Amino acid dipepetide frequency for Gordonia phage EnalisNailo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.652AlaAla: 16.652 ± 1.67
1.022AlaCys: 1.022 ± 0.301
7.815AlaAsp: 7.815 ± 0.597
8.656AlaGlu: 8.656 ± 0.783
3.126AlaPhe: 3.126 ± 0.561
10.28AlaGly: 10.28 ± 0.783
2.765AlaHis: 2.765 ± 0.49
4.809AlaIle: 4.809 ± 0.542
4.268AlaLys: 4.268 ± 0.554
7.815AlaLeu: 7.815 ± 0.761
3.907AlaMet: 3.907 ± 0.769
3.787AlaAsn: 3.787 ± 0.761
4.749AlaPro: 4.749 ± 0.587
4.388AlaGln: 4.388 ± 0.596
8.296AlaArg: 8.296 ± 0.722
6.552AlaSer: 6.552 ± 0.74
7.454AlaThr: 7.454 ± 0.601
7.995AlaVal: 7.995 ± 0.812
2.585AlaTrp: 2.585 ± 0.368
2.525AlaTyr: 2.525 ± 0.422
0.0AlaXaa: 0.0 ± 0.0
Cys
0.962CysAla: 0.962 ± 0.253
0.12CysCys: 0.12 ± 0.082
0.902CysAsp: 0.902 ± 0.268
0.421CysGlu: 0.421 ± 0.155
0.12CysPhe: 0.12 ± 0.084
1.503CysGly: 1.503 ± 0.411
0.301CysHis: 0.301 ± 0.147
0.18CysIle: 0.18 ± 0.098
0.12CysLys: 0.12 ± 0.088
0.361CysLeu: 0.361 ± 0.169
0.18CysMet: 0.18 ± 0.115
0.12CysAsn: 0.12 ± 0.089
0.24CysPro: 0.24 ± 0.156
0.541CysGln: 0.541 ± 0.158
1.503CysArg: 1.503 ± 0.385
0.421CysSer: 0.421 ± 0.161
0.601CysThr: 0.601 ± 0.17
0.481CysVal: 0.481 ± 0.171
0.18CysTrp: 0.18 ± 0.107
0.361CysTyr: 0.361 ± 0.142
0.0CysXaa: 0.0 ± 0.0
Asp
7.875AspAla: 7.875 ± 0.672
0.481AspCys: 0.481 ± 0.173
4.509AspAsp: 4.509 ± 0.688
4.088AspGlu: 4.088 ± 0.511
1.503AspPhe: 1.503 ± 0.256
7.214AspGly: 7.214 ± 0.628
1.984AspHis: 1.984 ± 0.372
2.765AspIle: 2.765 ± 0.316
1.323AspLys: 1.323 ± 0.274
7.454AspLeu: 7.454 ± 0.67
1.082AspMet: 1.082 ± 0.285
1.623AspAsn: 1.623 ± 0.354
4.929AspPro: 4.929 ± 0.533
2.585AspGln: 2.585 ± 0.468
5.771AspArg: 5.771 ± 0.491
2.104AspSer: 2.104 ± 0.38
3.066AspThr: 3.066 ± 0.517
4.148AspVal: 4.148 ± 0.436
1.443AspTrp: 1.443 ± 0.293
1.323AspTyr: 1.323 ± 0.329
0.0AspXaa: 0.0 ± 0.0
Glu
5.651GluAla: 5.651 ± 0.588
0.541GluCys: 0.541 ± 0.215
2.525GluAsp: 2.525 ± 0.427
3.066GluGlu: 3.066 ± 0.439
2.344GluPhe: 2.344 ± 0.494
3.126GluGly: 3.126 ± 0.446
1.623GluHis: 1.623 ± 0.309
3.126GluIle: 3.126 ± 0.566
2.284GluLys: 2.284 ± 0.362
5.29GluLeu: 5.29 ± 0.643
0.902GluMet: 0.902 ± 0.183
2.104GluAsn: 2.104 ± 0.305
2.885GluPro: 2.885 ± 0.368
1.924GluGln: 1.924 ± 0.356
3.787GluArg: 3.787 ± 0.523
3.847GluSer: 3.847 ± 0.584
3.006GluThr: 3.006 ± 0.373
5.23GluVal: 5.23 ± 0.703
1.262GluTrp: 1.262 ± 0.284
1.503GluTyr: 1.503 ± 0.298
0.0GluXaa: 0.0 ± 0.0
Phe
2.465PheAla: 2.465 ± 0.394
0.301PheCys: 0.301 ± 0.153
2.164PheAsp: 2.164 ± 0.337
1.623PheGlu: 1.623 ± 0.361
0.301PhePhe: 0.301 ± 0.156
2.825PheGly: 2.825 ± 0.539
0.541PheHis: 0.541 ± 0.174
1.383PheIle: 1.383 ± 0.183
0.661PheLys: 0.661 ± 0.152
2.044PheLeu: 2.044 ± 0.316
0.361PheMet: 0.361 ± 0.169
0.781PheAsn: 0.781 ± 0.184
1.383PhePro: 1.383 ± 0.326
0.842PheGln: 0.842 ± 0.247
1.984PheArg: 1.984 ± 0.284
2.044PheSer: 2.044 ± 0.385
2.044PheThr: 2.044 ± 0.361
2.164PheVal: 2.164 ± 0.341
0.481PheTrp: 0.481 ± 0.162
0.541PheTyr: 0.541 ± 0.136
0.0PheXaa: 0.0 ± 0.0
Gly
9.618GlyAla: 9.618 ± 1.082
0.661GlyCys: 0.661 ± 0.293
5.47GlyAsp: 5.47 ± 0.705
4.088GlyGlu: 4.088 ± 0.524
2.284GlyPhe: 2.284 ± 0.371
9.378GlyGly: 9.378 ± 1.238
2.104GlyHis: 2.104 ± 0.399
3.847GlyIle: 3.847 ± 0.493
3.126GlyLys: 3.126 ± 0.421
5.29GlyLeu: 5.29 ± 0.68
1.503GlyMet: 1.503 ± 0.261
3.246GlyAsn: 3.246 ± 0.515
4.208GlyPro: 4.208 ± 0.451
3.607GlyGln: 3.607 ± 0.51
7.334GlyArg: 7.334 ± 0.719
4.569GlySer: 4.569 ± 0.736
6.192GlyThr: 6.192 ± 0.917
5.711GlyVal: 5.711 ± 0.577
1.924GlyTrp: 1.924 ± 0.326
1.443GlyTyr: 1.443 ± 0.261
0.0GlyXaa: 0.0 ± 0.0
His
2.104HisAla: 2.104 ± 0.356
0.481HisCys: 0.481 ± 0.181
1.082HisAsp: 1.082 ± 0.218
1.082HisGlu: 1.082 ± 0.228
0.301HisPhe: 0.301 ± 0.137
2.164HisGly: 2.164 ± 0.414
0.842HisHis: 0.842 ± 0.219
0.902HisIle: 0.902 ± 0.24
0.301HisLys: 0.301 ± 0.104
2.044HisLeu: 2.044 ± 0.374
0.18HisMet: 0.18 ± 0.104
0.781HisAsn: 0.781 ± 0.199
2.104HisPro: 2.104 ± 0.515
0.902HisGln: 0.902 ± 0.361
1.803HisArg: 1.803 ± 0.402
1.022HisSer: 1.022 ± 0.3
1.683HisThr: 1.683 ± 0.25
1.142HisVal: 1.142 ± 0.28
0.481HisTrp: 0.481 ± 0.194
0.902HisTyr: 0.902 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
6.372IleAla: 6.372 ± 0.637
0.18IleCys: 0.18 ± 0.119
3.787IleAsp: 3.787 ± 0.473
3.306IleGlu: 3.306 ± 0.441
0.721IlePhe: 0.721 ± 0.228
3.547IleGly: 3.547 ± 0.461
0.721IleHis: 0.721 ± 0.223
1.503IleIle: 1.503 ± 0.325
1.022IleLys: 1.022 ± 0.312
3.246IleLeu: 3.246 ± 0.461
0.24IleMet: 0.24 ± 0.118
1.503IleAsn: 1.503 ± 0.268
2.885IlePro: 2.885 ± 0.403
1.803IleGln: 1.803 ± 0.331
3.667IleArg: 3.667 ± 0.451
2.224IleSer: 2.224 ± 0.294
3.607IleThr: 3.607 ± 0.481
2.525IleVal: 2.525 ± 0.425
0.721IleTrp: 0.721 ± 0.205
1.082IleTyr: 1.082 ± 0.25
0.0IleXaa: 0.0 ± 0.0
Lys
3.667LysAla: 3.667 ± 0.472
0.18LysCys: 0.18 ± 0.093
1.683LysAsp: 1.683 ± 0.329
1.022LysGlu: 1.022 ± 0.208
0.842LysPhe: 0.842 ± 0.27
2.104LysGly: 2.104 ± 0.421
0.541LysHis: 0.541 ± 0.196
1.383LysIle: 1.383 ± 0.318
1.202LysLys: 1.202 ± 0.39
2.885LysLeu: 2.885 ± 0.48
0.541LysMet: 0.541 ± 0.155
1.262LysAsn: 1.262 ± 0.322
2.224LysPro: 2.224 ± 0.494
0.902LysGln: 0.902 ± 0.249
3.186LysArg: 3.186 ± 0.402
2.044LysSer: 2.044 ± 0.376
2.705LysThr: 2.705 ± 0.447
2.224LysVal: 2.224 ± 0.365
0.361LysTrp: 0.361 ± 0.156
0.601LysTyr: 0.601 ± 0.204
0.0LysXaa: 0.0 ± 0.0
Leu
9.919LeuAla: 9.919 ± 0.854
0.842LeuCys: 0.842 ± 0.269
6.192LeuAsp: 6.192 ± 0.715
4.569LeuGlu: 4.569 ± 0.561
2.525LeuPhe: 2.525 ± 0.361
5.771LeuGly: 5.771 ± 0.799
1.142LeuHis: 1.142 ± 0.24
3.607LeuIle: 3.607 ± 0.558
2.284LeuLys: 2.284 ± 0.413
4.148LeuLeu: 4.148 ± 0.461
1.623LeuMet: 1.623 ± 0.277
2.405LeuAsn: 2.405 ± 0.447
5.29LeuPro: 5.29 ± 0.549
2.765LeuGln: 2.765 ± 0.449
6.132LeuArg: 6.132 ± 0.837
3.787LeuSer: 3.787 ± 0.483
6.192LeuThr: 6.192 ± 0.675
4.328LeuVal: 4.328 ± 0.641
1.623LeuTrp: 1.623 ± 0.409
1.323LeuTyr: 1.323 ± 0.227
0.0LeuXaa: 0.0 ± 0.0
Met
2.405MetAla: 2.405 ± 0.378
0.361MetCys: 0.361 ± 0.152
1.022MetAsp: 1.022 ± 0.248
1.022MetGlu: 1.022 ± 0.233
0.421MetPhe: 0.421 ± 0.139
1.503MetGly: 1.503 ± 0.365
0.301MetHis: 0.301 ± 0.129
0.421MetIle: 0.421 ± 0.127
0.902MetLys: 0.902 ± 0.22
1.623MetLeu: 1.623 ± 0.275
0.301MetMet: 0.301 ± 0.184
0.661MetAsn: 0.661 ± 0.177
0.842MetPro: 0.842 ± 0.219
0.902MetGln: 0.902 ± 0.404
1.563MetArg: 1.563 ± 0.314
1.864MetSer: 1.864 ± 0.355
3.066MetThr: 3.066 ± 0.397
1.082MetVal: 1.082 ± 0.223
0.661MetTrp: 0.661 ± 0.182
0.18MetTyr: 0.18 ± 0.094
0.0MetXaa: 0.0 ± 0.0
Asn
4.088AsnAla: 4.088 ± 0.735
0.18AsnCys: 0.18 ± 0.11
2.044AsnAsp: 2.044 ± 0.35
1.262AsnGlu: 1.262 ± 0.212
0.962AsnPhe: 0.962 ± 0.308
3.366AsnGly: 3.366 ± 0.524
0.842AsnHis: 0.842 ± 0.324
1.202AsnIle: 1.202 ± 0.293
0.962AsnLys: 0.962 ± 0.225
2.405AsnLeu: 2.405 ± 0.514
0.421AsnMet: 0.421 ± 0.14
0.601AsnAsn: 0.601 ± 0.224
2.284AsnPro: 2.284 ± 0.342
1.623AsnGln: 1.623 ± 0.348
2.044AsnArg: 2.044 ± 0.348
2.044AsnSer: 2.044 ± 0.392
1.924AsnThr: 1.924 ± 0.318
2.044AsnVal: 2.044 ± 0.317
0.541AsnTrp: 0.541 ± 0.184
0.541AsnTyr: 0.541 ± 0.237
0.0AsnXaa: 0.0 ± 0.0
Pro
5.951ProAla: 5.951 ± 0.624
0.481ProCys: 0.481 ± 0.176
4.809ProAsp: 4.809 ± 0.644
4.088ProGlu: 4.088 ± 0.426
1.323ProPhe: 1.323 ± 0.212
5.29ProGly: 5.29 ± 0.557
1.563ProHis: 1.563 ± 0.402
2.284ProIle: 2.284 ± 0.346
1.984ProLys: 1.984 ± 0.328
4.208ProLeu: 4.208 ± 0.562
0.842ProMet: 0.842 ± 0.217
1.924ProAsn: 1.924 ± 0.337
3.427ProPro: 3.427 ± 0.612
1.503ProGln: 1.503 ± 0.29
4.509ProArg: 4.509 ± 0.792
2.765ProSer: 2.765 ± 0.427
3.487ProThr: 3.487 ± 0.498
3.787ProVal: 3.787 ± 0.671
1.443ProTrp: 1.443 ± 0.355
1.383ProTyr: 1.383 ± 0.311
0.0ProXaa: 0.0 ± 0.0
Gln
4.388GlnAla: 4.388 ± 0.688
0.301GlnCys: 0.301 ± 0.138
2.044GlnAsp: 2.044 ± 0.3
1.082GlnGlu: 1.082 ± 0.228
1.443GlnPhe: 1.443 ± 0.322
2.465GlnGly: 2.465 ± 0.567
0.902GlnHis: 0.902 ± 0.202
2.344GlnIle: 2.344 ± 0.379
1.142GlnLys: 1.142 ± 0.354
3.126GlnLeu: 3.126 ± 0.562
1.022GlnMet: 1.022 ± 0.252
0.842GlnAsn: 0.842 ± 0.285
1.683GlnPro: 1.683 ± 0.267
2.344GlnGln: 2.344 ± 0.786
3.126GlnArg: 3.126 ± 0.464
2.465GlnSer: 2.465 ± 0.471
2.164GlnThr: 2.164 ± 0.372
2.344GlnVal: 2.344 ± 0.49
1.022GlnTrp: 1.022 ± 0.236
0.962GlnTyr: 0.962 ± 0.238
0.0GlnXaa: 0.0 ± 0.0
Arg
8.536ArgAla: 8.536 ± 0.715
1.202ArgCys: 1.202 ± 0.314
4.148ArgAsp: 4.148 ± 0.508
4.268ArgGlu: 4.268 ± 0.576
2.705ArgPhe: 2.705 ± 0.335
4.509ArgGly: 4.509 ± 0.476
1.743ArgHis: 1.743 ± 0.428
4.088ArgIle: 4.088 ± 0.559
3.667ArgLys: 3.667 ± 0.46
5.531ArgLeu: 5.531 ± 0.6
2.585ArgMet: 2.585 ± 0.351
3.066ArgAsn: 3.066 ± 0.446
4.989ArgPro: 4.989 ± 0.683
2.705ArgGln: 2.705 ± 0.488
6.913ArgArg: 6.913 ± 0.83
3.787ArgSer: 3.787 ± 0.495
4.689ArgThr: 4.689 ± 0.629
5.23ArgVal: 5.23 ± 0.546
1.864ArgTrp: 1.864 ± 0.376
2.344ArgTyr: 2.344 ± 0.418
0.0ArgXaa: 0.0 ± 0.0
Ser
6.913SerAla: 6.913 ± 0.95
0.12SerCys: 0.12 ± 0.093
3.847SerAsp: 3.847 ± 0.359
3.186SerGlu: 3.186 ± 0.426
1.262SerPhe: 1.262 ± 0.265
6.132SerGly: 6.132 ± 0.929
0.661SerHis: 0.661 ± 0.185
2.104SerIle: 2.104 ± 0.337
1.683SerLys: 1.683 ± 0.369
4.208SerLeu: 4.208 ± 0.512
1.563SerMet: 1.563 ± 0.299
1.323SerAsn: 1.323 ± 0.287
2.645SerPro: 2.645 ± 0.318
2.284SerGln: 2.284 ± 0.423
3.066SerArg: 3.066 ± 0.416
3.246SerSer: 3.246 ± 0.483
4.328SerThr: 4.328 ± 0.635
3.487SerVal: 3.487 ± 0.443
1.142SerTrp: 1.142 ± 0.206
1.202SerTyr: 1.202 ± 0.252
0.0SerXaa: 0.0 ± 0.0
Thr
9.258ThrAla: 9.258 ± 0.787
0.902ThrCys: 0.902 ± 0.273
4.689ThrAsp: 4.689 ± 0.375
2.885ThrGlu: 2.885 ± 0.506
1.803ThrPhe: 1.803 ± 0.317
5.951ThrGly: 5.951 ± 0.617
1.563ThrHis: 1.563 ± 0.354
4.208ThrIle: 4.208 ± 0.498
1.623ThrLys: 1.623 ± 0.263
6.192ThrLeu: 6.192 ± 0.617
1.082ThrMet: 1.082 ± 0.336
1.803ThrAsn: 1.803 ± 0.37
4.629ThrPro: 4.629 ± 0.671
1.683ThrGln: 1.683 ± 0.352
5.29ThrArg: 5.29 ± 0.742
3.186ThrSer: 3.186 ± 0.506
5.29ThrThr: 5.29 ± 0.648
5.711ThrVal: 5.711 ± 0.808
1.323ThrTrp: 1.323 ± 0.262
1.142ThrTyr: 1.142 ± 0.281
0.0ThrXaa: 0.0 ± 0.0
Val
7.514ValAla: 7.514 ± 0.629
0.842ValCys: 0.842 ± 0.236
5.831ValAsp: 5.831 ± 0.613
4.148ValGlu: 4.148 ± 0.724
1.743ValPhe: 1.743 ± 0.325
5.41ValGly: 5.41 ± 0.622
1.262ValHis: 1.262 ± 0.277
2.885ValIle: 2.885 ± 0.463
1.924ValLys: 1.924 ± 0.382
4.328ValLeu: 4.328 ± 0.564
1.323ValMet: 1.323 ± 0.308
2.405ValAsn: 2.405 ± 0.356
4.088ValPro: 4.088 ± 0.437
2.224ValGln: 2.224 ± 0.389
4.809ValArg: 4.809 ± 0.585
4.088ValSer: 4.088 ± 0.53
5.951ValThr: 5.951 ± 0.653
5.05ValVal: 5.05 ± 0.672
1.262ValTrp: 1.262 ± 0.309
0.842ValTyr: 0.842 ± 0.181
0.0ValXaa: 0.0 ± 0.0
Trp
2.705TrpAla: 2.705 ± 0.438
0.18TrpCys: 0.18 ± 0.103
1.202TrpAsp: 1.202 ± 0.267
1.082TrpGlu: 1.082 ± 0.24
0.421TrpPhe: 0.421 ± 0.161
1.443TrpGly: 1.443 ± 0.242
0.601TrpHis: 0.601 ± 0.211
0.781TrpIle: 0.781 ± 0.223
0.541TrpLys: 0.541 ± 0.165
2.344TrpLeu: 2.344 ± 0.386
0.842TrpMet: 0.842 ± 0.196
0.661TrpAsn: 0.661 ± 0.17
0.661TrpPro: 0.661 ± 0.202
0.962TrpGln: 0.962 ± 0.252
1.864TrpArg: 1.864 ± 0.288
1.082TrpSer: 1.082 ± 0.257
1.323TrpThr: 1.323 ± 0.267
1.683TrpVal: 1.683 ± 0.28
0.601TrpTrp: 0.601 ± 0.243
0.361TrpTyr: 0.361 ± 0.102
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.405TyrAla: 2.405 ± 0.333
0.24TyrCys: 0.24 ± 0.116
1.503TyrAsp: 1.503 ± 0.279
1.082TyrGlu: 1.082 ± 0.191
0.781TyrPhe: 0.781 ± 0.184
1.623TyrGly: 1.623 ± 0.261
0.301TyrHis: 0.301 ± 0.154
0.842TyrIle: 0.842 ± 0.226
0.541TyrLys: 0.541 ± 0.187
1.924TyrLeu: 1.924 ± 0.302
0.541TyrMet: 0.541 ± 0.147
0.481TyrAsn: 0.481 ± 0.146
0.902TyrPro: 0.902 ± 0.27
0.781TyrGln: 0.781 ± 0.197
1.924TyrArg: 1.924 ± 0.372
1.262TyrSer: 1.262 ± 0.297
1.443TyrThr: 1.443 ± 0.369
1.563TyrVal: 1.563 ± 0.326
0.421TyrTrp: 0.421 ± 0.171
0.361TyrTyr: 0.361 ± 0.14
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (16636 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski