Aurantibacter aestuarii
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2594 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T1N5N3|A0A2T1N5N3_9FLAO Carboxymuconolactone decarboxylase family protein OS=Aurantibacter aestuarii OX=1266046 GN=C7H52_12820 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.49 LLNRR6 pKa = 11.84 FLGLAILLALFTTSCSSDD24 pKa = 3.21 DD25 pKa = 3.97 DD26 pKa = 4.98 GINVGQSPSVPLGAYY41 pKa = 9.19 EE42 pKa = 4.14 NGTFILNEE50 pKa = 4.08 GSSAATAGVDD60 pKa = 4.01 FLSDD64 pKa = 3.41 AGVLTSDD71 pKa = 3.9 VFRR74 pKa = 11.84 IEE76 pKa = 5.18 NPDD79 pKa = 3.45 EE80 pKa = 5.24 DD81 pKa = 4.3 EE82 pKa = 6.18 LGLFLQNIFFDD93 pKa = 3.75 DD94 pKa = 3.26 TRR96 pKa = 11.84 AFIISGGTSSITVVNRR112 pKa = 11.84 YY113 pKa = 5.55 TFKK116 pKa = 10.98 FITSIEE122 pKa = 4.32 TNLDD126 pKa = 3.19 NPRR129 pKa = 11.84 YY130 pKa = 9.84 GIVVNGKK137 pKa = 10.41 AYY139 pKa = 8.74 VTNQAGFSTGADD151 pKa = 3.16 DD152 pKa = 5.31 FVTVINLSNYY162 pKa = 7.18 STSTVLIGDD171 pKa = 3.76 YY172 pKa = 10.74 VDD174 pKa = 5.69 RR175 pKa = 11.84 ITFAGDD181 pKa = 3.02 KK182 pKa = 10.69 VVIANGAFGSGNGVTFLNTTNNSFQTLDD210 pKa = 3.63 LGAGNSPNSLIAVNDD225 pKa = 3.32 NVYY228 pKa = 11.28 ALTGNNKK235 pKa = 9.19 FFQIDD240 pKa = 3.62 ANTATLSSTLDD251 pKa = 3.02 IPAAISGVKK260 pKa = 9.92 NLQIEE265 pKa = 4.03 NDD267 pKa = 3.67 EE268 pKa = 4.69 VYY270 pKa = 8.9 FTADD274 pKa = 2.79 TSVYY278 pKa = 10.84 SFGLGDD284 pKa = 3.75 TTVSTTPLFTYY295 pKa = 10.67 NSTSQFGAMYY305 pKa = 10.08 GFSVSDD311 pKa = 3.28 NSIFVGDD318 pKa = 4.18 AGDD321 pKa = 3.53 FASAGTFFEE330 pKa = 4.73 YY331 pKa = 8.34 TTAGTLVDD339 pKa = 4.56 SYY341 pKa = 10.31 TSSGVGPNGFYY352 pKa = 10.98 KK353 pKa = 10.8 NN354 pKa = 3.55
Molecular weight: 37.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.678
IPC2_protein 3.719
IPC_protein 3.745
Toseland 3.503
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.719
Rodwell 3.567
Grimsley 3.414
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.151
Thurlkill 3.567
EMBOSS 3.719
Sillero 3.872
Patrickios 1.138
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A2T1N5H3|A0A2T1N5H3_9FLAO Adenylosuccinate lyase OS=Aurantibacter aestuarii OX=1266046 GN=C7H52_12505 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 7.97 KK42 pKa = 10.6 LSVSSEE48 pKa = 4.04 SRR50 pKa = 11.84 HH51 pKa = 5.63 KK52 pKa = 10.69 KK53 pKa = 9.55
Molecular weight: 6.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2594
0
2594
888837
38
3003
342.7
38.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.27 ± 0.042
0.76 ± 0.014
5.555 ± 0.04
6.385 ± 0.055
5.461 ± 0.042
6.059 ± 0.055
1.74 ± 0.024
7.9 ± 0.049
7.935 ± 0.078
9.453 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.947 ± 0.023
6.799 ± 0.058
3.237 ± 0.029
3.429 ± 0.025
3.066 ± 0.032
6.541 ± 0.038
6.246 ± 0.063
6.152 ± 0.033
0.934 ± 0.017
4.129 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here