Streptomyces armeniacus
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6639 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345XV89|A0A345XV89_9ACTN Aldo/keto reductase OS=Streptomyces armeniacus OX=83291 GN=DVA86_25835 PE=4 SV=1
MM1 pKa = 7.14 TVHH4 pKa = 7.2 DD5 pKa = 4.47 VAAVLPGIPVLRR17 pKa = 11.84 DD18 pKa = 3.39 LCRR21 pKa = 11.84 SMAMADD27 pKa = 3.33 AVLNPDD33 pKa = 3.93 DD34 pKa = 3.85 EE35 pKa = 5.2 PYY37 pKa = 11.28 YY38 pKa = 10.84 SFNAGWSEE46 pKa = 4.14 TEE48 pKa = 4.01 EE49 pKa = 4.16 LASMRR54 pKa = 11.84 TGSGDD59 pKa = 3.12 EE60 pKa = 3.83 FDD62 pKa = 4.71 IVFSSAGAYY71 pKa = 8.96 IRR73 pKa = 11.84 GFDD76 pKa = 3.99 HH77 pKa = 7.51 EE78 pKa = 5.31 SPLSPYY84 pKa = 10.33 HH85 pKa = 6.95 RR86 pKa = 11.84 DD87 pKa = 3.63 DD88 pKa = 5.46 VPAPWPGVVDD98 pKa = 3.62 TVPAVFRR105 pKa = 11.84 GLVSEE110 pKa = 4.53 PAFTDD115 pKa = 3.29 EE116 pKa = 5.63 DD117 pKa = 3.98 GTPVVTVCMWRR128 pKa = 11.84 QEE130 pKa = 4.44 GDD132 pKa = 3.83 DD133 pKa = 3.46 RR134 pKa = 11.84 WQAGQIDD141 pKa = 4.48 FPGGHH146 pKa = 7.71 PDD148 pKa = 3.58 PDD150 pKa = 4.08 GSDD153 pKa = 2.77 WLFGLLADD161 pKa = 4.47 PTPEE165 pKa = 3.82 AFQAFAEE172 pKa = 4.8 DD173 pKa = 4.5 YY174 pKa = 10.89 YY175 pKa = 10.41 EE176 pKa = 3.98 TAVDD180 pKa = 3.8 IEE182 pKa = 4.59 AVRR185 pKa = 11.84 HH186 pKa = 4.88 VYY188 pKa = 10.19 GLRR191 pKa = 11.84 PLDD194 pKa = 3.75 HH195 pKa = 6.69 SAVAGLNPDD204 pKa = 3.18 ASYY207 pKa = 11.81 DD208 pKa = 3.85 DD209 pKa = 4.53 LVAAAEE215 pKa = 4.59 AIGYY219 pKa = 8.04 PLAA222 pKa = 5.8
Molecular weight: 24.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.846
IPC_protein 3.859
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.986
Patrickios 1.914
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.883
Protein with the highest isoelectric point:
>tr|A0A345XYC7|A0A345XYC7_9ACTN Uncharacterized protein OS=Streptomyces armeniacus OX=83291 GN=DVA86_32825 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIVANRR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.91 GRR40 pKa = 11.84 SKK42 pKa = 11.16 LSAA45 pKa = 3.74
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6639
0
6639
2263626
18
3830
341.0
36.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.934 ± 0.045
0.803 ± 0.008
6.105 ± 0.022
6.025 ± 0.029
2.634 ± 0.019
9.916 ± 0.03
2.331 ± 0.014
2.786 ± 0.02
1.953 ± 0.025
10.25 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.7 ± 0.012
1.639 ± 0.014
6.266 ± 0.029
2.679 ± 0.018
8.373 ± 0.033
4.966 ± 0.018
5.936 ± 0.023
8.197 ± 0.027
1.464 ± 0.012
2.043 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here