Moraxella phage Mcat25

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R6PEW1|A0A0R6PEW1_9CAUD Uncharacterized protein OS=Moraxella phage Mcat25 OX=1647541 PE=4 SV=1
MM1 pKa = 7.49VKK3 pKa = 9.41HH4 pKa = 5.63QCRR7 pKa = 11.84IDD9 pKa = 4.23HH10 pKa = 7.34DD11 pKa = 5.06DD12 pKa = 3.96EE13 pKa = 7.53DD14 pKa = 5.13DD15 pKa = 4.29LLMRR19 pKa = 11.84YY20 pKa = 9.75LNAALIHH27 pKa = 6.2ASNYY31 pKa = 9.48IDD33 pKa = 4.47GLLDD37 pKa = 3.61EE38 pKa = 5.83SNLAVQQAVLLLVGHH53 pKa = 7.35WYY55 pKa = 10.33DD56 pKa = 4.08NRR58 pKa = 11.84EE59 pKa = 4.03AVNNDD64 pKa = 2.91YY65 pKa = 8.08QTPQSIPFGFEE76 pKa = 3.73ALLQPYY82 pKa = 10.05RR83 pKa = 11.84NLGVV87 pKa = 3.22

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R6PJL6|A0A0R6PJL6_9CAUD Coat protein OS=Moraxella phage Mcat25 OX=1647541 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84RR3 pKa = 11.84CTALFFVLDD12 pKa = 4.15ITPLSYY18 pKa = 11.28KK19 pKa = 10.49NIRR22 pKa = 11.84NYY24 pKa = 10.75KK25 pKa = 10.62LNDD28 pKa = 3.41TKK30 pKa = 11.35NQLKK34 pKa = 10.34NKK36 pKa = 9.71HH37 pKa = 6.32SNLL40 pKa = 3.6

Molecular weight: 4.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12284

37

1652

240.9

26.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.515 ± 0.637

1.05 ± 0.196

6.154 ± 0.33

5.617 ± 0.412

3.411 ± 0.254

6.716 ± 0.441

2.499 ± 0.204

6.521 ± 0.246

6.716 ± 0.255

8.833 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.491 ± 0.312

5.568 ± 0.445

3.15 ± 0.289

4.722 ± 0.289

4.363 ± 0.299

6.92 ± 0.498

6.659 ± 0.576

5.609 ± 0.208

1.164 ± 0.123

3.321 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski