = 4.63
Molecular weight: 79.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.717
IPC2_protein 5.588
IPC_protein 5.652
Toseland 6.084
ProMoST 5.995
Dawson 5.893
Bjellqvist 5.919
Wikipedia 5.906
Rodwell 5.881
Grimsley 6.275
Solomon 5.893
Lehninger 5.893
Nozaki 6.16
DTASelect 6.364
Thurlkill 6.351
EMBOSS 6.313
Sillero 6.249
Patrickios 4.266
IPC_peptide 5.919
IPC2_peptide 6.275
IPC2.peptide.svr19 6.167
Protein with the highest isoelectric point:
>tr|E7BBP7|E7BBP7_9VIRU RNA-directed RNA polymerase OS=Tolypocladium cylindrosporum virus 1 OX=939923 GN=RdRp PE=3 SV=1
MM1 pKa = 7.13 VFVSGRR7 pKa = 11.84 VGEE10 pKa = 4.65 FGALGSYY17 pKa = 10.26 LHH19 pKa = 7.35 DD20 pKa = 5.03 LLPEE24 pKa = 4.86 LGDD27 pKa = 3.62 VTEE30 pKa = 5.08 SYY32 pKa = 11.86 ADD34 pKa = 3.84 LEE36 pKa = 4.35 LTEE39 pKa = 4.14 QLIKK43 pKa = 10.5 FATHH47 pKa = 5.6 SVSLRR52 pKa = 11.84 GVHH55 pKa = 6.25 PLGPVAVSLLCVAYY69 pKa = 9.08 PVQCDD74 pKa = 3.76 FRR76 pKa = 11.84 LSDD79 pKa = 3.54 LTAVLRR85 pKa = 11.84 HH86 pKa = 5.78 SFSFLPQRR94 pKa = 11.84 APLLEE99 pKa = 3.99 LTGLIEE105 pKa = 4.77 RR106 pKa = 11.84 RR107 pKa = 11.84 VTLRR111 pKa = 11.84 KK112 pKa = 9.91 APATIGALHH121 pKa = 6.17 TKK123 pKa = 8.48 VARR126 pKa = 11.84 LCNSDD131 pKa = 3.11 SAARR135 pKa = 11.84 SQLWPPKK142 pKa = 8.94 RR143 pKa = 11.84 HH144 pKa = 5.7 AAAGTKK150 pKa = 10.25 VNIRR154 pKa = 11.84 LAPLAVALSRR164 pKa = 11.84 SYY166 pKa = 11.34 GPAWLGRR173 pKa = 11.84 ALTPLVGCAEE183 pKa = 4.38 DD184 pKa = 4.45 AVCCALLLATALEE197 pKa = 4.21 PHH199 pKa = 6.84 FGHH202 pKa = 7.42 LGLEE206 pKa = 4.79 VAQSMILEE214 pKa = 4.35 PNNAKK219 pKa = 10.3 GLSNALKK226 pKa = 10.41 ALGWNSTLPGSMLVEE241 pKa = 4.83 GGALQGRR248 pKa = 11.84 GVSPVDD254 pKa = 3.32 MDD256 pKa = 4.52 AEE258 pKa = 4.38 IASRR262 pKa = 11.84 TTPDD266 pKa = 3.62 LVTDD270 pKa = 3.93 MVISSAAEE278 pKa = 3.71 MAPHH282 pKa = 6.39 IRR284 pKa = 11.84 AILALEE290 pKa = 4.45 LPSNSGLGDD299 pKa = 3.8 LDD301 pKa = 4.28 EE302 pKa = 4.9 FWSARR307 pKa = 11.84 WLWCVNGAQNRR318 pKa = 11.84 ASDD321 pKa = 3.61 RR322 pKa = 11.84 SLNLPSPVRR331 pKa = 11.84 GAKK334 pKa = 9.93 RR335 pKa = 11.84 YY336 pKa = 9.53 RR337 pKa = 11.84 RR338 pKa = 11.84 MAAEE342 pKa = 4.27 EE343 pKa = 4.26 VSSNPIYY350 pKa = 10.97 DD351 pKa = 3.31 WDD353 pKa = 3.64 GTTNVSASTKK363 pKa = 10.34 LEE365 pKa = 4.31 PGKK368 pKa = 10.73 SRR370 pKa = 11.84 AIFACDD376 pKa = 3.03 TSSYY380 pKa = 10.84 FAFSWILDD388 pKa = 3.42 RR389 pKa = 11.84 AQRR392 pKa = 11.84 DD393 pKa = 3.59 WRR395 pKa = 11.84 GHH397 pKa = 5.46 RR398 pKa = 11.84 VLLNPGEE405 pKa = 4.34 GGLYY409 pKa = 10.07 GVARR413 pKa = 11.84 RR414 pKa = 11.84 VKK416 pKa = 9.26 GAQGRR421 pKa = 11.84 GGVNVMLDD429 pKa = 3.4 YY430 pKa = 11.58 DD431 pKa = 4.74 DD432 pKa = 5.89 FNSHH436 pKa = 6.63 HH437 pKa = 6.74 SLGVQQEE444 pKa = 4.29 LTRR447 pKa = 11.84 QLCQLYY453 pKa = 9.8 NAPSWYY459 pKa = 9.58 TSVLVDD465 pKa = 3.8 SFDD468 pKa = 3.58 RR469 pKa = 11.84 MYY471 pKa = 9.63 ITHH474 pKa = 6.89 RR475 pKa = 11.84 GSRR478 pKa = 11.84 KK479 pKa = 9.8 RR480 pKa = 11.84 ILGTLMSGHH489 pKa = 7.14 RR490 pKa = 11.84 GTSFINSVLNAAYY503 pKa = 9.38 IRR505 pKa = 11.84 AAVGGAFFDD514 pKa = 3.99 RR515 pKa = 11.84 LVSLHH520 pKa = 6.86 AGDD523 pKa = 4.51 DD524 pKa = 3.41 AYY526 pKa = 10.81 MRR528 pKa = 11.84 CSTLAEE534 pKa = 4.16 AAHH537 pKa = 5.82 VLTRR541 pKa = 11.84 CARR544 pKa = 11.84 FGCRR548 pKa = 11.84 MNPTKK553 pKa = 10.53 QSIGFRR559 pKa = 11.84 HH560 pKa = 6.48 AEE562 pKa = 3.93 FLRR565 pKa = 11.84 LGIGDD570 pKa = 4.47 KK571 pKa = 11.0 YY572 pKa = 11.29 AVGYY576 pKa = 9.11 LCRR579 pKa = 11.84 SISTLVAGSWVAPDD593 pKa = 4.15 PLSPEE598 pKa = 4.88 DD599 pKa = 4.28 GLTSAITTVRR609 pKa = 11.84 SCINRR614 pKa = 11.84 GCPSSLSRR622 pKa = 11.84 IIARR626 pKa = 11.84 TYY628 pKa = 11.11 SSIHH632 pKa = 6.34 GYY634 pKa = 7.9 PLRR637 pKa = 11.84 VLDD640 pKa = 4.69 ALLSGAACLEE650 pKa = 4.13 SGAVYY655 pKa = 8.76 NTDD658 pKa = 3.01 YY659 pKa = 10.89 TLRR662 pKa = 11.84 QYY664 pKa = 10.98 RR665 pKa = 11.84 VVRR668 pKa = 11.84 PLPDD672 pKa = 4.84 DD673 pKa = 4.61 LPLPPQHH680 pKa = 6.92 RR681 pKa = 11.84 EE682 pKa = 3.68 YY683 pKa = 10.67 ATRR686 pKa = 11.84 EE687 pKa = 3.69 YY688 pKa = 10.59 LANHH692 pKa = 6.09 VSPIEE697 pKa = 3.87 ARR699 pKa = 11.84 AAQLASADD707 pKa = 3.86 LVRR710 pKa = 11.84 IMVRR714 pKa = 11.84 SSYY717 pKa = 10.95 SKK719 pKa = 10.28 GVTRR723 pKa = 11.84 HH724 pKa = 5.92 PSSPTSRR731 pKa = 11.84 RR732 pKa = 11.84 RR733 pKa = 11.84 PQLVSLPTVKK743 pKa = 10.03 ATGFTTAAEE752 pKa = 4.07 LAKK755 pKa = 10.26 RR756 pKa = 11.84 QPPVGKK762 pKa = 9.69 LANFPLLRR770 pKa = 11.84 LIEE773 pKa = 4.24 ARR775 pKa = 11.84 LTDD778 pKa = 3.86 DD779 pKa = 4.67 QISEE783 pKa = 4.35 LLSFNGTPPGGLPPRR798 pKa = 11.84 IAAFGVEE805 pKa = 4.12 GHH807 pKa = 6.53 CCNVIGYY814 pKa = 9.1 LPYY817 pKa = 10.71 SDD819 pKa = 3.98 ACSYY823 pKa = 11.09 SKK825 pKa = 10.13 RR826 pKa = 11.84 TTCDD830 pKa = 3.09 NIIAGYY836 pKa = 9.3 SVYY839 pKa = 11.07 SS840 pKa = 3.59
Molecular weight: 91.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.197
IPC2_protein 8.229
IPC_protein 8.053
Toseland 7.702
ProMoST 8.595
Dawson 8.741
Bjellqvist 9.077
Wikipedia 8.712
Rodwell 8.77
Grimsley 7.732
Solomon 8.799
Lehninger 8.785
Nozaki 9.194
DTASelect 8.799
Thurlkill 8.829
EMBOSS 8.916
Sillero 9.136
Patrickios 4.291
IPC_peptide 8.785
IPC2_peptide 8.331
IPC2.peptide.svr19 8.352
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1598
758
840
799.0
85.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.203 ± 1.088
1.564 ± 0.597
5.319 ± 0.494
3.63 ± 0.132
3.379 ± 0.469
8.824 ± 0.706
2.378 ± 0.176
3.88 ± 0.051
1.564 ± 0.51
9.825 ± 1.607
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.314 ± 0.171
3.442 ± 0.253
7.259 ± 1.042
2.503 ± 0.089
7.822 ± 0.286
8.073 ± 0.8
5.882 ± 0.384
6.884 ± 0.072
1.252 ± 0.042
3.004 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here