Rhodococcus phage REQ2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9FH10|G9FH10_9CAUD Putative membrane protein OS=Rhodococcus phage REQ2 OX=1109713 PE=4 SV=1
MM1 pKa = 6.96STAEE5 pKa = 3.93QIIAEE10 pKa = 4.5HH11 pKa = 6.44RR12 pKa = 11.84WSALTDD18 pKa = 3.22ACKK21 pKa = 10.79CGDD24 pKa = 3.5PTITLGEE31 pKa = 4.14HH32 pKa = 6.17AAHH35 pKa = 6.1VVAALTNAGKK45 pKa = 10.4AIVEE49 pKa = 4.14LPEE52 pKa = 6.02AEE54 pKa = 5.12DD55 pKa = 4.23YY56 pKa = 11.63GGDD59 pKa = 3.92PQWTLPCGTVSVDD72 pKa = 2.89SRR74 pKa = 11.84QVVSFEE80 pKa = 4.6GDD82 pKa = 3.92DD83 pKa = 3.32ICDD86 pKa = 3.58ANEE89 pKa = 4.29APDD92 pKa = 3.74LAAALLAAARR102 pKa = 11.84VAEE105 pKa = 4.76GGEE108 pKa = 4.13QPP110 pKa = 4.51

Molecular weight: 11.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9FH03|G9FH03_9CAUD Putative membrane protein OS=Rhodococcus phage REQ2 OX=1109713 PE=4 SV=1
MM1 pKa = 7.27TKK3 pKa = 10.47YY4 pKa = 10.37PVTTVPKK11 pKa = 9.94LASEE15 pKa = 4.3KK16 pKa = 10.02ALKK19 pKa = 8.92HH20 pKa = 5.77RR21 pKa = 11.84RR22 pKa = 11.84MKK24 pKa = 10.34FAQAGVPMLVEE35 pKa = 4.06SAPIRR40 pKa = 11.84AHH42 pKa = 6.64IDD44 pKa = 3.27EE45 pKa = 4.84LLGIGMSAMMIARR58 pKa = 11.84RR59 pKa = 11.84AGVGDD64 pKa = 3.24RR65 pKa = 11.84TIRR68 pKa = 11.84KK69 pKa = 8.61IRR71 pKa = 11.84SGEE74 pKa = 3.88YY75 pKa = 10.37AKK77 pKa = 9.45TQFLVAQRR85 pKa = 11.84IMAADD90 pKa = 4.12HH91 pKa = 6.21MPAPHH96 pKa = 6.8MSFVMPVGAVRR107 pKa = 11.84RR108 pKa = 11.84VRR110 pKa = 11.84ALQAFGWTLTDD121 pKa = 2.84IGSRR125 pKa = 11.84IGMADD130 pKa = 3.33TNIAALQHH138 pKa = 4.97QRR140 pKa = 11.84RR141 pKa = 11.84VSYY144 pKa = 9.66SLWVRR149 pKa = 11.84VRR151 pKa = 11.84DD152 pKa = 3.9VYY154 pKa = 11.34EE155 pKa = 4.35EE156 pKa = 4.39LSSTPGPSGDD166 pKa = 3.47SRR168 pKa = 11.84HH169 pKa = 5.35RR170 pKa = 11.84AARR173 pKa = 11.84KK174 pKa = 8.44GWLNPFEE181 pKa = 4.32WEE183 pKa = 5.18GYY185 pKa = 10.52DD186 pKa = 3.62IDD188 pKa = 4.72DD189 pKa = 4.71PRR191 pKa = 11.84VTPPRR196 pKa = 11.84SARR199 pKa = 11.84TTADD203 pKa = 3.16RR204 pKa = 11.84SGARR208 pKa = 11.84ADD210 pKa = 4.01RR211 pKa = 11.84LEE213 pKa = 4.02QVADD217 pKa = 3.93LTAQGLSAGAIADD230 pKa = 3.52QLGVSEE236 pKa = 4.7RR237 pKa = 11.84QIQRR241 pKa = 11.84DD242 pKa = 3.22RR243 pKa = 11.84SAAA246 pKa = 3.44

Molecular weight: 27.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

15227

38

1937

185.7

20.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.043 ± 0.716

0.827 ± 0.147

6.62 ± 0.269

5.74 ± 0.254

2.607 ± 0.201

8.111 ± 0.426

2.207 ± 0.226

4.912 ± 0.203

3.435 ± 0.247

8.019 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.226 ± 0.102

2.824 ± 0.146

5.136 ± 0.286

3.448 ± 0.136

7.29 ± 0.38

5.608 ± 0.273

6.83 ± 0.312

7.086 ± 0.27

2.056 ± 0.141

1.977 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski