Torque teno mini virus 7
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9JG59|Q9JG59_9VIRU Uncharacterized protein OS=Torque teno mini virus 7 OX=687375 PE=4 SV=1
MM1 pKa = 7.71 SRR3 pKa = 11.84 QCPPKK8 pKa = 10.63 YY9 pKa = 10.22 NNKK12 pKa = 9.0 QLQLQHH18 pKa = 6.35 VNTLVSIHH26 pKa = 7.35 DD27 pKa = 3.99 LTCGCEE33 pKa = 4.2 SPLEE37 pKa = 4.02 CTIITIYY44 pKa = 10.61 NQEE47 pKa = 4.0 KK48 pKa = 10.12 DD49 pKa = 3.54 LKK51 pKa = 9.84 FTEE54 pKa = 4.31 EE55 pKa = 4.13 EE56 pKa = 4.11 KK57 pKa = 11.01 KK58 pKa = 10.66 KK59 pKa = 9.22 LQKK62 pKa = 9.98 WLTSTEE68 pKa = 3.97 KK69 pKa = 10.35 DD70 pKa = 3.29 TTPEE74 pKa = 3.79 EE75 pKa = 4.15 EE76 pKa = 4.48 GFGDD80 pKa = 4.14 GDD82 pKa = 3.96 LEE84 pKa = 4.46 RR85 pKa = 11.84 LFDD88 pKa = 4.49 EE89 pKa = 5.95 DD90 pKa = 5.26 FGDD93 pKa = 5.0 DD94 pKa = 3.75 ADD96 pKa = 3.96 TATRR100 pKa = 3.75
Molecular weight: 11.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.548
IPC2_protein 4.52
IPC_protein 4.431
Toseland 4.266
ProMoST 4.533
Dawson 4.38
Bjellqvist 4.52
Wikipedia 4.253
Rodwell 4.266
Grimsley 4.177
Solomon 4.368
Lehninger 4.329
Nozaki 4.482
DTASelect 4.647
Thurlkill 4.279
EMBOSS 4.266
Sillero 4.533
Patrickios 3.846
IPC_peptide 4.38
IPC2_peptide 4.533
IPC2.peptide.svr19 4.48
Protein with the highest isoelectric point:
>tr|Q9JG59|Q9JG59_9VIRU Uncharacterized protein OS=Torque teno mini virus 7 OX=687375 PE=4 SV=1
MM1 pKa = 7.72 AYY3 pKa = 10.08 FYY5 pKa = 10.84 RR6 pKa = 11.84 KK7 pKa = 9.08 RR8 pKa = 11.84 YY9 pKa = 7.24 YY10 pKa = 9.25 PRR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 FWRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 PRR22 pKa = 11.84 APFRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 FWRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 YY35 pKa = 9.07 RR36 pKa = 11.84 YY37 pKa = 9.15 KK38 pKa = 10.59 VKK40 pKa = 10.64 RR41 pKa = 11.84 KK42 pKa = 10.13 LSTIKK47 pKa = 10.45 LKK49 pKa = 10.1 QWQPIYY55 pKa = 10.51 LRR57 pKa = 11.84 HH58 pKa = 5.83 LKK60 pKa = 10.22 IKK62 pKa = 10.68 GILPLFITTDD72 pKa = 3.15 KK73 pKa = 10.83 RR74 pKa = 11.84 ISNDD78 pKa = 2.62 YY79 pKa = 10.18 TMYY82 pKa = 10.38 TNSLAQHH89 pKa = 5.68 YY90 pKa = 9.7 VPGGGGFTIYY100 pKa = 10.76 RR101 pKa = 11.84 FTLKK105 pKa = 10.61 ALYY108 pKa = 10.19 EE109 pKa = 4.46 LFTKK113 pKa = 10.11 IQNKK117 pKa = 5.56 WTQSNEE123 pKa = 3.32 DD124 pKa = 3.4 WPFIRR129 pKa = 11.84 YY130 pKa = 7.25 TGCEE134 pKa = 3.68 FKK136 pKa = 10.49 FYY138 pKa = 10.62 KK139 pKa = 10.0 SEE141 pKa = 3.92 KK142 pKa = 9.39 ADD144 pKa = 3.72 YY145 pKa = 9.82 IINYY149 pKa = 7.78 HH150 pKa = 6.47 RR151 pKa = 11.84 CPPMLATIDD160 pKa = 4.21 TFHH163 pKa = 6.48 STQPHH168 pKa = 5.59 MMTLNKK174 pKa = 8.78 KK175 pKa = 9.91 HH176 pKa = 6.57 KK177 pKa = 10.17 IIRR180 pKa = 11.84 CKK182 pKa = 10.32 QNNPYY187 pKa = 9.35 KK188 pKa = 10.43 RR189 pKa = 11.84 PYY191 pKa = 9.94 KK192 pKa = 9.78 RR193 pKa = 11.84 VKK195 pKa = 9.97 IKK197 pKa = 10.67 PPALLQNNWYY207 pKa = 8.6 FQKK210 pKa = 10.98 DD211 pKa = 3.65 FAAEE215 pKa = 3.86 PLLLLMTSSMSLDD228 pKa = 3.0 RR229 pKa = 11.84 YY230 pKa = 9.61 YY231 pKa = 11.35 LNSKK235 pKa = 10.13 SISTTMGFISLNDD248 pKa = 3.89 NIFDD252 pKa = 3.9 YY253 pKa = 11.31 YY254 pKa = 11.05 DD255 pKa = 3.54 YY256 pKa = 11.19 KK257 pKa = 11.15 LPPTTGYY264 pKa = 9.8 IPKK267 pKa = 8.91 PKK269 pKa = 10.15 KK270 pKa = 10.68 YY271 pKa = 8.68 MWSLYY276 pKa = 10.17 QPLADD281 pKa = 3.4 IHH283 pKa = 5.61 KK284 pKa = 7.96 TQIKK288 pKa = 9.37 EE289 pKa = 3.92 LIYY292 pKa = 10.41 LGKK295 pKa = 10.16 SKK297 pKa = 10.58 EE298 pKa = 3.98 MDD300 pKa = 2.8 KK301 pKa = 11.4 GKK303 pKa = 9.42 PLKK306 pKa = 10.69 NFLHH310 pKa = 6.98 GDD312 pKa = 3.59 PDD314 pKa = 4.12 EE315 pKa = 4.22 TWTNYY320 pKa = 9.44 FSSTQNWGNIFDD332 pKa = 4.81 PYY334 pKa = 11.21 YY335 pKa = 9.51 LTQEE339 pKa = 4.21 HH340 pKa = 5.58 VVLYY344 pKa = 10.09 SEE346 pKa = 5.23 KK347 pKa = 10.88 SPNEE351 pKa = 3.81 LKK353 pKa = 10.01 TEE355 pKa = 4.0 YY356 pKa = 10.61 QKK358 pKa = 11.41 DD359 pKa = 3.46 KK360 pKa = 11.26 QKK362 pKa = 10.72 WKK364 pKa = 9.02 TFSNEE369 pKa = 2.96 IGEE372 pKa = 4.58 YY373 pKa = 9.56 IQEE376 pKa = 4.33 TTHH379 pKa = 6.41 TLLVNCRR386 pKa = 11.84 YY387 pKa = 10.26 NPYY390 pKa = 10.71 ADD392 pKa = 3.34 TGGNTVFIVPITQPEE407 pKa = 4.13 QTPLHH412 pKa = 6.47 IPQDD416 pKa = 3.88 DD417 pKa = 4.06 KK418 pKa = 11.9 YY419 pKa = 9.81 KK420 pKa = 10.2 TEE422 pKa = 5.28 PYY424 pKa = 9.88 PLWLGTWGFLDD435 pKa = 3.96 YY436 pKa = 10.55 EE437 pKa = 4.47 RR438 pKa = 11.84 KK439 pKa = 10.23 RR440 pKa = 11.84 IGQTIDD446 pKa = 2.61 TDD448 pKa = 3.79 YY449 pKa = 11.38 QIVIKK454 pKa = 10.27 SPHH457 pKa = 5.82 ITPSQYY463 pKa = 10.44 FYY465 pKa = 11.09 IPIDD469 pKa = 3.46 EE470 pKa = 4.51 TFTTGRR476 pKa = 11.84 SPYY479 pKa = 9.73 RR480 pKa = 11.84 PPDD483 pKa = 3.8 TQPTVYY489 pKa = 10.39 DD490 pKa = 3.53 QLHH493 pKa = 5.54 WHH495 pKa = 6.83 PKK497 pKa = 8.07 ITMQMDD503 pKa = 4.91 TINSIAACGPGTIKK517 pKa = 10.88 LPPQVSAEE525 pKa = 4.03 AHH527 pKa = 5.12 CTYY530 pKa = 10.39 TFHH533 pKa = 7.6 FKK535 pKa = 10.65 LGSCAPPMQNIDD547 pKa = 3.57 NPEE550 pKa = 3.95 KK551 pKa = 10.89 QEE553 pKa = 3.96 TFANNIFQTNSLQNPEE569 pKa = 4.62 YY570 pKa = 9.59 PLQYY574 pKa = 10.72 YY575 pKa = 9.98 LYY577 pKa = 10.54 NFDD580 pKa = 4.37 EE581 pKa = 4.29 RR582 pKa = 11.84 RR583 pKa = 11.84 KK584 pKa = 9.34 MLTKK588 pKa = 10.41 RR589 pKa = 11.84 AAKK592 pKa = 9.77 RR593 pKa = 11.84 IKK595 pKa = 10.36 KK596 pKa = 10.16 IIDD599 pKa = 3.52 SEE601 pKa = 4.55 KK602 pKa = 10.64 SLSSTTGQSQLEE614 pKa = 4.14 IPLQQEE620 pKa = 4.14 AEE622 pKa = 3.99 TDD624 pKa = 3.11 ISSEE628 pKa = 4.02 EE629 pKa = 4.01 EE630 pKa = 4.08 AEE632 pKa = 4.69 KK633 pKa = 9.94 EE634 pKa = 4.22 TLLLLLRR641 pKa = 11.84 QQRR644 pKa = 11.84 LKK646 pKa = 9.2 QAKK649 pKa = 8.33 YY650 pKa = 10.03 KK651 pKa = 10.37 QRR653 pKa = 11.84 LLQLIEE659 pKa = 3.87 QLQTLEE665 pKa = 4.0
Molecular weight: 79.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.809
IPC2_protein 9.092
IPC_protein 8.975
Toseland 9.472
ProMoST 9.341
Dawson 9.809
Bjellqvist 9.545
Wikipedia 10.014
Rodwell 10.028
Grimsley 9.911
Solomon 9.823
Lehninger 9.765
Nozaki 9.531
DTASelect 9.516
Thurlkill 9.619
EMBOSS 9.926
Sillero 9.721
Patrickios 4.507
IPC_peptide 9.809
IPC2_peptide 8.156
IPC2.peptide.svr19 7.935
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
765
100
665
382.5
45.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.137 ± 0.497
1.438 ± 1.119
5.229 ± 2.084
6.144 ± 2.558
4.314 ± 0.137
3.66 ± 0.585
2.353 ± 0.154
6.667 ± 1.165
9.02 ± 0.009
8.889 ± 0.485
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.961 ± 0.42
4.444 ± 0.194
6.797 ± 1.222
6.275 ± 0.12
5.621 ± 1.145
4.967 ± 0.423
8.758 ± 0.979
1.699 ± 0.131
1.83 ± 0.363
6.797 ± 2.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here