Terriglobus roseus
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4053 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G7HQN1|A0A1G7HQN1_9BACT RNA polymerase sigma-70 factor ECF subfamily OS=Terriglobus roseus OX=392734 GN=SAMN05444167_1176 PE=3 SV=1
MM1 pKa = 7.9 SEE3 pKa = 3.98 PRR5 pKa = 11.84 LNNAVTTKK13 pKa = 10.59 DD14 pKa = 3.02 AVAIPEE20 pKa = 4.34 TTRR23 pKa = 11.84 EE24 pKa = 4.05 PIKK27 pKa = 10.33 FAEE30 pKa = 4.5 NLTEE34 pKa = 4.04 VNDD37 pKa = 3.69 NFFFEE42 pKa = 4.82 CGGDD46 pKa = 3.51 GGGDD50 pKa = 3.23 GGDD53 pKa = 3.97 YY54 pKa = 11.28 GDD56 pKa = 5.04 GDD58 pKa = 3.81 WGDD61 pKa = 3.67 GCVPGWDD68 pKa = 4.02 PGCGTWCC75 pKa = 4.09
Molecular weight: 7.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.706
IPC_protein 3.63
Toseland 3.427
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.35
Solomon 3.605
Lehninger 3.567
Nozaki 3.77
DTASelect 3.973
Thurlkill 3.503
EMBOSS 3.605
Sillero 3.757
Patrickios 1.85
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.737
Protein with the highest isoelectric point:
>tr|A0A1G7PWE3|A0A1G7PWE3_9BACT TonB-dependent Receptor Plug Domain OS=Terriglobus roseus OX=392734 GN=SAMN05444167_3650 PE=4 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.02 RR4 pKa = 11.84 TFQPNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.87 THH17 pKa = 5.78 GFLTRR22 pKa = 11.84 MKK24 pKa = 9.05 TKK26 pKa = 10.5 AGQTVLSRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.19 GRR40 pKa = 11.84 HH41 pKa = 5.72 KK42 pKa = 10.29 IAVSAGFRR50 pKa = 11.84 DD51 pKa = 3.66
Molecular weight: 5.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.425
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.369
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.087
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4053
0
4053
1465181
29
2092
361.5
39.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.699 ± 0.039
0.837 ± 0.011
5.179 ± 0.028
5.21 ± 0.047
3.867 ± 0.024
7.956 ± 0.036
2.347 ± 0.02
4.915 ± 0.026
3.864 ± 0.032
9.737 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.387 ± 0.021
3.614 ± 0.036
5.265 ± 0.028
3.927 ± 0.026
5.931 ± 0.039
6.399 ± 0.043
6.449 ± 0.048
7.24 ± 0.029
1.381 ± 0.018
2.795 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here