Chenopodium quinoa mitovirus 1
Average proteome isoelectric point is 9.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U8LM99|A0A2U8LM99_9VIRU RNA dependent RNA polymerase OS=Chenopodium quinoa mitovirus 1 OX=2185087 PE=4 SV=1
MM1 pKa = 7.61 IKK3 pKa = 10.58 NLFLNAEE10 pKa = 4.18 RR11 pKa = 11.84 VPILSWRR18 pKa = 11.84 SIFEE22 pKa = 3.61 GTRR25 pKa = 11.84 RR26 pKa = 11.84 LKK28 pKa = 10.63 GLCLRR33 pKa = 11.84 ASLIVSLRR41 pKa = 11.84 LHH43 pKa = 6.66 KK44 pKa = 9.83 EE45 pKa = 3.5 TGLAAVAFARR55 pKa = 11.84 VVFRR59 pKa = 11.84 LVRR62 pKa = 11.84 KK63 pKa = 10.11 SGLLFTSLYY72 pKa = 10.53 LKK74 pKa = 10.4 QCGVSLQRR82 pKa = 11.84 YY83 pKa = 6.94 YY84 pKa = 11.18 AGSYY88 pKa = 10.07 SKK90 pKa = 10.52 QDD92 pKa = 3.54 SLSVPVSLTRR102 pKa = 11.84 TGLPKK107 pKa = 10.12 IIPVYY112 pKa = 8.98 IRR114 pKa = 11.84 RR115 pKa = 11.84 VIRR118 pKa = 11.84 KK119 pKa = 9.13 NDD121 pKa = 3.12 DD122 pKa = 3.62 RR123 pKa = 11.84 ADD125 pKa = 3.28 KK126 pKa = 10.12 WVRR129 pKa = 11.84 TYY131 pKa = 11.23 LSWFSASRR139 pKa = 11.84 LVEE142 pKa = 3.95 LAPSVTSSTFASIHH156 pKa = 5.85 EE157 pKa = 4.48 PIKK160 pKa = 10.9 DD161 pKa = 3.04 IGSVKK166 pKa = 10.23 EE167 pKa = 3.94 VLSVLKK173 pKa = 10.14 MRR175 pKa = 11.84 GRR177 pKa = 11.84 RR178 pKa = 11.84 LVSTYY183 pKa = 11.05 LPFARR188 pKa = 11.84 SIPVYY193 pKa = 10.24 QGLKK197 pKa = 9.54 WEE199 pKa = 4.61 PTWKK203 pKa = 8.5 STPMTTKK210 pKa = 9.62 FVSRR214 pKa = 11.84 YY215 pKa = 9.38 RR216 pKa = 11.84 SLTPEE221 pKa = 4.11 EE222 pKa = 5.52 LSTLDD227 pKa = 3.52 DD228 pKa = 3.8 TNIFSNLKK236 pKa = 10.16 HH237 pKa = 5.88 EE238 pKa = 4.95 LASFMYY244 pKa = 10.25 NINKK248 pKa = 8.66 IHH250 pKa = 6.55 SLPEE254 pKa = 4.08 GFFSPGCLWPEE265 pKa = 4.02 WIIYY269 pKa = 9.51 PFDD272 pKa = 3.94 FKK274 pKa = 10.83 RR275 pKa = 11.84 TTEE278 pKa = 3.67 IANWSLEE285 pKa = 3.82 WFEE288 pKa = 4.98 RR289 pKa = 11.84 RR290 pKa = 11.84 IGPHH294 pKa = 5.25 MSSIVSAYY302 pKa = 9.74 QIHH305 pKa = 6.86 PPVPLFTGKK314 pKa = 10.1 LAQTLPGAGKK324 pKa = 10.01 RR325 pKa = 11.84 RR326 pKa = 11.84 IFAICNYY333 pKa = 8.29 VKK335 pKa = 10.68 QMLLKK340 pKa = 10.04 PIHH343 pKa = 5.8 SWAMKK348 pKa = 9.19 ILSSIPMDD356 pKa = 3.29 GTFNQVAPLLRR367 pKa = 11.84 LAKK370 pKa = 10.01 IKK372 pKa = 9.4 RR373 pKa = 11.84 THH375 pKa = 6.33 VYY377 pKa = 10.79 SFDD380 pKa = 3.81 LKK382 pKa = 10.88 SATDD386 pKa = 3.7 RR387 pKa = 11.84 WPLPIIYY394 pKa = 8.59 TCVASFFGEE403 pKa = 4.89 TYY405 pKa = 10.54 ASSVVNSTLGLNTFRR420 pKa = 11.84 VDD422 pKa = 3.21 KK423 pKa = 10.3 PIVSRR428 pKa = 11.84 MSEE431 pKa = 3.5 IAFRR435 pKa = 11.84 CGQPLGYY442 pKa = 9.66 YY443 pKa = 10.17 GSWSLFALSHH453 pKa = 6.41 HH454 pKa = 5.92 LVVWLAADD462 pKa = 4.01 LAYY465 pKa = 9.79 PSRR468 pKa = 11.84 EE469 pKa = 4.01 TPFRR473 pKa = 11.84 DD474 pKa = 3.62 YY475 pKa = 11.52 AVLGDD480 pKa = 4.6 DD481 pKa = 5.12 VLIADD486 pKa = 4.03 TNVALEE492 pKa = 4.12 YY493 pKa = 10.65 KK494 pKa = 10.42 SLLSRR499 pKa = 11.84 LGVSISEE506 pKa = 4.31 SKK508 pKa = 10.88 SIISEE513 pKa = 3.85 TGAIEE518 pKa = 3.9 FAKK521 pKa = 10.29 KK522 pKa = 9.85 YY523 pKa = 7.65 WVKK526 pKa = 10.85 GMQVDD531 pKa = 4.51 LSPVSLKK538 pKa = 10.82 SLLGARR544 pKa = 11.84 HH545 pKa = 6.28 TIGLCQIGAKK555 pKa = 9.88 YY556 pKa = 10.76 DD557 pKa = 3.77 LDD559 pKa = 4.3 FNTLLRR565 pKa = 11.84 IGGAGYY571 pKa = 10.07 RR572 pKa = 11.84 VRR574 pKa = 11.84 SRR576 pKa = 11.84 QLSTLNRR583 pKa = 11.84 KK584 pKa = 6.75 WEE586 pKa = 4.38 KK587 pKa = 10.31 IRR589 pKa = 11.84 AVQVKK594 pKa = 8.3 LTGQRR599 pKa = 11.84 AQLPIEE605 pKa = 4.08 FWIGRR610 pKa = 11.84 GCPLDD615 pKa = 4.31 PYY617 pKa = 10.68 LKK619 pKa = 10.65 GKK621 pKa = 10.1 IIVYY625 pKa = 9.45 LRR627 pKa = 11.84 KK628 pKa = 9.56 EE629 pKa = 4.35 FKK631 pKa = 10.32 PKK633 pKa = 9.99 EE634 pKa = 3.93 LRR636 pKa = 11.84 LTPEE640 pKa = 4.0 GLLFDD645 pKa = 4.6 GEE647 pKa = 4.47 VEE649 pKa = 3.88 IAEE652 pKa = 4.04 RR653 pKa = 11.84 TVIHH657 pKa = 6.21 NWMKK661 pKa = 10.61 QWLKK665 pKa = 9.8 WIYY668 pKa = 8.85 WYY670 pKa = 11.05 YY671 pKa = 10.25 SVAMSPDD678 pKa = 3.1 VKK680 pKa = 10.72 LDD682 pKa = 3.32 QFFDD686 pKa = 3.72 APICATSWKK695 pKa = 10.12 RR696 pKa = 11.84 NQIDD700 pKa = 4.07 LNLKK704 pKa = 9.98 RR705 pKa = 11.84 FSALWRR711 pKa = 11.84 CYY713 pKa = 11.03 DD714 pKa = 4.07 MAVGWPKK721 pKa = 10.61 NYY723 pKa = 9.68 PWVLPATTVLKK734 pKa = 10.13 IDD736 pKa = 3.78 EE737 pKa = 4.94 LIKK740 pKa = 11.0 GGFSGTDD747 pKa = 3.42 FLMKK751 pKa = 9.96 PVEE754 pKa = 5.66 LIVQQNKK761 pKa = 7.23 GRR763 pKa = 11.84 KK764 pKa = 8.64 YY765 pKa = 9.81 II766 pKa = 3.87
Molecular weight: 87.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.184
IPC2_protein 9.311
IPC_protein 9.282
Toseland 9.926
ProMoST 9.648
Dawson 10.16
Bjellqvist 9.838
Wikipedia 10.321
Rodwell 10.526
Grimsley 10.233
Solomon 10.175
Lehninger 10.131
Nozaki 9.955
DTASelect 9.823
Thurlkill 9.999
EMBOSS 10.335
Sillero 10.072
Patrickios 9.663
IPC_peptide 10.16
IPC2_peptide 8.536
IPC2.peptide.svr19 8.216
Protein with the highest isoelectric point:
>tr|A0A2U8LM99|A0A2U8LM99_9VIRU RNA dependent RNA polymerase OS=Chenopodium quinoa mitovirus 1 OX=2185087 PE=4 SV=1
MM1 pKa = 7.61 IKK3 pKa = 10.58 NLFLNAEE10 pKa = 4.18 RR11 pKa = 11.84 VPILSWRR18 pKa = 11.84 SIFEE22 pKa = 3.61 GTRR25 pKa = 11.84 RR26 pKa = 11.84 LKK28 pKa = 10.63 GLCLRR33 pKa = 11.84 ASLIVSLRR41 pKa = 11.84 LHH43 pKa = 6.66 KK44 pKa = 9.83 EE45 pKa = 3.5 TGLAAVAFARR55 pKa = 11.84 VVFRR59 pKa = 11.84 LVRR62 pKa = 11.84 KK63 pKa = 10.11 SGLLFTSLYY72 pKa = 10.53 LKK74 pKa = 10.4 QCGVSLQRR82 pKa = 11.84 YY83 pKa = 6.94 YY84 pKa = 11.18 AGSYY88 pKa = 10.07 SKK90 pKa = 10.52 QDD92 pKa = 3.54 SLSVPVSLTRR102 pKa = 11.84 TGLPKK107 pKa = 10.12 IIPVYY112 pKa = 8.98 IRR114 pKa = 11.84 RR115 pKa = 11.84 VIRR118 pKa = 11.84 KK119 pKa = 9.13 NDD121 pKa = 3.12 DD122 pKa = 3.62 RR123 pKa = 11.84 ADD125 pKa = 3.28 KK126 pKa = 10.12 WVRR129 pKa = 11.84 TYY131 pKa = 11.23 LSWFSASRR139 pKa = 11.84 LVEE142 pKa = 3.95 LAPSVTSSTFASIHH156 pKa = 5.85 EE157 pKa = 4.48 PIKK160 pKa = 10.9 DD161 pKa = 3.04 IGSVKK166 pKa = 10.23 EE167 pKa = 3.94 VLSVLKK173 pKa = 10.14 MRR175 pKa = 11.84 GRR177 pKa = 11.84 RR178 pKa = 11.84 LVSTYY183 pKa = 11.05 LPFARR188 pKa = 11.84 SIPVYY193 pKa = 10.24 QGLKK197 pKa = 9.54 WEE199 pKa = 4.61 PTWKK203 pKa = 8.5 STPMTTKK210 pKa = 9.62 FVSRR214 pKa = 11.84 YY215 pKa = 9.38 RR216 pKa = 11.84 SLTPEE221 pKa = 4.11 EE222 pKa = 5.52 LSTLDD227 pKa = 3.52 DD228 pKa = 3.8 TNIFSNLKK236 pKa = 10.16 HH237 pKa = 5.88 EE238 pKa = 4.95 LASFMYY244 pKa = 10.25 NINKK248 pKa = 8.66 IHH250 pKa = 6.55 SLPEE254 pKa = 4.08 GFFSPGCLWPEE265 pKa = 4.02 WIIYY269 pKa = 9.51 PFDD272 pKa = 3.94 FKK274 pKa = 10.83 RR275 pKa = 11.84 TTEE278 pKa = 3.67 IANWSLEE285 pKa = 3.82 WFEE288 pKa = 4.98 RR289 pKa = 11.84 RR290 pKa = 11.84 IGPHH294 pKa = 5.25 MSSIVSAYY302 pKa = 9.74 QIHH305 pKa = 6.86 PPVPLFTGKK314 pKa = 10.1 LAQTLPGAGKK324 pKa = 10.01 RR325 pKa = 11.84 RR326 pKa = 11.84 IFAICNYY333 pKa = 8.29 VKK335 pKa = 10.68 QMLLKK340 pKa = 10.04 PIHH343 pKa = 5.8 SWAMKK348 pKa = 9.19 ILSSIPMDD356 pKa = 3.29 GTFNQVAPLLRR367 pKa = 11.84 LAKK370 pKa = 10.01 IKK372 pKa = 9.4 RR373 pKa = 11.84 THH375 pKa = 6.33 VYY377 pKa = 10.79 SFDD380 pKa = 3.81 LKK382 pKa = 10.88 SATDD386 pKa = 3.7 RR387 pKa = 11.84 WPLPIIYY394 pKa = 8.59 TCVASFFGEE403 pKa = 4.89 TYY405 pKa = 10.54 ASSVVNSTLGLNTFRR420 pKa = 11.84 VDD422 pKa = 3.21 KK423 pKa = 10.3 PIVSRR428 pKa = 11.84 MSEE431 pKa = 3.5 IAFRR435 pKa = 11.84 CGQPLGYY442 pKa = 9.66 YY443 pKa = 10.17 GSWSLFALSHH453 pKa = 6.41 HH454 pKa = 5.92 LVVWLAADD462 pKa = 4.01 LAYY465 pKa = 9.79 PSRR468 pKa = 11.84 EE469 pKa = 4.01 TPFRR473 pKa = 11.84 DD474 pKa = 3.62 YY475 pKa = 11.52 AVLGDD480 pKa = 4.6 DD481 pKa = 5.12 VLIADD486 pKa = 4.03 TNVALEE492 pKa = 4.12 YY493 pKa = 10.65 KK494 pKa = 10.42 SLLSRR499 pKa = 11.84 LGVSISEE506 pKa = 4.31 SKK508 pKa = 10.88 SIISEE513 pKa = 3.85 TGAIEE518 pKa = 3.9 FAKK521 pKa = 10.29 KK522 pKa = 9.85 YY523 pKa = 7.65 WVKK526 pKa = 10.85 GMQVDD531 pKa = 4.51 LSPVSLKK538 pKa = 10.82 SLLGARR544 pKa = 11.84 HH545 pKa = 6.28 TIGLCQIGAKK555 pKa = 9.88 YY556 pKa = 10.76 DD557 pKa = 3.77 LDD559 pKa = 4.3 FNTLLRR565 pKa = 11.84 IGGAGYY571 pKa = 10.07 RR572 pKa = 11.84 VRR574 pKa = 11.84 SRR576 pKa = 11.84 QLSTLNRR583 pKa = 11.84 KK584 pKa = 6.75 WEE586 pKa = 4.38 KK587 pKa = 10.31 IRR589 pKa = 11.84 AVQVKK594 pKa = 8.3 LTGQRR599 pKa = 11.84 AQLPIEE605 pKa = 4.08 FWIGRR610 pKa = 11.84 GCPLDD615 pKa = 4.31 PYY617 pKa = 10.68 LKK619 pKa = 10.65 GKK621 pKa = 10.1 IIVYY625 pKa = 9.45 LRR627 pKa = 11.84 KK628 pKa = 9.56 EE629 pKa = 4.35 FKK631 pKa = 10.32 PKK633 pKa = 9.99 EE634 pKa = 3.93 LRR636 pKa = 11.84 LTPEE640 pKa = 4.0 GLLFDD645 pKa = 4.6 GEE647 pKa = 4.47 VEE649 pKa = 3.88 IAEE652 pKa = 4.04 RR653 pKa = 11.84 TVIHH657 pKa = 6.21 NWMKK661 pKa = 10.61 QWLKK665 pKa = 9.8 WIYY668 pKa = 8.85 WYY670 pKa = 11.05 YY671 pKa = 10.25 SVAMSPDD678 pKa = 3.1 VKK680 pKa = 10.72 LDD682 pKa = 3.32 QFFDD686 pKa = 3.72 APICATSWKK695 pKa = 10.12 RR696 pKa = 11.84 NQIDD700 pKa = 4.07 LNLKK704 pKa = 9.98 RR705 pKa = 11.84 FSALWRR711 pKa = 11.84 CYY713 pKa = 11.03 DD714 pKa = 4.07 MAVGWPKK721 pKa = 10.61 NYY723 pKa = 9.68 PWVLPATTVLKK734 pKa = 10.13 IDD736 pKa = 3.78 EE737 pKa = 4.94 LIKK740 pKa = 11.0 GGFSGTDD747 pKa = 3.42 FLMKK751 pKa = 9.96 PVEE754 pKa = 5.66 LIVQQNKK761 pKa = 7.23 GRR763 pKa = 11.84 KK764 pKa = 8.64 YY765 pKa = 9.81 II766 pKa = 3.87
Molecular weight: 87.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.184
IPC2_protein 9.311
IPC_protein 9.282
Toseland 9.926
ProMoST 9.648
Dawson 10.16
Bjellqvist 9.838
Wikipedia 10.321
Rodwell 10.526
Grimsley 10.233
Solomon 10.175
Lehninger 10.131
Nozaki 9.955
DTASelect 9.823
Thurlkill 9.999
EMBOSS 10.335
Sillero 10.072
Patrickios 9.663
IPC_peptide 10.16
IPC2_peptide 8.536
IPC2.peptide.svr19 8.216
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
766
766
766
766.0
87.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.005 ± 0.0
1.305 ± 0.0
3.786 ± 0.0
4.178 ± 0.0
4.7 ± 0.0
5.614 ± 0.0
1.567 ± 0.0
6.919 ± 0.0
6.919 ± 0.0
11.619 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.828 ± 0.0
2.611 ± 0.0
5.352 ± 0.0
2.611 ± 0.0
6.919 ± 0.0
8.747 ± 0.0
5.222 ± 0.0
6.789 ± 0.0
3.133 ± 0.0
4.178 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here