Lake Sarah-associated circular virus-50

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126G9T2|A0A126G9T2_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-50 OX=1685780 PE=4 SV=1
MM1 pKa = 7.0VKK3 pKa = 10.3SRR5 pKa = 11.84GWCYY9 pKa = 9.01TANNYY14 pKa = 7.74TKK16 pKa = 10.64EE17 pKa = 4.21EE18 pKa = 4.27YY19 pKa = 10.19EE20 pKa = 4.13SLEE23 pKa = 4.34KK24 pKa = 10.67YY25 pKa = 10.31DD26 pKa = 3.84CGYY29 pKa = 10.8HH30 pKa = 7.15VMGKK34 pKa = 9.39EE35 pKa = 3.97VGEE38 pKa = 4.05QGTPHH43 pKa = 5.46IQGYY47 pKa = 9.8IEE49 pKa = 3.91FTNAKK54 pKa = 10.2RR55 pKa = 11.84FDD57 pKa = 3.76TLHH60 pKa = 6.83ADD62 pKa = 3.77FPKK65 pKa = 10.38IHH67 pKa = 6.46WEE69 pKa = 3.93PRR71 pKa = 11.84KK72 pKa = 8.82GTQQQAIDD80 pKa = 3.71YY81 pKa = 9.0CMKK84 pKa = 10.7GGDD87 pKa = 3.72FVEE90 pKa = 4.92IGTKK94 pKa = 9.74KK95 pKa = 10.65DD96 pKa = 2.97QGKK99 pKa = 8.01RR100 pKa = 11.84TDD102 pKa = 3.18IEE104 pKa = 4.45AVIKK108 pKa = 10.32DD109 pKa = 3.4INDD112 pKa = 3.44KK113 pKa = 10.84VFSPNNHH120 pKa = 4.61AVAYY124 pKa = 9.17IKK126 pKa = 10.05YY127 pKa = 10.12AKK129 pKa = 10.76GIDD132 pKa = 3.63CYY134 pKa = 11.64ANYY137 pKa = 10.53KK138 pKa = 9.95LLPRR142 pKa = 11.84TVKK145 pKa = 9.22PTVEE149 pKa = 3.41WRR151 pKa = 11.84YY152 pKa = 10.73GLTGSGKK159 pKa = 7.5TRR161 pKa = 11.84EE162 pKa = 4.0PYY164 pKa = 9.42EE165 pKa = 3.72RR166 pKa = 11.84HH167 pKa = 5.93INSVYY172 pKa = 10.63IKK174 pKa = 10.54DD175 pKa = 3.42GTMWWNNYY183 pKa = 5.7TQQEE187 pKa = 4.1AVIIDD192 pKa = 4.55DD193 pKa = 5.79FDD195 pKa = 3.85GHH197 pKa = 5.22WPYY200 pKa = 11.46RR201 pKa = 11.84DD202 pKa = 3.7FLRR205 pKa = 11.84LLDD208 pKa = 4.28RR209 pKa = 11.84YY210 pKa = 9.52PYY212 pKa = 9.34QGQYY216 pKa = 9.58KK217 pKa = 9.59GGYY220 pKa = 8.29VNISSAYY227 pKa = 9.45IYY229 pKa = 8.14ITCEE233 pKa = 3.79FSPEE237 pKa = 4.18TFWRR241 pKa = 11.84GNEE244 pKa = 3.65LAQVIRR250 pKa = 11.84RR251 pKa = 11.84IDD253 pKa = 3.75TITLCRR259 pKa = 11.84INEE262 pKa = 4.23PVPVMVIDD270 pKa = 4.83DD271 pKa = 4.26LVDD274 pKa = 3.08

Molecular weight: 32.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126G9T2|A0A126G9T2_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-50 OX=1685780 PE=4 SV=1
MM1 pKa = 8.01PYY3 pKa = 11.14AKK5 pKa = 9.25MVKK8 pKa = 9.9GRR10 pKa = 11.84VAARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 5.32PSKK20 pKa = 10.8KK21 pKa = 9.16YY22 pKa = 9.86VKK24 pKa = 8.2PTKK27 pKa = 10.51SFTEE31 pKa = 4.06KK32 pKa = 9.97VVKK35 pKa = 10.35VINRR39 pKa = 11.84RR40 pKa = 11.84LDD42 pKa = 3.6VKK44 pKa = 10.89HH45 pKa = 6.76LDD47 pKa = 3.26AGYY50 pKa = 9.67GATAIVLRR58 pKa = 11.84NNTGPVAPTIVEE70 pKa = 4.51LAQSLIGTIGQGPGEE85 pKa = 4.19YY86 pKa = 9.9EE87 pKa = 3.73RR88 pKa = 11.84LGNDD92 pKa = 2.83IRR94 pKa = 11.84VKK96 pKa = 9.68SCIMRR101 pKa = 11.84GTLYY105 pKa = 9.33NTNATQRR112 pKa = 11.84PFYY115 pKa = 10.72IKK117 pKa = 10.22MFLLKK122 pKa = 10.69NKK124 pKa = 9.26LVPAADD130 pKa = 3.55TFNNLFEE137 pKa = 4.59GTTGNPTNQLSDD149 pKa = 3.08LTRR152 pKa = 11.84RR153 pKa = 11.84VNTDD157 pKa = 2.64EE158 pKa = 3.79YY159 pKa = 10.57TYY161 pKa = 10.54IASRR165 pKa = 11.84TFKK168 pKa = 10.52LGPSTATTNPNNDD181 pKa = 2.98FKK183 pKa = 10.92MSQFWRR189 pKa = 11.84IDD191 pKa = 3.27LTKK194 pKa = 10.38HH195 pKa = 4.73MKK197 pKa = 8.73IVKK200 pKa = 10.06YY201 pKa = 10.05PIDD204 pKa = 3.91GNGTVSQPASLFLVFVGAYY223 pKa = 10.04ADD225 pKa = 3.52NTAITFGAYY234 pKa = 7.96TGPAVSVSYY243 pKa = 11.16EE244 pKa = 3.8MTLDD248 pKa = 3.54FTDD251 pKa = 3.23AA252 pKa = 3.95

Molecular weight: 27.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

526

252

274

263.0

30.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.084 ± 1.191

1.331 ± 0.601

6.084 ± 0.85

4.753 ± 1.27

3.992 ± 0.495

7.414 ± 0.175

1.901 ± 0.457

6.654 ± 0.961

7.224 ± 0.052

5.513 ± 1.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.281 ± 0.319

5.703 ± 0.67

4.943 ± 0.394

3.232 ± 0.547

5.703 ± 0.16

3.802 ± 0.617

8.555 ± 1.388

6.844 ± 0.447

1.521 ± 0.723

6.464 ± 1.094
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski