Human immunodeficiency virus type 2 subtype B (isolate UC1) (HIV-2)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 2; HIV-2 subtype B

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q76638|ENV_HV2UC Envelope glycoprotein gp160 OS=Human immunodeficiency virus type 2 subtype B (isolate UC1) OX=388822 GN=env PE=3 SV=1
MM1 pKa = 7.65AEE3 pKa = 4.61AAPEE7 pKa = 4.04TPPEE11 pKa = 4.14NEE13 pKa = 4.1SPQRR17 pKa = 11.84EE18 pKa = 4.11PWEE21 pKa = 3.98EE22 pKa = 3.7WVEE25 pKa = 4.07DD26 pKa = 3.5VMEE29 pKa = 4.85EE30 pKa = 3.9IKK32 pKa = 10.8QEE34 pKa = 4.05ALRR37 pKa = 11.84HH38 pKa = 5.31FDD40 pKa = 3.54PRR42 pKa = 11.84LLTALGNFIYY52 pKa = 10.37SRR54 pKa = 11.84HH55 pKa = 5.95GDD57 pKa = 3.5TLAGAGEE64 pKa = 4.73LIKK67 pKa = 10.37ILQRR71 pKa = 11.84ALFLHH76 pKa = 6.52FRR78 pKa = 11.84AGCQHH83 pKa = 6.08SRR85 pKa = 11.84IGQPGGGNPLSAIPPSS101 pKa = 3.47

Molecular weight: 11.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q76636|VPX_HV2UC Protein Vpx OS=Human immunodeficiency virus type 2 subtype B (isolate UC1) OX=388822 GN=vpx PE=3 SV=1
MM1 pKa = 7.57EE2 pKa = 5.37GEE4 pKa = 4.47KK5 pKa = 10.49NWIVVPTWRR14 pKa = 11.84IPGRR18 pKa = 11.84LEE20 pKa = 3.54KK21 pKa = 9.76WHH23 pKa = 6.86SLVKK27 pKa = 10.2YY28 pKa = 9.76LKK30 pKa = 10.37HH31 pKa = 5.59RR32 pKa = 11.84TKK34 pKa = 10.73EE35 pKa = 4.04LQQVSYY41 pKa = 10.92VPHH44 pKa = 6.83HH45 pKa = 6.05KK46 pKa = 10.78VGWAWWTCSRR56 pKa = 11.84VIFPLKK62 pKa = 10.1EE63 pKa = 3.45EE64 pKa = 4.93AYY66 pKa = 10.96LEE68 pKa = 4.25VQGYY72 pKa = 8.36WNLTPEE78 pKa = 4.22RR79 pKa = 11.84GFLSSYY85 pKa = 10.58AVRR88 pKa = 11.84LTWYY92 pKa = 9.84KK93 pKa = 10.43RR94 pKa = 11.84SFYY97 pKa = 10.77TDD99 pKa = 3.15VTPDD103 pKa = 3.08VADD106 pKa = 3.62QLLHH110 pKa = 6.63GSYY113 pKa = 10.55FSCFTANEE121 pKa = 3.53VRR123 pKa = 11.84RR124 pKa = 11.84AIRR127 pKa = 11.84GEE129 pKa = 4.35KK130 pKa = 9.22ILSYY134 pKa = 10.81CNYY137 pKa = 9.14PSAHH141 pKa = 6.74EE142 pKa = 4.22GQVPSLQFLALRR154 pKa = 11.84VIQEE158 pKa = 4.49GKK160 pKa = 10.22DD161 pKa = 3.59GSQGEE166 pKa = 4.53SATRR170 pKa = 11.84KK171 pKa = 7.55QRR173 pKa = 11.84RR174 pKa = 11.84RR175 pKa = 11.84NNRR178 pKa = 11.84RR179 pKa = 11.84SIRR182 pKa = 11.84LARR185 pKa = 11.84KK186 pKa = 9.09NNNRR190 pKa = 11.84AQQGSSQPLAPRR202 pKa = 11.84THH204 pKa = 6.54FPGLAEE210 pKa = 4.07VLGILAA216 pKa = 4.81

Molecular weight: 25.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3877

97

1471

387.7

43.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.397 ± 0.477

2.399 ± 0.35

3.998 ± 0.335

7.248 ± 0.597

2.502 ± 0.239

7.274 ± 0.53

2.167 ± 0.212

5.236 ± 0.668

6.345 ± 0.678

8.512 ± 0.357

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.309

4.436 ± 0.571

6.216 ± 0.721

5.726 ± 0.316

6.242 ± 0.739

5.52 ± 1.211

6.319 ± 0.877

5.623 ± 0.703

2.45 ± 0.266

3.173 ± 0.381
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski