Tortoise microvirus 85

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6P6|A0A4P8W6P6_9VIRU DNA pilot protein OS=Tortoise microvirus 85 OX=2583193 PE=4 SV=1
MM1 pKa = 7.57IKK3 pKa = 10.58NLGGDD8 pKa = 3.58RR9 pKa = 11.84LGSGAKK15 pKa = 9.04MNQEE19 pKa = 3.57LHH21 pKa = 5.09GWSRR25 pKa = 11.84STHH28 pKa = 6.88DD29 pKa = 4.12LTTDD33 pKa = 2.85IATTMSIGTLLPIHH47 pKa = 6.73HH48 pKa = 6.48SVILAGDD55 pKa = 3.54TRR57 pKa = 11.84EE58 pKa = 4.0IDD60 pKa = 3.76LDD62 pKa = 3.98AMLLTHH68 pKa = 6.25PTEE71 pKa = 4.9GPLFGSFKK79 pKa = 10.36LQVDD83 pKa = 3.61VFKK86 pKa = 11.36ADD88 pKa = 3.17MRR90 pKa = 11.84LYY92 pKa = 9.97IAKK95 pKa = 10.11LHH97 pKa = 6.05MNLLEE102 pKa = 4.96KK103 pKa = 10.46GLTMSDD109 pKa = 2.51IKK111 pKa = 11.16LPLIEE116 pKa = 4.58MDD118 pKa = 4.44ANNIDD123 pKa = 3.55WTKK126 pKa = 11.34DD127 pKa = 3.23PNNQQINPSCIFKK140 pKa = 10.32YY141 pKa = 10.29LHH143 pKa = 6.61ISGLGYY149 pKa = 10.36NDD151 pKa = 3.46SFEE154 pKa = 4.14APRR157 pKa = 11.84RR158 pKa = 11.84YY159 pKa = 9.98FNAIPWLMYY168 pKa = 8.94WDD170 pKa = 3.74IYY172 pKa = 9.97KK173 pKa = 10.62NYY175 pKa = 9.48YY176 pKa = 9.98ANKK179 pKa = 8.39QEE181 pKa = 4.16TYY183 pKa = 9.95GWVIHH188 pKa = 5.88TGIGLNEE195 pKa = 4.56TITAIKK201 pKa = 9.99IKK203 pKa = 9.03TGSGAEE209 pKa = 4.08STIVEE214 pKa = 4.29APATGLYY221 pKa = 9.95PVTGFTKK228 pKa = 9.5ITIEE232 pKa = 3.95YY233 pKa = 10.54DD234 pKa = 3.24GAQPLLSTVMVNTTNGQQQLDD255 pKa = 3.6QLFTQITYY263 pKa = 10.78EE264 pKa = 4.03NDD266 pKa = 3.15DD267 pKa = 4.47KK268 pKa = 11.36IILEE272 pKa = 4.16IPNSNFNITNWLYY285 pKa = 11.17VEE287 pKa = 4.33NGVPITTVPRR297 pKa = 11.84LEE299 pKa = 4.56KK300 pKa = 10.76FPLTNLDD307 pKa = 4.29EE308 pKa = 5.08IKK310 pKa = 10.06MDD312 pKa = 3.37ILADD316 pKa = 3.54IKK318 pKa = 10.36STSAFTIKK326 pKa = 10.49YY327 pKa = 8.95DD328 pKa = 3.44DD329 pKa = 5.19HH330 pKa = 6.8IAPFSYY336 pKa = 10.74ALEE339 pKa = 4.25LTNGIYY345 pKa = 10.74SKK347 pKa = 10.15MYY349 pKa = 9.17SQEE352 pKa = 3.33GLAIKK357 pKa = 9.28TYY359 pKa = 10.74QSDD362 pKa = 5.1LFNNWLNTEE371 pKa = 4.36YY372 pKa = 10.38IDD374 pKa = 4.63GVNGINARR382 pKa = 11.84TAIQVDD388 pKa = 3.93VNGKK392 pKa = 7.7FTIDD396 pKa = 3.24NFLLKK401 pKa = 10.52EE402 pKa = 3.94KK403 pKa = 10.61LYY405 pKa = 11.26DD406 pKa = 3.48HH407 pKa = 7.37LNRR410 pKa = 11.84IAIAGGTVDD419 pKa = 6.07DD420 pKa = 4.39MMEE423 pKa = 3.84VTYY426 pKa = 10.64DD427 pKa = 3.14VSNRR431 pKa = 11.84TRR433 pKa = 11.84SEE435 pKa = 3.56IPTFEE440 pKa = 5.47GGMSKK445 pKa = 10.3EE446 pKa = 4.01LVFEE450 pKa = 4.03QVISNTATSTQPLGTIAGRR469 pKa = 11.84GTTQGHH475 pKa = 6.27KK476 pKa = 10.04GGKK479 pKa = 8.95IRR481 pKa = 11.84ITNDD485 pKa = 3.13DD486 pKa = 3.82TQHH489 pKa = 6.39AYY491 pKa = 11.31YY492 pKa = 9.65MVIASLTPRR501 pKa = 11.84IKK503 pKa = 10.59YY504 pKa = 9.5SQGNGWDD511 pKa = 4.11TNIKK515 pKa = 9.81TYY517 pKa = 11.33DD518 pKa = 4.12DD519 pKa = 3.52LHH521 pKa = 7.86KK522 pKa = 10.41PAFDD526 pKa = 3.52QIGFQDD532 pKa = 5.58LITDD536 pKa = 4.21QMAFWDD542 pKa = 5.04TYY544 pKa = 10.39CVSGGIAPPVFKK556 pKa = 10.84SAGKK560 pKa = 8.98QPAWINYY567 pKa = 4.47QTEE570 pKa = 3.77IDD572 pKa = 3.52RR573 pKa = 11.84VFGNFAIQSAEE584 pKa = 3.08GWMVLDD590 pKa = 5.9RR591 pKa = 11.84NYY593 pKa = 11.57DD594 pKa = 3.13WDD596 pKa = 4.36AEE598 pKa = 4.13NHH600 pKa = 5.66TIKK603 pKa = 11.01DD604 pKa = 3.6LTTYY608 pKa = 10.51IDD610 pKa = 3.49PEE612 pKa = 4.2KK613 pKa = 10.66YY614 pKa = 10.31NGVFAYY620 pKa = 10.05RR621 pKa = 11.84ALDD624 pKa = 3.67AQNIWAQFKK633 pKa = 10.72VRR635 pKa = 11.84DD636 pKa = 3.47KK637 pKa = 11.15AIRR640 pKa = 11.84KK641 pKa = 8.17MSANQIPRR649 pKa = 11.84MM650 pKa = 4.0

Molecular weight: 73.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W775|A0A4P8W775_9VIRU Uncharacterized protein OS=Tortoise microvirus 85 OX=2583193 PE=4 SV=1
MM1 pKa = 7.74EE2 pKa = 5.23CRR4 pKa = 11.84KK5 pKa = 10.04QKK7 pKa = 10.71ARR9 pKa = 11.84DD10 pKa = 3.32WQVRR14 pKa = 11.84LLEE17 pKa = 4.3DD18 pKa = 3.46VRR20 pKa = 11.84HH21 pKa = 4.88NTNGKK26 pKa = 10.07FITLTLSNEE35 pKa = 4.1NFKK38 pKa = 11.01KK39 pKa = 10.42LAYY42 pKa = 8.55EE43 pKa = 4.14ANKK46 pKa = 8.5KK47 pKa = 6.93TKK49 pKa = 9.73KK50 pKa = 10.15HH51 pKa = 6.04PIGKK55 pKa = 9.29KK56 pKa = 9.89EE57 pKa = 3.97YY58 pKa = 9.06IDD60 pKa = 3.65KK61 pKa = 10.72NGKK64 pKa = 8.68IRR66 pKa = 11.84IRR68 pKa = 11.84YY69 pKa = 7.74KK70 pKa = 10.47YY71 pKa = 9.24KK72 pKa = 10.41TITEE76 pKa = 4.16QIEE79 pKa = 3.91LRR81 pKa = 11.84GYY83 pKa = 8.16EE84 pKa = 4.16TDD86 pKa = 3.88NEE88 pKa = 4.22IAKK91 pKa = 10.06LAVRR95 pKa = 11.84RR96 pKa = 11.84FLEE99 pKa = 4.33RR100 pKa = 11.84WRR102 pKa = 11.84KK103 pKa = 6.95TYY105 pKa = 10.51KK106 pKa = 10.0KK107 pKa = 10.23SLRR110 pKa = 11.84HH111 pKa = 5.01WLVTEE116 pKa = 4.84LGHH119 pKa = 7.04NGTEE123 pKa = 4.22NIHH126 pKa = 5.86LHH128 pKa = 5.91GIVWTNEE135 pKa = 3.75PIEE138 pKa = 4.85AIRR141 pKa = 11.84KK142 pKa = 7.59HH143 pKa = 5.55WDD145 pKa = 3.05YY146 pKa = 11.65GYY148 pKa = 8.78IWPRR152 pKa = 11.84KK153 pKa = 6.36EE154 pKa = 3.67HH155 pKa = 6.17EE156 pKa = 4.38KK157 pKa = 10.66VIKK160 pKa = 10.66NYY162 pKa = 10.34VNEE165 pKa = 3.96RR166 pKa = 11.84TINYY170 pKa = 4.73ITKK173 pKa = 9.86YY174 pKa = 10.32VSKK177 pKa = 10.81QDD179 pKa = 3.9FQHH182 pKa = 6.7KK183 pKa = 8.95EE184 pKa = 3.64YY185 pKa = 10.71KK186 pKa = 10.58AIILTSAGIGAGYY199 pKa = 6.74MTRR202 pKa = 11.84MDD204 pKa = 3.97SKK206 pKa = 10.51LNKK209 pKa = 10.16YY210 pKa = 10.13NGEE213 pKa = 4.1DD214 pKa = 3.24TKK216 pKa = 11.18DD217 pKa = 3.59YY218 pKa = 10.16YY219 pKa = 9.41VTRR222 pKa = 11.84TGHH225 pKa = 6.51KK226 pKa = 9.69IKK228 pKa = 10.63IPIYY232 pKa = 8.74WRR234 pKa = 11.84NKK236 pKa = 8.63IYY238 pKa = 10.63NDD240 pKa = 3.53EE241 pKa = 3.98EE242 pKa = 4.82RR243 pKa = 11.84EE244 pKa = 4.04RR245 pKa = 11.84LWLHH249 pKa = 6.41KK250 pKa = 10.28LDD252 pKa = 5.03EE253 pKa = 4.86KK254 pKa = 10.95IRR256 pKa = 11.84WIGKK260 pKa = 7.83EE261 pKa = 4.06KK262 pKa = 10.25IDD264 pKa = 3.41ISLNEE269 pKa = 3.81EE270 pKa = 3.96EE271 pKa = 4.86YY272 pKa = 11.23YY273 pKa = 9.29KK274 pKa = 10.27TLHH277 pKa = 7.15HH278 pKa = 6.12YY279 pKa = 8.7RR280 pKa = 11.84QKK282 pKa = 10.75NARR285 pKa = 11.84LGYY288 pKa = 11.06GNGTKK293 pKa = 10.06DD294 pKa = 3.3WNRR297 pKa = 11.84KK298 pKa = 6.67QYY300 pKa = 10.52EE301 pKa = 4.0EE302 pKa = 3.81QRR304 pKa = 11.84RR305 pKa = 11.84ILLQQEE311 pKa = 4.71RR312 pKa = 11.84IRR314 pKa = 11.84ATEE317 pKa = 4.16EE318 pKa = 3.41IEE320 pKa = 5.65AYY322 pKa = 9.76PEE324 pKa = 3.67WVYY327 pKa = 11.48NSSGGSCAEE336 pKa = 4.12SATCFQVDD344 pKa = 3.62QRR346 pKa = 11.84EE347 pKa = 4.34HH348 pKa = 5.71

Molecular weight: 42.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1564

115

650

312.8

35.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.522 ± 1.221

0.32 ± 0.168

5.818 ± 0.886

7.801 ± 1.918

2.366 ± 0.747

7.353 ± 1.267

2.238 ± 0.697

8.376 ± 0.694

8.76 ± 1.733

7.289 ± 0.38

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.685 ± 0.757

6.394 ± 0.252

2.366 ± 0.751

5.179 ± 1.317

4.604 ± 1.139

4.476 ± 0.525

7.353 ± 0.829

3.772 ± 0.442

2.046 ± 0.375

4.284 ± 1.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski