Cyprinid herpesvirus 1
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 137 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7PC75|K7PC75_9VIRU Protein ORF70 OS=Cyprinid herpesvirus 1 OX=317858 GN=CyHV1_ORF70 PE=4 SV=1
MM1 pKa = 7.51 ALLWSLVFLLATCAISVTCQSVTWTRR27 pKa = 11.84 SSPCSPMGTTLVITGAYY44 pKa = 7.87 TMPTGQYY51 pKa = 9.84 AIAGAILSPNMDD63 pKa = 3.33 IKK65 pKa = 10.16 TVATVTTRR73 pKa = 11.84 ATVNLTPGNKK83 pKa = 8.56 GTVTFTLSGVRR94 pKa = 11.84 MSDD97 pKa = 2.76 ATFTGFALKK106 pKa = 10.81 VLTTTGASTTMSTLAPPITVATNPSIRR133 pKa = 11.84 VLRR136 pKa = 11.84 YY137 pKa = 9.26 NDD139 pKa = 3.32 YY140 pKa = 10.82 SLGGGTVLRR149 pKa = 11.84 CVQSCNSTINNHH161 pKa = 3.84 VWYY164 pKa = 10.64 KK165 pKa = 10.62 NGLKK169 pKa = 9.9 IGSAIGTDD177 pKa = 3.38 MTAVDD182 pKa = 3.98 SGLYY186 pKa = 8.53 TCEE189 pKa = 4.06 PDD191 pKa = 3.75 GSINTRR197 pKa = 11.84 SNSIWVGTPTYY208 pKa = 10.79 DD209 pKa = 5.0 CPFDD213 pKa = 3.71 LAKK216 pKa = 9.68 WCSSGFITRR225 pKa = 11.84 RR226 pKa = 11.84 HH227 pKa = 5.28 EE228 pKa = 4.59 NNVKK232 pKa = 10.32 AYY234 pKa = 10.43 NSDD237 pKa = 3.16 HH238 pKa = 6.09 TRR240 pKa = 11.84 TVGNTFVCGSSTDD253 pKa = 3.56 GTSPAISTRR262 pKa = 11.84 GMCPNRR268 pKa = 11.84 TTCTSEE274 pKa = 3.79 KK275 pKa = 8.61 TTMCANNNPVINVACGMVISYY296 pKa = 7.56 TCPTISPFEE305 pKa = 4.39 SIGIVSSSGSEE316 pKa = 3.94 FALSPVAGEE325 pKa = 4.26 RR326 pKa = 11.84 RR327 pKa = 11.84 LEE329 pKa = 4.04 TSTKK333 pKa = 9.87 GNVYY337 pKa = 10.23 KK338 pKa = 10.39 IWCGSDD344 pKa = 3.41 YY345 pKa = 11.25 EE346 pKa = 4.81 LVAPCGTSSSYY357 pKa = 10.65 SAHH360 pKa = 7.14 PSTFIPIGAGATWTTTGVTQSEE382 pKa = 4.49 TMIIAVGTVFNITCPDD398 pKa = 3.34 NTVVAWYY405 pKa = 10.08 KK406 pKa = 11.11 DD407 pKa = 3.18 KK408 pKa = 10.64 TLFSSLIGSRR418 pKa = 11.84 STKK421 pKa = 10.94 NMVKK425 pKa = 10.43 NLTADD430 pKa = 3.6 DD431 pKa = 4.01 YY432 pKa = 12.18 GMWEE436 pKa = 4.48 CVSFTTTGSTLGARR450 pKa = 11.84 TRR452 pKa = 11.84 LISISAPTTTVTTPLTTPSTTAATTAAATNATTAATTAAATNATTAATTAAATNATTAATTAATTAAATNATTAATTEE530 pKa = 4.0 AATNATTAATTAATTEE546 pKa = 4.0 AATNATTAATTASATNATTAATTEE570 pKa = 4.0 AATNATTAATTEE582 pKa = 4.0 AATNATTAATTEE594 pKa = 4.0 AATNATTAATTAATTEE610 pKa = 4.0 AATNATTAATTAATTEE626 pKa = 4.0 AATNATTAATTAATTEE642 pKa = 4.0 AATNATTAATTAATTEE658 pKa = 4.0 AATNATTAATTAATTEE674 pKa = 4.0 AATNATTAATTAATTEE690 pKa = 4.0 AATNATTEE698 pKa = 4.01 AATNATTEE706 pKa = 4.01 AATNATMSPTTPDD719 pKa = 3.06 ASNATTDD726 pKa = 3.32 ASTPDD731 pKa = 3.28 ASNATTAATTEE742 pKa = 4.26 DD743 pKa = 3.42 ATNATTEE750 pKa = 3.97 AATNATMSPATPDD763 pKa = 3.2 ASNATTDD770 pKa = 3.32 ASTPDD775 pKa = 3.28 ASNATTAATTPDD787 pKa = 3.54 ASNATTDD794 pKa = 3.3 AATNATMSPTTPDD807 pKa = 3.06 ASNATTDD814 pKa = 3.12 ASTPDD819 pKa = 3.48 TSNATTDD826 pKa = 2.83 ATTAGNTPATTPGTPEE842 pKa = 3.96 TTTGRR847 pKa = 11.84 PLTTPSPTTQSTPINPTGLSTLTMAAIIGGAAGGAALIAAIVAVCVIHH895 pKa = 7.04 NGII898 pKa = 4.04
Molecular weight: 89.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.254
IPC2_protein 4.533
IPC_protein 4.482
Toseland 4.317
ProMoST 4.609
Dawson 4.444
Bjellqvist 4.596
Wikipedia 4.329
Rodwell 4.329
Grimsley 4.228
Solomon 4.444
Lehninger 4.393
Nozaki 4.546
DTASelect 4.736
Thurlkill 4.329
EMBOSS 4.342
Sillero 4.609
Patrickios 2.015
IPC_peptide 4.444
IPC2_peptide 4.596
IPC2.peptide.svr19 4.515
Protein with the highest isoelectric point:
>tr|K7PC85|K7PC85_9VIRU Protein ORF85 OS=Cyprinid herpesvirus 1 OX=317858 GN=CyHV1_ORF85 PE=4 SV=1
MM1 pKa = 7.6 EE2 pKa = 5.8 LPRR5 pKa = 11.84 VAIPAEE11 pKa = 3.94 ARR13 pKa = 11.84 EE14 pKa = 4.04 LGITKK19 pKa = 8.84 TLSEE23 pKa = 5.39 LYY25 pKa = 9.43 PSHH28 pKa = 7.01 RR29 pKa = 11.84 FYY31 pKa = 11.06 RR32 pKa = 11.84 GQRR35 pKa = 11.84 RR36 pKa = 11.84 IAIKK40 pKa = 10.82 LEE42 pKa = 4.02 VEE44 pKa = 4.05 GDD46 pKa = 3.69 EE47 pKa = 4.01 QDD49 pKa = 3.78 LVVRR53 pKa = 11.84 LTKK56 pKa = 10.09 SLRR59 pKa = 11.84 GRR61 pKa = 11.84 VWTKK65 pKa = 9.26 PQLRR69 pKa = 11.84 AQILSLLPGLSGQQGEE85 pKa = 4.78 DD86 pKa = 3.0 ACQAAAEE93 pKa = 4.24 ALTRR97 pKa = 11.84 SAPFTLKK104 pKa = 10.2 SVRR107 pKa = 11.84 SALISTRR114 pKa = 11.84 FFARR118 pKa = 11.84 MGSLVDD124 pKa = 3.54 SRR126 pKa = 11.84 VKK128 pKa = 10.77 RR129 pKa = 11.84 EE130 pKa = 3.75 FEE132 pKa = 3.97 CGKK135 pKa = 10.79 GYY137 pKa = 10.65 LVDD140 pKa = 3.81 HH141 pKa = 6.75 LKK143 pKa = 10.66 AVYY146 pKa = 9.29 GWRR149 pKa = 11.84 PLASAVNVTSKK160 pKa = 10.86 VPLGHH165 pKa = 5.75 SCEE168 pKa = 4.35 RR169 pKa = 11.84 CHH171 pKa = 6.98 SVCSRR176 pKa = 11.84 QVPSHH181 pKa = 5.44 KK182 pKa = 9.65 TEE184 pKa = 4.17 LDD186 pKa = 3.11 AVAVDD191 pKa = 3.74 EE192 pKa = 4.59 RR193 pKa = 11.84 GEE195 pKa = 4.1 YY196 pKa = 10.82 ALLEE200 pKa = 4.22 IKK202 pKa = 9.31 TRR204 pKa = 11.84 SSSTVTAALLRR215 pKa = 11.84 RR216 pKa = 11.84 YY217 pKa = 8.5 QVQTWIGEE225 pKa = 4.02 MMFRR229 pKa = 11.84 NTYY232 pKa = 9.92 GMCTSKK238 pKa = 10.76 AIHH241 pKa = 6.37 SYY243 pKa = 10.37 IVFVDD248 pKa = 4.0 PCSYY252 pKa = 10.36 TVDD255 pKa = 3.35 RR256 pKa = 11.84 VICVDD261 pKa = 3.53 SKK263 pKa = 11.63 LNRR266 pKa = 11.84 PSPRR270 pKa = 11.84 LFALFPSLLNLCQAKK285 pKa = 10.38 LSAPRR290 pKa = 11.84 VLLRR294 pKa = 11.84 PPAGRR299 pKa = 11.84 PSRR302 pKa = 11.84 PCASGAAVKK311 pKa = 10.41 RR312 pKa = 11.84 KK313 pKa = 9.69 RR314 pKa = 11.84 PSGAAAAEE322 pKa = 4.54 KK323 pKa = 10.19 KK324 pKa = 10.33 RR325 pKa = 11.84 KK326 pKa = 9.07 AA327 pKa = 3.43
Molecular weight: 36.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.269
IPC2_protein 9.326
IPC_protein 9.487
Toseland 10.16
ProMoST 9.823
Dawson 10.335
Bjellqvist 10.028
Wikipedia 10.496
Rodwell 10.672
Grimsley 10.394
Solomon 10.379
Lehninger 10.335
Nozaki 10.233
DTASelect 9.999
Thurlkill 10.204
EMBOSS 10.57
Sillero 10.262
Patrickios 10.292
IPC_peptide 10.379
IPC2_peptide 9.077
IPC2.peptide.svr19 8.212
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
137
0
137
65611
64
3611
478.9
53.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.241 ± 0.257
2.55 ± 0.116
5.394 ± 0.11
6.243 ± 0.211
3.51 ± 0.126
5.587 ± 0.118
2.401 ± 0.092
3.824 ± 0.108
4.928 ± 0.173
9.128 ± 0.186
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.716 ± 0.087
3.64 ± 0.153
5.883 ± 0.212
3.597 ± 0.113
5.991 ± 0.195
8.092 ± 0.172
6.776 ± 0.323
6.909 ± 0.132
1.468 ± 0.075
3.123 ± 0.094
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here