Kafue kinda chacma baboon virus
Average proteome isoelectric point is 7.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Y0DFZ4|A0A0Y0DFZ4_9NIDO E protein OS=Kafue kinda chacma baboon virus OX=1823757 GN=ORF2a PE=4 SV=1
MM1 pKa = 7.85 ASGLHH6 pKa = 5.74 VRR8 pKa = 11.84 LISCLCVFYY17 pKa = 10.68 LVRR20 pKa = 11.84 SSGANINSTDD30 pKa = 3.57 TAPEE34 pKa = 5.09 GICFLLPVKK43 pKa = 10.75 HH44 pKa = 6.04 NVLVNISLHH53 pKa = 5.68 TLFCTNDD60 pKa = 3.32 GAISMEE66 pKa = 3.55 VDD68 pKa = 3.17 EE69 pKa = 4.92 NHH71 pKa = 6.93 FGNDD75 pKa = 3.55 DD76 pKa = 3.83 CPLSGFKK83 pKa = 10.25 PHH85 pKa = 7.02 GSTTGKK91 pKa = 10.36 YY92 pKa = 10.65 GSFLHH97 pKa = 6.38 MSDD100 pKa = 3.79 INFPLNLTTDD110 pKa = 3.81 PSHH113 pKa = 8.29 VYY115 pKa = 8.44 ITILLTYY122 pKa = 10.64 LMANFPQVLIPNHH135 pKa = 5.39 NTSLPLALNATVTNTTWQFCINSTNIPSVGSGPIVDD171 pKa = 5.61 LYY173 pKa = 9.03 TTGPPWGLYY182 pKa = 9.59 YY183 pKa = 9.94 MEE185 pKa = 5.38 LLRR188 pKa = 11.84 PFLLSLLMLGLSHH201 pKa = 7.63 II202 pKa = 4.67
Molecular weight: 22.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.497
IPC2_protein 5.41
IPC_protein 5.448
Toseland 5.919
ProMoST 5.83
Dawson 5.753
Bjellqvist 5.741
Wikipedia 5.779
Rodwell 5.741
Grimsley 6.084
Solomon 5.753
Lehninger 5.741
Nozaki 6.033
DTASelect 6.224
Thurlkill 6.237
EMBOSS 6.211
Sillero 6.122
Patrickios 0.426
IPC_peptide 5.766
IPC2_peptide 6.16
IPC2.peptide.svr19 6.038
Protein with the highest isoelectric point:
>tr|A0A0Y0BL84|A0A0Y0BL84_9NIDO N protein OS=Kafue kinda chacma baboon virus OX=1823757 GN=ORF7 PE=4 SV=1
MM1 pKa = 7.24 VSSICSDD8 pKa = 3.31 PGYY11 pKa = 7.77 TTIAFTAAPIVIACLRR27 pKa = 11.84 LFRR30 pKa = 11.84 PCLRR34 pKa = 11.84 GFFCALCIATLAYY47 pKa = 10.21 AATAFQEE54 pKa = 4.31 HH55 pKa = 6.43 SLATIVTISFACIYY69 pKa = 10.55 CGFKK73 pKa = 10.53 LLQWIIIRR81 pKa = 11.84 FRR83 pKa = 11.84 MCRR86 pKa = 11.84 LGPGYY91 pKa = 10.13 ILSSPNHH98 pKa = 5.95 VDD100 pKa = 2.97 SSLGRR105 pKa = 11.84 YY106 pKa = 7.8 PITGTGSSAIVTRR119 pKa = 11.84 RR120 pKa = 11.84 SGMTIANNQLIPDD133 pKa = 4.48 VKK135 pKa = 10.86 RR136 pKa = 11.84 MVLAGKK142 pKa = 9.75 IATKK146 pKa = 10.28 KK147 pKa = 10.64 GLVNLRR153 pKa = 11.84 KK154 pKa = 10.28 YY155 pKa = 10.34 GWQKK159 pKa = 9.55 TKK161 pKa = 11.02
Molecular weight: 17.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 9.414
IPC_protein 9.531
Toseland 9.94
ProMoST 9.794
Dawson 10.189
Bjellqvist 9.955
Wikipedia 10.379
Rodwell 10.496
Grimsley 10.262
Solomon 10.233
Lehninger 10.189
Nozaki 10.131
DTASelect 9.911
Thurlkill 10.043
EMBOSS 10.365
Sillero 10.145
Patrickios 9.926
IPC_peptide 10.218
IPC2_peptide 9.268
IPC2.peptide.svr19 8.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
8350
50
3535
596.4
65.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.287 ± 0.273
3.102 ± 0.157
4.084 ± 0.514
3.377 ± 0.338
4.683 ± 0.284
6.982 ± 0.551
3.054 ± 0.334
5.21 ± 0.427
3.796 ± 0.302
10.527 ± 0.549
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.617 ± 0.109
3.593 ± 0.342
6.671 ± 0.531
3.114 ± 0.362
4.659 ± 0.295
7.988 ± 0.299
6.743 ± 0.389
7.593 ± 0.365
1.102 ± 0.196
3.82 ± 0.1
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here