Eptesipox virus
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 179 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220T6E6|A0A220T6E6_9POXV Virion core protein OS=Eptesipox virus OX=1329402 GN=EPTV-WA-084 PE=3 SV=1
MM1 pKa = 7.8 GITHH5 pKa = 6.07 QMDD8 pKa = 3.19 VHH10 pKa = 6.32 VCSEE14 pKa = 4.17 NISIVGAAPKK24 pKa = 10.37 KK25 pKa = 10.3 GDD27 pKa = 3.61 YY28 pKa = 10.69 FGMKK32 pKa = 9.45 MNITSTEE39 pKa = 3.72 EE40 pKa = 3.94 HH41 pKa = 7.13 EE42 pKa = 4.24 ISLVIIIYY50 pKa = 9.44 PDD52 pKa = 3.04 WSEE55 pKa = 4.21 VPGIRR60 pKa = 11.84 PFIVTVNDD68 pKa = 2.88 VKK70 pKa = 11.08 IPVKK74 pKa = 10.1 RR75 pKa = 11.84 AYY77 pKa = 9.59 EE78 pKa = 3.95 SLYY81 pKa = 10.97 KK82 pKa = 10.84 VIFTCKK88 pKa = 9.84 FIVMGNVYY96 pKa = 8.68 VTMYY100 pKa = 10.57 SKK102 pKa = 11.15 NDD104 pKa = 3.1 EE105 pKa = 4.18 EE106 pKa = 4.18 YY107 pKa = 10.78 LYY109 pKa = 10.83 DD110 pKa = 3.98 KK111 pKa = 10.75 RR112 pKa = 11.84 CAEE115 pKa = 3.7 IDD117 pKa = 3.34 INIDD121 pKa = 3.28 EE122 pKa = 5.01 KK123 pKa = 11.18 EE124 pKa = 4.05 FNLISEE130 pKa = 5.42 GYY132 pKa = 7.87 TYY134 pKa = 10.9 AYY136 pKa = 9.89 AYY138 pKa = 10.9 SPIDD142 pKa = 3.5 EE143 pKa = 4.4 EE144 pKa = 4.44 NKK146 pKa = 10.37 KK147 pKa = 10.53 EE148 pKa = 4.25 CLTALNEE155 pKa = 4.46 SKK157 pKa = 9.47 VTGDD161 pKa = 4.74 YY162 pKa = 11.34 DD163 pKa = 6.2 DD164 pKa = 6.16 DD165 pKa = 6.98 DD166 pKa = 6.19 NDD168 pKa = 5.71 DD169 pKa = 5.33 DD170 pKa = 6.03 INDD173 pKa = 3.97 IGNIVYY179 pKa = 10.22 KK180 pKa = 9.8 EE181 pKa = 3.82 QEE183 pKa = 3.84 FEE185 pKa = 4.11 YY186 pKa = 10.95 DD187 pKa = 3.02 SDD189 pKa = 4.83 EE190 pKa = 4.55 NDD192 pKa = 3.54 FDD194 pKa = 4.03 NTTDD198 pKa = 3.45 SDD200 pKa = 4.0 NYY202 pKa = 10.99 DD203 pKa = 3.1
Molecular weight: 23.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 4.012
IPC_protein 3.999
Toseland 3.795
ProMoST 4.113
Dawson 3.973
Bjellqvist 4.151
Wikipedia 3.897
Rodwell 3.834
Grimsley 3.706
Solomon 3.973
Lehninger 3.935
Nozaki 4.088
DTASelect 4.304
Thurlkill 3.834
EMBOSS 3.897
Sillero 4.126
Patrickios 1.011
IPC_peptide 3.973
IPC2_peptide 4.101
IPC2.peptide.svr19 4.011
Protein with the highest isoelectric point:
>tr|A0A220T696|A0A220T696_9POXV Protein F16 OS=Eptesipox virus OX=1329402 GN=EPTV-WA-033 PE=3 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.34 LFKK5 pKa = 10.51 FFKK8 pKa = 9.28 KK9 pKa = 6.45 THH11 pKa = 5.51 KK12 pKa = 10.22 PKK14 pKa = 10.21 RR15 pKa = 11.84 QPSKK19 pKa = 9.83 WISSLDD25 pKa = 3.5 VRR27 pKa = 11.84 SGNIVITDD35 pKa = 3.74 SKK37 pKa = 11.53 SCMDD41 pKa = 4.27 LSYY44 pKa = 11.39 INVNKK49 pKa = 10.0 EE50 pKa = 3.79 LNQAATPNLEE60 pKa = 3.88 RR61 pKa = 11.84 RR62 pKa = 11.84 RR63 pKa = 11.84 KK64 pKa = 9.51 IFF66 pKa = 3.46
Molecular weight: 7.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.189
IPC2_protein 9.619
IPC_protein 9.692
Toseland 10.774
ProMoST 10.233
Dawson 10.833
Bjellqvist 10.409
Wikipedia 10.935
Rodwell 11.447
Grimsley 10.847
Solomon 10.877
Lehninger 10.877
Nozaki 10.745
DTASelect 10.409
Thurlkill 10.745
EMBOSS 11.14
Sillero 10.745
Patrickios 11.199
IPC_peptide 10.891
IPC2_peptide 8.916
IPC2.peptide.svr19 8.671
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
179
0
179
52572
53
1285
293.7
34.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.355 ± 0.104
2.231 ± 0.096
5.609 ± 0.132
5.343 ± 0.194
5.206 ± 0.13
3.203 ± 0.11
1.864 ± 0.073
10.816 ± 0.205
9.127 ± 0.138
9.001 ± 0.141
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.385 ± 0.079
8.759 ± 0.225
2.884 ± 0.113
2.359 ± 0.076
2.726 ± 0.122
7.213 ± 0.148
6.264 ± 0.139
5.377 ± 0.114
0.67 ± 0.039
5.608 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here