Photobacterium aphoticum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Photobacterium

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4349 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J1GKN3|A0A0J1GKN3_9GAMM BOF domain-containing protein OS=Photobacterium aphoticum OX=754436 GN=ABT58_13535 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 9.0KK3 pKa = 8.22TLLALSVLAAGSAQAGINLYY23 pKa = 9.82DD24 pKa = 3.9ANGVTVDD31 pKa = 3.57LSGAVEE37 pKa = 4.0VQYY40 pKa = 10.89LQNIQDD46 pKa = 4.01KK47 pKa = 11.27NSDD50 pKa = 3.36ADD52 pKa = 3.86AGLHH56 pKa = 6.74LDD58 pKa = 5.68DD59 pKa = 6.75GDD61 pKa = 4.08LQLNTTIAVNEE72 pKa = 4.06QLNVVAGIGFAFEE85 pKa = 4.7KK86 pKa = 10.48RR87 pKa = 11.84DD88 pKa = 3.61VSNDD92 pKa = 3.48EE93 pKa = 3.88LWAGLSGDD101 pKa = 3.89FGTLTFGRR109 pKa = 11.84MLYY112 pKa = 10.13ISDD115 pKa = 3.89DD116 pKa = 3.51AGIGKK121 pKa = 9.92DD122 pKa = 3.55YY123 pKa = 11.05EE124 pKa = 4.44LGFGQVDD131 pKa = 4.1FVQTEE136 pKa = 4.07GNEE139 pKa = 4.09VIKK142 pKa = 10.83YY143 pKa = 10.23VFDD146 pKa = 3.59NGQFYY151 pKa = 10.75FGLSHH156 pKa = 7.62DD157 pKa = 5.12LDD159 pKa = 4.25TDD161 pKa = 3.72GSGASDD167 pKa = 3.71YY168 pKa = 11.4QNTDD172 pKa = 2.52GTVTDD177 pKa = 3.54VRR179 pKa = 11.84LGARR183 pKa = 11.84FADD186 pKa = 3.69LDD188 pKa = 3.69VRR190 pKa = 11.84GYY192 pKa = 11.02YY193 pKa = 8.13YY194 pKa = 10.6TGDD197 pKa = 4.56DD198 pKa = 3.52IQRR201 pKa = 11.84SADD204 pKa = 3.45QNGNPIFNSGTDD216 pKa = 2.9VDD218 pKa = 4.4AFNLEE223 pKa = 3.83AEE225 pKa = 4.43YY226 pKa = 11.8VMGQFAFAASYY237 pKa = 11.82GNFDD241 pKa = 3.5YY242 pKa = 11.62SNANGATYY250 pKa = 11.04SDD252 pKa = 3.2IDD254 pKa = 3.99VIEE257 pKa = 4.21VNGAYY262 pKa = 9.27TLGKK266 pKa = 8.35NTFALGYY273 pKa = 10.4NRR275 pKa = 11.84ADD277 pKa = 3.87DD278 pKa = 4.06SKK280 pKa = 11.63QDD282 pKa = 3.52VVTDD286 pKa = 3.41NVYY289 pKa = 11.46ANVTHH294 pKa = 6.15QLHH297 pKa = 6.73SNVKK301 pKa = 9.8VYY303 pKa = 11.26GEE305 pKa = 4.86LGWADD310 pKa = 3.41QDD312 pKa = 5.34GSDD315 pKa = 4.7LDD317 pKa = 4.03LGYY320 pKa = 10.93LVGMEE325 pKa = 4.19VKK327 pKa = 10.64FF328 pKa = 3.9

Molecular weight: 35.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J1GN04|A0A0J1GN04_9GAMM Uncharacterized protein OS=Photobacterium aphoticum OX=754436 GN=ABT58_10735 PE=4 SV=1
MM1 pKa = 7.34PRR3 pKa = 11.84HH4 pKa = 5.23VIKK7 pKa = 10.68RR8 pKa = 11.84FMPSHH13 pKa = 6.21EE14 pKa = 4.35LIKK17 pKa = 10.35RR18 pKa = 11.84QKK20 pKa = 9.84ALKK23 pKa = 10.18IFGNVLYY30 pKa = 10.72NPNLWCLNRR39 pKa = 11.84RR40 pKa = 11.84SASGAFAVGLFMAFVPLPSQMIMAAGLAILFSVNLPLSVCLVWISNPITMPVLFYY95 pKa = 10.95GAYY98 pKa = 9.47KK99 pKa = 10.2VGAWLMSTPHH109 pKa = 5.52QAFHH113 pKa = 7.45FEE115 pKa = 4.26LSWEE119 pKa = 4.2FLLHH123 pKa = 6.03QMNQIGPPFLLGCFVTGVTCALIGYY148 pKa = 8.25FGIRR152 pKa = 11.84GLWRR156 pKa = 11.84YY157 pKa = 9.89SVVRR161 pKa = 11.84SWQKK165 pKa = 10.2RR166 pKa = 11.84KK167 pKa = 9.73FRR169 pKa = 11.84VLRR172 pKa = 4.07

Molecular weight: 19.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4349

0

4349

1394034

35

4031

320.5

35.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.631 ± 0.047

1.084 ± 0.014

5.706 ± 0.041

5.851 ± 0.035

3.985 ± 0.026

6.969 ± 0.04

2.474 ± 0.024

5.923 ± 0.03

4.523 ± 0.035

10.285 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.878 ± 0.019

3.863 ± 0.026

4.22 ± 0.022

4.747 ± 0.03

4.724 ± 0.031

5.93 ± 0.03

5.682 ± 0.036

7.132 ± 0.033

1.319 ± 0.017

3.072 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski