Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; Helicobacter pylori

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1552 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O25055|O25055_HELPY Guanosine pentaphosphate phosphohydrolase (GppA) OS=Helicobacter pylori (strain ATCC 700392 / 26695) OX=85962 GN=HP_0278 PE=4 SV=1
MM1 pKa = 7.38SLLVNDD7 pKa = 4.04EE8 pKa = 5.15CIACDD13 pKa = 3.47ACRR16 pKa = 11.84EE17 pKa = 4.17EE18 pKa = 4.98CPSEE22 pKa = 4.47AIEE25 pKa = 4.64EE26 pKa = 4.25GDD28 pKa = 3.96PIYY31 pKa = 11.08NIDD34 pKa = 3.7PDD36 pKa = 4.06RR37 pKa = 11.84CTEE40 pKa = 4.04CYY42 pKa = 10.42GYY44 pKa = 10.98DD45 pKa = 3.77DD46 pKa = 5.53DD47 pKa = 5.46EE48 pKa = 4.53PRR50 pKa = 11.84CVSVCPVDD58 pKa = 5.97AILPDD63 pKa = 4.21PNNAEE68 pKa = 4.28SKK70 pKa = 11.09EE71 pKa = 3.93EE72 pKa = 3.83LKK74 pKa = 11.1YY75 pKa = 10.7KK76 pKa = 10.74YY77 pKa = 10.1EE78 pKa = 4.08SLKK81 pKa = 10.86EE82 pKa = 3.73QDD84 pKa = 3.08

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P71405|CYSE_HELPY Serine acetyltransferase OS=Helicobacter pylori (strain ATCC 700392 / 26695) OX=85962 GN=cysE PE=3 SV=2
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.37RR14 pKa = 11.84THH16 pKa = 6.01GFLVRR21 pKa = 11.84MKK23 pKa = 9.29TKK25 pKa = 10.53NGRR28 pKa = 11.84KK29 pKa = 9.3VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.86GRR39 pKa = 11.84KK40 pKa = 8.62KK41 pKa = 10.86LSVV44 pKa = 3.15

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1552

0

1552

491826

12

2893

316.9

35.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.845 ± 0.053

1.098 ± 0.027

4.771 ± 0.048

6.895 ± 0.083

5.421 ± 0.057

5.78 ± 0.072

2.121 ± 0.032

7.199 ± 0.054

8.933 ± 0.092

11.217 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.291 ± 0.029

5.815 ± 0.093

3.286 ± 0.04

3.701 ± 0.045

3.446 ± 0.039

6.816 ± 0.055

4.37 ± 0.047

5.609 ± 0.056

0.703 ± 0.017

3.68 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski