Streptococcus satellite phage Javan194
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZGR6|A0A4D5ZGR6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan194 OX=2558559 GN=JavanS194_0013 PE=4 SV=1
MM1 pKa = 7.39 LVTYY5 pKa = 8.27 PALFYY10 pKa = 11.35 YY11 pKa = 10.68 DD12 pKa = 4.04 DD13 pKa = 4.01 TDD15 pKa = 3.86 GANAPYY21 pKa = 10.03 FVTFPDD27 pKa = 4.21 FEE29 pKa = 4.62 YY30 pKa = 10.92 SATQGEE36 pKa = 4.55 DD37 pKa = 3.0 MADD40 pKa = 3.52 AMAMASDD47 pKa = 3.34 WLGIHH52 pKa = 6.56 LADD55 pKa = 4.36 YY56 pKa = 10.61 IEE58 pKa = 4.46 NGRR61 pKa = 11.84 DD62 pKa = 3.05 IPTPTSINALSLADD76 pKa = 3.93 NNPFRR81 pKa = 11.84 DD82 pKa = 3.87 DD83 pKa = 3.58 KK84 pKa = 11.4 DD85 pKa = 3.43 IEE87 pKa = 4.32 LVYY90 pKa = 10.75 DD91 pKa = 3.98 PSKK94 pKa = 11.15 SFVSMVMVDD103 pKa = 3.2 VAEE106 pKa = 4.14 YY107 pKa = 10.49 LGSQEE112 pKa = 4.15 PVKK115 pKa = 10.18 KK116 pKa = 9.12 TLTIPRR122 pKa = 11.84 WADD125 pKa = 3.13 TLGRR129 pKa = 11.84 EE130 pKa = 4.01 LGLNFSQTLTDD141 pKa = 5.45 AIADD145 pKa = 4.03 KK146 pKa = 10.56 KK147 pKa = 10.31 IHH149 pKa = 5.92 AA150 pKa = 5.0
Molecular weight: 16.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.101
IPC2_protein 3.986
IPC_protein 3.986
Toseland 3.757
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.808
Grimsley 3.668
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.368
Thurlkill 3.821
EMBOSS 3.948
Sillero 4.101
Patrickios 3.312
IPC_peptide 3.973
IPC2_peptide 4.075
IPC2.peptide.svr19 4.022
Protein with the highest isoelectric point:
>tr|A0A4D5ZFI3|A0A4D5ZFI3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan194 OX=2558559 GN=JavanS194_0020 PE=4 SV=1
MM1 pKa = 7.28 AGKK4 pKa = 10.07 RR5 pKa = 11.84 LKK7 pKa = 10.8 HH8 pKa = 5.98 PALKK12 pKa = 10.34 GLRR15 pKa = 11.84 IGQKK19 pKa = 10.55 NIIKK23 pKa = 10.15 DD24 pKa = 3.7 RR25 pKa = 11.84 QPTIEE30 pKa = 4.32 QIKK33 pKa = 8.53 EE34 pKa = 3.65 QQRR37 pKa = 11.84 LKK39 pKa = 10.73 KK40 pKa = 10.24 LKK42 pKa = 10.01 KK43 pKa = 9.67 KK44 pKa = 9.88 RR45 pKa = 11.84 RR46 pKa = 11.84 KK47 pKa = 9.34
Molecular weight: 5.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.169
IPC2_protein 10.248
IPC_protein 11.55
Toseland 11.842
ProMoST 12.223
Dawson 11.857
Bjellqvist 11.769
Wikipedia 12.252
Rodwell 12.047
Grimsley 11.886
Solomon 12.266
Lehninger 12.179
Nozaki 11.828
DTASelect 11.769
Thurlkill 11.828
EMBOSS 12.31
Sillero 11.828
Patrickios 11.769
IPC_peptide 12.266
IPC2_peptide 11.199
IPC2.peptide.svr19 9.037
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21
0
21
3080
44
388
146.7
16.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.266 ± 0.467
0.487 ± 0.201
5.552 ± 0.527
7.727 ± 0.579
3.701 ± 0.289
4.675 ± 0.478
1.591 ± 0.289
6.948 ± 0.323
9.513 ± 0.591
10.584 ± 0.573
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.792 ± 0.247
5.584 ± 0.524
3.636 ± 0.343
5.292 ± 0.486
4.773 ± 0.33
5.39 ± 0.378
5.909 ± 0.281
4.61 ± 0.428
0.909 ± 0.214
4.058 ± 0.467
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here