Amino acid dipepetide frequency for Streptomyces sp. Ru72

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.35AlaAla: 20.35 ± 0.142	1.094AlaCys: 1.094 ± 0.025	7.836AlaAsp: 7.836 ± 0.065	8.382AlaGlu: 8.382 ± 0.085	3.502AlaPhe: 3.502 ± 0.042	12.458AlaGly: 12.458 ± 0.086	2.929AlaHis: 2.929 ± 0.036	3.6AlaIle: 3.6 ± 0.042	2.884AlaLys: 2.884 ± 0.049	14.287AlaLeu: 14.287 ± 0.116	2.418AlaMet: 2.418 ± 0.033	1.999AlaAsn: 1.999 ± 0.031	6.874AlaPro: 6.874 ± 0.07	3.85AlaGln: 3.85 ± 0.043	10.102AlaArg: 10.102 ± 0.081	6.059AlaSer: 6.059 ± 0.054	7.149AlaThr: 7.149 ± 0.061	12.132AlaVal: 12.132 ± 0.077	1.87AlaTrp: 1.87 ± 0.028	2.904AlaTyr: 2.904 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	1.092CysAla: 1.092 ± 0.025	0.115CysCys: 0.115 ± 0.008	0.465CysAsp: 0.465 ± 0.015	0.409CysGlu: 0.409 ± 0.013	0.235CysPhe: 0.235 ± 0.009	0.988CysGly: 0.988 ± 0.023	0.195CysHis: 0.195 ± 0.009	0.172CysIle: 0.172 ± 0.009	0.103CysLys: 0.103 ± 0.008	0.804CysLeu: 0.804 ± 0.02	0.123CysMet: 0.123 ± 0.008	0.153CysAsn: 0.153 ± 0.008	0.484CysPro: 0.484 ± 0.015	0.17CysGln: 0.17 ± 0.009	0.598CysArg: 0.598 ± 0.019	0.452CysSer: 0.452 ± 0.015	0.546CysThr: 0.546 ± 0.015	0.676CysVal: 0.676 ± 0.016	0.138CysTrp: 0.138 ± 0.008	0.161CysTyr: 0.161 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.343AspAla: 7.343 ± 0.064	0.435AspCys: 0.435 ± 0.014	3.448AspAsp: 3.448 ± 0.043	3.688AspGlu: 3.688 ± 0.044	1.681AspPhe: 1.681 ± 0.027	6.28AspGly: 6.28 ± 0.059	1.43AspHis: 1.43 ± 0.025	1.932AspIle: 1.932 ± 0.029	1.227AspLys: 1.227 ± 0.023	6.14AspLeu: 6.14 ± 0.058	0.822AspMet: 0.822 ± 0.018	1.006AspAsn: 1.006 ± 0.024	4.404AspPro: 4.404 ± 0.048	1.548AspGln: 1.548 ± 0.027	4.687AspArg: 4.687 ± 0.051	2.566AspSer: 2.566 ± 0.037	3.229AspThr: 3.229 ± 0.039	4.77AspVal: 4.77 ± 0.045	1.025AspTrp: 1.025 ± 0.023	1.161AspTyr: 1.161 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	7.235GluAla: 7.235 ± 0.089	0.355GluCys: 0.355 ± 0.014	2.788GluAsp: 2.788 ± 0.037	3.689GluGlu: 3.689 ± 0.048	1.397GluPhe: 1.397 ± 0.022	4.283GluGly: 4.283 ± 0.046	1.489GluHis: 1.489 ± 0.028	2.149GluIle: 2.149 ± 0.037	1.475GluLys: 1.475 ± 0.031	6.486GluLeu: 6.486 ± 0.064	0.882GluMet: 0.882 ± 0.021	1.024GluAsn: 1.024 ± 0.021	3.3GluPro: 3.3 ± 0.043	2.234GluGln: 2.234 ± 0.035	5.397GluArg: 5.397 ± 0.061	2.487GluSer: 2.487 ± 0.032	2.786GluThr: 2.786 ± 0.032	4.351GluVal: 4.351 ± 0.051	0.749GluTrp: 0.749 ± 0.017	1.143GluTyr: 1.143 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.617PheAla: 3.617 ± 0.045	0.273PheCys: 0.273 ± 0.011	1.933PheAsp: 1.933 ± 0.038	1.409PheGlu: 1.409 ± 0.025	0.904PhePhe: 0.904 ± 0.024	3.048PheGly: 3.048 ± 0.039	0.631PheHis: 0.631 ± 0.02	0.709PheIle: 0.709 ± 0.017	0.568PheLys: 0.568 ± 0.018	2.658PheLeu: 2.658 ± 0.043	0.416PheMet: 0.416 ± 0.014	0.6PheAsn: 0.6 ± 0.016	1.403PhePro: 1.403 ± 0.025	0.747PheGln: 0.747 ± 0.016	1.84PheArg: 1.84 ± 0.027	1.473PheSer: 1.473 ± 0.025	2.072PheThr: 2.072 ± 0.031	2.298PheVal: 2.298 ± 0.034	0.463PheTrp: 0.463 ± 0.012	0.617PheTyr: 0.617 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	10.728GlyAla: 10.728 ± 0.078	0.869GlyCys: 0.869 ± 0.023	5.024GlyAsp: 5.024 ± 0.054	4.842GlyGlu: 4.842 ± 0.049	2.908GlyPhe: 2.908 ± 0.035	8.707GlyGly: 8.707 ± 0.081	2.376GlyHis: 2.376 ± 0.033	3.571GlyIle: 3.571 ± 0.038	2.456GlyLys: 2.456 ± 0.039	9.396GlyLeu: 9.396 ± 0.077	1.954GlyMet: 1.954 ± 0.031	1.909GlyAsn: 1.909 ± 0.037	5.033GlyPro: 5.033 ± 0.055	2.674GlyGln: 2.674 ± 0.041	7.7GlyArg: 7.7 ± 0.059	5.528GlySer: 5.528 ± 0.063	6.569GlyThr: 6.569 ± 0.07	7.484GlyVal: 7.484 ± 0.063	1.744GlyTrp: 1.744 ± 0.031	2.375GlyTyr: 2.375 ± 0.036	0.0GlyXaa: 0.0 ± 0.0
His	2.731HisAla: 2.731 ± 0.032	0.22HisCys: 0.22 ± 0.01	1.343HisAsp: 1.343 ± 0.022	1.222HisGlu: 1.222 ± 0.024	0.673HisPhe: 0.673 ± 0.019	2.49HisGly: 2.49 ± 0.033	0.714HisHis: 0.714 ± 0.019	0.72HisIle: 0.72 ± 0.016	0.384HisLys: 0.384 ± 0.014	2.46HisLeu: 2.46 ± 0.036	0.33HisMet: 0.33 ± 0.012	0.394HisAsn: 0.394 ± 0.014	1.875HisPro: 1.875 ± 0.028	0.67HisGln: 0.67 ± 0.018	2.142HisArg: 2.142 ± 0.036	1.067HisSer: 1.067 ± 0.023	1.392HisThr: 1.392 ± 0.025	1.73HisVal: 1.73 ± 0.025	0.402HisTrp: 0.402 ± 0.013	0.522HisTyr: 0.522 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.9IleAla: 4.9 ± 0.054	0.281IleCys: 0.281 ± 0.011	2.31IleAsp: 2.31 ± 0.033	1.942IleGlu: 1.942 ± 0.031	0.715IlePhe: 0.715 ± 0.019	3.605IleGly: 3.605 ± 0.045	0.651IleHis: 0.651 ± 0.017	0.914IleIle: 0.914 ± 0.022	0.782IleLys: 0.782 ± 0.021	2.475IleLeu: 2.475 ± 0.04	0.473IleMet: 0.473 ± 0.014	0.754IleAsn: 0.754 ± 0.016	1.892IlePro: 1.892 ± 0.031	0.76IleGln: 0.76 ± 0.018	2.276IleArg: 2.276 ± 0.035	1.683IleSer: 1.683 ± 0.03	2.28IleThr: 2.28 ± 0.034	2.871IleVal: 2.871 ± 0.042	0.418IleTrp: 0.418 ± 0.012	0.564IleTyr: 0.564 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	3.067LysAla: 3.067 ± 0.049	0.128LysCys: 0.128 ± 0.007	1.44LysAsp: 1.44 ± 0.028	1.231LysGlu: 1.231 ± 0.025	0.468LysPhe: 0.468 ± 0.015	1.932LysGly: 1.932 ± 0.034	0.458LysHis: 0.458 ± 0.014	0.904LysIle: 0.904 ± 0.024	0.881LysLys: 0.881 ± 0.029	2.049LysLeu: 2.049 ± 0.038	0.37LysMet: 0.37 ± 0.014	0.555LysAsn: 0.555 ± 0.018	1.384LysPro: 1.384 ± 0.026	0.788LysGln: 0.788 ± 0.021	1.433LysArg: 1.433 ± 0.024	1.182LysSer: 1.182 ± 0.024	1.364LysThr: 1.364 ± 0.03	2.013LysVal: 2.013 ± 0.038	0.297LysTrp: 0.297 ± 0.01	0.508LysTyr: 0.508 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	14.719LeuAla: 14.719 ± 0.108	0.873LeuCys: 0.873 ± 0.017	6.464LeuAsp: 6.464 ± 0.061	4.71LeuGlu: 4.71 ± 0.059	2.653LeuPhe: 2.653 ± 0.04	9.029LeuGly: 9.029 ± 0.072	2.357LeuHis: 2.357 ± 0.034	3.4LeuIle: 3.4 ± 0.046	2.246LeuLys: 2.246 ± 0.034	11.225LeuLeu: 11.225 ± 0.092	1.657LeuMet: 1.657 ± 0.03	1.726LeuAsn: 1.726 ± 0.029	6.443LeuPro: 6.443 ± 0.062	2.452LeuGln: 2.452 ± 0.034	8.434LeuArg: 8.434 ± 0.079	5.275LeuSer: 5.275 ± 0.047	6.906LeuThr: 6.906 ± 0.055	8.859LeuVal: 8.859 ± 0.08	1.346LeuTrp: 1.346 ± 0.029	1.964LeuTyr: 1.964 ± 0.031	0.0LeuXaa: 0.0 ± 0.0
Met	2.275MetAla: 2.275 ± 0.035	0.139MetCys: 0.139 ± 0.008	0.891MetAsp: 0.891 ± 0.02	0.75MetGlu: 0.75 ± 0.021	0.436MetPhe: 0.436 ± 0.014	1.3MetGly: 1.3 ± 0.028	0.359MetHis: 0.359 ± 0.011	0.672MetIle: 0.672 ± 0.017	0.418MetLys: 0.418 ± 0.014	1.64MetLeu: 1.64 ± 0.028	0.294MetMet: 0.294 ± 0.01	0.448MetAsn: 0.448 ± 0.013	1.131MetPro: 1.131 ± 0.024	0.459MetGln: 0.459 ± 0.016	1.405MetArg: 1.405 ± 0.027	1.313MetSer: 1.313 ± 0.024	1.574MetThr: 1.574 ± 0.026	1.281MetVal: 1.281 ± 0.022	0.22MetTrp: 0.22 ± 0.009	0.334MetTyr: 0.334 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.281AsnAla: 2.281 ± 0.037	0.169AsnCys: 0.169 ± 0.008	0.99AsnAsp: 0.99 ± 0.02	0.832AsnGlu: 0.832 ± 0.02	0.516AsnPhe: 0.516 ± 0.013	2.06AsnGly: 2.06 ± 0.042	0.411AsnHis: 0.411 ± 0.014	0.685AsnIle: 0.685 ± 0.018	0.451AsnLys: 0.451 ± 0.015	1.726AsnLeu: 1.726 ± 0.027	0.313AsnMet: 0.313 ± 0.012	0.48AsnAsn: 0.48 ± 0.019	1.414AsnPro: 1.414 ± 0.026	0.605AsnGln: 0.605 ± 0.017	1.253AsnArg: 1.253 ± 0.024	1.024AsnSer: 1.024 ± 0.025	1.183AsnThr: 1.183 ± 0.026	1.489AsnVal: 1.489 ± 0.028	0.314AsnTrp: 0.314 ± 0.011	0.453AsnTyr: 0.453 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	8.087ProAla: 8.087 ± 0.074	0.348ProCys: 0.348 ± 0.014	4.398ProAsp: 4.398 ± 0.045	4.272ProGlu: 4.272 ± 0.049	1.543ProPhe: 1.543 ± 0.026	6.473ProGly: 6.473 ± 0.07	1.435ProHis: 1.435 ± 0.026	1.337ProIle: 1.337 ± 0.022	1.242ProLys: 1.242 ± 0.028	5.358ProLeu: 5.358 ± 0.056	0.987ProMet: 0.987 ± 0.022	0.957ProAsn: 0.957 ± 0.024	3.51ProPro: 3.51 ± 0.057	1.925ProGln: 1.925 ± 0.035	3.967ProArg: 3.967 ± 0.057	3.335ProSer: 3.335 ± 0.047	3.212ProThr: 3.212 ± 0.041	5.508ProVal: 5.508 ± 0.045	0.951ProTrp: 0.951 ± 0.019	1.49ProTyr: 1.49 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.862GlnAla: 3.862 ± 0.049	0.18GlnCys: 0.18 ± 0.008	1.499GlnAsp: 1.499 ± 0.025	1.53GlnGlu: 1.53 ± 0.027	0.711GlnPhe: 0.711 ± 0.017	2.456GlnGly: 2.456 ± 0.033	0.722GlnHis: 0.722 ± 0.017	1.129GlnIle: 1.129 ± 0.023	0.683GlnLys: 0.683 ± 0.02	3.048GlnLeu: 3.048 ± 0.037	0.534GlnMet: 0.534 ± 0.016	0.587GlnAsn: 0.587 ± 0.017	1.722GlnPro: 1.722 ± 0.032	1.325GlnGln: 1.325 ± 0.031	2.399GlnArg: 2.399 ± 0.033	1.362GlnSer: 1.362 ± 0.026	1.437GlnThr: 1.437 ± 0.027	2.488GlnVal: 2.488 ± 0.033	0.542GlnTrp: 0.542 ± 0.017	0.673GlnTyr: 0.673 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	9.514ArgAla: 9.514 ± 0.082	0.586ArgCys: 0.586 ± 0.017	4.131ArgAsp: 4.131 ± 0.044	4.661ArgGlu: 4.661 ± 0.062	2.298ArgPhe: 2.298 ± 0.034	5.715ArgGly: 5.715 ± 0.053	2.192ArgHis: 2.192 ± 0.035	3.218ArgIle: 3.218 ± 0.04	1.634ArgLys: 1.634 ± 0.026	8.874ArgLeu: 8.874 ± 0.085	1.697ArgMet: 1.697 ± 0.027	1.363ArgAsn: 1.363 ± 0.028	4.956ArgPro: 4.956 ± 0.062	2.395ArgGln: 2.395 ± 0.037	7.901ArgArg: 7.901 ± 0.09	4.034ArgSer: 4.034 ± 0.045	5.343ArgThr: 5.343 ± 0.049	5.803ArgVal: 5.803 ± 0.052	1.379ArgTrp: 1.379 ± 0.027	1.796ArgTyr: 1.796 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.678SerAla: 6.678 ± 0.057	0.436SerCys: 0.436 ± 0.014	2.75SerAsp: 2.75 ± 0.037	2.349SerGlu: 2.349 ± 0.034	1.591SerPhe: 1.591 ± 0.026	6.1SerGly: 6.1 ± 0.069	1.072SerHis: 1.072 ± 0.025	1.483SerIle: 1.483 ± 0.026	1.105SerLys: 1.105 ± 0.025	4.905SerLeu: 4.905 ± 0.044	1.103SerMet: 1.103 ± 0.028	0.928SerAsn: 0.928 ± 0.023	3.27SerPro: 3.27 ± 0.036	1.318SerGln: 1.318 ± 0.025	3.719SerArg: 3.719 ± 0.046	3.103SerSer: 3.103 ± 0.061	3.265SerThr: 3.265 ± 0.057	4.344SerVal: 4.344 ± 0.046	0.971SerTrp: 0.971 ± 0.021	1.293SerTyr: 1.293 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	8.989ThrAla: 8.989 ± 0.075	0.463ThrCys: 0.463 ± 0.014	3.714ThrAsp: 3.714 ± 0.055	3.169ThrGlu: 3.169 ± 0.05	1.681ThrPhe: 1.681 ± 0.028	6.799ThrGly: 6.799 ± 0.064	1.24ThrHis: 1.24 ± 0.023	1.781ThrIle: 1.781 ± 0.029	1.236ThrLys: 1.236 ± 0.027	5.785ThrLeu: 5.785 ± 0.05	0.95ThrMet: 0.95 ± 0.018	1.097ThrAsn: 1.097 ± 0.021	4.143ThrPro: 4.143 ± 0.049	1.442ThrGln: 1.442 ± 0.029	3.913ThrArg: 3.913 ± 0.044	3.374ThrSer: 3.374 ± 0.059	4.083ThrThr: 4.083 ± 0.078	6.248ThrVal: 6.248 ± 0.054	0.983ThrTrp: 0.983 ± 0.022	1.494ThrTyr: 1.494 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	10.612ValAla: 10.612 ± 0.089	0.775ValCys: 0.775 ± 0.02	4.881ValAsp: 4.881 ± 0.045	4.579ValGlu: 4.579 ± 0.048	2.507ValPhe: 2.507 ± 0.036	6.59ValGly: 6.59 ± 0.053	2.007ValHis: 2.007 ± 0.032	3.013ValIle: 3.013 ± 0.039	1.819ValLys: 1.819 ± 0.035	9.533ValLeu: 9.533 ± 0.081	1.446ValMet: 1.446 ± 0.024	1.724ValAsn: 1.724 ± 0.027	5.179ValPro: 5.179 ± 0.047	2.194ValGln: 2.194 ± 0.033	7.168ValArg: 7.168 ± 0.068	4.432ValSer: 4.432 ± 0.043	5.8ValThr: 5.8 ± 0.061	8.037ValVal: 8.037 ± 0.066	1.178ValTrp: 1.178 ± 0.021	1.68ValTyr: 1.68 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.719TrpAla: 1.719 ± 0.03	0.154TrpCys: 0.154 ± 0.008	0.849TrpAsp: 0.849 ± 0.021	0.74TrpGlu: 0.74 ± 0.02	0.548TrpPhe: 0.548 ± 0.015	1.125TrpGly: 1.125 ± 0.022	0.397TrpHis: 0.397 ± 0.013	0.597TrpIle: 0.597 ± 0.017	0.413TrpLys: 0.413 ± 0.014	1.757TrpLeu: 1.757 ± 0.03	0.294TrpMet: 0.294 ± 0.011	0.464TrpAsn: 0.464 ± 0.016	0.79TrpPro: 0.79 ± 0.017	0.662TrpGln: 0.662 ± 0.015	1.34TrpArg: 1.34 ± 0.026	0.989TrpSer: 0.989 ± 0.019	1.094TrpThr: 1.094 ± 0.025	1.022TrpVal: 1.022 ± 0.02	0.337TrpTrp: 0.337 ± 0.012	0.411TrpTyr: 0.411 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.877TyrAla: 2.877 ± 0.038	0.19TyrCys: 0.19 ± 0.009	1.667TyrAsp: 1.667 ± 0.038	1.291TyrGlu: 1.291 ± 0.023	0.713TyrPhe: 0.713 ± 0.02	2.408TyrGly: 2.408 ± 0.033	0.418TyrHis: 0.418 ± 0.012	0.558TyrIle: 0.558 ± 0.016	0.448TyrLys: 0.448 ± 0.016	2.132TyrLeu: 2.132 ± 0.035	0.277TyrMet: 0.277 ± 0.012	0.481TyrAsn: 0.481 ± 0.02	1.128TyrPro: 1.128 ± 0.022	0.671TyrGln: 0.671 ± 0.019	1.815TyrArg: 1.815 ± 0.034	1.044TyrSer: 1.044 ± 0.024	1.301TyrThr: 1.301 ± 0.027	1.75TyrVal: 1.75 ± 0.027	0.376TyrTrp: 0.376 ± 0.014	0.51TyrTyr: 0.51 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7307 proteins (2329859 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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