Rhodococcus phage RRH1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9FGW5|G9FGW5_9CAUD HNH endonuclease OS=Rhodococcus phage RRH1 OX=1109717 PE=4 SV=1
MM1 pKa = 7.27TRR3 pKa = 11.84TSAGSSSHH11 pKa = 6.58PGVTVRR17 pKa = 11.84VMSAYY22 pKa = 10.43SPNDD26 pKa = 3.1EE27 pKa = 4.52RR28 pKa = 11.84SLHH31 pKa = 5.8AAAILASEE39 pKa = 4.44HH40 pKa = 6.6LSGIQLLAYY49 pKa = 10.07AVGKK53 pKa = 10.14LGNDD57 pKa = 3.7DD58 pKa = 4.17RR59 pKa = 11.84PQLVDD64 pKa = 3.09AARR67 pKa = 11.84YY68 pKa = 9.75LEE70 pKa = 4.26VVGDD74 pKa = 4.21RR75 pKa = 11.84LASAVSLLFTEE86 pKa = 5.31LGEE89 pKa = 4.47ATDD92 pKa = 4.44PDD94 pKa = 4.37PEE96 pKa = 4.43LFAVDD101 pKa = 3.29TDD103 pKa = 3.94RR104 pKa = 11.84PVPSVRR110 pKa = 11.84VIPAGAPTGDD120 pKa = 4.43PDD122 pKa = 4.81PSIDD126 pKa = 4.09QAPDD130 pKa = 3.45PAPADD135 pKa = 3.64PPATRR140 pKa = 11.84SCADD144 pKa = 3.32SQDD147 pKa = 4.08PGSVPSDD154 pKa = 3.37LL155 pKa = 4.59

Molecular weight: 16.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9FGW3|G9FGW3_9CAUD Uncharacterized protein OS=Rhodococcus phage RRH1 OX=1109717 PE=4 SV=1
MM1 pKa = 6.51TTLTASDD8 pKa = 3.62RR9 pKa = 11.84DD10 pKa = 3.7YY11 pKa = 11.03LTRR14 pKa = 11.84NTHH17 pKa = 5.03AHH19 pKa = 6.74LDD21 pKa = 3.54ALGVSRR27 pKa = 11.84RR28 pKa = 11.84AKK30 pKa = 8.29TVRR33 pKa = 11.84AVAVEE38 pKa = 3.97LTVTAATYY46 pKa = 10.27QDD48 pKa = 3.72HH49 pKa = 7.65ADD51 pKa = 3.69GNGNYY56 pKa = 9.79YY57 pKa = 10.29VFPSVDD63 pKa = 3.05YY64 pKa = 10.44LANHH68 pKa = 7.33RR69 pKa = 11.84YY70 pKa = 9.85VMATVRR76 pKa = 11.84GVHH79 pKa = 6.28KK80 pKa = 10.61ALAVLRR86 pKa = 11.84DD87 pKa = 3.53VGFLPKK93 pKa = 10.3VIHH96 pKa = 6.32TGRR99 pKa = 11.84AAWRR103 pKa = 11.84AVTGTVVDD111 pKa = 4.95AWNKK115 pKa = 7.16WKK117 pKa = 10.89SRR119 pKa = 11.84GRR121 pKa = 11.84DD122 pKa = 3.19VDD124 pKa = 3.76PAAPAPRR131 pKa = 11.84FRR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84AQRR138 pKa = 11.84SGHH141 pKa = 6.46APTTVQVATTYY152 pKa = 10.9SSEE155 pKa = 4.25EE156 pKa = 4.07KK157 pKa = 10.76KK158 pKa = 9.43NTPVSAAQRR167 pKa = 11.84PSWATSPTSAPTPVPPPFVPSDD189 pKa = 3.83SEE191 pKa = 4.29GSSDD195 pKa = 4.47DD196 pKa = 3.39VRR198 pKa = 11.84AAAMAAIRR206 pKa = 11.84RR207 pKa = 11.84TLGTKK212 pKa = 9.37KK213 pKa = 10.25RR214 pKa = 11.84RR215 pKa = 3.56

Molecular weight: 23.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

4297

39

661

214.8

22.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.685 ± 1.507

0.582 ± 0.19

7.238 ± 0.511

3.63 ± 0.337

2.746 ± 0.181

8.122 ± 0.4

1.838 ± 0.232

4.747 ± 0.466

1.815 ± 0.269

6.726 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.652 ± 0.182

3.025 ± 0.335

6.912 ± 0.607

2.839 ± 0.286

7.47 ± 0.733

6.074 ± 0.302

7.377 ± 0.554

8.471 ± 0.455

2.048 ± 0.321

2.001 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski