Piscibacillus halophilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Piscibacillus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3140 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9I7Y6|A0A1H9I7Y6_9BACI 3-isopropylmalate dehydratase small subunit OS=Piscibacillus halophilus OX=571933 GN=leuD PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 10.5LKK4 pKa = 10.68SFWVALLLGLVLVLSACSGGDD25 pKa = 3.35SDD27 pKa = 6.36DD28 pKa = 4.62EE29 pKa = 4.39SAEE32 pKa = 4.34SNGEE36 pKa = 4.12KK37 pKa = 8.98ITIFQTKK44 pKa = 9.59VEE46 pKa = 4.09ISEE49 pKa = 4.1QMEE52 pKa = 4.22EE53 pKa = 3.6LAEE56 pKa = 4.25IYY58 pKa = 10.45EE59 pKa = 4.33EE60 pKa = 4.16EE61 pKa = 4.18TGVEE65 pKa = 4.15VEE67 pKa = 4.2VWGTTGDD74 pKa = 4.73DD75 pKa = 3.85YY76 pKa = 11.41FQQLQTRR83 pKa = 11.84LNSNQGPSILSLQDD97 pKa = 3.06YY98 pKa = 10.44KK99 pKa = 11.02KK100 pKa = 10.99AEE102 pKa = 4.23NIKK105 pKa = 10.72SYY107 pKa = 10.82LHH109 pKa = 6.77DD110 pKa = 4.2LSGEE114 pKa = 4.03EE115 pKa = 4.68FIDD118 pKa = 4.47NIAPNMAVEE127 pKa = 4.09IDD129 pKa = 4.01GEE131 pKa = 4.88VVGIPYY137 pKa = 10.19GVEE140 pKa = 3.97GFGLVYY146 pKa = 10.9NKK148 pKa = 10.72DD149 pKa = 3.62LVSEE153 pKa = 4.45EE154 pKa = 5.26DD155 pKa = 3.32IQDD158 pKa = 3.21YY159 pKa = 11.3DD160 pKa = 3.96SFVNTLEE167 pKa = 4.03RR168 pKa = 11.84LSNEE172 pKa = 3.89GVDD175 pKa = 3.96PLTLSSEE182 pKa = 4.29AYY184 pKa = 10.13FLIGHH189 pKa = 6.36MSNYY193 pKa = 9.28PFSLQEE199 pKa = 3.81DD200 pKa = 3.75HH201 pKa = 7.47FEE203 pKa = 4.69YY204 pKa = 10.08IDD206 pKa = 3.53QLSEE210 pKa = 4.48GEE212 pKa = 4.29VTMTEE217 pKa = 3.9TPEE220 pKa = 3.74FQEE223 pKa = 3.94FGKK226 pKa = 10.34FLEE229 pKa = 5.09AIRR232 pKa = 11.84EE233 pKa = 4.02YY234 pKa = 11.09SPNPMDD240 pKa = 3.41YY241 pKa = 10.29TYY243 pKa = 10.81DD244 pKa = 3.43EE245 pKa = 4.22QMGDD249 pKa = 3.29FATGKK254 pKa = 7.99TAMVHH259 pKa = 4.85QGNWAMGMLADD270 pKa = 3.89YY271 pKa = 10.66DD272 pKa = 4.14YY273 pKa = 11.39DD274 pKa = 4.31FEE276 pKa = 5.21VGMMPFPLAGNDD288 pKa = 3.36KK289 pKa = 10.58LAVGVGANWAVNGTKK304 pKa = 10.42DD305 pKa = 3.32EE306 pKa = 4.54AEE308 pKa = 3.91IEE310 pKa = 4.18AAVDD314 pKa = 3.75FLEE317 pKa = 4.2WLHH320 pKa = 6.0TSEE323 pKa = 3.78TGQRR327 pKa = 11.84FVVEE331 pKa = 4.03EE332 pKa = 4.28FGFVPAMTNIEE343 pKa = 4.28ANDD346 pKa = 4.14LDD348 pKa = 4.16VLSDD352 pKa = 3.39IVLEE356 pKa = 4.09YY357 pKa = 10.97SNSGEE362 pKa = 4.28TIPWAQNYY370 pKa = 8.96YY371 pKa = 8.72PASVVTNDD379 pKa = 4.37FMPAAQDD386 pKa = 3.51FFLDD390 pKa = 3.99EE391 pKa = 5.0NISGEE396 pKa = 4.51DD397 pKa = 4.04FIQLFDD403 pKa = 4.24EE404 pKa = 5.0AWQNAVKK411 pKa = 10.58

Molecular weight: 46.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9LJK3|A0A1H9LJK3_9BACI Uncharacterized protein OS=Piscibacillus halophilus OX=571933 GN=SAMN05216362_14817 PE=4 SV=1
MM1 pKa = 7.42SFLGPHH7 pKa = 6.7LGFGHH12 pKa = 5.77FHH14 pKa = 7.07GFRR17 pKa = 11.84PHH19 pKa = 5.45PRR21 pKa = 11.84FRR23 pKa = 11.84RR24 pKa = 11.84FHH26 pKa = 6.54GFGHH30 pKa = 6.7HH31 pKa = 6.48PRR33 pKa = 11.84FGRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GFGRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84GFGHH48 pKa = 6.13HH49 pKa = 6.87HH50 pKa = 5.88GFKK53 pKa = 10.69PFFF56 pKa = 4.41

Molecular weight: 6.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3140

0

3140

874420

26

3022

278.5

31.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.262 ± 0.041

0.583 ± 0.013

5.709 ± 0.043

8.061 ± 0.064

4.5 ± 0.042

6.736 ± 0.044

2.292 ± 0.021

7.863 ± 0.045

6.332 ± 0.048

9.625 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.765 ± 0.022

4.603 ± 0.033

3.564 ± 0.025

4.176 ± 0.032

3.929 ± 0.033

5.874 ± 0.032

5.24 ± 0.027

7.199 ± 0.039

1.029 ± 0.017

3.658 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski