Amino acid dipepetide frequency for Streptococcus phage CHPC676

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.67AlaAla: 5.67 ± 1.625
0.247AlaCys: 0.247 ± 0.17
4.273AlaAsp: 4.273 ± 0.533
4.191AlaGlu: 4.191 ± 0.654
2.629AlaPhe: 2.629 ± 0.991
5.67AlaGly: 5.67 ± 0.986
0.822AlaHis: 0.822 ± 0.262
6.327AlaIle: 6.327 ± 1.572
5.834AlaLys: 5.834 ± 0.73
6.984AlaLeu: 6.984 ± 1.233
2.465AlaMet: 2.465 ± 0.922
3.78AlaAsn: 3.78 ± 0.659
2.301AlaPro: 2.301 ± 0.451
2.629AlaGln: 2.629 ± 0.904
3.122AlaArg: 3.122 ± 0.559
6.163AlaSer: 6.163 ± 1.514
4.601AlaThr: 4.601 ± 0.749
3.615AlaVal: 3.615 ± 0.918
1.15AlaTrp: 1.15 ± 0.466
2.383AlaTyr: 2.383 ± 0.563
0.0AlaXaa: 0.0 ± 0.0
Cys
0.164CysAla: 0.164 ± 0.153
0.0CysCys: 0.0 ± 0.0
0.657CysAsp: 0.657 ± 0.29
0.411CysGlu: 0.411 ± 0.18
0.082CysPhe: 0.082 ± 0.101
0.74CysGly: 0.74 ± 0.347
0.164CysHis: 0.164 ± 0.131
0.164CysIle: 0.164 ± 0.116
0.493CysLys: 0.493 ± 0.256
0.411CysLeu: 0.411 ± 0.233
0.082CysMet: 0.082 ± 0.079
0.329CysAsn: 0.329 ± 0.178
0.082CysPro: 0.082 ± 0.085
0.082CysGln: 0.082 ± 0.085
0.247CysArg: 0.247 ± 0.17
0.575CysSer: 0.575 ± 0.333
0.0CysThr: 0.0 ± 0.0
0.164CysVal: 0.164 ± 0.109
0.082CysTrp: 0.082 ± 0.081
0.575CysTyr: 0.575 ± 0.34
0.0CysXaa: 0.0 ± 0.0
Asp
3.205AspAla: 3.205 ± 0.498
0.74AspCys: 0.74 ± 0.273
4.601AspAsp: 4.601 ± 0.616
3.369AspGlu: 3.369 ± 0.622
3.369AspPhe: 3.369 ± 0.538
6.409AspGly: 6.409 ± 1.69
0.575AspHis: 0.575 ± 0.265
3.698AspIle: 3.698 ± 0.637
5.177AspLys: 5.177 ± 0.797
4.355AspLeu: 4.355 ± 0.556
1.643AspMet: 1.643 ± 0.335
4.519AspAsn: 4.519 ± 0.741
0.986AspPro: 0.986 ± 0.358
1.726AspGln: 1.726 ± 0.347
2.958AspArg: 2.958 ± 0.46
4.273AspSer: 4.273 ± 0.711
3.369AspThr: 3.369 ± 0.557
3.205AspVal: 3.205 ± 0.513
1.315AspTrp: 1.315 ± 0.402
3.287AspTyr: 3.287 ± 0.594
0.0AspXaa: 0.0 ± 0.0
Glu
5.341GluAla: 5.341 ± 0.849
0.329GluCys: 0.329 ± 0.186
2.465GluAsp: 2.465 ± 0.45
3.287GluGlu: 3.287 ± 0.489
2.794GluPhe: 2.794 ± 0.456
3.451GluGly: 3.451 ± 0.468
1.15GluHis: 1.15 ± 0.292
5.259GluIle: 5.259 ± 0.845
4.437GluLys: 4.437 ± 0.956
6.491GluLeu: 6.491 ± 1.076
1.89GluMet: 1.89 ± 0.319
4.355GluAsn: 4.355 ± 0.64
1.808GluPro: 1.808 ± 0.537
2.301GluGln: 2.301 ± 0.666
3.287GluArg: 3.287 ± 0.636
2.712GluSer: 2.712 ± 0.484
3.862GluThr: 3.862 ± 0.668
4.766GluVal: 4.766 ± 0.76
1.068GluTrp: 1.068 ± 0.27
3.533GluTyr: 3.533 ± 0.721
0.0GluXaa: 0.0 ± 0.0
Phe
2.301PheAla: 2.301 ± 0.33
0.411PheCys: 0.411 ± 0.239
2.958PheAsp: 2.958 ± 0.63
3.205PheGlu: 3.205 ± 0.629
1.233PhePhe: 1.233 ± 0.312
3.369PheGly: 3.369 ± 0.698
0.411PheHis: 0.411 ± 0.202
2.547PheIle: 2.547 ± 0.521
4.93PheLys: 4.93 ± 0.627
1.89PheLeu: 1.89 ± 0.487
0.904PheMet: 0.904 ± 0.329
3.04PheAsn: 3.04 ± 0.481
0.575PhePro: 0.575 ± 0.245
1.233PheGln: 1.233 ± 0.308
1.561PheArg: 1.561 ± 0.367
2.547PheSer: 2.547 ± 0.434
2.629PheThr: 2.629 ± 0.655
1.808PheVal: 1.808 ± 0.398
0.657PheTrp: 0.657 ± 0.205
1.068PheTyr: 1.068 ± 0.349
0.0PheXaa: 0.0 ± 0.0
Gly
5.094GlyAla: 5.094 ± 0.723
0.082GlyCys: 0.082 ± 0.084
3.533GlyAsp: 3.533 ± 0.59
3.944GlyGlu: 3.944 ± 0.513
2.629GlyPhe: 2.629 ± 0.52
3.451GlyGly: 3.451 ± 0.515
0.74GlyHis: 0.74 ± 0.223
6.081GlyIle: 6.081 ± 1.589
7.806GlyLys: 7.806 ± 1.19
6.574GlyLeu: 6.574 ± 0.652
1.808GlyMet: 1.808 ± 0.652
3.369GlyAsn: 3.369 ± 0.487
0.822GlyPro: 0.822 ± 0.303
3.04GlyGln: 3.04 ± 0.527
3.451GlyArg: 3.451 ± 0.78
4.519GlySer: 4.519 ± 0.652
5.998GlyThr: 5.998 ± 0.833
4.437GlyVal: 4.437 ± 0.736
0.904GlyTrp: 0.904 ± 0.246
3.04GlyTyr: 3.04 ± 0.514
0.0GlyXaa: 0.0 ± 0.0
His
0.575HisAla: 0.575 ± 0.249
0.082HisCys: 0.082 ± 0.098
1.15HisAsp: 1.15 ± 0.316
0.822HisGlu: 0.822 ± 0.278
0.411HisPhe: 0.411 ± 0.191
0.904HisGly: 0.904 ± 0.345
0.493HisHis: 0.493 ± 0.203
0.822HisIle: 0.822 ± 0.218
0.74HisLys: 0.74 ± 0.31
1.15HisLeu: 1.15 ± 0.286
0.329HisMet: 0.329 ± 0.18
0.493HisAsn: 0.493 ± 0.158
0.575HisPro: 0.575 ± 0.213
0.164HisGln: 0.164 ± 0.137
0.822HisArg: 0.822 ± 0.283
0.986HisSer: 0.986 ± 0.314
0.986HisThr: 0.986 ± 0.197
0.904HisVal: 0.904 ± 0.27
0.082HisTrp: 0.082 ± 0.08
0.575HisTyr: 0.575 ± 0.192
0.0HisXaa: 0.0 ± 0.0
Ile
5.67IleAla: 5.67 ± 1.168
0.493IleCys: 0.493 ± 0.206
5.177IleAsp: 5.177 ± 0.625
4.519IleGlu: 4.519 ± 0.716
1.972IlePhe: 1.972 ± 0.393
4.766IleGly: 4.766 ± 0.964
0.74IleHis: 0.74 ± 0.193
3.451IleIle: 3.451 ± 0.578
6.163IleLys: 6.163 ± 0.588
3.369IleLeu: 3.369 ± 0.486
1.89IleMet: 1.89 ± 0.438
3.78IleAsn: 3.78 ± 0.676
2.794IlePro: 2.794 ± 0.523
2.958IleGln: 2.958 ± 0.452
2.712IleArg: 2.712 ± 0.51
5.998IleSer: 5.998 ± 1.366
4.108IleThr: 4.108 ± 0.733
3.451IleVal: 3.451 ± 0.857
0.575IleTrp: 0.575 ± 0.223
3.533IleTyr: 3.533 ± 0.672
0.0IleXaa: 0.0 ± 0.0
Lys
7.067LysAla: 7.067 ± 0.703
0.329LysCys: 0.329 ± 0.186
4.766LysAsp: 4.766 ± 0.836
7.642LysGlu: 7.642 ± 1.159
2.629LysPhe: 2.629 ± 0.59
5.916LysGly: 5.916 ± 0.973
0.822LysHis: 0.822 ± 0.285
4.191LysIle: 4.191 ± 0.73
5.998LysLys: 5.998 ± 1.145
6.574LysLeu: 6.574 ± 0.756
1.972LysMet: 1.972 ± 0.633
4.519LysAsn: 4.519 ± 0.78
2.712LysPro: 2.712 ± 0.482
2.301LysGln: 2.301 ± 0.468
4.684LysArg: 4.684 ± 0.804
4.766LysSer: 4.766 ± 0.775
5.341LysThr: 5.341 ± 0.685
4.273LysVal: 4.273 ± 0.627
1.233LysTrp: 1.233 ± 0.274
3.944LysTyr: 3.944 ± 1.093
0.0LysXaa: 0.0 ± 0.0
Leu
6.245LeuAla: 6.245 ± 0.979
0.247LeuCys: 0.247 ± 0.163
5.094LeuAsp: 5.094 ± 0.601
6.409LeuGlu: 6.409 ± 1.067
3.04LeuPhe: 3.04 ± 0.45
5.423LeuGly: 5.423 ± 0.926
0.657LeuHis: 0.657 ± 0.244
4.026LeuIle: 4.026 ± 0.6
6.327LeuLys: 6.327 ± 0.672
4.191LeuLeu: 4.191 ± 0.591
1.643LeuMet: 1.643 ± 0.339
5.423LeuAsn: 5.423 ± 0.592
2.794LeuPro: 2.794 ± 0.476
2.794LeuGln: 2.794 ± 0.438
2.794LeuArg: 2.794 ± 0.713
5.094LeuSer: 5.094 ± 0.718
6.491LeuThr: 6.491 ± 0.887
4.93LeuVal: 4.93 ± 0.591
0.986LeuTrp: 0.986 ± 0.414
2.301LeuTyr: 2.301 ± 0.449
0.0LeuXaa: 0.0 ± 0.0
Met
2.712MetAla: 2.712 ± 0.858
0.082MetCys: 0.082 ± 0.085
1.15MetAsp: 1.15 ± 0.333
1.397MetGlu: 1.397 ± 0.372
0.904MetPhe: 0.904 ± 0.256
1.315MetGly: 1.315 ± 0.457
0.575MetHis: 0.575 ± 0.226
1.315MetIle: 1.315 ± 0.393
2.629MetLys: 2.629 ± 0.55
1.561MetLeu: 1.561 ± 0.317
1.233MetMet: 1.233 ± 0.565
1.15MetAsn: 1.15 ± 0.283
0.575MetPro: 0.575 ± 0.164
1.561MetGln: 1.561 ± 0.564
1.15MetArg: 1.15 ± 0.292
1.808MetSer: 1.808 ± 0.406
1.397MetThr: 1.397 ± 0.366
1.397MetVal: 1.397 ± 0.416
0.164MetTrp: 0.164 ± 0.112
0.657MetTyr: 0.657 ± 0.322
0.0MetXaa: 0.0 ± 0.0
Asn
3.369AsnAla: 3.369 ± 0.572
0.411AsnCys: 0.411 ± 0.17
4.191AsnAsp: 4.191 ± 0.944
3.862AsnGlu: 3.862 ± 0.62
2.219AsnPhe: 2.219 ± 0.416
5.259AsnGly: 5.259 ± 0.697
1.561AsnHis: 1.561 ± 0.42
3.862AsnIle: 3.862 ± 0.595
3.369AsnLys: 3.369 ± 0.666
4.437AsnLeu: 4.437 ± 0.511
1.068AsnMet: 1.068 ± 0.328
3.04AsnAsn: 3.04 ± 0.651
2.465AsnPro: 2.465 ± 0.468
1.561AsnGln: 1.561 ± 0.426
1.726AsnArg: 1.726 ± 0.4
3.122AsnSer: 3.122 ± 0.384
3.205AsnThr: 3.205 ± 0.599
3.78AsnVal: 3.78 ± 0.476
1.643AsnTrp: 1.643 ± 0.512
1.972AsnTyr: 1.972 ± 0.465
0.0AsnXaa: 0.0 ± 0.0
Pro
1.726ProAla: 1.726 ± 0.447
0.247ProCys: 0.247 ± 0.232
1.972ProAsp: 1.972 ± 0.365
1.643ProGlu: 1.643 ± 0.447
0.986ProPhe: 0.986 ± 0.295
1.808ProGly: 1.808 ± 0.585
0.329ProHis: 0.329 ± 0.18
1.89ProIle: 1.89 ± 0.328
2.547ProLys: 2.547 ± 0.449
2.054ProLeu: 2.054 ± 0.62
0.411ProMet: 0.411 ± 0.189
2.136ProAsn: 2.136 ± 0.631
0.822ProPro: 0.822 ± 0.216
1.068ProGln: 1.068 ± 0.274
0.904ProArg: 0.904 ± 0.309
2.219ProSer: 2.219 ± 0.408
1.89ProThr: 1.89 ± 0.523
2.301ProVal: 2.301 ± 0.506
0.164ProTrp: 0.164 ± 0.119
0.74ProTyr: 0.74 ± 0.251
0.0ProXaa: 0.0 ± 0.0
Gln
3.862GlnAla: 3.862 ± 0.795
0.329GlnCys: 0.329 ± 0.145
1.561GlnAsp: 1.561 ± 0.334
2.794GlnGlu: 2.794 ± 0.587
1.643GlnPhe: 1.643 ± 0.418
2.383GlnGly: 2.383 ± 0.646
0.411GlnHis: 0.411 ± 0.172
2.794GlnIle: 2.794 ± 0.716
2.301GlnLys: 2.301 ± 0.411
3.122GlnLeu: 3.122 ± 0.406
0.74GlnMet: 0.74 ± 0.269
1.726GlnAsn: 1.726 ± 0.288
0.986GlnPro: 0.986 ± 0.337
1.315GlnGln: 1.315 ± 0.342
1.479GlnArg: 1.479 ± 0.292
2.794GlnSer: 2.794 ± 0.655
2.547GlnThr: 2.547 ± 0.393
2.547GlnVal: 2.547 ± 0.37
0.411GlnTrp: 0.411 ± 0.183
1.397GlnTyr: 1.397 ± 0.447
0.0GlnXaa: 0.0 ± 0.0
Arg
2.876ArgAla: 2.876 ± 0.544
0.411ArgCys: 0.411 ± 0.226
2.054ArgAsp: 2.054 ± 0.315
2.547ArgGlu: 2.547 ± 0.584
1.726ArgPhe: 1.726 ± 0.385
2.629ArgGly: 2.629 ± 0.398
0.493ArgHis: 0.493 ± 0.239
3.04ArgIle: 3.04 ± 0.739
3.369ArgLys: 3.369 ± 0.795
3.451ArgLeu: 3.451 ± 0.646
1.561ArgMet: 1.561 ± 0.36
2.136ArgAsn: 2.136 ± 0.472
0.904ArgPro: 0.904 ± 0.233
1.643ArgGln: 1.643 ± 0.331
2.219ArgArg: 2.219 ± 0.492
2.219ArgSer: 2.219 ± 0.422
2.383ArgThr: 2.383 ± 0.603
3.205ArgVal: 3.205 ± 0.554
0.657ArgTrp: 0.657 ± 0.225
2.712ArgTyr: 2.712 ± 0.439
0.0ArgXaa: 0.0 ± 0.0
Ser
5.752SerAla: 5.752 ± 2.7
0.164SerCys: 0.164 ± 0.12
4.437SerAsp: 4.437 ± 0.72
3.862SerGlu: 3.862 ± 0.636
2.794SerPhe: 2.794 ± 0.487
5.259SerGly: 5.259 ± 0.679
0.657SerHis: 0.657 ± 0.264
5.177SerIle: 5.177 ± 0.884
4.437SerLys: 4.437 ± 0.702
5.012SerLeu: 5.012 ± 0.789
1.726SerMet: 1.726 ± 0.343
3.944SerAsn: 3.944 ± 0.564
1.643SerPro: 1.643 ± 0.366
3.698SerGln: 3.698 ± 0.645
2.712SerArg: 2.712 ± 0.492
5.998SerSer: 5.998 ± 1.144
3.615SerThr: 3.615 ± 0.55
5.998SerVal: 5.998 ± 0.856
0.575SerTrp: 0.575 ± 0.221
1.972SerTyr: 1.972 ± 0.438
0.0SerXaa: 0.0 ± 0.0
Thr
4.355ThrAla: 4.355 ± 1.036
0.329ThrCys: 0.329 ± 0.19
3.944ThrAsp: 3.944 ± 0.906
2.876ThrGlu: 2.876 ± 0.493
3.533ThrPhe: 3.533 ± 0.586
4.273ThrGly: 4.273 ± 0.646
1.15ThrHis: 1.15 ± 0.234
5.505ThrIle: 5.505 ± 0.68
6.081ThrLys: 6.081 ± 0.707
5.67ThrLeu: 5.67 ± 0.7
1.068ThrMet: 1.068 ± 0.449
2.054ThrAsn: 2.054 ± 0.382
1.561ThrPro: 1.561 ± 0.348
2.301ThrGln: 2.301 ± 0.442
1.972ThrArg: 1.972 ± 0.406
4.355ThrSer: 4.355 ± 0.863
4.355ThrThr: 4.355 ± 0.613
5.505ThrVal: 5.505 ± 0.718
0.657ThrTrp: 0.657 ± 0.308
3.287ThrTyr: 3.287 ± 0.801
0.0ThrXaa: 0.0 ± 0.0
Val
4.684ValAla: 4.684 ± 0.72
0.247ValCys: 0.247 ± 0.148
4.93ValAsp: 4.93 ± 0.782
4.766ValGlu: 4.766 ± 0.693
2.301ValPhe: 2.301 ± 0.544
4.519ValGly: 4.519 ± 0.609
0.575ValHis: 0.575 ± 0.187
4.848ValIle: 4.848 ± 0.752
5.094ValLys: 5.094 ± 0.621
4.355ValLeu: 4.355 ± 0.585
0.904ValMet: 0.904 ± 0.224
3.698ValAsn: 3.698 ± 0.75
2.136ValPro: 2.136 ± 0.391
2.465ValGln: 2.465 ± 0.455
1.972ValArg: 1.972 ± 0.518
5.341ValSer: 5.341 ± 0.59
4.437ValThr: 4.437 ± 0.564
4.273ValVal: 4.273 ± 0.594
1.068ValTrp: 1.068 ± 0.27
1.726ValTyr: 1.726 ± 0.36
0.0ValXaa: 0.0 ± 0.0
Trp
0.822TrpAla: 0.822 ± 0.305
0.082TrpCys: 0.082 ± 0.076
0.822TrpAsp: 0.822 ± 0.296
1.068TrpGlu: 1.068 ± 0.316
0.575TrpPhe: 0.575 ± 0.238
0.986TrpGly: 0.986 ± 0.277
0.164TrpHis: 0.164 ± 0.097
0.74TrpIle: 0.74 ± 0.241
1.068TrpLys: 1.068 ± 0.185
0.904TrpLeu: 0.904 ± 0.306
0.082TrpMet: 0.082 ± 0.083
0.822TrpAsn: 0.822 ± 0.282
0.247TrpPro: 0.247 ± 0.139
0.493TrpGln: 0.493 ± 0.192
0.657TrpArg: 0.657 ± 0.249
1.397TrpSer: 1.397 ± 0.52
1.15TrpThr: 1.15 ± 0.478
1.15TrpVal: 1.15 ± 0.313
0.164TrpTrp: 0.164 ± 0.109
0.411TrpTyr: 0.411 ± 0.169
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.369TyrAla: 3.369 ± 0.612
0.247TyrCys: 0.247 ± 0.151
3.04TyrAsp: 3.04 ± 0.724
1.972TyrGlu: 1.972 ± 0.39
1.808TyrPhe: 1.808 ± 0.378
2.794TyrGly: 2.794 ± 0.429
0.575TyrHis: 0.575 ± 0.251
2.383TyrIle: 2.383 ± 0.663
2.876TyrLys: 2.876 ± 0.489
4.355TyrLeu: 4.355 ± 0.822
1.233TyrMet: 1.233 ± 0.356
1.808TyrAsn: 1.808 ± 0.417
1.068TyrPro: 1.068 ± 0.352
1.808TyrGln: 1.808 ± 0.382
1.89TyrArg: 1.89 ± 0.599
2.547TyrSer: 2.547 ± 0.512
2.465TyrThr: 2.465 ± 0.566
2.629TyrVal: 2.629 ± 0.603
0.247TyrTrp: 0.247 ± 0.161
2.301TyrTyr: 2.301 ± 0.576
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12171 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski