Dragonfly-associated circular virus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemyduguivirus; Gemyduguivirus draga1; Dragonfly associated gemyduguivirus 1

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0A2I7|K0A2I7_9VIRU Replication-associated protein OS=Dragonfly-associated circular virus 3 OX=1234884 PE=3 SV=1
MM1 pKa = 7.82DD2 pKa = 4.3QLSFDD7 pKa = 4.07TDD9 pKa = 3.62DD10 pKa = 3.18VNYY13 pKa = 11.01AVFDD17 pKa = 5.38DD18 pKa = 3.52IHH20 pKa = 7.3SLKK23 pKa = 10.09FFPMWKK29 pKa = 9.42FWMGAQDD36 pKa = 3.75TFTVTDD42 pKa = 3.79KK43 pKa = 11.55YY44 pKa = 10.25KK45 pKa = 11.42GKK47 pKa = 8.14MTFNWGRR54 pKa = 11.84PIIWCNNKK62 pKa = 9.92DD63 pKa = 3.48PRR65 pKa = 11.84ADD67 pKa = 3.71PDD69 pKa = 5.01ADD71 pKa = 4.6ADD73 pKa = 4.32WIDD76 pKa = 3.75ANCIVVNVPEE86 pKa = 4.4DD87 pKa = 3.61TPLISRR93 pKa = 11.84ANTEE97 pKa = 3.79

Molecular weight: 11.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0A1L3|K0A1L3_9VIRU Replication-associated protein OS=Dragonfly-associated circular virus 3 OX=1234884 PE=4 SV=1
MM1 pKa = 7.82AYY3 pKa = 10.41ASRR6 pKa = 11.84RR7 pKa = 11.84YY8 pKa = 9.55ARR10 pKa = 11.84RR11 pKa = 11.84PRR13 pKa = 11.84RR14 pKa = 11.84SLRR17 pKa = 11.84TSRR20 pKa = 11.84YY21 pKa = 8.32RR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84GPRR28 pKa = 11.84RR29 pKa = 11.84VRR31 pKa = 11.84RR32 pKa = 11.84TTFRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GTSKK42 pKa = 10.4RR43 pKa = 11.84RR44 pKa = 11.84ILNVSSKK51 pKa = 10.54KK52 pKa = 10.35KK53 pKa = 9.01RR54 pKa = 11.84DD55 pKa = 3.28NMINVSFSPTGINPAVRR72 pKa = 11.84GFIATGAAPTMLVWCATARR91 pKa = 11.84DD92 pKa = 3.89RR93 pKa = 11.84VSNASNPTADD103 pKa = 4.09SVRR106 pKa = 11.84EE107 pKa = 4.02SQTCYY112 pKa = 9.5MRR114 pKa = 11.84GLKK117 pKa = 9.09EE118 pKa = 4.09HH119 pKa = 6.62IRR121 pKa = 11.84INTNNPTSWLWRR133 pKa = 11.84RR134 pKa = 11.84ICFTAKK140 pKa = 10.49GLFSGLGTSADD151 pKa = 3.53SVEE154 pKa = 4.54TSNGWVRR161 pKa = 11.84ALMDD165 pKa = 3.22QTGTSFGTSLTNLLFAGTNGVDD187 pKa = 3.16WNDD190 pKa = 3.12TFTANTDD197 pKa = 3.43STNVKK202 pKa = 9.8IMYY205 pKa = 9.22DD206 pKa = 3.08KK207 pKa = 11.05TFTINSGVSGVIRR220 pKa = 11.84DD221 pKa = 3.55YY222 pKa = 11.89KK223 pKa = 9.47MWHH226 pKa = 5.98GMNKK230 pKa = 9.93NIVYY234 pKa = 10.63GEE236 pKa = 4.25DD237 pKa = 3.4EE238 pKa = 4.27NGEE241 pKa = 4.13GEE243 pKa = 4.09INNRR247 pKa = 11.84YY248 pKa = 7.85STIGRR253 pKa = 11.84PGMGDD258 pKa = 3.69YY259 pKa = 11.13YY260 pKa = 11.47VVDD263 pKa = 4.67LLASNSSAAADD274 pKa = 3.44TLTFSTEE281 pKa = 3.42ATLYY285 pKa = 8.61WHH287 pKa = 7.09EE288 pKa = 4.26KK289 pKa = 9.3

Molecular weight: 32.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

965

97

323

241.3

27.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.254 ± 0.206

1.554 ± 0.192

7.565 ± 1.618

4.56 ± 0.778

5.699 ± 0.814

7.979 ± 0.805

1.451 ± 0.199

4.456 ± 0.294

4.145 ± 0.594

5.803 ± 0.567

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.798 ± 0.266

5.389 ± 0.994

4.974 ± 0.849

2.28 ± 0.651

7.668 ± 1.446

7.15 ± 0.918

7.358 ± 1.409

5.285 ± 0.227

2.798 ± 0.457

3.834 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski