Amino acid dipepetide frequency for Vibrio phage vB_VnaS-AQKL99

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.813AlaAla: 6.813 ± 1.155
1.145AlaCys: 1.145 ± 0.263
5.124AlaAsp: 5.124 ± 0.511
6.33AlaGlu: 6.33 ± 0.813
3.256AlaPhe: 3.256 ± 0.491
4.28AlaGly: 4.28 ± 0.518
1.748AlaHis: 1.748 ± 0.308
4.341AlaIle: 4.341 ± 0.538
5.607AlaLys: 5.607 ± 0.631
6.692AlaLeu: 6.692 ± 0.549
2.472AlaMet: 2.472 ± 0.357
3.858AlaAsn: 3.858 ± 0.484
3.195AlaPro: 3.195 ± 0.706
3.436AlaGln: 3.436 ± 0.517
4.522AlaArg: 4.522 ± 0.565
4.522AlaSer: 4.522 ± 0.57
5.969AlaThr: 5.969 ± 0.576
5.547AlaVal: 5.547 ± 0.547
0.904AlaTrp: 0.904 ± 0.249
3.135AlaTyr: 3.135 ± 0.36
0.0AlaXaa: 0.0 ± 0.0
Cys
1.145CysAla: 1.145 ± 0.319
0.181CysCys: 0.181 ± 0.101
1.266CysAsp: 1.266 ± 0.251
1.085CysGlu: 1.085 ± 0.291
0.784CysPhe: 0.784 ± 0.248
1.085CysGly: 1.085 ± 0.351
0.603CysHis: 0.603 ± 0.207
0.603CysIle: 0.603 ± 0.208
0.723CysLys: 0.723 ± 0.236
1.145CysLeu: 1.145 ± 0.233
0.482CysMet: 0.482 ± 0.175
0.723CysAsn: 0.723 ± 0.215
0.663CysPro: 0.663 ± 0.193
0.543CysGln: 0.543 ± 0.194
0.844CysArg: 0.844 ± 0.234
0.482CysSer: 0.482 ± 0.151
0.482CysThr: 0.482 ± 0.162
0.723CysVal: 0.723 ± 0.154
0.06CysTrp: 0.06 ± 0.056
0.241CysTyr: 0.241 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
5.667AspAla: 5.667 ± 0.571
1.025AspCys: 1.025 ± 0.233
3.617AspAsp: 3.617 ± 0.494
4.28AspGlu: 4.28 ± 0.565
2.291AspPhe: 2.291 ± 0.348
4.522AspGly: 4.522 ± 0.569
1.748AspHis: 1.748 ± 0.247
3.436AspIle: 3.436 ± 0.376
3.678AspLys: 3.678 ± 0.415
4.763AspLeu: 4.763 ± 0.471
1.567AspMet: 1.567 ± 0.303
1.809AspAsn: 1.809 ± 0.283
2.231AspPro: 2.231 ± 0.444
1.266AspGln: 1.266 ± 0.237
2.592AspArg: 2.592 ± 0.401
4.1AspSer: 4.1 ± 0.551
3.376AspThr: 3.376 ± 0.538
4.16AspVal: 4.16 ± 0.515
0.965AspTrp: 0.965 ± 0.24
2.773AspTyr: 2.773 ± 0.376
0.0AspXaa: 0.0 ± 0.0
Glu
5.426GluAla: 5.426 ± 0.723
1.326GluCys: 1.326 ± 0.256
2.834GluAsp: 2.834 ± 0.482
3.256GluGlu: 3.256 ± 0.46
2.472GluPhe: 2.472 ± 0.405
4.522GluGly: 4.522 ± 0.477
1.748GluHis: 1.748 ± 0.345
3.617GluIle: 3.617 ± 0.424
3.075GluLys: 3.075 ± 0.52
7.596GluLeu: 7.596 ± 0.653
2.17GluMet: 2.17 ± 0.352
2.592GluAsn: 2.592 ± 0.425
2.05GluPro: 2.05 ± 0.328
3.858GluGln: 3.858 ± 0.575
4.401GluArg: 4.401 ± 0.528
2.954GluSer: 2.954 ± 0.499
3.557GluThr: 3.557 ± 0.475
3.738GluVal: 3.738 ± 0.444
1.266GluTrp: 1.266 ± 0.277
2.291GluTyr: 2.291 ± 0.432
0.0GluXaa: 0.0 ± 0.0
Phe
2.713PheAla: 2.713 ± 0.448
0.603PheCys: 0.603 ± 0.215
3.738PheAsp: 3.738 ± 0.472
2.713PheGlu: 2.713 ± 0.335
0.965PhePhe: 0.965 ± 0.214
3.014PheGly: 3.014 ± 0.361
1.145PheHis: 1.145 ± 0.268
2.17PheIle: 2.17 ± 0.351
2.773PheLys: 2.773 ± 0.306
2.472PheLeu: 2.472 ± 0.375
0.965PheMet: 0.965 ± 0.236
2.231PheAsn: 2.231 ± 0.263
1.145PhePro: 1.145 ± 0.289
1.206PheGln: 1.206 ± 0.246
1.688PheArg: 1.688 ± 0.327
2.351PheSer: 2.351 ± 0.447
2.291PheThr: 2.291 ± 0.469
3.376PheVal: 3.376 ± 0.455
0.663PheTrp: 0.663 ± 0.192
1.206PheTyr: 1.206 ± 0.259
0.0PheXaa: 0.0 ± 0.0
Gly
5.245GlyAla: 5.245 ± 0.719
0.904GlyCys: 0.904 ± 0.23
3.497GlyAsp: 3.497 ± 0.519
5.004GlyGlu: 5.004 ± 0.583
3.256GlyPhe: 3.256 ± 0.504
5.124GlyGly: 5.124 ± 0.721
2.231GlyHis: 2.231 ± 0.374
4.16GlyIle: 4.16 ± 0.393
4.944GlyLys: 4.944 ± 0.495
6.029GlyLeu: 6.029 ± 0.418
1.809GlyMet: 1.809 ± 0.381
3.256GlyAsn: 3.256 ± 0.433
1.809GlyPro: 1.809 ± 0.349
3.014GlyGln: 3.014 ± 0.565
3.617GlyArg: 3.617 ± 0.446
5.124GlySer: 5.124 ± 0.765
5.064GlyThr: 5.064 ± 0.56
6.391GlyVal: 6.391 ± 0.571
1.447GlyTrp: 1.447 ± 0.35
2.773GlyTyr: 2.773 ± 0.456
0.0GlyXaa: 0.0 ± 0.0
His
1.387HisAla: 1.387 ± 0.268
0.301HisCys: 0.301 ± 0.162
1.145HisAsp: 1.145 ± 0.278
1.085HisGlu: 1.085 ± 0.23
1.085HisPhe: 1.085 ± 0.247
1.447HisGly: 1.447 ± 0.347
0.543HisHis: 0.543 ± 0.196
1.567HisIle: 1.567 ± 0.32
1.206HisLys: 1.206 ± 0.279
1.628HisLeu: 1.628 ± 0.359
0.723HisMet: 0.723 ± 0.25
0.904HisAsn: 0.904 ± 0.292
1.085HisPro: 1.085 ± 0.243
0.543HisGln: 0.543 ± 0.192
0.904HisArg: 0.904 ± 0.209
1.025HisSer: 1.025 ± 0.289
1.025HisThr: 1.025 ± 0.218
1.387HisVal: 1.387 ± 0.274
0.663HisTrp: 0.663 ± 0.209
0.603HisTyr: 0.603 ± 0.194
0.0HisXaa: 0.0 ± 0.0
Ile
5.245IleAla: 5.245 ± 0.522
0.663IleCys: 0.663 ± 0.186
3.497IleAsp: 3.497 ± 0.516
3.919IleGlu: 3.919 ± 0.579
1.688IlePhe: 1.688 ± 0.316
4.16IleGly: 4.16 ± 0.449
0.301IleHis: 0.301 ± 0.124
2.653IleIle: 2.653 ± 0.325
3.798IleLys: 3.798 ± 0.418
2.713IleLeu: 2.713 ± 0.415
2.231IleMet: 2.231 ± 0.391
2.713IleAsn: 2.713 ± 0.382
2.532IlePro: 2.532 ± 0.378
1.99IleGln: 1.99 ± 0.383
2.351IleArg: 2.351 ± 0.448
2.231IleSer: 2.231 ± 0.487
3.798IleThr: 3.798 ± 0.414
3.858IleVal: 3.858 ± 0.564
1.085IleTrp: 1.085 ± 0.278
1.99IleTyr: 1.99 ± 0.331
0.0IleXaa: 0.0 ± 0.0
Lys
6.571LysAla: 6.571 ± 0.816
0.723LysCys: 0.723 ± 0.225
3.557LysAsp: 3.557 ± 0.448
3.738LysGlu: 3.738 ± 0.482
2.351LysPhe: 2.351 ± 0.38
3.798LysGly: 3.798 ± 0.474
1.266LysHis: 1.266 ± 0.228
2.653LysIle: 2.653 ± 0.495
3.497LysLys: 3.497 ± 0.733
5.366LysLeu: 5.366 ± 0.561
2.17LysMet: 2.17 ± 0.398
1.929LysAsn: 1.929 ± 0.322
3.858LysPro: 3.858 ± 0.509
2.713LysGln: 2.713 ± 0.428
3.316LysArg: 3.316 ± 0.465
2.653LysSer: 2.653 ± 0.368
2.773LysThr: 2.773 ± 0.388
4.522LysVal: 4.522 ± 0.494
1.085LysTrp: 1.085 ± 0.234
1.387LysTyr: 1.387 ± 0.287
0.0LysXaa: 0.0 ± 0.0
Leu
6.873LeuAla: 6.873 ± 0.589
0.784LeuCys: 0.784 ± 0.258
5.185LeuAsp: 5.185 ± 0.444
5.607LeuGlu: 5.607 ± 0.604
2.713LeuPhe: 2.713 ± 0.435
5.667LeuGly: 5.667 ± 0.456
1.206LeuHis: 1.206 ± 0.273
4.582LeuIle: 4.582 ± 0.525
5.185LeuLys: 5.185 ± 0.615
4.702LeuLeu: 4.702 ± 0.577
2.472LeuMet: 2.472 ± 0.425
4.1LeuAsn: 4.1 ± 0.513
3.858LeuPro: 3.858 ± 0.511
3.436LeuGln: 3.436 ± 0.474
4.823LeuArg: 4.823 ± 0.508
5.426LeuSer: 5.426 ± 0.627
6.27LeuThr: 6.27 ± 0.661
4.763LeuVal: 4.763 ± 0.511
1.085LeuTrp: 1.085 ± 0.237
1.869LeuTyr: 1.869 ± 0.34
0.0LeuXaa: 0.0 ± 0.0
Met
2.532MetAla: 2.532 ± 0.386
0.301MetCys: 0.301 ± 0.126
1.688MetAsp: 1.688 ± 0.321
2.231MetGlu: 2.231 ± 0.428
0.904MetPhe: 0.904 ± 0.36
2.532MetGly: 2.532 ± 0.358
0.181MetHis: 0.181 ± 0.131
1.326MetIle: 1.326 ± 0.293
1.387MetLys: 1.387 ± 0.27
2.412MetLeu: 2.412 ± 0.4
0.362MetMet: 0.362 ± 0.169
1.206MetAsn: 1.206 ± 0.236
1.145MetPro: 1.145 ± 0.273
1.326MetGln: 1.326 ± 0.321
1.266MetArg: 1.266 ± 0.291
1.809MetSer: 1.809 ± 0.346
2.11MetThr: 2.11 ± 0.396
1.809MetVal: 1.809 ± 0.291
0.422MetTrp: 0.422 ± 0.141
0.723MetTyr: 0.723 ± 0.221
0.0MetXaa: 0.0 ± 0.0
Asn
3.738AsnAla: 3.738 ± 0.476
0.723AsnCys: 0.723 ± 0.264
2.05AsnAsp: 2.05 ± 0.386
2.532AsnGlu: 2.532 ± 0.455
1.688AsnPhe: 1.688 ± 0.247
4.522AsnGly: 4.522 ± 0.408
0.784AsnHis: 0.784 ± 0.226
2.231AsnIle: 2.231 ± 0.366
2.11AsnLys: 2.11 ± 0.291
4.522AsnLeu: 4.522 ± 0.552
1.266AsnMet: 1.266 ± 0.244
2.592AsnAsn: 2.592 ± 0.463
2.894AsnPro: 2.894 ± 0.391
1.628AsnGln: 1.628 ± 0.32
1.99AsnArg: 1.99 ± 0.313
2.653AsnSer: 2.653 ± 0.426
3.014AsnThr: 3.014 ± 0.393
3.195AsnVal: 3.195 ± 0.433
0.603AsnTrp: 0.603 ± 0.166
1.326AsnTyr: 1.326 ± 0.29
0.0AsnXaa: 0.0 ± 0.0
Pro
3.195ProAla: 3.195 ± 0.468
0.723ProCys: 0.723 ± 0.197
2.653ProAsp: 2.653 ± 0.432
3.376ProGlu: 3.376 ± 0.369
2.11ProPhe: 2.11 ± 0.313
3.135ProGly: 3.135 ± 0.387
0.784ProHis: 0.784 ± 0.167
2.11ProIle: 2.11 ± 0.355
2.291ProLys: 2.291 ± 0.379
3.497ProLeu: 3.497 ± 0.485
1.025ProMet: 1.025 ± 0.204
1.628ProAsn: 1.628 ± 0.325
1.507ProPro: 1.507 ± 0.308
2.11ProGln: 2.11 ± 0.537
2.472ProArg: 2.472 ± 0.342
2.291ProSer: 2.291 ± 0.351
2.894ProThr: 2.894 ± 0.443
3.738ProVal: 3.738 ± 0.415
0.723ProTrp: 0.723 ± 0.213
1.688ProTyr: 1.688 ± 0.282
0.0ProXaa: 0.0 ± 0.0
Gln
3.316GlnAla: 3.316 ± 0.602
0.603GlnCys: 0.603 ± 0.17
2.11GlnAsp: 2.11 ± 0.375
2.05GlnGlu: 2.05 ± 0.276
1.809GlnPhe: 1.809 ± 0.343
4.22GlnGly: 4.22 ± 0.68
0.603GlnHis: 0.603 ± 0.188
1.748GlnIle: 1.748 ± 0.327
1.447GlnLys: 1.447 ± 0.322
4.883GlnLeu: 4.883 ± 0.623
1.507GlnMet: 1.507 ± 0.309
1.387GlnAsn: 1.387 ± 0.28
2.472GlnPro: 2.472 ± 0.494
5.486GlnGln: 5.486 ± 3.49
2.11GlnArg: 2.11 ± 0.392
1.507GlnSer: 1.507 ± 0.283
1.99GlnThr: 1.99 ± 0.311
2.231GlnVal: 2.231 ± 0.365
1.085GlnTrp: 1.085 ± 0.29
1.145GlnTyr: 1.145 ± 0.304
0.0GlnXaa: 0.0 ± 0.0
Arg
4.22ArgAla: 4.22 ± 0.52
0.844ArgCys: 0.844 ± 0.246
2.834ArgAsp: 2.834 ± 0.384
4.22ArgGlu: 4.22 ± 0.55
2.231ArgPhe: 2.231 ± 0.336
3.256ArgGly: 3.256 ± 0.485
0.844ArgHis: 0.844 ± 0.197
3.256ArgIle: 3.256 ± 0.466
3.557ArgLys: 3.557 ± 0.604
4.16ArgLeu: 4.16 ± 0.527
1.145ArgMet: 1.145 ± 0.218
2.894ArgAsn: 2.894 ± 0.397
1.99ArgPro: 1.99 ± 0.331
2.17ArgGln: 2.17 ± 0.28
2.954ArgArg: 2.954 ± 0.433
2.834ArgSer: 2.834 ± 0.413
3.497ArgThr: 3.497 ± 0.442
3.195ArgVal: 3.195 ± 0.442
0.965ArgTrp: 0.965 ± 0.194
1.628ArgTyr: 1.628 ± 0.368
0.0ArgXaa: 0.0 ± 0.0
Ser
4.763SerAla: 4.763 ± 0.592
0.663SerCys: 0.663 ± 0.22
3.436SerAsp: 3.436 ± 0.45
3.135SerGlu: 3.135 ± 0.466
2.532SerPhe: 2.532 ± 0.385
4.944SerGly: 4.944 ± 0.601
1.206SerHis: 1.206 ± 0.279
2.834SerIle: 2.834 ± 0.449
4.22SerLys: 4.22 ± 0.415
4.642SerLeu: 4.642 ± 0.451
1.085SerMet: 1.085 ± 0.231
3.075SerAsn: 3.075 ± 0.474
2.05SerPro: 2.05 ± 0.343
2.17SerGln: 2.17 ± 0.4
2.291SerArg: 2.291 ± 0.491
2.231SerSer: 2.231 ± 0.399
2.834SerThr: 2.834 ± 0.394
3.557SerVal: 3.557 ± 0.426
0.844SerTrp: 0.844 ± 0.231
2.773SerTyr: 2.773 ± 0.39
0.0SerXaa: 0.0 ± 0.0
Thr
5.185ThrAla: 5.185 ± 0.362
0.844ThrCys: 0.844 ± 0.257
4.1ThrAsp: 4.1 ± 0.511
3.195ThrGlu: 3.195 ± 0.544
3.135ThrPhe: 3.135 ± 0.475
5.366ThrGly: 5.366 ± 0.724
1.266ThrHis: 1.266 ± 0.306
3.316ThrIle: 3.316 ± 0.454
3.014ThrLys: 3.014 ± 0.344
4.883ThrLeu: 4.883 ± 0.567
1.266ThrMet: 1.266 ± 0.343
2.894ThrAsn: 2.894 ± 0.351
3.678ThrPro: 3.678 ± 0.412
2.532ThrGln: 2.532 ± 0.454
3.436ThrArg: 3.436 ± 0.394
4.22ThrSer: 4.22 ± 0.541
4.22ThrThr: 4.22 ± 0.568
3.858ThrVal: 3.858 ± 0.603
0.543ThrTrp: 0.543 ± 0.195
1.748ThrTyr: 1.748 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
5.727ValAla: 5.727 ± 0.529
0.904ValCys: 0.904 ± 0.241
4.341ValAsp: 4.341 ± 0.495
4.702ValGlu: 4.702 ± 0.583
2.351ValPhe: 2.351 ± 0.307
4.883ValGly: 4.883 ± 0.488
1.266ValHis: 1.266 ± 0.291
4.1ValIle: 4.1 ± 0.524
4.16ValLys: 4.16 ± 0.495
3.919ValLeu: 3.919 ± 0.597
1.748ValMet: 1.748 ± 0.274
3.678ValAsn: 3.678 ± 0.479
3.075ValPro: 3.075 ± 0.392
2.834ValGln: 2.834 ± 0.313
4.1ValArg: 4.1 ± 0.442
3.678ValSer: 3.678 ± 0.579
4.28ValThr: 4.28 ± 0.487
4.702ValVal: 4.702 ± 0.587
0.784ValTrp: 0.784 ± 0.233
2.713ValTyr: 2.713 ± 0.417
0.0ValXaa: 0.0 ± 0.0
Trp
0.723TrpAla: 0.723 ± 0.2
0.301TrpCys: 0.301 ± 0.15
1.145TrpAsp: 1.145 ± 0.194
0.482TrpGlu: 0.482 ± 0.163
0.784TrpPhe: 0.784 ± 0.185
1.085TrpGly: 1.085 ± 0.244
0.241TrpHis: 0.241 ± 0.164
0.965TrpIle: 0.965 ± 0.234
0.844TrpLys: 0.844 ± 0.174
1.326TrpLeu: 1.326 ± 0.33
0.181TrpMet: 0.181 ± 0.095
0.723TrpAsn: 0.723 ± 0.154
0.965TrpPro: 0.965 ± 0.187
0.784TrpGln: 0.784 ± 0.296
0.844TrpArg: 0.844 ± 0.214
1.507TrpSer: 1.507 ± 0.268
0.784TrpThr: 0.784 ± 0.248
1.085TrpVal: 1.085 ± 0.273
0.301TrpTrp: 0.301 ± 0.161
1.025TrpTyr: 1.025 ± 0.25
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.412TyrAla: 2.412 ± 0.383
0.301TyrCys: 0.301 ± 0.115
2.05TyrAsp: 2.05 ± 0.325
1.869TyrGlu: 1.869 ± 0.299
1.025TyrPhe: 1.025 ± 0.235
3.135TyrGly: 3.135 ± 0.395
0.543TyrHis: 0.543 ± 0.21
1.748TyrIle: 1.748 ± 0.307
2.412TyrLys: 2.412 ± 0.411
3.256TyrLeu: 3.256 ± 0.434
0.965TyrMet: 0.965 ± 0.253
1.869TyrAsn: 1.869 ± 0.283
1.748TyrPro: 1.748 ± 0.302
0.663TyrGln: 0.663 ± 0.191
2.17TyrArg: 2.17 ± 0.341
1.688TyrSer: 1.688 ± 0.344
2.472TyrThr: 2.472 ± 0.427
2.11TyrVal: 2.11 ± 0.475
0.603TyrTrp: 0.603 ± 0.221
1.145TyrTyr: 1.145 ± 0.269
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (16588 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski