Mycobacterium phage Oline

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Pegunavirus; Mycobacterium virus Oline

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1FFS5|G1FFS5_9CAUD Crossover junction endodeoxyribonuclease RuvC OS=Mycobacterium phage Oline OX=1052671 GN=6 PE=3 SV=1
MM1 pKa = 8.05ADD3 pKa = 2.86QDD5 pKa = 4.35FYY7 pKa = 11.87FEE9 pKa = 4.3VDD11 pKa = 3.13GQRR14 pKa = 11.84FDD16 pKa = 3.68QRR18 pKa = 11.84VEE20 pKa = 4.12AMAHH24 pKa = 5.96CDD26 pKa = 3.1LYY28 pKa = 10.97GYY30 pKa = 10.06HH31 pKa = 6.61YY32 pKa = 11.03SAIQWVDD39 pKa = 3.74PNAPWAA45 pKa = 4.04

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1FFZ5|G1FFZ5_9CAUD Uncharacterized protein OS=Mycobacterium phage Oline OX=1052671 GN=76 PE=4 SV=1
MM1 pKa = 7.48ARR3 pKa = 11.84ATRR6 pKa = 11.84ARR8 pKa = 11.84GTRR11 pKa = 11.84SRR13 pKa = 11.84LGRR16 pKa = 11.84GSARR20 pKa = 11.84IARR23 pKa = 11.84SRR25 pKa = 11.84QRR27 pKa = 11.84GTISMSAATGGVGQFAKK44 pKa = 10.51RR45 pKa = 11.84GGRR48 pKa = 11.84RR49 pKa = 11.84GGGYY53 pKa = 10.09RR54 pKa = 11.84GFKK57 pKa = 10.23SKK59 pKa = 10.67KK60 pKa = 5.54QWRR63 pKa = 11.84WAWATKK69 pKa = 9.83QPWARR74 pKa = 11.84KK75 pKa = 8.71KK76 pKa = 10.09AHH78 pKa = 5.19EE79 pKa = 4.31TKK81 pKa = 9.92GGKK84 pKa = 7.0VVRR87 pKa = 11.84YY88 pKa = 9.47RR89 pKa = 11.84RR90 pKa = 11.84LPEE93 pKa = 4.35SKK95 pKa = 10.31HH96 pKa = 5.36SGHH99 pKa = 7.0RR100 pKa = 11.84GRR102 pKa = 11.84RR103 pKa = 11.84GPSRR107 pKa = 11.84KK108 pKa = 9.63GG109 pKa = 3.11

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

21692

36

1992

214.8

23.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.175 ± 0.391

1.171 ± 0.157

6.468 ± 0.293

5.938 ± 0.34

2.314 ± 0.114

9.676 ± 0.655

2.084 ± 0.206

4.661 ± 0.153

2.955 ± 0.172

7.883 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.107

2.914 ± 0.212

6.058 ± 0.28

3.047 ± 0.136

6.767 ± 0.306

4.983 ± 0.222

7.063 ± 0.222

7.51 ± 0.197

1.798 ± 0.139

2.448 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski