Tropicimonas sediminicola
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4801 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A239GXR9|A0A239GXR9_9RHOB Peptide-methionine (R)-S-oxide reductase OS=Tropicimonas sediminicola OX=1031541 GN=SAMN05421757_103157 PE=4 SV=1
MM1 pKa = 7.65 LRR3 pKa = 11.84 QLVFASLVMLGAGPGTAQMTADD25 pKa = 3.48 VRR27 pKa = 11.84 FQSGNYY33 pKa = 7.46 GTMVSGTITGDD44 pKa = 3.06 EE45 pKa = 4.23 YY46 pKa = 11.28 FDD48 pKa = 3.84 YY49 pKa = 11.02 RR50 pKa = 11.84 LGASGGQQMFVDD62 pKa = 4.76 LSVAGTNGNGSVFFNILPPGSDD84 pKa = 2.8 GVAIYY89 pKa = 10.44 NSSMDD94 pKa = 3.74 GNSTTVEE101 pKa = 4.14 LPSDD105 pKa = 3.49 GTYY108 pKa = 10.13 TIRR111 pKa = 11.84 VYY113 pKa = 11.57 LMGNDD118 pKa = 3.41 RR119 pKa = 11.84 DD120 pKa = 3.83 AGATVGYY127 pKa = 10.2 NVDD130 pKa = 4.85 LSIQQ134 pKa = 3.33
Molecular weight: 14.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.872
IPC_protein 3.821
Toseland 3.592
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.834
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.973
DTASelect 4.266
Thurlkill 3.681
EMBOSS 3.846
Sillero 3.948
Patrickios 0.782
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.828
Protein with the highest isoelectric point:
>tr|A0A239IFC1|A0A239IFC1_9RHOB 30S ribosomal protein S5 OS=Tropicimonas sediminicola OX=1031541 GN=rpsE PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.34 GGRR28 pKa = 11.84 KK29 pKa = 8.8 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4801
0
4801
1534549
30
3803
319.6
34.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.499 ± 0.046
0.882 ± 0.011
5.96 ± 0.035
6.28 ± 0.03
3.763 ± 0.024
8.971 ± 0.037
2.012 ± 0.018
4.992 ± 0.028
2.689 ± 0.025
10.225 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.683 ± 0.017
2.433 ± 0.023
5.276 ± 0.027
2.886 ± 0.019
6.96 ± 0.041
5.194 ± 0.026
5.356 ± 0.024
7.352 ± 0.028
1.417 ± 0.015
2.169 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here