Baumannia cicadellinicola subsp. Homalodisca coagulata
Average proteome isoelectric point is 7.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 595 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q1LT37|FABH_BAUCH 3-oxoacyl-[acyl-carrier-protein] synthase 3 OS=Baumannia cicadellinicola subsp. Homalodisca coagulata OX=374463 GN=fabH PE=3 SV=1
MM1 pKa = 7.92 LITINEE7 pKa = 4.2 CLLLFTLTYY16 pKa = 10.85 IMLCIDD22 pKa = 3.52 ISLFLISFIFTT33 pKa = 3.61
Molecular weight: 3.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.192
IPC2_protein 4.101
IPC_protein 3.287
Toseland 3.312
ProMoST 3.732
Dawson 3.427
Bjellqvist 3.668
Wikipedia 3.567
Rodwell 3.3
Grimsley 3.3
Solomon 3.058
Lehninger 3.007
Nozaki 3.745
DTASelect 3.567
Thurlkill 3.528
EMBOSS 3.554
Sillero 3.503
Patrickios 0.006
IPC_peptide 3.045
IPC2_peptide 3.389
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|Q1LTX4|Q1LTX4_BAUCH N5-carboxyaminoimidazole ribonucleotide synthase OS=Baumannia cicadellinicola subsp. Homalodisca coagulata OX=374463 GN=purK PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.58 RR3 pKa = 11.84 TFQPSLLKK11 pKa = 10.25 RR12 pKa = 11.84 HH13 pKa = 5.45 RR14 pKa = 11.84 THH16 pKa = 7.36 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 IATKK25 pKa = 10.16 SGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.07 KK38 pKa = 10.04 RR39 pKa = 11.84 SRR41 pKa = 11.84 LTISNRR47 pKa = 3.19
Molecular weight: 5.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
595
0
595
194890
31
1408
327.5
36.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.982 ± 0.092
1.322 ± 0.035
4.436 ± 0.058
5.03 ± 0.091
3.732 ± 0.065
6.102 ± 0.083
2.554 ± 0.04
9.89 ± 0.09
5.908 ± 0.07
10.902 ± 0.108
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.475 ± 0.037
5.688 ± 0.078
3.644 ± 0.048
4.48 ± 0.062
4.985 ± 0.079
6.082 ± 0.051
5.297 ± 0.051
5.842 ± 0.077
1.099 ± 0.04
3.545 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here