Bos grunniens papillomavirus 1
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7K4X5|J7K4X5_BPV1 Replication protein E1 OS=Bos grunniens papillomavirus 1 OX=2758380 GN=E1 PE=3 SV=1
MM1 pKa = 7.49 PNLWFLLFLGLVAAMQLLLLLFLLLFFLVYY31 pKa = 9.25 WDD33 pKa = 4.88 HH34 pKa = 6.96 FEE36 pKa = 4.44 CSCTSLPFF44 pKa = 4.63
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.503
IPC2_protein 4.876
IPC_protein 4.151
Toseland 4.062
ProMoST 4.393
Dawson 4.177
Bjellqvist 4.355
Wikipedia 4.177
Rodwell 4.05
Grimsley 4.012
Solomon 4.101
Lehninger 4.062
Nozaki 4.38
DTASelect 4.431
Thurlkill 4.19
EMBOSS 4.177
Sillero 4.304
Patrickios 0.006
IPC_peptide 4.113
IPC2_peptide 4.279
IPC2.peptide.svr19 4.237
Protein with the highest isoelectric point:
>tr|J7K4Y3|J7K4Y3_BPV1 Minor capsid protein L2 OS=Bos grunniens papillomavirus 1 OX=2758380 GN=L2 PE=3 SV=1
MM1 pKa = 7.4 LVFHH5 pKa = 7.24 PPLLILEE12 pKa = 4.69 IAQTGSGSHH21 pKa = 6.35 PRR23 pKa = 11.84 DD24 pKa = 3.19 LKK26 pKa = 9.93 EE27 pKa = 3.62 THH29 pKa = 5.57 QEE31 pKa = 4.03 KK32 pKa = 10.6 KK33 pKa = 10.09 PSQPSLSLLCSAPPPAVPSEE53 pKa = 4.08 QASVGYY59 pKa = 10.0 GIILARR65 pKa = 11.84 TPTIFLQARR74 pKa = 11.84 GAVYY78 pKa = 10.4 SALPPPRR85 pKa = 11.84 CRR87 pKa = 11.84 ARR89 pKa = 11.84 YY90 pKa = 8.6 RR91 pKa = 11.84 WTWHH95 pKa = 4.74 QGRR98 pKa = 11.84 KK99 pKa = 5.95 KK100 pKa = 9.03 TRR102 pKa = 11.84 NNRR105 pKa = 11.84 PTPQRR110 pKa = 11.84 KK111 pKa = 8.37 KK112 pKa = 8.11 QQ113 pKa = 3.25
Molecular weight: 12.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 9.984
IPC_protein 10.994
Toseland 11.125
ProMoST 11.14
Dawson 11.184
Bjellqvist 10.979
Wikipedia 11.477
Rodwell 11.316
Grimsley 11.213
Solomon 11.433
Lehninger 11.374
Nozaki 11.111
DTASelect 10.979
Thurlkill 11.125
EMBOSS 11.564
Sillero 11.125
Patrickios 11.067
IPC_peptide 11.447
IPC2_peptide 10.233
IPC2.peptide.svr19 8.511
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2402
44
606
300.3
33.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.369 ± 0.702
2.456 ± 0.886
5.287 ± 0.465
5.995 ± 0.557
4.33 ± 0.523
7.286 ± 0.703
2.664 ± 0.31
4.08 ± 0.696
5.329 ± 0.783
9.7 ± 0.983
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.291 ± 0.272
3.747 ± 0.619
5.912 ± 1.164
4.163 ± 0.387
5.953 ± 0.858
7.785 ± 0.846
6.744 ± 0.436
5.329 ± 0.413
1.499 ± 0.208
3.081 ± 0.384
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here