Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces avermitilis

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7672 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q82HM4|Q82HM4_STRAW Putative secreted protein OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_3484 PE=4 SV=1
MM1 pKa = 7.08TGIVVFFFVAAALFVALGLADD22 pKa = 3.5QRR24 pKa = 11.84KK25 pKa = 9.29LYY27 pKa = 9.75WRR29 pKa = 11.84FAARR33 pKa = 11.84RR34 pKa = 11.84YY35 pKa = 9.92RR36 pKa = 11.84DD37 pKa = 3.5PEE39 pKa = 4.07ANEE42 pKa = 4.5PSDD45 pKa = 5.17SAYY48 pKa = 10.24AWQRR52 pKa = 11.84VLIFIAAAVMAFQGFKK68 pKa = 10.9ALDD71 pKa = 3.67FADD74 pKa = 4.38DD75 pKa = 4.14NSWSAGEE82 pKa = 4.32LGQAVEE88 pKa = 4.36QAASALEE95 pKa = 4.19EE96 pKa = 4.27EE97 pKa = 4.38PHH99 pKa = 7.84IDD101 pKa = 5.36DD102 pKa = 4.97EE103 pKa = 4.85YY104 pKa = 11.75SDD106 pKa = 4.16YY107 pKa = 11.62SDD109 pKa = 6.07LIEE112 pKa = 5.47QEE114 pKa = 4.25VEE116 pKa = 4.13DD117 pKa = 4.77AGEE120 pKa = 4.22DD121 pKa = 3.53MGPGYY126 pKa = 10.76AVNVEE131 pKa = 4.24PADD134 pKa = 3.63SRR136 pKa = 11.84DD137 pKa = 3.7DD138 pKa = 3.87YY139 pKa = 11.44EE140 pKa = 4.24ITAEE144 pKa = 4.21GTDD147 pKa = 3.09AAYY150 pKa = 10.82CMSVSQKK157 pKa = 10.58EE158 pKa = 4.07SDD160 pKa = 3.28EE161 pKa = 4.66GGFTVPGVGDD171 pKa = 3.43QQGTYY176 pKa = 9.35VPEE179 pKa = 4.32YY180 pKa = 10.44DD181 pKa = 4.78LSATTAEE188 pKa = 4.98GACC191 pKa = 3.96

Molecular weight: 20.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q82FE0|RNPA_STRAW Ribonuclease P protein component OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=rnpA PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.5GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7672

0

7672

2610487

29

7746

340.3

36.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.443 ± 0.042

0.808 ± 0.009

5.938 ± 0.024

5.607 ± 0.028

2.728 ± 0.016

9.489 ± 0.03

2.349 ± 0.018

3.138 ± 0.024

2.302 ± 0.026

10.218 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.721 ± 0.013

1.81 ± 0.015

6.096 ± 0.031

2.852 ± 0.016

7.995 ± 0.034

5.262 ± 0.023

6.237 ± 0.024

8.346 ± 0.026

1.542 ± 0.012

2.118 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski