Sphingomonas sp. Leaf231

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3320 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S9C8U3|A0A0S9C8U3_9SPHN Uncharacterized protein OS=Sphingomonas sp. Leaf231 OX=1736301 GN=ASE95_14965 PE=4 SV=1
MM1 pKa = 7.18YY2 pKa = 10.3CDD4 pKa = 5.27DD5 pKa = 5.28GNDD8 pKa = 3.69TLFGEE13 pKa = 4.8AGNDD17 pKa = 3.69FLDD20 pKa = 4.48GGAGADD26 pKa = 4.07RR27 pKa = 11.84LWGDD31 pKa = 4.02RR32 pKa = 11.84GNDD35 pKa = 2.99QYY37 pKa = 11.76QYY39 pKa = 11.39GGIGKK44 pKa = 8.63HH45 pKa = 5.79VINDD49 pKa = 3.81GVDD52 pKa = 3.22QAGGARR58 pKa = 11.84TEE60 pKa = 4.3TTYY63 pKa = 9.97DD64 pKa = 3.26TSDD67 pKa = 3.32VLILTGAAYY76 pKa = 9.01TSQQITYY83 pKa = 10.15SRR85 pKa = 11.84VGNDD89 pKa = 3.86LLFTTAADD97 pKa = 3.66QTDD100 pKa = 3.91GVIDD104 pKa = 3.76NSVTITGFFNKK115 pKa = 8.88GHH117 pKa = 6.46

Molecular weight: 12.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S9CIM2|A0A0S9CIM2_9SPHN Hpr_kinase_C domain-containing protein OS=Sphingomonas sp. Leaf231 OX=1736301 GN=ASE95_00790 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3320

0

3320

1087510

30

2264

327.6

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.402 ± 0.067

0.694 ± 0.011

6.177 ± 0.032

4.819 ± 0.035

3.368 ± 0.024

9.116 ± 0.042

1.975 ± 0.02

4.567 ± 0.027

2.371 ± 0.027

9.709 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.312 ± 0.02

2.378 ± 0.033

5.336 ± 0.035

2.973 ± 0.028

8.032 ± 0.04

4.758 ± 0.033

5.718 ± 0.029

7.701 ± 0.033

1.44 ± 0.02

2.153 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski