Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5201 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B4UMX9|B4UMX9_CANGA Uncharacterized protein OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=CAGL0B03899g PE=4 SV=1
MM1 pKa = 7.21 NEE3 pKa = 3.89 KK4 pKa = 9.96 IFRR7 pKa = 11.84 YY8 pKa = 8.33 LTLLFLFEE16 pKa = 4.89 LASSLPVEE24 pKa = 4.87 DD25 pKa = 4.25 KK26 pKa = 11.64 LNIKK30 pKa = 10.07 KK31 pKa = 10.12 VFEE34 pKa = 3.96 KK35 pKa = 10.44 RR36 pKa = 11.84 AVDD39 pKa = 4.07 FTPFAHH45 pKa = 6.29 YY46 pKa = 7.94 PRR48 pKa = 11.84 PEE50 pKa = 4.59 GCSSPPNSVSVGLHH64 pKa = 4.79 MNLYY68 pKa = 9.29 NYY70 pKa = 9.15 PYY72 pKa = 10.41 LYY74 pKa = 10.59 VKK76 pKa = 10.45 PSTKK80 pKa = 10.62 GMFTNDD86 pKa = 3.39 TNSDD90 pKa = 3.4 SDD92 pKa = 4.18 GEE94 pKa = 4.35 TDD96 pKa = 3.48 GDD98 pKa = 3.97 SAGGIEE104 pKa = 5.14 GRR106 pKa = 11.84 AGQCWNPEE114 pKa = 4.0 YY115 pKa = 10.27 QDD117 pKa = 3.51 PNFPRR122 pKa = 11.84 FGYY125 pKa = 9.27 KK126 pKa = 9.71 QYY128 pKa = 11.68 GEE130 pKa = 5.0 FGASDD135 pKa = 3.89 NVNGDD140 pKa = 3.14 ISWDD144 pKa = 3.49 HH145 pKa = 6.78 NEE147 pKa = 3.87 FTEE150 pKa = 4.46 GCKK153 pKa = 9.77 PVLAALPPGYY163 pKa = 10.12 NYY165 pKa = 9.85 PDD167 pKa = 4.87 EE168 pKa = 4.31 ITFSNFTMVLSGYY181 pKa = 8.69 FKK183 pKa = 10.65 PKK185 pKa = 9.3 TSGVYY190 pKa = 10.11 KK191 pKa = 10.73 FEE193 pKa = 4.77 LKK195 pKa = 10.66 ADD197 pKa = 3.83 DD198 pKa = 5.31 FILFNFGAKK207 pKa = 9.43 NAFEE211 pKa = 4.31 CCNRR215 pKa = 11.84 EE216 pKa = 3.63 EE217 pKa = 4.92 SIDD220 pKa = 3.65 NFGPYY225 pKa = 8.59 VAYY228 pKa = 10.53 AMWPNQADD236 pKa = 3.62 QEE238 pKa = 4.58 LEE240 pKa = 4.13 VYY242 pKa = 10.5 LFEE245 pKa = 6.18 DD246 pKa = 3.4 SYY248 pKa = 11.61 YY249 pKa = 10.22 PLRR252 pKa = 11.84 LFYY255 pKa = 11.07 NNRR258 pKa = 11.84 DD259 pKa = 3.39 YY260 pKa = 11.25 HH261 pKa = 7.21 SKK263 pKa = 10.61 FKK265 pKa = 10.68 IGFYY269 pKa = 10.53 PPGQTTITFDD279 pKa = 3.23 FDD281 pKa = 3.85 GYY283 pKa = 11.54 LFMLDD288 pKa = 3.63 DD289 pKa = 4.39 TGNEE293 pKa = 3.98 CRR295 pKa = 11.84 DD296 pKa = 3.67 SIRR299 pKa = 11.84 YY300 pKa = 7.46 RR301 pKa = 11.84 TVCDD305 pKa = 4.11 DD306 pKa = 3.93 KK307 pKa = 11.87 VVDD310 pKa = 3.77 DD311 pKa = 3.93 TTYY314 pKa = 8.5 STRR317 pKa = 11.84 YY318 pKa = 6.78 STSMVPGGGSDD329 pKa = 3.72 VITIYY334 pKa = 10.14 YY335 pKa = 9.9 IRR337 pKa = 11.84 MKK339 pKa = 10.74 CEE341 pKa = 3.83 EE342 pKa = 4.58 EE343 pKa = 4.5 DD344 pKa = 5.7 CLGQWDD350 pKa = 5.16 PINNVCYY357 pKa = 10.83 SPDD360 pKa = 3.42 DD361 pKa = 4.01 CADD364 pKa = 4.21 DD365 pKa = 3.77 GGYY368 pKa = 10.18 WNGQMCDD375 pKa = 3.46 QSCKK379 pKa = 10.61 LEE381 pKa = 4.18 GGIVNPDD388 pKa = 3.07 TGDD391 pKa = 3.55 CDD393 pKa = 3.79 KK394 pKa = 11.66 SCIEE398 pKa = 4.18 SGGFLDD404 pKa = 5.56 EE405 pKa = 5.62 NGNCDD410 pKa = 3.45 TTCRR414 pKa = 11.84 DD415 pKa = 3.55 DD416 pKa = 5.09 GGMLVEE422 pKa = 4.61 GQCDD426 pKa = 4.04 YY427 pKa = 11.14 QCKK430 pKa = 8.82 EE431 pKa = 3.7 AGGILVGDD439 pKa = 4.3 HH440 pKa = 7.42 CDD442 pKa = 3.6 TTCVDD447 pKa = 3.85 SGGKK451 pKa = 10.02 LNEE454 pKa = 5.64 DD455 pKa = 3.63 GTCDD459 pKa = 4.13 HH460 pKa = 7.05 SCRR463 pKa = 11.84 DD464 pKa = 3.12 QGGQLDD470 pKa = 4.03 EE471 pKa = 5.47 SGEE474 pKa = 4.34 CDD476 pKa = 3.59 TSCKK480 pKa = 10.4 DD481 pKa = 3.23 SGGMLIEE488 pKa = 4.78 GEE490 pKa = 4.73 CDD492 pKa = 3.32 TSCKK496 pKa = 10.63 DD497 pKa = 3.25 EE498 pKa = 4.82 GGQLDD503 pKa = 4.55 EE504 pKa = 5.04 NNEE507 pKa = 4.5 CINHH511 pKa = 6.33 CKK513 pKa = 10.26 DD514 pKa = 2.84 QGGIIDD520 pKa = 4.27 EE521 pKa = 4.89 NGNCDD526 pKa = 3.84 TSCKK530 pKa = 10.42 DD531 pKa = 3.15 SGGKK535 pKa = 9.68 LDD537 pKa = 4.85 EE538 pKa = 5.33 NGACDD543 pKa = 3.66 TSCRR547 pKa = 11.84 DD548 pKa = 3.01 EE549 pKa = 4.97 GGIIDD554 pKa = 4.91 EE555 pKa = 4.95 NGNCNTTCRR564 pKa = 11.84 DD565 pKa = 3.38 DD566 pKa = 4.5 GGILVGDD573 pKa = 4.29 HH574 pKa = 7.4 CDD576 pKa = 3.43 TSCRR580 pKa = 11.84 DD581 pKa = 3.19 AGGILEE587 pKa = 5.57 DD588 pKa = 5.02 GICNSTCRR596 pKa = 11.84 DD597 pKa = 3.26 SGGKK601 pKa = 10.36 LNDD604 pKa = 5.33 DD605 pKa = 3.68 GTCDD609 pKa = 3.67 YY610 pKa = 11.26 SCRR613 pKa = 11.84 DD614 pKa = 2.96 QGGQLDD620 pKa = 4.13 EE621 pKa = 5.96 NGDD624 pKa = 3.82 CDD626 pKa = 4.25 YY627 pKa = 11.37 SCKK630 pKa = 10.69 NGGGKK635 pKa = 9.67 LDD637 pKa = 3.75 EE638 pKa = 6.11 DD639 pKa = 5.1 GNCDD643 pKa = 3.5 MSCKK647 pKa = 10.31 EE648 pKa = 4.33 NGGQLDD654 pKa = 3.85 EE655 pKa = 7.07 DD656 pKa = 4.76 GDD658 pKa = 4.44 CDD660 pKa = 5.28 LSCKK664 pKa = 10.21 EE665 pKa = 5.12 DD666 pKa = 3.39 GGKK669 pKa = 10.22 LDD671 pKa = 4.6 GNGDD675 pKa = 3.67 CDD677 pKa = 4.07 YY678 pKa = 11.24 SCKK681 pKa = 10.51 EE682 pKa = 3.66 EE683 pKa = 4.3 GGIIDD688 pKa = 4.6 EE689 pKa = 4.92 SGNCNTTCKK698 pKa = 10.55 DD699 pKa = 3.06 SGGQIIDD706 pKa = 3.76 GEE708 pKa = 4.54 CNYY711 pKa = 10.91 GCIEE715 pKa = 4.18 VGGVVIDD722 pKa = 4.55 GEE724 pKa = 4.7 CDD726 pKa = 3.43 TSCVDD731 pKa = 4.21 SGGILDD737 pKa = 5.01 GDD739 pKa = 4.47 GNCDD743 pKa = 3.22 RR744 pKa = 11.84 SCIEE748 pKa = 3.85 SGGVLEE754 pKa = 6.27 DD755 pKa = 4.18 GACNTTCRR763 pKa = 11.84 DD764 pKa = 3.03 SGGKK768 pKa = 10.32 LNVDD772 pKa = 4.18 GTCDD776 pKa = 3.38 TSCRR780 pKa = 11.84 DD781 pKa = 3.54 DD782 pKa = 4.35 GGMLVEE788 pKa = 4.61 GQCDD792 pKa = 4.04 YY793 pKa = 11.14 QCKK796 pKa = 8.86 EE797 pKa = 3.77 AGGILEE803 pKa = 5.3 DD804 pKa = 4.58 GEE806 pKa = 5.47 CVFPPTSSSTSEE818 pKa = 4.07 TSSTTDD824 pKa = 3.08 QSSSQSDD831 pKa = 3.59 DD832 pKa = 3.55 SSVEE836 pKa = 4.18 SSSTPMDD843 pKa = 3.31 SSTIEE848 pKa = 4.05 SSSSEE853 pKa = 4.05 IITSEE858 pKa = 4.19 SEE860 pKa = 4.28 SEE862 pKa = 4.38 SVPIASSSSEE872 pKa = 3.83 VMTSEE877 pKa = 4.71 SEE879 pKa = 4.33 SEE881 pKa = 4.4 SIPILTSSSEE891 pKa = 3.85 IPIASSSSEE900 pKa = 3.74 IIIPEE905 pKa = 4.16 SEE907 pKa = 4.3 SEE909 pKa = 4.46 SVPVASSSSEE919 pKa = 3.71 IPVASSSSEE928 pKa = 3.72 IIIPEE933 pKa = 4.16 SEE935 pKa = 4.34 SEE937 pKa = 4.44 SVPIASSSSEE947 pKa = 3.77 IPVASSSSEE956 pKa = 3.72 IIIPEE961 pKa = 4.18 SEE963 pKa = 4.29 SEE965 pKa = 4.35 SIPIEE970 pKa = 4.36 STSSEE975 pKa = 4.1 AVSSRR980 pKa = 11.84 SEE982 pKa = 4.0 SDD984 pKa = 2.79 SSYY987 pKa = 9.35 PTSTPTTTTSTTWPNPIQPTTSNSSPIVSSSTEE1020 pKa = 3.83 TPGIAEE1026 pKa = 4.02 SSSRR1030 pKa = 11.84 ISDD1033 pKa = 3.81 LEE1035 pKa = 3.9 SSRR1038 pKa = 11.84 RR1039 pKa = 11.84 TSILGDD1045 pKa = 3.14 DD1046 pKa = 3.92 RR1047 pKa = 11.84 TRR1049 pKa = 11.84 TRR1051 pKa = 11.84 TTITSTITEE1060 pKa = 4.73 DD1061 pKa = 3.52 GQIITITTTVTGFVPEE1077 pKa = 4.24 PTGEE1081 pKa = 4.24 TEE1083 pKa = 4.82 GNGSDD1088 pKa = 5.49 DD1089 pKa = 4.14 EE1090 pKa = 4.46 NLEE1093 pKa = 4.1 NPIEE1097 pKa = 4.34 NNTDD1101 pKa = 2.97 EE1102 pKa = 5.07 NEE1104 pKa = 4.2 FVDD1107 pKa = 5.76 PEE1109 pKa = 4.24 FTDD1112 pKa = 6.26 DD1113 pKa = 5.61 IPDD1116 pKa = 4.17 DD1117 pKa = 3.94 PAGPEE1122 pKa = 4.07 DD1123 pKa = 4.06 NGNGPVNPEE1132 pKa = 3.76 QGNDD1136 pKa = 3.34 NDD1138 pKa = 4.42 PVNLEE1143 pKa = 3.96 DD1144 pKa = 4.28 SGNNGAVNPEE1154 pKa = 3.77 QAPVNDD1160 pKa = 4.36 EE1161 pKa = 4.64 DD1162 pKa = 4.9 NGGNDD1167 pKa = 3.95 PVNPEE1172 pKa = 3.53 QDD1174 pKa = 3.86 NNNGPANPGQDD1185 pKa = 3.3 NTNGSSNSDD1194 pKa = 3.35 QIDD1197 pKa = 3.89 NNPPTPEE1204 pKa = 3.88 EE1205 pKa = 3.83 NAGDD1209 pKa = 4.05 GNSNGNDD1216 pKa = 3.42 PADD1219 pKa = 3.84 PSRR1222 pKa = 11.84 RR1223 pKa = 11.84 TSILGDD1229 pKa = 3.2 EE1230 pKa = 4.64 RR1231 pKa = 11.84 TRR1233 pKa = 11.84 ASPGGTSQPTGANGNVNGNSPGANVNGGGTGNSNNNNNNNNNNNNNNNNGNAPNGGDD1290 pKa = 4.24 GSNGDD1295 pKa = 3.97 ANQGPSRR1302 pKa = 11.84 RR1303 pKa = 11.84 TSLLGDD1309 pKa = 3.27 EE1310 pKa = 4.53 RR1311 pKa = 11.84 TRR1313 pKa = 11.84 AGQAAPTGGSSPNNGVAGDD1332 pKa = 3.66 TSNNGGAIVSQQVDD1346 pKa = 3.47 DD1347 pKa = 5.02 GGAGSPRR1354 pKa = 11.84 RR1355 pKa = 11.84 TSILGDD1361 pKa = 3.18 VRR1363 pKa = 11.84 SRR1365 pKa = 11.84 TTLNGEE1371 pKa = 4.05 VLNFEE1376 pKa = 4.37 GSGVRR1381 pKa = 11.84 RR1382 pKa = 11.84 AVAGNLALPLMMVGLVFNLL1401 pKa = 3.56
Molecular weight: 148.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.77
IPC_protein 3.808
Toseland 3.579
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.884
DTASelect 4.126
Thurlkill 3.617
EMBOSS 3.719
Sillero 3.923
Patrickios 0.693
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|F2Z629|F2Z629_CANGA Ribosomal protein L37 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=CAGL0B01203g PE=3 SV=1
MM1 pKa = 7.82 PAQKK5 pKa = 10.31 SFKK8 pKa = 10.07 IKK10 pKa = 10.38 QKK12 pKa = 9.96 LAKK15 pKa = 9.88 AKK17 pKa = 9.79 KK18 pKa = 8.92 QNRR21 pKa = 11.84 TLPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TNNTIRR36 pKa = 11.84 YY37 pKa = 5.73 NAKK40 pKa = 8.47 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.01 WRR45 pKa = 11.84 RR46 pKa = 11.84 TKK48 pKa = 10.52 MNII51 pKa = 3.1
Molecular weight: 6.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.366
IPC2_protein 11.023
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.384
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.106
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 8.974
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5200
1
5201
2635782
34
4880
506.8
57.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.532 ± 0.031
1.161 ± 0.013
6.163 ± 0.028
6.76 ± 0.036
4.183 ± 0.025
5.247 ± 0.045
2.11 ± 0.013
6.523 ± 0.029
7.301 ± 0.035
9.315 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.211 ± 0.013
6.035 ± 0.028
4.25 ± 0.028
3.928 ± 0.027
4.435 ± 0.022
8.682 ± 0.058
5.852 ± 0.041
5.833 ± 0.024
0.991 ± 0.009
3.488 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here