Bacillus phage BSP38

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Jeonjuvirus; Bacillus virus BSP38

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 254 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MJR9|A0A345MJR9_9CAUD Uncharacterized protein OS=Bacillus phage BSP38 OX=2283013 GN=BSP38_059 PE=4 SV=1
MM1 pKa = 7.42FGLSDD6 pKa = 4.16KK7 pKa = 11.04EE8 pKa = 4.03LIAYY12 pKa = 8.47RR13 pKa = 11.84NTIHH17 pKa = 8.06DD18 pKa = 4.24YY19 pKa = 11.36VLDD22 pKa = 4.47DD23 pKa = 4.4LSPYY27 pKa = 10.16IIEE30 pKa = 4.32KK31 pKa = 8.77VVYY34 pKa = 7.45MAPFITKK41 pKa = 10.24DD42 pKa = 3.43GEE44 pKa = 4.31RR45 pKa = 11.84TPDD48 pKa = 3.45LQFVSTLVRR57 pKa = 11.84DD58 pKa = 3.89VEE60 pKa = 4.34NVILLAISAMSYY72 pKa = 10.48EE73 pKa = 4.38YY74 pKa = 11.44AHH76 pKa = 6.26LQCDD80 pKa = 3.57LDD82 pKa = 4.79LNEE85 pKa = 4.52KK86 pKa = 10.61DD87 pKa = 3.17IIEE90 pKa = 4.36RR91 pKa = 11.84LHH93 pKa = 5.77YY94 pKa = 10.5KK95 pKa = 9.44YY96 pKa = 11.2DD97 pKa = 3.26NFIVHH102 pKa = 6.94KK103 pKa = 9.56FIQKK107 pKa = 10.36GIAFSPEE114 pKa = 3.29IVIEE118 pKa = 4.34IIGDD122 pKa = 4.5AILQLPYY129 pKa = 10.09IYY131 pKa = 11.09VNAIEE136 pKa = 5.91DD137 pKa = 3.56EE138 pKa = 4.7DD139 pKa = 4.5FDD141 pKa = 4.25EE142 pKa = 6.69DD143 pKa = 4.17SFLEE147 pKa = 4.17EE148 pKa = 4.1RR149 pKa = 11.84ADD151 pKa = 3.64AYY153 pKa = 10.76SAYY156 pKa = 9.46IDD158 pKa = 3.63EE159 pKa = 4.9NFTEE163 pKa = 4.32EE164 pKa = 4.42EE165 pKa = 3.83EE166 pKa = 5.49DD167 pKa = 5.0DD168 pKa = 4.33FGDD171 pKa = 3.92YY172 pKa = 11.09

Molecular weight: 20.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MK15|A0A345MK15_9CAUD Uncharacterized protein OS=Bacillus phage BSP38 OX=2283013 GN=BSP38_155 PE=4 SV=1
MM1 pKa = 7.54AKK3 pKa = 9.9KK4 pKa = 10.22SKK6 pKa = 9.65PNSVSRR12 pKa = 11.84SMSRR16 pKa = 11.84GAGARR21 pKa = 11.84GTSAIPNSGRR31 pKa = 11.84EE32 pKa = 4.16AYY34 pKa = 7.97TKK36 pKa = 10.43SRR38 pKa = 11.84QSTRR42 pKa = 11.84GHH44 pKa = 5.16YY45 pKa = 9.94RR46 pKa = 11.84IEE48 pKa = 3.86FTQVYY53 pKa = 10.43EE54 pKa = 4.16NMTEE58 pKa = 3.96KK59 pKa = 10.63DD60 pKa = 3.32VEE62 pKa = 4.01LRR64 pKa = 11.84KK65 pKa = 10.4VYY67 pKa = 10.42GVDD70 pKa = 3.87LVAGNLGVPIDD81 pKa = 4.52MITLKK86 pKa = 10.67AKK88 pKa = 10.46KK89 pKa = 8.67KK90 pKa = 7.85TAQTLTSLDD99 pKa = 3.18EE100 pKa = 4.04VFYY103 pKa = 11.4VKK105 pKa = 10.57VGQDD109 pKa = 3.12TCGKK113 pKa = 10.35LSIRR117 pKa = 11.84TQRR120 pKa = 11.84LWKK123 pKa = 10.27GSILIFVFQKK133 pKa = 10.42KK134 pKa = 7.28KK135 pKa = 8.81TAIKK139 pKa = 9.56PPQKK143 pKa = 10.47AFSRR147 pKa = 11.84NKK149 pKa = 9.84GYY151 pKa = 10.95KK152 pKa = 9.55KK153 pKa = 9.43RR154 pKa = 11.84TASQKK159 pKa = 10.05SQASRR164 pKa = 11.84NSYY167 pKa = 9.16NKK169 pKa = 9.69RR170 pKa = 11.84RR171 pKa = 11.84GGTRR175 pKa = 2.92

Molecular weight: 19.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

254

0

254

45917

35

1275

180.8

20.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.359 ± 0.194

0.769 ± 0.066

6.601 ± 0.175

8.352 ± 0.277

3.818 ± 0.108

6.309 ± 0.242

1.76 ± 0.101

6.231 ± 0.155

7.514 ± 0.206

8.439 ± 0.189

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.084

5.066 ± 0.147

3.225 ± 0.126

3.652 ± 0.149

4.482 ± 0.126

6.248 ± 0.203

6.139 ± 0.207

6.895 ± 0.136

1.03 ± 0.061

4.414 ± 0.108
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski