Miniopterus schreibersii papillomavirus 1
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J9R1I3|J9R1I3_9PAPI Major capsid protein L1 OS=Miniopterus schreibersii papillomavirus 1 OX=1195364 GN=L1 PE=3 SV=1
MM1 pKa = 7.71 LYY3 pKa = 10.96 DD4 pKa = 4.93 NIPGYY9 pKa = 6.59 TTLDD13 pKa = 3.59 FVEE16 pKa = 4.78 SVQAAATTNSQGQLVVLSPCSIGASCCCCGYY47 pKa = 10.73 VVVLHH52 pKa = 6.6 FVATEE57 pKa = 3.74 DD58 pKa = 3.61 AAIVVDD64 pKa = 4.3 NLITTARR71 pKa = 11.84 LNLFCTRR78 pKa = 11.84 CVADD82 pKa = 4.45 LKK84 pKa = 10.45 RR85 pKa = 11.84 ARR87 pKa = 11.84 DD88 pKa = 3.72 GRR90 pKa = 11.84 QQ91 pKa = 2.65
Molecular weight: 9.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.018
IPC2_protein 5.143
IPC_protein 4.838
Toseland 4.685
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.991
Wikipedia 4.8
Rodwell 4.711
Grimsley 4.596
Solomon 4.838
Lehninger 4.8
Nozaki 4.978
DTASelect 5.219
Thurlkill 4.749
EMBOSS 4.825
Sillero 5.003
Patrickios 0.426
IPC_peptide 4.838
IPC2_peptide 4.978
IPC2.peptide.svr19 4.948
Protein with the highest isoelectric point:
>tr|J9QY83|J9QY83_9PAPI Replication protein E1 OS=Miniopterus schreibersii papillomavirus 1 OX=1195364 GN=E1 PE=3 SV=1
MM1 pKa = 7.08 NQRR4 pKa = 11.84 TDD6 pKa = 3.31 PLTARR11 pKa = 11.84 LEE13 pKa = 4.37 SVQEE17 pKa = 4.17 EE18 pKa = 4.11 IFEE21 pKa = 5.05 LIEE24 pKa = 4.06 EE25 pKa = 4.33 SSNRR29 pKa = 11.84 LEE31 pKa = 3.98 DD32 pKa = 3.64 QIRR35 pKa = 11.84 YY36 pKa = 8.43 WEE38 pKa = 4.87 LIRR41 pKa = 11.84 KK42 pKa = 7.27 EE43 pKa = 3.87 QALLFLARR51 pKa = 11.84 RR52 pKa = 11.84 EE53 pKa = 4.02 RR54 pKa = 11.84 ITRR57 pKa = 11.84 IGAEE61 pKa = 4.22 VVPPLSVSEE70 pKa = 4.29 TRR72 pKa = 11.84 AKK74 pKa = 10.35 QAIQMSLLLTSLNKK88 pKa = 9.89 SHH90 pKa = 6.71 YY91 pKa = 10.21 KK92 pKa = 9.87 NEE94 pKa = 3.83 PWTMQEE100 pKa = 3.78 TSRR103 pKa = 11.84 EE104 pKa = 3.88 RR105 pKa = 11.84 MLAPPKK111 pKa = 9.72 YY112 pKa = 8.11 TFKK115 pKa = 11.1 KK116 pKa = 9.82 AGKK119 pKa = 9.28 SVDD122 pKa = 3.46 IIFDD126 pKa = 3.88 GNIEE130 pKa = 4.04 NSVRR134 pKa = 11.84 EE135 pKa = 4.24 TMWGFIYY142 pKa = 10.89 YY143 pKa = 9.65 QDD145 pKa = 5.83 SDD147 pKa = 5.61 DD148 pKa = 4.07 EE149 pKa = 4.43 WQKK152 pKa = 10.98 QPGEE156 pKa = 4.17 IDD158 pKa = 3.63 DD159 pKa = 3.68 QGLFYY164 pKa = 10.56 RR165 pKa = 11.84 DD166 pKa = 3.2 YY167 pKa = 11.27 DD168 pKa = 3.68 NEE170 pKa = 4.12 KK171 pKa = 10.1 IYY173 pKa = 11.21 YY174 pKa = 9.98 VDD176 pKa = 4.28 FKK178 pKa = 11.2 EE179 pKa = 4.96 LAAKK183 pKa = 9.91 YY184 pKa = 9.93 SKK186 pKa = 10.19 EE187 pKa = 3.52 GRR189 pKa = 11.84 YY190 pKa = 9.41 KK191 pKa = 10.97 VIVDD195 pKa = 4.07 SKK197 pKa = 8.67 TVADD201 pKa = 3.88 VVVSHH206 pKa = 7.19 LDD208 pKa = 3.35 NSEE211 pKa = 4.13 SPVRR215 pKa = 11.84 TKK217 pKa = 10.6 AAPAGRR223 pKa = 11.84 RR224 pKa = 11.84 RR225 pKa = 11.84 ISGGRR230 pKa = 11.84 RR231 pKa = 11.84 RR232 pKa = 11.84 PRR234 pKa = 11.84 GRR236 pKa = 11.84 SSSHH240 pKa = 5.24 STPASTSSSSTPTRR254 pKa = 11.84 SRR256 pKa = 11.84 SRR258 pKa = 11.84 PRR260 pKa = 11.84 RR261 pKa = 11.84 AYY263 pKa = 9.25 TGAAPSPRR271 pKa = 11.84 EE272 pKa = 3.81 VGTQHH277 pKa = 7.19 ASVTGPSGSRR287 pKa = 11.84 LRR289 pKa = 11.84 RR290 pKa = 11.84 LLQEE294 pKa = 4.59 ARR296 pKa = 11.84 DD297 pKa = 3.98 PPAVILSGPANTVKK311 pKa = 10.63 CLRR314 pKa = 11.84 FRR316 pKa = 11.84 CRR318 pKa = 11.84 HH319 pKa = 5.99 RR320 pKa = 11.84 YY321 pKa = 9.02 NEE323 pKa = 4.03 YY324 pKa = 9.98 FDD326 pKa = 5.63 KK327 pKa = 10.92 ISTTWWWTGDD337 pKa = 2.81 GRR339 pKa = 11.84 TRR341 pKa = 11.84 TGDD344 pKa = 2.93 ASMLVSFQSEE354 pKa = 4.24 SQRR357 pKa = 11.84 TSFLNTVPVPSSVTVSAANLFLRR380 pKa = 4.84
Molecular weight: 43.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.943
IPC2_protein 9.136
IPC_protein 9.253
Toseland 9.736
ProMoST 9.604
Dawson 10.014
Bjellqvist 9.721
Wikipedia 10.218
Rodwell 10.204
Grimsley 10.101
Solomon 10.043
Lehninger 9.999
Nozaki 9.75
DTASelect 9.721
Thurlkill 9.838
EMBOSS 10.16
Sillero 9.926
Patrickios 5.55
IPC_peptide 10.028
IPC2_peptide 8.375
IPC2.peptide.svr19 8.188
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2412
91
669
344.6
38.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.965 ± 0.364
1.866 ± 0.711
5.846 ± 0.488
6.551 ± 0.505
4.063 ± 0.315
6.385 ± 0.799
2.612 ± 0.425
4.685 ± 0.331
4.809 ± 0.747
8.831 ± 0.821
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.824 ± 0.232
3.483 ± 0.405
6.302 ± 0.806
4.063 ± 0.24
7.67 ± 1.049
7.09 ± 0.902
6.965 ± 0.491
5.97 ± 0.778
1.119 ± 0.285
2.902 ± 0.448
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here