Ctenophore-associated circular virus 1
Average proteome isoelectric point is 7.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A141MJA6|A0A141MJA6_9VIRU Uncharacterized protein OS=Ctenophore-associated circular virus 1 OX=1778558 PE=4 SV=1
MM1 pKa = 7.14 ATSEE5 pKa = 4.13 TSEE8 pKa = 4.38 TSGGPPPSGTFRR20 pKa = 11.84 AICFTEE26 pKa = 5.28 FGDD29 pKa = 4.07 PPDD32 pKa = 3.6 WDD34 pKa = 4.06 VVQHH38 pKa = 6.44 KK39 pKa = 10.04 LRR41 pKa = 11.84 FMAYY45 pKa = 9.97 GLEE48 pKa = 4.34 TCPTTQRR55 pKa = 11.84 EE56 pKa = 4.58 HH57 pKa = 5.77 YY58 pKa = 10.25 QGFAYY63 pKa = 10.02 AHH65 pKa = 6.7 KK66 pKa = 10.09 PMRR69 pKa = 11.84 WKK71 pKa = 10.12 AWKK74 pKa = 10.01 KK75 pKa = 9.43 LFPRR79 pKa = 11.84 AHH81 pKa = 6.13 FEE83 pKa = 3.85 KK84 pKa = 10.25 MAGSFQDD91 pKa = 3.41 NVVYY95 pKa = 10.48 CSKK98 pKa = 10.51 QSSLIKK104 pKa = 10.51 FGEE107 pKa = 4.18 EE108 pKa = 3.54 PKK110 pKa = 10.6 QGEE113 pKa = 4.39 RR114 pKa = 11.84 KK115 pKa = 10.14 DD116 pKa = 5.07 LIDD119 pKa = 4.46 LKK121 pKa = 11.04 RR122 pKa = 11.84 KK123 pKa = 9.85 LEE125 pKa = 4.27 EE126 pKa = 3.65 YY127 pKa = 10.45 APRR130 pKa = 11.84 RR131 pKa = 11.84 GTTTYY136 pKa = 11.21 DD137 pKa = 3.24 VAEE140 pKa = 4.6 DD141 pKa = 3.36 EE142 pKa = 5.35 AYY144 pKa = 9.76 FGVVARR150 pKa = 11.84 HH151 pKa = 4.75 NTFASGYY158 pKa = 8.79 LRR160 pKa = 11.84 HH161 pKa = 6.26 KK162 pKa = 9.7 RR163 pKa = 11.84 AKK165 pKa = 9.74 ILNGDD170 pKa = 3.15 RR171 pKa = 11.84 TMPKK175 pKa = 8.56 VYY177 pKa = 10.47 VRR179 pKa = 11.84 IGPPGTGKK187 pKa = 8.13 TRR189 pKa = 11.84 WLDD192 pKa = 3.31 RR193 pKa = 11.84 KK194 pKa = 9.97 FGVGGYY200 pKa = 9.89 VIAPDD205 pKa = 3.69 NTGKK209 pKa = 9.72 WFDD212 pKa = 3.85 GCDD215 pKa = 3.23 HH216 pKa = 7.2 DD217 pKa = 6.45 VILFDD222 pKa = 5.59 DD223 pKa = 4.22 VEE225 pKa = 4.81 IDD227 pKa = 3.57 QVPPLSLWKK236 pKa = 9.9 RR237 pKa = 11.84 LCDD240 pKa = 3.6 RR241 pKa = 11.84 YY242 pKa = 10.39 PFEE245 pKa = 4.62 VPVKK249 pKa = 10.65 GGFITWKK256 pKa = 9.0 PRR258 pKa = 11.84 VIVFTSNHH266 pKa = 6.08 AVHH269 pKa = 6.69 EE270 pKa = 4.25 WWKK273 pKa = 10.97 DD274 pKa = 3.1 ITPDD278 pKa = 3.36 NQQAIARR285 pKa = 11.84 RR286 pKa = 11.84 IYY288 pKa = 8.89 KK289 pKa = 8.55 TVYY292 pKa = 10.41 VDD294 pKa = 3.49 GTDD297 pKa = 3.39 GDD299 pKa = 4.0 EE300 pKa = 4.34 TQEE303 pKa = 4.0 EE304 pKa = 4.47 FQSDD308 pKa = 3.97 EE309 pKa = 4.23 DD310 pKa = 4.24 SEE312 pKa = 4.46 GAEE315 pKa = 4.17 EE316 pKa = 4.78 GRR318 pKa = 11.84 AA319 pKa = 3.4
Molecular weight: 36.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.023
IPC2_protein 5.97
IPC_protein 6.059
Toseland 6.415
ProMoST 6.427
Dawson 6.351
Bjellqvist 6.338
Wikipedia 6.364
Rodwell 6.338
Grimsley 6.547
Solomon 6.351
Lehninger 6.351
Nozaki 6.62
DTASelect 6.81
Thurlkill 6.825
EMBOSS 6.795
Sillero 6.722
Patrickios 4.329
IPC_peptide 6.364
IPC2_peptide 6.634
IPC2.peptide.svr19 6.657
Protein with the highest isoelectric point:
>tr|A0A141MJA6|A0A141MJA6_9VIRU Uncharacterized protein OS=Ctenophore-associated circular virus 1 OX=1778558 PE=4 SV=1
MM1 pKa = 7.09 KK2 pKa = 10.09 RR3 pKa = 11.84 KK4 pKa = 9.62 RR5 pKa = 11.84 SSSQTRR11 pKa = 11.84 TVKK14 pKa = 9.9 VRR16 pKa = 11.84 KK17 pKa = 9.17 KK18 pKa = 10.1 AAPKK22 pKa = 10.13 TMTAQRR28 pKa = 11.84 RR29 pKa = 11.84 LIRR32 pKa = 11.84 RR33 pKa = 11.84 MILKK37 pKa = 7.76 TTEE40 pKa = 3.95 TKK42 pKa = 10.87 YY43 pKa = 9.72 KK44 pKa = 10.2 TIALDD49 pKa = 3.55 KK50 pKa = 11.27 EE51 pKa = 4.42 EE52 pKa = 4.59 IYY54 pKa = 10.9 HH55 pKa = 5.76 NKK57 pKa = 10.21 LYY59 pKa = 10.97 NFSKK63 pKa = 10.82 NLLEE67 pKa = 4.25 VFPSQGTGDD76 pKa = 3.84 GARR79 pKa = 11.84 EE80 pKa = 3.74 GDD82 pKa = 3.96 EE83 pKa = 4.05 IVTTGMKK90 pKa = 10.09 VRR92 pKa = 11.84 MILGQKK98 pKa = 9.07 SDD100 pKa = 4.11 RR101 pKa = 11.84 PNVSYY106 pKa = 10.62 KK107 pKa = 10.28 VWVVHH112 pKa = 6.3 YY113 pKa = 10.56 DD114 pKa = 3.44 DD115 pKa = 4.53 STSGNAITYY124 pKa = 10.61 GNFFHH129 pKa = 6.92 NVTGNGMLDD138 pKa = 3.08 AVQNKK143 pKa = 8.65 RR144 pKa = 11.84 FKK146 pKa = 10.43 VLKK149 pKa = 10.55 AFTIKK154 pKa = 10.8 SKK156 pKa = 9.6 GTTIEE161 pKa = 3.9 AGEE164 pKa = 4.08 TSKK167 pKa = 11.26 EE168 pKa = 3.68 FVRR171 pKa = 11.84 PVKK174 pKa = 10.54 FWIPFKK180 pKa = 10.9 KK181 pKa = 10.03 KK182 pKa = 10.52 LKK184 pKa = 9.91 FVSDD188 pKa = 4.3 ASNKK192 pKa = 9.88 ASNILPQMNVIVLPYY207 pKa = 10.05 DD208 pKa = 3.47 AYY210 pKa = 10.35 GTLGTDD216 pKa = 4.01 NIAYY220 pKa = 7.58 MQGSCTLYY228 pKa = 11.06 YY229 pKa = 10.38 KK230 pKa = 10.8 DD231 pKa = 3.73 PP232 pKa = 4.34
Molecular weight: 26.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.104
IPC2_protein 9.443
IPC_protein 9.37
Toseland 10.204
ProMoST 9.794
Dawson 10.35
Bjellqvist 9.97
Wikipedia 10.496
Rodwell 10.965
Grimsley 10.409
Solomon 10.365
Lehninger 10.35
Nozaki 10.175
DTASelect 9.97
Thurlkill 10.218
EMBOSS 10.584
Sillero 10.262
Patrickios 10.643
IPC_peptide 10.379
IPC2_peptide 8.448
IPC2.peptide.svr19 8.487
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
551
232
319
275.5
31.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.989 ± 0.237
1.089 ± 0.404
6.534 ± 1.101
5.808 ± 1.185
5.082 ± 0.474
7.623 ± 0.711
2.178 ± 0.544
4.719 ± 0.544
9.256 ± 1.729
5.082 ± 0.585
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.359 ± 0.669
3.448 ± 1.324
5.082 ± 1.004
3.267 ± 0.153
6.352 ± 0.46
5.082 ± 0.85
7.804 ± 0.767
6.897 ± 0.265
1.996 ± 0.697
4.356 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here