Roseimicrobium gellanilyticum
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6509 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A366HRP9|A0A366HRP9_9BACT Uncharacterized protein OS=Roseimicrobium gellanilyticum OX=748857 GN=DES53_102313 PE=4 SV=1
MM1 pKa = 7.46 QILAVAAMSFSPLPSGMASWSDD23 pKa = 3.1 SDD25 pKa = 4.96 FNGSNDD31 pKa = 3.14 TWSDD35 pKa = 3.47 PLTSQVTTLADD46 pKa = 4.44 LNLQNNDD53 pKa = 3.64 ADD55 pKa = 4.5 NDD57 pKa = 4.04 GATNDD62 pKa = 3.79 EE63 pKa = 4.39 EE64 pKa = 4.98 ASEE67 pKa = 4.61 GSDD70 pKa = 3.0 PFSFDD75 pKa = 2.63 TDD77 pKa = 3.62 YY78 pKa = 11.87 DD79 pKa = 3.9 GLSDD83 pKa = 4.11 GDD85 pKa = 3.92 EE86 pKa = 4.05 LHH88 pKa = 6.71 LVKK91 pKa = 10.63 PSLAVSLTDD100 pKa = 3.16 WDD102 pKa = 3.89 TDD104 pKa = 3.27 NDD106 pKa = 3.89 EE107 pKa = 4.38 VSDD110 pKa = 3.81 YY111 pKa = 11.65 DD112 pKa = 3.32 EE113 pKa = 3.87 WHH115 pKa = 6.3 NFSGVTYY122 pKa = 10.13 PGGQLPSYY130 pKa = 9.69 PGASYY135 pKa = 11.25 SDD137 pKa = 3.77 YY138 pKa = 11.51 DD139 pKa = 3.72 GDD141 pKa = 4.11 GFKK144 pKa = 11.17 NPVDD148 pKa = 3.88 PQPTVPYY155 pKa = 10.5 DD156 pKa = 3.41 PTTTDD161 pKa = 2.87 SDD163 pKa = 3.71 SDD165 pKa = 3.46 NWYY168 pKa = 10.03 DD169 pKa = 4.82 AYY171 pKa = 11.03 DD172 pKa = 4.32 PFPNDD177 pKa = 3.37 PTNFSPINGIAWGQTFHH194 pKa = 7.96 DD195 pKa = 4.86 DD196 pKa = 3.94 QDD198 pKa = 4.56 GDD200 pKa = 5.03 LLLNWEE206 pKa = 4.77 DD207 pKa = 3.68 PVPYY211 pKa = 10.16 GPSDD215 pKa = 3.63 SDD217 pKa = 3.71 SDD219 pKa = 4.5 GITDD223 pKa = 4.73 GEE225 pKa = 4.5 DD226 pKa = 3.65 PYY228 pKa = 11.24 PIDD231 pKa = 4.55 PYY233 pKa = 11.27 NYY235 pKa = 9.43 SQINHH240 pKa = 6.77 IYY242 pKa = 7.94 WHH244 pKa = 6.43 GEE246 pKa = 3.56 VRR248 pKa = 11.84 GDD250 pKa = 3.58 SDD252 pKa = 5.41 NDD254 pKa = 3.53 GTLNWEE260 pKa = 4.49 DD261 pKa = 3.9 PQPYY265 pKa = 9.7 GPPDD269 pKa = 3.75 SDD271 pKa = 3.51 SDD273 pKa = 4.43 GIIDD277 pKa = 4.84 EE278 pKa = 4.43 EE279 pKa = 4.65 DD280 pKa = 4.54 PYY282 pKa = 11.36 PSDD285 pKa = 3.55 PNNYY289 pKa = 9.34 SEE291 pKa = 4.31 TNYY294 pKa = 8.33 VHH296 pKa = 7.38 WYY298 pKa = 10.67 AEE300 pKa = 4.05 VLGDD304 pKa = 4.32 SDD306 pKa = 5.99 NDD308 pKa = 3.95 GTPNWQDD315 pKa = 3.39 PQPYY319 pKa = 9.73 GPPANDD325 pKa = 3.4 ADD327 pKa = 4.27 ADD329 pKa = 4.03 GFANDD334 pKa = 3.86 IDD336 pKa = 4.29 PFAFDD341 pKa = 3.79 ASNYY345 pKa = 9.74 SAINNTNWYY354 pKa = 10.19 ADD356 pKa = 3.48 VLGDD360 pKa = 4.1 IDD362 pKa = 5.8 SDD364 pKa = 4.89 QILNWTDD371 pKa = 3.47 PFPNDD376 pKa = 3.13 STNYY380 pKa = 9.73 SAINSTSWHH389 pKa = 6.53 ADD391 pKa = 3.13 VLGDD395 pKa = 4.38 ADD397 pKa = 5.6 SDD399 pKa = 4.74 TIQNWQDD406 pKa = 3.02 GAPYY410 pKa = 10.59 GDD412 pKa = 4.44 TPPPPDD418 pKa = 5.05 PDD420 pKa = 3.7 SDD422 pKa = 4.14 GDD424 pKa = 4.0 GLTEE428 pKa = 4.13 STEE431 pKa = 4.05 AAYY434 pKa = 8.15 GTSDD438 pKa = 4.68 SDD440 pKa = 4.37 VDD442 pKa = 4.43 CDD444 pKa = 5.24 DD445 pKa = 6.25 DD446 pKa = 4.94 GLTDD450 pKa = 3.65 HH451 pKa = 7.31 EE452 pKa = 4.79 EE453 pKa = 3.76 LVIYY457 pKa = 7.3 YY458 pKa = 7.33 TNPLNMYY465 pKa = 9.56 HH466 pKa = 7.55 LSQTAGWGDD475 pKa = 4.84 LYY477 pKa = 10.9 TDD479 pKa = 3.85 YY480 pKa = 11.8 QMVDD484 pKa = 3.1 PTDD487 pKa = 3.8 FDD489 pKa = 4.56 GDD491 pKa = 4.49 SIPDD495 pKa = 4.15 RR496 pKa = 11.84 IEE498 pKa = 3.42 LHH500 pKa = 6.53 YY501 pKa = 11.47 GMLPNDD507 pKa = 4.02 AADD510 pKa = 3.6 AAGDD514 pKa = 3.85 LDD516 pKa = 4.68 GNGISNSAQYY526 pKa = 10.96 AAGRR530 pKa = 11.84 SLLADD535 pKa = 3.07 ILVYY539 pKa = 10.97 DD540 pKa = 4.24 GDD542 pKa = 4.49 GDD544 pKa = 3.91 GMTDD548 pKa = 3.14 VFEE551 pKa = 6.09 DD552 pKa = 3.29 AHH554 pKa = 6.03 QLNKK558 pKa = 10.82 NYY560 pKa = 10.39 FGDD563 pKa = 3.73 AMADD567 pKa = 3.22 EE568 pKa = 5.41 DD569 pKa = 4.11 MDD571 pKa = 4.07 GVFNFEE577 pKa = 3.95 EE578 pKa = 4.96 SKK580 pKa = 10.97 LQLNPRR586 pKa = 11.84 IPDD589 pKa = 3.17 THH591 pKa = 7.3 ARR593 pKa = 11.84 GGLGDD598 pKa = 4.69 LGVLMEE604 pKa = 4.61 AMLYY608 pKa = 10.13 PEE610 pKa = 5.51 GGAPTVDD617 pKa = 3.77 GDD619 pKa = 4.72 SDD621 pKa = 5.7 GIPDD625 pKa = 3.64 WQEE628 pKa = 3.54 VSLTAASPVFTRR640 pKa = 11.84 LAPNDD645 pKa = 3.54 MDD647 pKa = 4.94 GDD649 pKa = 4.33 SMPDD653 pKa = 2.9 IWEE656 pKa = 4.09 HH657 pKa = 6.17 QYY659 pKa = 11.27 GRR661 pKa = 11.84 WKK663 pKa = 10.9 YY664 pKa = 7.51 PTNGLYY670 pKa = 10.02 VRR672 pKa = 11.84 HH673 pKa = 6.74 HH674 pKa = 7.36 DD675 pKa = 3.49 GAGDD679 pKa = 3.62 IEE681 pKa = 5.63 GDD683 pKa = 3.62 GLSNYY688 pKa = 9.33 HH689 pKa = 6.66 EE690 pKa = 4.5 YY691 pKa = 10.66 WLMCDD696 pKa = 3.97 PMINDD701 pKa = 4.53 GGADD705 pKa = 3.72 PDD707 pKa = 4.3 GDD709 pKa = 3.94 GLGYY713 pKa = 10.44 AAEE716 pKa = 4.44 LALGTHH722 pKa = 6.7 LWVADD727 pKa = 3.41 TDD729 pKa = 3.57 MDD731 pKa = 4.95 GISDD735 pKa = 4.05 GEE737 pKa = 4.01 EE738 pKa = 4.31 DD739 pKa = 3.98 FDD741 pKa = 6.74 GDD743 pKa = 3.77 GLSNITEE750 pKa = 4.2 RR751 pKa = 11.84 SLSTNALNKK760 pKa = 9.83 DD761 pKa = 3.14 TDD763 pKa = 3.79 ADD765 pKa = 3.54 GWDD768 pKa = 3.99 DD769 pKa = 5.57 DD770 pKa = 4.89 EE771 pKa = 4.48 EE772 pKa = 4.4 QAMGSNPLSPEE783 pKa = 4.04 DD784 pKa = 3.86 QPILEE789 pKa = 4.62 PMSSYY794 pKa = 11.39 VSIQTRR800 pKa = 11.84 WVHH803 pKa = 6.58 SSWQEE808 pKa = 3.65 HH809 pKa = 5.16 GGEE812 pKa = 4.25 YY813 pKa = 10.17 YY814 pKa = 10.48 SRR816 pKa = 11.84 YY817 pKa = 9.12 WYY819 pKa = 6.92 WTWDD823 pKa = 3.58 SVDD826 pKa = 3.57 PPMGHH831 pKa = 6.61 WEE833 pKa = 3.99 EE834 pKa = 4.28 QFYY837 pKa = 9.81 STTTPRR843 pKa = 11.84 DD844 pKa = 3.08 EE845 pKa = 5.01 AGQANFSDD853 pKa = 4.55 GTSQPISTFSAAQGALGQVSQMDD876 pKa = 3.46 WQEE879 pKa = 3.83 SGEE882 pKa = 4.18 RR883 pKa = 11.84 SVSSSWTEE891 pKa = 3.33 ITGRR895 pKa = 11.84 PMPEE899 pKa = 3.58 PPQYY903 pKa = 10.74 FFYY906 pKa = 10.38 PDD908 pKa = 3.46 GGEE911 pKa = 4.25 EE912 pKa = 4.22 SPGWSGYY919 pKa = 8.57 TEE921 pKa = 4.72 DD922 pKa = 3.59 NRR924 pKa = 11.84 VWSGGSLEE932 pKa = 5.49 VRR934 pKa = 11.84 LAWKK938 pKa = 9.86 PNAPKK943 pKa = 9.71 PHH945 pKa = 6.7 RR946 pKa = 11.84 RR947 pKa = 11.84 HH948 pKa = 6.48 DD949 pKa = 3.61 LTVTFMEE956 pKa = 4.5 TEE958 pKa = 4.41 TVNEE962 pKa = 4.3 AEE964 pKa = 4.23 PDD966 pKa = 3.44 ITFHH970 pKa = 6.89 ALKK973 pKa = 10.36 ILANQQFSSAGGTNGGAINLVANASTPEE1001 pKa = 4.09 TTVVKK1006 pKa = 10.69 SLVQNAEE1013 pKa = 3.19 IRR1015 pKa = 11.84 TYY1017 pKa = 10.95 DD1018 pKa = 4.11 YY1019 pKa = 10.69 YY1020 pKa = 10.65 WKK1022 pKa = 10.35 NQGSATNPRR1031 pKa = 11.84 PDD1033 pKa = 3.71 DD1034 pKa = 3.59 NVRR1037 pKa = 11.84 THH1039 pKa = 6.53 YY1040 pKa = 10.0 PDD1042 pKa = 3.41 SSSPTGYY1049 pKa = 8.37 RR1050 pKa = 11.84 TKK1052 pKa = 10.15 KK1053 pKa = 8.36 TDD1055 pKa = 3.27 KK1056 pKa = 10.99 LKK1058 pKa = 10.06 IAQWYY1063 pKa = 9.27 DD1064 pKa = 3.31 VVNGGVFHH1072 pKa = 7.04 PNKK1075 pKa = 10.16 IEE1077 pKa = 4.4 ADD1079 pKa = 3.13 TDD1081 pKa = 3.6 AFFIRR1086 pKa = 11.84 LTNPPQLPSGTTYY1099 pKa = 10.34 RR1100 pKa = 11.84 AKK1102 pKa = 10.48 VWTEE1106 pKa = 3.62 NASGAVVDD1114 pKa = 4.5 SGADD1118 pKa = 3.36 VEE1120 pKa = 4.56 LQPVANQPGTYY1131 pKa = 8.93 QSKK1134 pKa = 10.17 GLCLVSNSYY1143 pKa = 11.59 DD1144 pKa = 3.47 DD1145 pKa = 4.47 AQATVSGPDD1154 pKa = 3.18 NGLNDD1159 pKa = 3.33 QSYY1162 pKa = 8.95 NAEE1165 pKa = 4.06 LGGSVKK1171 pKa = 9.96 IRR1173 pKa = 11.84 FLFGTNPTATTVDD1186 pKa = 3.38 MVLPVPVKK1194 pKa = 9.92 KK1195 pKa = 9.0 TLTVQGYY1202 pKa = 8.04 ILKK1205 pKa = 10.31 SGTISPSDD1213 pKa = 3.84 VIAPAAANTYY1223 pKa = 10.79 LEE1225 pKa = 4.21 LAKK1228 pKa = 10.6 EE1229 pKa = 4.08 RR1230 pKa = 11.84 LAQVGVEE1237 pKa = 3.95 LDD1239 pKa = 3.74 GTISTVTANPSGVSFGGFFPAKK1261 pKa = 10.09 FEE1263 pKa = 4.27 EE1264 pKa = 4.27 PHH1266 pKa = 6.22 GASSLTIPPEE1276 pKa = 4.05 SVALFTALNPQVDD1289 pKa = 3.85 VTFYY1293 pKa = 10.73 FVSDD1297 pKa = 3.96 FANSSNQGMTVVPKK1311 pKa = 8.55 TTSLTAFHH1319 pKa = 6.52 NRR1321 pKa = 11.84 ILIDD1325 pKa = 3.82 VPAMADD1331 pKa = 3.09 DD1332 pKa = 4.02 TLAHH1336 pKa = 6.47 EE1337 pKa = 4.62 FLHH1340 pKa = 7.42 VLMNAVHH1347 pKa = 6.95 GPPFNDD1353 pKa = 3.92 FLRR1356 pKa = 11.84 EE1357 pKa = 4.06 HH1358 pKa = 6.9 GSDD1361 pKa = 5.37 DD1362 pKa = 3.88 NNLWHH1367 pKa = 7.15 HH1368 pKa = 6.7 NDD1370 pKa = 3.45 GAIGDD1375 pKa = 3.77 IFKK1378 pKa = 9.64 RR1379 pKa = 11.84 TRR1381 pKa = 11.84 INDD1384 pKa = 2.86 AMYY1387 pKa = 10.23 YY1388 pKa = 10.61 KK1389 pKa = 10.07 MYY1391 pKa = 7.55 PTPPP1395 pKa = 3.43
Molecular weight: 152.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.694
IPC_protein 3.77
Toseland 3.528
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.579
Grimsley 3.427
Solomon 3.77
Lehninger 3.719
Nozaki 3.872
DTASelect 4.164
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.884
Patrickios 0.909
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.792
Protein with the highest isoelectric point:
>tr|A0A366H6Z0|A0A366H6Z0_9BACT Uncharacterized protein OS=Roseimicrobium gellanilyticum OX=748857 GN=DES53_1143 PE=4 SV=1
MM1 pKa = 7.18 NAIVYY6 pKa = 10.0 VNTPLEE12 pKa = 4.49 APEE15 pKa = 4.2 HH16 pKa = 5.86 AALAALARR24 pKa = 11.84 RR25 pKa = 11.84 EE26 pKa = 4.0 GRR28 pKa = 11.84 AKK30 pKa = 10.38 GQQLRR35 pKa = 11.84 LLAIQAMRR43 pKa = 11.84 DD44 pKa = 3.48 RR45 pKa = 11.84 NQGRR49 pKa = 11.84 SKK51 pKa = 10.61 SRR53 pKa = 11.84 SRR55 pKa = 11.84 GIMPAGSDD63 pKa = 3.56 SYY65 pKa = 11.44 VPHH68 pKa = 7.19 APAPLPSCSCSSSPCSCSCSDD89 pKa = 3.82 AGAAGSPKK97 pKa = 10.12 VQLATASDD105 pKa = 3.37 SRR107 pKa = 11.84 TRR109 pKa = 11.84 RR110 pKa = 11.84 STSTNTNTNTSPCTGTGTDD129 pKa = 4.55 ARR131 pKa = 11.84 AALQKK136 pKa = 10.56 GGRR139 pKa = 11.84 ARR141 pKa = 11.84 GG142 pKa = 3.38
Molecular weight: 14.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.385
IPC_protein 10.043
Toseland 10.409
ProMoST 10.116
Dawson 10.54
Bjellqvist 10.306
Wikipedia 10.73
Rodwell 10.672
Grimsley 10.584
Solomon 10.643
Lehninger 10.628
Nozaki 10.511
DTASelect 10.248
Thurlkill 10.438
EMBOSS 10.818
Sillero 10.496
Patrickios 10.452
IPC_peptide 10.657
IPC2_peptide 9.809
IPC2.peptide.svr19 8.379
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6509
0
6509
2350827
29
10978
361.2
39.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.239 ± 0.034
0.993 ± 0.012
5.251 ± 0.029
6.183 ± 0.047
3.786 ± 0.018
8.124 ± 0.071
2.377 ± 0.018
4.499 ± 0.023
4.689 ± 0.043
9.908 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.424 ± 0.02
3.142 ± 0.05
5.521 ± 0.037
3.527 ± 0.021
6.124 ± 0.041
6.06 ± 0.03
5.886 ± 0.067
7.155 ± 0.029
1.666 ± 0.018
2.447 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here