Amino acid dipepetide frequency for Nocardia phage NBR1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.063AlaAla: 15.063 ± 1.433
0.633AlaCys: 0.633 ± 0.226
10.136AlaAsp: 10.136 ± 1.016
7.391AlaGlu: 7.391 ± 0.962
3.519AlaPhe: 3.519 ± 0.552
10.91AlaGly: 10.91 ± 0.983
2.112AlaHis: 2.112 ± 0.487
4.434AlaIle: 4.434 ± 0.536
4.294AlaLys: 4.294 ± 0.486
9.291AlaLeu: 9.291 ± 0.845
2.675AlaMet: 2.675 ± 0.457
3.449AlaAsn: 3.449 ± 0.471
5.42AlaPro: 5.42 ± 0.71
4.153AlaGln: 4.153 ± 0.595
8.587AlaArg: 8.587 ± 0.868
5.279AlaSer: 5.279 ± 0.568
7.32AlaThr: 7.32 ± 0.833
7.883AlaVal: 7.883 ± 0.838
1.83AlaTrp: 1.83 ± 0.353
3.308AlaTyr: 3.308 ± 0.616
0.0AlaXaa: 0.0 ± 0.0
Cys
0.845CysAla: 0.845 ± 0.231
0.07CysCys: 0.07 ± 0.079
0.422CysAsp: 0.422 ± 0.184
0.282CysGlu: 0.282 ± 0.142
0.211CysPhe: 0.211 ± 0.117
0.352CysGly: 0.352 ± 0.185
0.211CysHis: 0.211 ± 0.11
0.141CysIle: 0.141 ± 0.09
0.141CysLys: 0.141 ± 0.102
0.493CysLeu: 0.493 ± 0.164
0.141CysMet: 0.141 ± 0.108
0.07CysAsn: 0.07 ± 0.083
0.352CysPro: 0.352 ± 0.143
0.211CysGln: 0.211 ± 0.118
0.352CysArg: 0.352 ± 0.188
0.211CysSer: 0.211 ± 0.113
0.352CysThr: 0.352 ± 0.19
0.352CysVal: 0.352 ± 0.176
0.141CysTrp: 0.141 ± 0.105
0.352CysTyr: 0.352 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
8.517AspAla: 8.517 ± 0.677
0.493AspCys: 0.493 ± 0.178
4.434AspAsp: 4.434 ± 0.682
3.097AspGlu: 3.097 ± 0.434
2.041AspPhe: 2.041 ± 0.361
7.672AspGly: 7.672 ± 0.732
1.267AspHis: 1.267 ± 0.298
3.238AspIle: 3.238 ± 0.557
1.126AspLys: 1.126 ± 0.263
5.772AspLeu: 5.772 ± 0.861
1.83AspMet: 1.83 ± 0.303
1.689AspAsn: 1.689 ± 0.347
3.801AspPro: 3.801 ± 0.619
2.675AspGln: 2.675 ± 0.489
4.786AspArg: 4.786 ± 0.611
2.604AspSer: 2.604 ± 0.341
4.364AspThr: 4.364 ± 0.654
6.687AspVal: 6.687 ± 0.649
1.337AspTrp: 1.337 ± 0.219
1.619AspTyr: 1.619 ± 0.268
0.0AspXaa: 0.0 ± 0.0
Glu
7.531GluAla: 7.531 ± 0.729
0.493GluCys: 0.493 ± 0.167
3.731GluAsp: 3.731 ± 0.619
2.886GluGlu: 2.886 ± 0.603
1.83GluPhe: 1.83 ± 0.392
4.153GluGly: 4.153 ± 0.692
1.126GluHis: 1.126 ± 0.28
1.478GluIle: 1.478 ± 0.3
1.337GluLys: 1.337 ± 0.297
6.265GluLeu: 6.265 ± 0.806
0.985GluMet: 0.985 ± 0.266
0.985GluAsn: 0.985 ± 0.325
2.816GluPro: 2.816 ± 0.572
2.956GluGln: 2.956 ± 0.472
3.238GluArg: 3.238 ± 0.464
2.323GluSer: 2.323 ± 0.411
2.464GluThr: 2.464 ± 0.431
3.238GluVal: 3.238 ± 0.423
1.056GluTrp: 1.056 ± 0.328
1.478GluTyr: 1.478 ± 0.312
0.0GluXaa: 0.0 ± 0.0
Phe
2.745PheAla: 2.745 ± 0.452
0.141PheCys: 0.141 ± 0.093
1.83PheAsp: 1.83 ± 0.437
1.478PheGlu: 1.478 ± 0.372
0.915PhePhe: 0.915 ± 0.269
2.745PheGly: 2.745 ± 0.414
0.774PheHis: 0.774 ± 0.209
0.563PheIle: 0.563 ± 0.233
0.845PheLys: 0.845 ± 0.287
2.323PheLeu: 2.323 ± 0.508
0.282PheMet: 0.282 ± 0.139
0.845PheAsn: 0.845 ± 0.25
1.76PhePro: 1.76 ± 0.457
0.985PheGln: 0.985 ± 0.227
1.408PheArg: 1.408 ± 0.31
2.041PheSer: 2.041 ± 0.396
2.393PheThr: 2.393 ± 0.54
2.816PheVal: 2.816 ± 0.377
0.563PheTrp: 0.563 ± 0.191
0.704PheTyr: 0.704 ± 0.234
0.0PheXaa: 0.0 ± 0.0
Gly
8.447GlyAla: 8.447 ± 0.883
0.493GlyCys: 0.493 ± 0.236
5.349GlyAsp: 5.349 ± 0.733
4.082GlyGlu: 4.082 ± 0.47
3.027GlyPhe: 3.027 ± 0.373
8.024GlyGly: 8.024 ± 1.42
1.689GlyHis: 1.689 ± 0.375
4.294GlyIle: 4.294 ± 0.661
3.801GlyLys: 3.801 ± 0.458
7.109GlyLeu: 7.109 ± 0.933
2.323GlyMet: 2.323 ± 0.361
3.167GlyAsn: 3.167 ± 0.529
6.405GlyPro: 6.405 ± 1.356
4.012GlyGln: 4.012 ± 0.585
6.335GlyArg: 6.335 ± 0.647
5.068GlySer: 5.068 ± 0.745
7.25GlyThr: 7.25 ± 0.903
6.687GlyVal: 6.687 ± 0.726
2.464GlyTrp: 2.464 ± 0.439
3.097GlyTyr: 3.097 ± 0.582
0.0GlyXaa: 0.0 ± 0.0
His
2.252HisAla: 2.252 ± 0.404
0.07HisCys: 0.07 ± 0.069
1.197HisAsp: 1.197 ± 0.331
0.704HisGlu: 0.704 ± 0.224
0.915HisPhe: 0.915 ± 0.237
1.337HisGly: 1.337 ± 0.337
0.563HisHis: 0.563 ± 0.22
0.704HisIle: 0.704 ± 0.229
0.422HisLys: 0.422 ± 0.167
1.549HisLeu: 1.549 ± 0.295
0.282HisMet: 0.282 ± 0.128
0.493HisAsn: 0.493 ± 0.21
1.83HisPro: 1.83 ± 0.33
1.126HisGln: 1.126 ± 0.257
1.549HisArg: 1.549 ± 0.291
0.633HisSer: 0.633 ± 0.225
1.549HisThr: 1.549 ± 0.406
1.337HisVal: 1.337 ± 0.269
0.422HisTrp: 0.422 ± 0.172
0.915HisTyr: 0.915 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
3.308IleAla: 3.308 ± 0.374
0.141IleCys: 0.141 ± 0.096
3.097IleAsp: 3.097 ± 0.474
3.379IleGlu: 3.379 ± 0.488
1.408IlePhe: 1.408 ± 0.401
3.731IleGly: 3.731 ± 0.648
0.563IleHis: 0.563 ± 0.215
1.478IleIle: 1.478 ± 0.369
1.056IleLys: 1.056 ± 0.228
2.393IleLeu: 2.393 ± 0.458
0.493IleMet: 0.493 ± 0.191
1.689IleAsn: 1.689 ± 0.328
2.675IlePro: 2.675 ± 0.466
1.83IleGln: 1.83 ± 0.364
2.675IleArg: 2.675 ± 0.517
2.112IleSer: 2.112 ± 0.453
2.886IleThr: 2.886 ± 0.515
3.238IleVal: 3.238 ± 0.489
0.211IleTrp: 0.211 ± 0.124
1.337IleTyr: 1.337 ± 0.341
0.0IleXaa: 0.0 ± 0.0
Lys
4.927LysAla: 4.927 ± 0.654
0.352LysCys: 0.352 ± 0.182
2.464LysAsp: 2.464 ± 0.368
0.985LysGlu: 0.985 ± 0.249
0.845LysPhe: 0.845 ± 0.221
3.519LysGly: 3.519 ± 0.481
0.633LysHis: 0.633 ± 0.22
1.478LysIle: 1.478 ± 0.321
1.337LysLys: 1.337 ± 0.318
3.308LysLeu: 3.308 ± 0.465
0.774LysMet: 0.774 ± 0.265
0.422LysAsn: 0.422 ± 0.144
1.408LysPro: 1.408 ± 0.344
0.774LysGln: 0.774 ± 0.282
1.549LysArg: 1.549 ± 0.316
1.76LysSer: 1.76 ± 0.393
2.393LysThr: 2.393 ± 0.405
2.604LysVal: 2.604 ± 0.429
0.493LysTrp: 0.493 ± 0.179
0.985LysTyr: 0.985 ± 0.285
0.0LysXaa: 0.0 ± 0.0
Leu
12.459LeuAla: 12.459 ± 1.059
0.633LeuCys: 0.633 ± 0.22
6.335LeuAsp: 6.335 ± 0.7
6.898LeuGlu: 6.898 ± 0.747
2.252LeuPhe: 2.252 ± 0.407
7.391LeuGly: 7.391 ± 0.74
1.83LeuHis: 1.83 ± 0.428
2.534LeuIle: 2.534 ± 0.403
4.012LeuLys: 4.012 ± 0.631
5.631LeuLeu: 5.631 ± 1.006
1.619LeuMet: 1.619 ± 0.339
1.9LeuAsn: 1.9 ± 0.357
3.871LeuPro: 3.871 ± 0.487
2.534LeuGln: 2.534 ± 0.372
4.294LeuArg: 4.294 ± 0.689
3.801LeuSer: 3.801 ± 0.569
5.842LeuThr: 5.842 ± 0.603
6.405LeuVal: 6.405 ± 0.841
0.985LeuTrp: 0.985 ± 0.272
2.182LeuTyr: 2.182 ± 0.451
0.0LeuXaa: 0.0 ± 0.0
Met
3.801MetAla: 3.801 ± 0.424
0.282MetCys: 0.282 ± 0.128
2.041MetAsp: 2.041 ± 0.424
0.985MetGlu: 0.985 ± 0.27
0.422MetPhe: 0.422 ± 0.209
2.041MetGly: 2.041 ± 0.367
0.704MetHis: 0.704 ± 0.215
0.563MetIle: 0.563 ± 0.187
0.563MetLys: 0.563 ± 0.201
2.534MetLeu: 2.534 ± 0.354
0.422MetMet: 0.422 ± 0.209
1.056MetAsn: 1.056 ± 0.213
1.337MetPro: 1.337 ± 0.285
0.282MetGln: 0.282 ± 0.132
1.478MetArg: 1.478 ± 0.341
1.83MetSer: 1.83 ± 0.334
2.464MetThr: 2.464 ± 0.467
1.478MetVal: 1.478 ± 0.295
0.07MetTrp: 0.07 ± 0.07
0.422MetTyr: 0.422 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
3.871AsnAla: 3.871 ± 0.523
0.0AsnCys: 0.0 ± 0.0
0.704AsnAsp: 0.704 ± 0.227
1.337AsnGlu: 1.337 ± 0.247
0.845AsnPhe: 0.845 ± 0.285
3.167AsnGly: 3.167 ± 0.6
0.633AsnHis: 0.633 ± 0.24
1.478AsnIle: 1.478 ± 0.259
0.845AsnLys: 0.845 ± 0.289
1.83AsnLeu: 1.83 ± 0.353
0.845AsnMet: 0.845 ± 0.252
0.774AsnAsn: 0.774 ± 0.254
1.971AsnPro: 1.971 ± 0.448
0.774AsnGln: 0.774 ± 0.206
1.76AsnArg: 1.76 ± 0.417
2.323AsnSer: 2.323 ± 0.423
2.252AsnThr: 2.252 ± 0.353
1.971AsnVal: 1.971 ± 0.409
0.704AsnTrp: 0.704 ± 0.226
0.633AsnTyr: 0.633 ± 0.186
0.0AsnXaa: 0.0 ± 0.0
Pro
6.616ProAla: 6.616 ± 0.776
0.352ProCys: 0.352 ± 0.157
4.434ProAsp: 4.434 ± 0.637
4.223ProGlu: 4.223 ± 0.713
1.689ProPhe: 1.689 ± 0.338
5.772ProGly: 5.772 ± 0.61
0.352ProHis: 0.352 ± 0.148
2.675ProIle: 2.675 ± 0.51
1.337ProLys: 1.337 ± 0.375
4.364ProLeu: 4.364 ± 0.623
1.689ProMet: 1.689 ± 0.389
2.252ProAsn: 2.252 ± 0.365
4.364ProPro: 4.364 ± 0.665
1.83ProGln: 1.83 ± 0.561
2.464ProArg: 2.464 ± 0.382
3.519ProSer: 3.519 ± 0.497
3.66ProThr: 3.66 ± 0.466
5.138ProVal: 5.138 ± 0.646
1.478ProTrp: 1.478 ± 0.294
1.549ProTyr: 1.549 ± 0.322
0.0ProXaa: 0.0 ± 0.0
Gln
3.097GlnAla: 3.097 ± 0.333
0.07GlnCys: 0.07 ± 0.063
1.689GlnAsp: 1.689 ± 0.392
1.478GlnGlu: 1.478 ± 0.284
1.267GlnPhe: 1.267 ± 0.347
4.223GlnGly: 4.223 ± 1.123
0.845GlnHis: 0.845 ± 0.268
2.112GlnIle: 2.112 ± 0.524
0.985GlnLys: 0.985 ± 0.286
4.505GlnLeu: 4.505 ± 0.638
1.267GlnMet: 1.267 ± 0.321
1.126GlnAsn: 1.126 ± 0.304
1.971GlnPro: 1.971 ± 0.416
1.337GlnGln: 1.337 ± 0.257
2.182GlnArg: 2.182 ± 0.427
1.619GlnSer: 1.619 ± 0.444
2.182GlnThr: 2.182 ± 0.392
4.012GlnVal: 4.012 ± 0.616
0.493GlnTrp: 0.493 ± 0.187
0.845GlnTyr: 0.845 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
7.32ArgAla: 7.32 ± 0.713
0.493ArgCys: 0.493 ± 0.241
4.294ArgAsp: 4.294 ± 0.439
3.097ArgGlu: 3.097 ± 0.557
1.549ArgPhe: 1.549 ± 0.227
4.927ArgGly: 4.927 ± 0.75
1.689ArgHis: 1.689 ± 0.32
2.393ArgIle: 2.393 ± 0.403
2.323ArgLys: 2.323 ± 0.4
6.194ArgLeu: 6.194 ± 0.692
1.83ArgMet: 1.83 ± 0.407
2.041ArgAsn: 2.041 ± 0.334
2.816ArgPro: 2.816 ± 0.567
2.534ArgGln: 2.534 ± 0.467
5.279ArgArg: 5.279 ± 0.779
2.534ArgSer: 2.534 ± 0.433
3.167ArgThr: 3.167 ± 0.486
4.082ArgVal: 4.082 ± 0.601
2.182ArgTrp: 2.182 ± 0.359
2.393ArgTyr: 2.393 ± 0.371
0.0ArgXaa: 0.0 ± 0.0
Ser
5.349SerAla: 5.349 ± 0.563
0.211SerCys: 0.211 ± 0.12
3.66SerAsp: 3.66 ± 0.448
1.478SerGlu: 1.478 ± 0.303
0.915SerPhe: 0.915 ± 0.221
6.828SerGly: 6.828 ± 0.752
0.985SerHis: 0.985 ± 0.223
1.619SerIle: 1.619 ± 0.356
1.9SerLys: 1.9 ± 0.323
3.59SerLeu: 3.59 ± 0.694
1.267SerMet: 1.267 ± 0.327
1.619SerAsn: 1.619 ± 0.488
3.097SerPro: 3.097 ± 0.509
1.83SerGln: 1.83 ± 0.371
3.238SerArg: 3.238 ± 0.522
2.956SerSer: 2.956 ± 0.643
3.871SerThr: 3.871 ± 0.534
3.449SerVal: 3.449 ± 0.716
1.267SerTrp: 1.267 ± 0.32
1.056SerTyr: 1.056 ± 0.225
0.0SerXaa: 0.0 ± 0.0
Thr
8.587ThrAla: 8.587 ± 0.916
0.141ThrCys: 0.141 ± 0.092
4.575ThrAsp: 4.575 ± 0.628
2.604ThrGlu: 2.604 ± 0.448
1.478ThrPhe: 1.478 ± 0.352
6.546ThrGly: 6.546 ± 0.97
1.549ThrHis: 1.549 ± 0.337
3.238ThrIle: 3.238 ± 0.463
2.182ThrLys: 2.182 ± 0.351
5.349ThrLeu: 5.349 ± 0.743
2.041ThrMet: 2.041 ± 0.361
1.337ThrAsn: 1.337 ± 0.297
5.49ThrPro: 5.49 ± 0.593
1.619ThrGln: 1.619 ± 0.319
3.167ThrArg: 3.167 ± 0.55
2.886ThrSer: 2.886 ± 0.391
5.209ThrThr: 5.209 ± 0.832
6.476ThrVal: 6.476 ± 0.715
1.549ThrTrp: 1.549 ± 0.371
1.83ThrTyr: 1.83 ± 0.314
0.0ThrXaa: 0.0 ± 0.0
Val
7.743ValAla: 7.743 ± 0.93
0.493ValCys: 0.493 ± 0.277
4.927ValAsp: 4.927 ± 0.576
4.364ValGlu: 4.364 ± 0.522
1.971ValPhe: 1.971 ± 0.331
6.053ValGly: 6.053 ± 0.644
1.408ValHis: 1.408 ± 0.35
2.886ValIle: 2.886 ± 0.469
3.027ValLys: 3.027 ± 0.61
6.757ValLeu: 6.757 ± 0.77
2.464ValMet: 2.464 ± 0.397
2.393ValAsn: 2.393 ± 0.456
5.701ValPro: 5.701 ± 0.707
3.66ValGln: 3.66 ± 0.427
5.349ValArg: 5.349 ± 0.516
4.012ValSer: 4.012 ± 0.43
4.786ValThr: 4.786 ± 0.605
7.32ValVal: 7.32 ± 0.704
1.197ValTrp: 1.197 ± 0.247
2.041ValTyr: 2.041 ± 0.335
0.0ValXaa: 0.0 ± 0.0
Trp
2.182TrpAla: 2.182 ± 0.412
0.0TrpCys: 0.0 ± 0.0
1.619TrpAsp: 1.619 ± 0.348
0.563TrpGlu: 0.563 ± 0.216
0.493TrpPhe: 0.493 ± 0.254
0.915TrpGly: 0.915 ± 0.244
0.563TrpHis: 0.563 ± 0.191
0.985TrpIle: 0.985 ± 0.247
0.845TrpLys: 0.845 ± 0.239
2.112TrpLeu: 2.112 ± 0.309
0.563TrpMet: 0.563 ± 0.192
0.774TrpAsn: 0.774 ± 0.239
0.985TrpPro: 0.985 ± 0.258
0.985TrpGln: 0.985 ± 0.269
1.267TrpArg: 1.267 ± 0.244
1.337TrpSer: 1.337 ± 0.246
1.478TrpThr: 1.478 ± 0.324
0.845TrpVal: 0.845 ± 0.341
0.493TrpTrp: 0.493 ± 0.181
0.633TrpTyr: 0.633 ± 0.207
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.027TyrAla: 3.027 ± 0.526
0.141TyrCys: 0.141 ± 0.089
2.112TyrAsp: 2.112 ± 0.383
1.056TyrGlu: 1.056 ± 0.364
0.563TyrPhe: 0.563 ± 0.186
2.816TyrGly: 2.816 ± 0.34
0.352TyrHis: 0.352 ± 0.177
1.408TyrIle: 1.408 ± 0.271
0.633TyrLys: 0.633 ± 0.218
2.041TyrLeu: 2.041 ± 0.399
0.915TyrMet: 0.915 ± 0.326
0.633TyrAsn: 0.633 ± 0.275
1.689TyrPro: 1.689 ± 0.322
1.197TyrGln: 1.197 ± 0.284
2.252TyrArg: 2.252 ± 0.367
1.337TyrSer: 1.337 ± 0.331
1.971TyrThr: 1.971 ± 0.45
2.534TyrVal: 2.534 ± 0.443
0.704TyrTrp: 0.704 ± 0.206
0.633TyrTyr: 0.633 ± 0.163
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (14208 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski