Cotton leafroll dwarf virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 7.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E2DRQ7|E2DRQ7_9LUTE Movement protein OS=Cotton leafroll dwarf virus OX=312295 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.72EE2 pKa = 5.73EE3 pKa = 4.68DD4 pKa = 3.6DD5 pKa = 4.74HH6 pKa = 9.43VGATGVGKK14 pKa = 10.4ISQWLWSKK22 pKa = 10.74PLGTHH27 pKa = 6.0NAEE30 pKa = 4.22DD31 pKa = 4.52DD32 pKa = 3.68EE33 pKa = 5.71DD34 pKa = 4.61EE35 pKa = 4.29EE36 pKa = 4.85VVIGQEE42 pKa = 4.18DD43 pKa = 4.19AFLEE47 pKa = 4.34DD48 pKa = 3.31QEE50 pKa = 4.39LQARR54 pKa = 11.84HH55 pKa = 6.09LFSQKK60 pKa = 8.26TVSRR64 pKa = 11.84EE65 pKa = 3.85VPQDD69 pKa = 3.13QSRR72 pKa = 11.84SGRR75 pKa = 11.84LYY77 pKa = 10.43QIARR81 pKa = 11.84HH82 pKa = 5.4SAMEE86 pKa = 3.86YY87 pKa = 10.48SRR89 pKa = 11.84PTMNIRR95 pKa = 11.84SQWSYY100 pKa = 8.96WSSSPRR106 pKa = 11.84PLQHH110 pKa = 6.81PPVPSLTKK118 pKa = 9.91WIHH121 pKa = 5.11TVNCQPYY128 pKa = 9.26PPRR131 pKa = 11.84LTNSEE136 pKa = 4.31SPRR139 pKa = 11.84MGGSNLRR146 pKa = 11.84RR147 pKa = 11.84LSSMDD152 pKa = 3.63RR153 pKa = 11.84NGMTPPRR160 pKa = 11.84TNSEE164 pKa = 4.61SYY166 pKa = 9.16TKK168 pKa = 11.27AMVPRR173 pKa = 11.84RR174 pKa = 3.73

Molecular weight: 19.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E2DRQ7|E2DRQ7_9LUTE Movement protein OS=Cotton leafroll dwarf virus OX=312295 GN=ORF4 PE=3 SV=1
MM1 pKa = 6.49NTVVGRR7 pKa = 11.84RR8 pKa = 11.84TINGRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84PRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84NRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84QNQPVVVVQAPRR35 pKa = 11.84NTQRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84GGRR48 pKa = 11.84NRR50 pKa = 11.84TGGRR54 pKa = 11.84IPGGPGASSEE64 pKa = 4.36TFVFSKK70 pKa = 10.85DD71 pKa = 3.53SLSGSSSGSITFGPSLSDD89 pKa = 4.1CPAFSNGILKK99 pKa = 10.26AYY101 pKa = 10.04HH102 pKa = 6.58EE103 pKa = 4.7YY104 pKa = 10.05KK105 pKa = 10.29ISMVLLEE112 pKa = 5.28FISEE116 pKa = 4.21ASSTSSGSISYY127 pKa = 10.2EE128 pKa = 3.66VDD130 pKa = 3.18PHH132 pKa = 6.84CKK134 pKa = 10.27LSTLSSTINKK144 pKa = 9.78FGITKK149 pKa = 10.4NGRR152 pKa = 11.84KK153 pKa = 8.85QFAASFINGQEE164 pKa = 3.71WHH166 pKa = 7.11DD167 pKa = 3.61TSEE170 pKa = 3.96DD171 pKa = 3.38QFRR174 pKa = 11.84ILYY177 pKa = 9.14KK178 pKa = 11.0GNGSSSIAGSFKK190 pKa = 10.22VTIRR194 pKa = 11.84CQFHH198 pKa = 6.06NPKK201 pKa = 10.51

Molecular weight: 22.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3046

174

1072

507.7

56.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.026 ± 0.815

1.51 ± 0.212

4.169 ± 0.729

5.778 ± 0.242

4.728 ± 0.52

7.157 ± 0.398

1.97 ± 0.169

3.611 ± 0.377

4.957 ± 0.571

8.798 ± 1.405

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.576 ± 0.234

4.137 ± 0.335

6.796 ± 0.56

3.71 ± 0.357

6.829 ± 1.038

10.867 ± 0.754

5.548 ± 0.434

6.074 ± 0.466

2.035 ± 0.253

2.692 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski