Beihai hepe-like virus 4
Average proteome isoelectric point is 5.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KJT7|A0A1L3KJT7_9VIRU Replicase OS=Beihai hepe-like virus 4 OX=1922387 PE=4 SV=1
MM1 pKa = 7.87 DD2 pKa = 4.07 FEE4 pKa = 5.68 KK5 pKa = 11.02 YY6 pKa = 9.57 FPKK9 pKa = 10.47 PFIPGQSHH17 pKa = 5.19 RR18 pKa = 11.84 TILQYY23 pKa = 11.23 DD24 pKa = 3.38 QFKK27 pKa = 10.89 AIRR30 pKa = 11.84 SWHH33 pKa = 6.06 ISSVSIASIINLIIGFTVLAALANLEE59 pKa = 3.89 IFIDD63 pKa = 3.87 VEE65 pKa = 4.07 NDD67 pKa = 3.29 GVIAKK72 pKa = 9.83 LDD74 pKa = 3.55 EE75 pKa = 4.67 MKK77 pKa = 10.67 QQMSDD82 pKa = 2.98 NSDD85 pKa = 3.45 RR86 pKa = 11.84 AHH88 pKa = 7.32 LDD90 pKa = 3.1 STDD93 pKa = 3.69 LLKK96 pKa = 10.51 QLKK99 pKa = 10.63 LMVTSLASANNKK111 pKa = 9.46 LDD113 pKa = 4.28 SILVSVNQNRR123 pKa = 11.84 DD124 pKa = 3.38 SNVASAAAAAVIEE137 pKa = 4.34 STILLVHH144 pKa = 5.56 QQLSNMLQMYY154 pKa = 7.95 GANTARR160 pKa = 11.84 DD161 pKa = 4.44 DD162 pKa = 3.77 IFHH165 pKa = 6.79 SKK167 pKa = 9.73 VLSGIDD173 pKa = 3.61 AITVNTGDD181 pKa = 3.58 SAGQATIIANLEE193 pKa = 4.0 EE194 pKa = 4.33 IKK196 pKa = 11.0 ASVAASLAEE205 pKa = 4.0 LTTLNGFQAVIAEE218 pKa = 4.38 EE219 pKa = 4.21 VTDD222 pKa = 3.87 VNVEE226 pKa = 3.94 LVGIGASVATLVVLQQTTGIRR247 pKa = 11.84 IRR249 pKa = 11.84 EE250 pKa = 4.04 HH251 pKa = 6.19 SVTIRR256 pKa = 11.84 DD257 pKa = 3.52 QPILVDD263 pKa = 3.45 VEE265 pKa = 4.42 KK266 pKa = 10.31 FFPVISTRR274 pKa = 11.84 PASGHH279 pKa = 4.92 EE280 pKa = 4.16 WPVKK284 pKa = 9.31 GTVNTIPDD292 pKa = 3.79 FQGGSVTIEE301 pKa = 3.95 GTITTSPAVDD311 pKa = 3.76 SVWTTEE317 pKa = 3.58 PRR319 pKa = 11.84 LPEE322 pKa = 3.93 VLNTSSTPSSGSAWFTNPDD341 pKa = 3.42 LGSTIVEE348 pKa = 4.06 TRR350 pKa = 11.84 ARR352 pKa = 11.84 PVGRR356 pKa = 11.84 NFEE359 pKa = 4.23 VEE361 pKa = 3.81 QGIAEE366 pKa = 4.48 DD367 pKa = 3.61 WTPVDD372 pKa = 3.7 WYY374 pKa = 9.03 TIPVFKK380 pKa = 10.67 SVVRR384 pKa = 11.84 VKK386 pKa = 10.82 PGDD389 pKa = 3.7 DD390 pKa = 3.75 PTWVVAPADD399 pKa = 3.66 NTLWLSNSTAKK410 pKa = 10.84 NEE412 pKa = 4.1 GPISISGPVEE422 pKa = 3.91 TVPLSGSIWHH432 pKa = 6.34 TAAEE436 pKa = 4.38 PVTDD440 pKa = 4.05 TVWDD444 pKa = 4.34 TKK446 pKa = 11.2 ASPTVGAQWMTKK458 pKa = 7.97 ATPVPEE464 pKa = 4.89 TIWSTVPDD472 pKa = 3.76 MGTSIVEE479 pKa = 3.88 TRR481 pKa = 11.84 ARR483 pKa = 11.84 PVGRR487 pKa = 11.84 NFAVEE492 pKa = 3.89 QGIADD497 pKa = 3.9 DD498 pKa = 3.81 WTPVDD503 pKa = 3.96 WYY505 pKa = 8.94 TVPVFKK511 pKa = 10.47 STVKK515 pKa = 10.44 VKK517 pKa = 10.72 PGDD520 pKa = 3.64 DD521 pKa = 3.92 PVWVVSPASNTMWLSNNTASFDD543 pKa = 3.84 GPVVIDD549 pKa = 4.72 GPIEE553 pKa = 3.85 TKK555 pKa = 10.38 PAVGTFWHH563 pKa = 6.32 TVADD567 pKa = 4.16 PKK569 pKa = 10.64 IDD571 pKa = 3.92 AVWSTTPNMGSTIVEE586 pKa = 4.02 TRR588 pKa = 11.84 ARR590 pKa = 11.84 PVGRR594 pKa = 11.84 NFEE597 pKa = 4.38 VEE599 pKa = 4.11 QGTADD604 pKa = 3.64 DD605 pKa = 3.83 WTTVDD610 pKa = 3.61 WYY612 pKa = 9.21 TVPVFKK618 pKa = 10.45 STVRR622 pKa = 11.84 VKK624 pKa = 10.87 PGDD627 pKa = 3.61 DD628 pKa = 3.71 PVWVVSPDD636 pKa = 3.42 AGSVWLSNNTKK647 pKa = 10.6 LSDD650 pKa = 3.85 GPISISGPIEE660 pKa = 3.91 TTPSNGAIWHH670 pKa = 5.98 TNVEE674 pKa = 4.27 NTVVSRR680 pKa = 11.84 IEE682 pKa = 4.14 TVVTVAPEE690 pKa = 4.31 LGSKK694 pKa = 9.51 WEE696 pKa = 4.05 VTQGGEE702 pKa = 4.25 LQVKK706 pKa = 7.94 PAPSTMWPVYY716 pKa = 10.17 PIDD719 pKa = 3.69 GTVWLSNNSEE729 pKa = 4.0 TTIAGVIEE737 pKa = 4.22 TKK739 pKa = 10.15 PVQGSVWHH747 pKa = 5.65 TSIDD751 pKa = 3.68 NEE753 pKa = 3.55 VDD755 pKa = 3.12 ARR757 pKa = 11.84 IQSIVTVTPEE767 pKa = 4.51 LGSKK771 pKa = 9.36 WEE773 pKa = 4.21 VVQSEE778 pKa = 4.54 DD779 pKa = 4.74 FYY781 pKa = 11.58 VIPAAAATWSVYY793 pKa = 9.41 PAAGTVWLSNNTLSQKK809 pKa = 9.65 TSVEE813 pKa = 4.39 GIVYY817 pKa = 7.85 TAPASDD823 pKa = 4.26 AVWVTDD829 pKa = 3.66 PSIPSIINTMPTIPSPLDD847 pKa = 3.31 VQVAGSVVTKK857 pKa = 10.36 PDD859 pKa = 3.36 LTAVGPVVVQQSPNTLWYY877 pKa = 11.19 VNINDD882 pKa = 4.21 TDD884 pKa = 3.96 GPLYY888 pKa = 9.59 TKK890 pKa = 10.34 PDD892 pKa = 3.96 LSPNVAVEE900 pKa = 4.33 VINQVSVVPVAEE912 pKa = 4.51 STWDD916 pKa = 3.64 TIIVDD921 pKa = 3.8 TVGAVATKK929 pKa = 10.24 PDD931 pKa = 3.8 LTPFVSVEE939 pKa = 4.11 VIGTVDD945 pKa = 3.73 TKK947 pKa = 10.32 PTLTEE952 pKa = 3.97 TVEE955 pKa = 4.15 VEE957 pKa = 4.24 PSSSAFWLTQAVPADD972 pKa = 3.31 GWDD975 pKa = 3.38 WSVKK979 pKa = 9.91 PAVQTVWQSSPVTVVNTKK997 pKa = 9.47 PQFSEE1002 pKa = 4.04 AVEE1005 pKa = 4.2 VEE1007 pKa = 4.29 PSSSAFWLTQAIPADD1022 pKa = 3.48 GWDD1025 pKa = 3.47 WSVKK1029 pKa = 9.91 PAVQTVWQSSPVTVVNTKK1047 pKa = 9.63 PQFSDD1052 pKa = 3.66 PVPVTDD1058 pKa = 3.78 SDD1060 pKa = 4.31 VVAKK1064 pKa = 10.35 LDD1066 pKa = 4.28 SIEE1069 pKa = 4.3 CTVTDD1074 pKa = 3.35 ITLADD1079 pKa = 3.57 NVSIPVRR1086 pKa = 11.84 QDD1088 pKa = 3.6 YY1089 pKa = 10.82 INPWYY1094 pKa = 10.64 VEE1096 pKa = 3.96 QEE1098 pKa = 4.15 PNDD1101 pKa = 3.56 WWTMVQVPGHH1111 pKa = 4.77 VWAVKK1116 pKa = 7.37 EE1117 pKa = 4.67 TPAKK1121 pKa = 10.59 APTVRR1126 pKa = 11.84 ANKK1129 pKa = 10.03 YY1130 pKa = 10.35 DD1131 pKa = 3.5 LL1132 pKa = 4.29
Molecular weight: 122.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.489
IPC2_protein 4.304
IPC_protein 4.304
Toseland 4.113
ProMoST 4.431
Dawson 4.279
Bjellqvist 4.418
Wikipedia 4.164
Rodwell 4.139
Grimsley 4.024
Solomon 4.266
Lehninger 4.228
Nozaki 4.38
DTASelect 4.584
Thurlkill 4.139
EMBOSS 4.177
Sillero 4.418
Patrickios 4.024
IPC_peptide 4.279
IPC2_peptide 4.406
IPC2.peptide.svr19 4.362
Protein with the highest isoelectric point:
>tr|A0A1L3KJU7|A0A1L3KJU7_9VIRU Uncharacterized protein OS=Beihai hepe-like virus 4 OX=1922387 PE=4 SV=1
MM1 pKa = 7.75 LILYY5 pKa = 9.74 LYY7 pKa = 10.89 LLLLLPYY14 pKa = 10.41 GSSNYY19 pKa = 8.68 EE20 pKa = 3.87 KK21 pKa = 10.47 PILTDD26 pKa = 3.29 TPDD29 pKa = 4.46 CIIHH33 pKa = 5.97 YY34 pKa = 7.35 PTTKK38 pKa = 9.95 GRR40 pKa = 11.84 KK41 pKa = 8.94 CITSSRR47 pKa = 11.84 LYY49 pKa = 11.0 LLGFFPLLFLTTATFMAHH67 pKa = 5.47 PTALFLLIPHH77 pKa = 6.93 FALFSFDD84 pKa = 3.23 VNLRR88 pKa = 11.84 HH89 pKa = 6.66 LLLLAGDD96 pKa = 4.2 IEE98 pKa = 5.23 SNPGPGVGPLHH109 pKa = 5.62 VPKK112 pKa = 10.38 YY113 pKa = 9.61 SSEE116 pKa = 4.22 IIQKK120 pKa = 10.22 KK121 pKa = 8.13 YY122 pKa = 10.76 AEE124 pKa = 4.34 SFIDD128 pKa = 4.04 SIADD132 pKa = 3.33 NRR134 pKa = 11.84 INDD137 pKa = 3.78 AVPIDD142 pKa = 4.35 FNLHH146 pKa = 6.45 DD147 pKa = 4.81 DD148 pKa = 4.4 QVGTLLNDD156 pKa = 3.17 YY157 pKa = 10.87 GLLVKK162 pKa = 10.48 PNTRR166 pKa = 11.84 APYY169 pKa = 9.31 EE170 pKa = 3.81 NHH172 pKa = 6.45 GMCKK176 pKa = 9.18 TLTAYY181 pKa = 10.24 AAEE184 pKa = 4.3 VVNRR188 pKa = 11.84 KK189 pKa = 7.42 TDD191 pKa = 3.37 NLDD194 pKa = 4.19 FIEE197 pKa = 4.37 VAPDD201 pKa = 3.72 FANLKK206 pKa = 10.05 SNRR209 pKa = 11.84 HH210 pKa = 4.9 YY211 pKa = 10.11 CTLLSSDD218 pKa = 3.16 ASDD221 pKa = 3.08 RR222 pKa = 11.84 ARR224 pKa = 11.84 ANRR227 pKa = 11.84 VLSLRR232 pKa = 11.84 PNSNRR237 pKa = 11.84 AEE239 pKa = 4.16 EE240 pKa = 4.2 LRR242 pKa = 11.84 NVLNGTPSDD251 pKa = 3.8 LMCTHH256 pKa = 7.07 GFQNCRR262 pKa = 11.84 HH263 pKa = 5.3 QSNIGVAKK271 pKa = 9.12 MVHH274 pKa = 6.76 DD275 pKa = 5.33 IDD277 pKa = 5.46 PEE279 pKa = 4.07 MLPGIMSRR287 pKa = 11.84 HH288 pKa = 4.6 GMNFLFVTMFLPPEE302 pKa = 4.87 LYY304 pKa = 10.54 AKK306 pKa = 9.8 TPIDD310 pKa = 4.18 EE311 pKa = 4.79 GDD313 pKa = 3.14 LGYY316 pKa = 11.1 RR317 pKa = 11.84 LAYY320 pKa = 10.5 DD321 pKa = 3.28 SDD323 pKa = 3.82 KK324 pKa = 11.54 AIMTFYY330 pKa = 10.66 DD331 pKa = 3.66 GEE333 pKa = 3.75 IGYY336 pKa = 6.82 THH338 pKa = 7.41 NYY340 pKa = 7.47 TNWRR344 pKa = 11.84 KK345 pKa = 8.46 WFQPAFTDD353 pKa = 3.42 GTTIVKK359 pKa = 10.03 IEE361 pKa = 3.77 VSKK364 pKa = 10.99 RR365 pKa = 11.84 LGPVVVLEE373 pKa = 4.11 CSRR376 pKa = 11.84 TFGMLTAVRR385 pKa = 11.84 AVGGSEE391 pKa = 4.37 DD392 pKa = 4.07 NVPIPIWVNHH402 pKa = 5.55 FTDD405 pKa = 4.01 MLDD408 pKa = 3.64 KK409 pKa = 10.66 ANHH412 pKa = 5.9 MFFSMLKK419 pKa = 8.3 GSCYY423 pKa = 9.99 RR424 pKa = 11.84 RR425 pKa = 11.84 WVDD428 pKa = 3.2 KK429 pKa = 10.51 TLEE432 pKa = 4.12 TLEE435 pKa = 4.51 KK436 pKa = 9.57 VTIPRR441 pKa = 11.84 KK442 pKa = 8.67 MLDD445 pKa = 2.97 RR446 pKa = 11.84 VFLFNLCRR454 pKa = 11.84 EE455 pKa = 4.09 DD456 pKa = 3.45 TAIKK460 pKa = 9.88 RR461 pKa = 11.84 QSSHH465 pKa = 7.05 AYY467 pKa = 8.69 IRR469 pKa = 11.84 AINSKK474 pKa = 9.94 ISIGATTIQRR484 pKa = 11.84 EE485 pKa = 4.55 TNVPTRR491 pKa = 11.84 HH492 pKa = 5.67 IQPLLTQLFVMGCVLRR508 pKa = 11.84 ARR510 pKa = 11.84 DD511 pKa = 3.74 TKK513 pKa = 10.9 FSGVALNALKK523 pKa = 10.54 GSHH526 pKa = 7.39 DD527 pKa = 3.52 GGHH530 pKa = 5.8 WYY532 pKa = 10.03 EE533 pKa = 4.55 YY534 pKa = 10.11 ISDD537 pKa = 5.4 AITVLTGQPPAKK549 pKa = 10.0 SRR551 pKa = 11.84 NPHH554 pKa = 5.49 RR555 pKa = 11.84 AGLVVEE561 pKa = 4.36 YY562 pKa = 10.19 LCDD565 pKa = 3.5 VTVPTTLTTHH575 pKa = 6.16 VALEE579 pKa = 4.34 VPVKK583 pKa = 10.73 DD584 pKa = 3.92 NIMSVRR590 pKa = 11.84 RR591 pKa = 11.84 EE592 pKa = 4.07 GEE594 pKa = 3.83 HH595 pKa = 6.28 VKK597 pKa = 10.6 FVDD600 pKa = 5.51 DD601 pKa = 4.55 DD602 pKa = 4.44 NKK604 pKa = 11.08 CLGHH608 pKa = 6.25 SMSVLIHH615 pKa = 6.21 GNSLHH620 pKa = 6.39 TDD622 pKa = 3.52 HH623 pKa = 7.02 YY624 pKa = 10.65 HH625 pKa = 7.38 DD626 pKa = 4.62 LVKK629 pKa = 10.91 GDD631 pKa = 3.47 VAIAEE636 pKa = 4.53 SIQPGISAFLHH647 pKa = 5.47 VRR649 pKa = 11.84 GQHH652 pKa = 5.01 VEE654 pKa = 4.02 PVVNLAEE661 pKa = 4.27 YY662 pKa = 9.3 CAHH665 pKa = 5.98 SVRR668 pKa = 11.84 YY669 pKa = 9.75 YY670 pKa = 9.79 ITDD673 pKa = 4.17 LAIKK677 pKa = 10.29 DD678 pKa = 3.92 VLCIPARR685 pKa = 11.84 EE686 pKa = 4.01 YY687 pKa = 10.42 FANISVGEE695 pKa = 4.22 PIFSQGYY702 pKa = 8.86 HH703 pKa = 5.64 VPSCLTFVEE712 pKa = 4.67 KK713 pKa = 10.53 FRR715 pKa = 11.84 SYY717 pKa = 11.06 LRR719 pKa = 11.84 NYY721 pKa = 9.89 CNGEE725 pKa = 3.44 IAIYY729 pKa = 9.15 KK730 pKa = 7.95 QQSATTITSSKK741 pKa = 9.75 LDD743 pKa = 3.63 NYY745 pKa = 10.39 KK746 pKa = 9.28 ATPEE750 pKa = 4.27 VVHH753 pKa = 6.3 SKK755 pKa = 10.22 YY756 pKa = 11.13 SFWKK760 pKa = 8.46 TSSASEE766 pKa = 4.13 LLDD769 pKa = 3.51 SVAANVNIYY778 pKa = 10.0 KK779 pKa = 10.56 ARR781 pKa = 11.84 LHH783 pKa = 7.1 LIALQNALPTEE794 pKa = 4.28 DD795 pKa = 3.92 VEE797 pKa = 5.06 KK798 pKa = 9.65 KK799 pKa = 8.79 TKK801 pKa = 10.28 FSAVFNCSKK810 pKa = 10.34 RR811 pKa = 11.84 RR812 pKa = 11.84 FIMKK816 pKa = 9.94 QPSLAILCASPGNDD830 pKa = 3.21 LQYY833 pKa = 11.46 LFDD836 pKa = 3.78 ISNVNEE842 pKa = 4.08 VCVTPGIDD850 pKa = 3.24 STRR853 pKa = 11.84 VLKK856 pKa = 10.8 LCEE859 pKa = 4.25 SKK861 pKa = 10.82 GVKK864 pKa = 9.39 VLQNFNAACQNCVDD878 pKa = 4.8 KK879 pKa = 11.33 IEE881 pKa = 4.55 SNYY884 pKa = 10.26 VYY886 pKa = 11.11 ADD888 pKa = 3.51 LGGEE892 pKa = 4.32 NDD894 pKa = 3.6 TQTCVSTVFACLNSLKK910 pKa = 10.12 TKK912 pKa = 9.99 TFVLKK917 pKa = 8.99 WQKK920 pKa = 10.71 GMQAIAEE927 pKa = 4.34 RR928 pKa = 11.84 TPGTAQVFDD937 pKa = 4.05 LCILNRR943 pKa = 11.84 FTIYY947 pKa = 10.56 NIPEE951 pKa = 4.06 VEE953 pKa = 4.24 TTEE956 pKa = 4.57 LWITNSKK963 pKa = 10.12 LAVSISGPSIDD974 pKa = 5.3 LGPVSNFFRR983 pKa = 11.84 LHH985 pKa = 6.52 LDD987 pKa = 3.02 AAKK990 pKa = 10.51 YY991 pKa = 5.33 WTQHH995 pKa = 3.33 EE996 pKa = 4.64 WIRR999 pKa = 11.84 FEE1001 pKa = 4.88 GGANMSAEE1009 pKa = 4.64 EE1010 pKa = 4.43 IEE1012 pKa = 4.36 LACFKK1017 pKa = 11.21 NPDD1020 pKa = 3.02 WRR1022 pKa = 11.84 NWEE1025 pKa = 3.87 PSAPPAHH1032 pKa = 7.01 LMPPAPGPQAVYY1044 pKa = 11.01 DD1045 pKa = 4.14 DD1046 pKa = 3.86 VWRR1049 pKa = 11.84 PGLPALWEE1057 pKa = 4.2 DD1058 pKa = 3.72 VVARR1062 pKa = 11.84 GVSTTSVGVGNDD1074 pKa = 3.51 DD1075 pKa = 3.3 LTKK1078 pKa = 9.97 YY1079 pKa = 10.39 LEE1081 pKa = 4.39 HH1082 pKa = 6.56 FRR1084 pKa = 11.84 SPTKK1088 pKa = 10.3 VLKK1091 pKa = 10.67 VDD1093 pKa = 4.84 ASTCTDD1099 pKa = 3.19 NVKK1102 pKa = 10.36 EE1103 pKa = 4.04 MPEE1106 pKa = 3.95 VGVQTEE1112 pKa = 4.25 LVVPKK1117 pKa = 8.54 TTAEE1121 pKa = 4.17 FSTQCDD1127 pKa = 3.42 LDD1129 pKa = 4.07 VSVGTSIKK1137 pKa = 9.92 PKK1139 pKa = 9.9 PFVPYY1144 pKa = 9.58 EE1145 pKa = 3.69 MSIIIEE1151 pKa = 4.4 DD1152 pKa = 3.82 TDD1154 pKa = 3.78 TEE1156 pKa = 4.36 PEE1158 pKa = 3.7 SDD1160 pKa = 3.78 YY1161 pKa = 11.39 SDD1163 pKa = 4.29 EE1164 pKa = 4.32 YY1165 pKa = 10.47 EE1166 pKa = 4.03 QVRR1169 pKa = 11.84 DD1170 pKa = 5.03 FITKK1174 pKa = 7.19 VTSVTTSASIDD1185 pKa = 3.61 EE1186 pKa = 4.97 ALNEE1190 pKa = 4.4 ADD1192 pKa = 4.22 DD1193 pKa = 4.09 TCSLFDD1199 pKa = 4.81 EE1200 pKa = 5.22 EE1201 pKa = 6.45 DD1202 pKa = 3.94 DD1203 pKa = 4.78 FDD1205 pKa = 5.6 DD1206 pKa = 4.54 RR1207 pKa = 11.84 LNEE1210 pKa = 4.55 IISKK1214 pKa = 10.15 PPVRR1218 pKa = 11.84 PPRR1221 pKa = 11.84 RR1222 pKa = 11.84 PKK1224 pKa = 10.65 APPSPPPTVTDD1235 pKa = 3.8 IARR1238 pKa = 11.84 QMAPKK1243 pKa = 9.97 GVVPAVVPSVVEE1255 pKa = 4.2 TKK1257 pKa = 10.33 PEE1259 pKa = 4.03 PVTKK1263 pKa = 10.54 VQTVKK1268 pKa = 10.54 PKK1270 pKa = 10.28 KK1271 pKa = 9.46 PNNARR1276 pKa = 11.84 EE1277 pKa = 4.21 WKK1279 pKa = 8.11 QTHH1282 pKa = 4.24 QAIIITEE1289 pKa = 4.12 SKK1291 pKa = 10.48 LISNAHH1297 pKa = 6.74 DD1298 pKa = 3.83 VYY1300 pKa = 11.43 QLDD1303 pKa = 4.0 FNDD1306 pKa = 4.75 AGLLDD1311 pKa = 4.22 MLRR1314 pKa = 11.84 PIAKK1318 pKa = 9.67 KK1319 pKa = 10.17 SSNDD1323 pKa = 3.09 IVFVAPVITNEE1334 pKa = 3.97 IKK1336 pKa = 11.08 YY1337 pKa = 8.76 MAAKK1341 pKa = 10.25 ASNEE1345 pKa = 3.82 LKK1347 pKa = 10.58 RR1348 pKa = 11.84 EE1349 pKa = 4.08 CFYY1352 pKa = 11.4 LEE1354 pKa = 3.63 QSKK1357 pKa = 8.67 FVCIACQSDD1366 pKa = 2.75 AGIRR1370 pKa = 11.84 TKK1372 pKa = 11.17 DD1373 pKa = 3.38 PVHH1376 pKa = 6.55 PKK1378 pKa = 10.33 VGTTRR1383 pKa = 11.84 FSVDD1387 pKa = 2.86 SKK1389 pKa = 9.81 QCRR1392 pKa = 11.84 IINEE1396 pKa = 4.11 AFQKK1400 pKa = 10.37 EE1401 pKa = 4.67 LGVMTLPFTAALKK1414 pKa = 9.71 QKK1416 pKa = 10.14 YY1417 pKa = 8.2 VAIQPFEE1424 pKa = 4.39 GEE1426 pKa = 3.88 VEE1428 pKa = 4.41 YY1429 pKa = 11.31 VHH1431 pKa = 6.81 GVAGSGKK1438 pKa = 7.3 TRR1440 pKa = 11.84 QAVTQLEE1447 pKa = 4.34 LDD1449 pKa = 3.26 EE1450 pKa = 4.35 WTAVSPFKK1458 pKa = 10.93 RR1459 pKa = 11.84 NLDD1462 pKa = 3.65 EE1463 pKa = 4.15 FTKK1466 pKa = 10.6 GGQYY1470 pKa = 9.57 PANSWTFHH1478 pKa = 5.42 QALLKK1483 pKa = 10.14 YY1484 pKa = 10.63 DD1485 pKa = 3.67 GMHH1488 pKa = 6.62 NLLVDD1493 pKa = 4.17 EE1494 pKa = 5.29 APCFNPYY1501 pKa = 9.9 VLASLVNTAMSNGKK1515 pKa = 9.4 RR1516 pKa = 11.84 VMLLGDD1522 pKa = 4.05 PKK1524 pKa = 10.62 QIRR1527 pKa = 11.84 GVDD1530 pKa = 3.05 ASNYY1534 pKa = 9.61 YY1535 pKa = 10.22 KK1536 pKa = 11.01 GLDD1539 pKa = 3.37 STDD1542 pKa = 4.16 FYY1544 pKa = 11.37 HH1545 pKa = 5.98 GTLYY1549 pKa = 9.26 GTHH1552 pKa = 6.25 SFSVAPEE1559 pKa = 3.47 AAKK1562 pKa = 9.75 WLRR1565 pKa = 11.84 RR1566 pKa = 11.84 LKK1568 pKa = 10.63 IPITTDD1574 pKa = 2.33 GCARR1578 pKa = 11.84 MIMHH1582 pKa = 7.1 KK1583 pKa = 10.45 PGDD1586 pKa = 3.71 TSSITGEE1593 pKa = 4.05 AFCVTKK1599 pKa = 10.77 QMAAAKK1605 pKa = 9.09 NRR1607 pKa = 11.84 HH1608 pKa = 5.72 GSSTWACLTSTQGTRR1623 pKa = 11.84 PEE1625 pKa = 3.95 VMHH1628 pKa = 6.95 VYY1630 pKa = 10.38 FEE1632 pKa = 4.34 EE1633 pKa = 4.13 QGLPLLKK1640 pKa = 10.62 AQGWALLYY1648 pKa = 10.51 VALTRR1653 pKa = 11.84 ATKK1656 pKa = 9.61 EE1657 pKa = 3.71 VHH1659 pKa = 6.42 VYY1661 pKa = 10.46 ASPAFLSKK1669 pKa = 10.94 LGFQQLKK1676 pKa = 9.94 KK1677 pKa = 8.91 HH1678 pKa = 4.99 THH1680 pKa = 5.14 NMPKK1684 pKa = 9.75 QVGNIDD1690 pKa = 3.66 HH1691 pKa = 6.46 NTPAVADD1698 pKa = 3.97 TNDD1701 pKa = 3.7 DD1702 pKa = 3.95 LKK1704 pKa = 11.15 EE1705 pKa = 4.04 LEE1707 pKa = 4.33 PTAEE1711 pKa = 3.91 EE1712 pKa = 3.94 AAEE1715 pKa = 3.96 YY1716 pKa = 10.47 AALTEE1721 pKa = 3.81 KK1722 pKa = 10.81 SRR1724 pKa = 11.84 FIRR1727 pKa = 11.84 RR1728 pKa = 11.84 DD1729 pKa = 2.97 ATEE1732 pKa = 3.94 ARR1734 pKa = 11.84 FQFTDD1739 pKa = 3.37 VEE1741 pKa = 4.12 IGKK1744 pKa = 9.27 IEE1746 pKa = 3.87 EE1747 pKa = 4.2 DD1748 pKa = 3.19 FRR1750 pKa = 11.84 LGHH1753 pKa = 5.44 SQSGKK1758 pKa = 9.81 IKK1760 pKa = 9.66 RR1761 pKa = 11.84 TLLSRR1766 pKa = 11.84 GRR1768 pKa = 11.84 KK1769 pKa = 8.97 SCWPLPSLRR1778 pKa = 11.84 KK1779 pKa = 9.74 YY1780 pKa = 10.84 PNGEE1784 pKa = 4.2 KK1785 pKa = 10.19 PSLPCMEE1792 pKa = 4.55 LTKK1795 pKa = 10.23 STWVLALNDD1804 pKa = 3.99 RR1805 pKa = 11.84 TKK1807 pKa = 11.07 KK1808 pKa = 8.87 PAKK1811 pKa = 8.08 PTSTSQTITIHH1822 pKa = 6.05 KK1823 pKa = 7.2 EE1824 pKa = 3.61 KK1825 pKa = 10.14 PSRR1828 pKa = 11.84 TFYY1831 pKa = 10.79 RR1832 pKa = 11.84 SSLKK1836 pKa = 9.16 NTLSQLHH1843 pKa = 4.96 QTSSQKK1849 pKa = 10.82 LPTLNSMITCQEE1861 pKa = 3.73 QGPSCRR1867 pKa = 11.84 EE1868 pKa = 3.81 LTPTSLTKK1876 pKa = 10.36 SYY1878 pKa = 10.76 PSSTKK1883 pKa = 9.85 QAPTLMTYY1891 pKa = 10.56 SPALRR1896 pKa = 11.84 SEE1898 pKa = 3.93 VLRR1901 pKa = 11.84 RR1902 pKa = 11.84 IEE1904 pKa = 4.11 RR1905 pKa = 11.84 SCVSQTIKK1913 pKa = 10.48 PRR1915 pKa = 11.84 LGMQKK1920 pKa = 9.52 FSTYY1924 pKa = 9.94 RR1925 pKa = 11.84 RR1926 pKa = 11.84 FNAHH1930 pKa = 6.46 LYY1932 pKa = 8.87 GRR1934 pKa = 11.84 KK1935 pKa = 8.21 STASSDD1941 pKa = 4.05 NILDD1945 pKa = 3.85 TLLKK1949 pKa = 10.77 RR1950 pKa = 11.84 FDD1952 pKa = 3.87 NDD1954 pKa = 2.99 GMSFTQSARR1963 pKa = 11.84 EE1964 pKa = 4.02 VGTLLLNSFMEE1975 pKa = 4.26 IVEE1978 pKa = 4.2 QVTPITLEE1986 pKa = 3.8 QVMLKK1991 pKa = 10.31 LAEE1994 pKa = 3.98 SFQRR1998 pKa = 11.84 VKK2000 pKa = 10.59 IKK2002 pKa = 10.94 NDD2004 pKa = 3.26 VIHH2007 pKa = 7.31 AMEE2010 pKa = 5.48 FDD2012 pKa = 3.75 PDD2014 pKa = 3.48 IYY2016 pKa = 11.39 NSFIKK2021 pKa = 10.4 ISSFNKK2027 pKa = 9.69 AQTKK2031 pKa = 10.28 VKK2033 pKa = 9.36 MAEE2036 pKa = 3.61 EE2037 pKa = 5.08 SYY2039 pKa = 10.59 LQTEE2043 pKa = 4.35 GGVLKK2048 pKa = 10.73 AGQPISAQPKK2058 pKa = 9.7 FMTHH2062 pKa = 6.59 LASAFNSAIEE2072 pKa = 3.95 EE2073 pKa = 4.23 NIRR2076 pKa = 11.84 NDD2078 pKa = 3.6 LKK2080 pKa = 10.77 EE2081 pKa = 4.0 GFYY2084 pKa = 10.57 LGYY2087 pKa = 10.99 GMNEE2091 pKa = 3.92 RR2092 pKa = 11.84 EE2093 pKa = 4.2 LDD2095 pKa = 3.61 LLVRR2099 pKa = 11.84 SRR2101 pKa = 11.84 VPTEE2105 pKa = 3.57 YY2106 pKa = 11.0 SFMSVEE2112 pKa = 3.99 SDD2114 pKa = 2.77 ITQMDD2119 pKa = 4.22 SVRR2122 pKa = 11.84 GQATNQNFMAKK2133 pKa = 10.06 VYY2135 pKa = 10.59 DD2136 pKa = 4.39 LYY2138 pKa = 11.58 GMPAICHH2145 pKa = 5.62 KK2146 pKa = 10.9 VADD2149 pKa = 4.46 ALNMKK2154 pKa = 8.83 WVADD2158 pKa = 4.01 ANIVSLLVKK2167 pKa = 10.49 GRR2169 pKa = 11.84 FHH2171 pKa = 7.77 SGRR2174 pKa = 11.84 FDD2176 pKa = 3.5 TLSSNTIVNLALSNYY2191 pKa = 8.67 AFKK2194 pKa = 10.49 IEE2196 pKa = 4.11 RR2197 pKa = 11.84 PHH2199 pKa = 7.0 LVLAMGDD2206 pKa = 3.57 DD2207 pKa = 4.5 FAAIAEE2213 pKa = 4.72 RR2214 pKa = 11.84 ITLVNDD2220 pKa = 3.68 FEE2222 pKa = 4.29 FLKK2225 pKa = 11.31 VNFTDD2230 pKa = 4.21 VPEE2233 pKa = 4.26 FTNNLIGDD2241 pKa = 4.47 RR2242 pKa = 11.84 LYY2244 pKa = 10.61 PSIVKK2249 pKa = 10.3 KK2250 pKa = 10.62 AGKK2253 pKa = 9.47 LLNRR2257 pKa = 11.84 EE2258 pKa = 3.72 FRR2260 pKa = 11.84 DD2261 pKa = 3.86 AQDD2264 pKa = 3.32 LEE2266 pKa = 4.33 AYY2268 pKa = 10.16 RR2269 pKa = 11.84 FAVKK2273 pKa = 10.28 DD2274 pKa = 3.39 WIKK2277 pKa = 10.96 NYY2279 pKa = 10.25 RR2280 pKa = 11.84 DD2281 pKa = 3.74 TLSMHH2286 pKa = 6.71 VICAVNSYY2294 pKa = 10.68 KK2295 pKa = 10.93 YY2296 pKa = 10.62 GIDD2299 pKa = 3.62 VEE2301 pKa = 4.53 QVQALWSFLRR2311 pKa = 11.84 NFADD2315 pKa = 3.55 AKK2317 pKa = 10.21 IVSSFDD2323 pKa = 3.38 DD2324 pKa = 3.44 SSLIKK2329 pKa = 10.53 YY2330 pKa = 10.29 SEE2332 pKa = 4.28 VPSLLIEE2339 pKa = 4.16 NQEE2342 pKa = 4.06 FKK2344 pKa = 10.74 TRR2346 pKa = 3.49
Molecular weight: 263.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.429
IPC2_protein 6.532
IPC_protein 6.532
Toseland 6.649
ProMoST 7.029
Dawson 6.985
Bjellqvist 7.059
Wikipedia 6.971
Rodwell 6.985
Grimsley 6.766
Solomon 6.985
Lehninger 6.985
Nozaki 7.351
DTASelect 7.366
Thurlkill 7.41
EMBOSS 7.38
Sillero 7.424
Patrickios 4.228
IPC_peptide 7.0
IPC2_peptide 6.985
IPC2.peptide.svr19 6.836
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
4062
179
2346
1015.5
112.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.509 ± 0.382
1.305 ± 0.472
5.933 ± 0.187
5.441 ± 0.219
3.816 ± 0.542
5.022 ± 0.369
2.265 ± 0.428
5.785 ± 0.339
5.441 ± 0.718
7.656 ± 1.11
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.068 ± 0.367
4.751 ± 0.118
5.982 ± 0.674
3.397 ± 0.119
4.037 ± 0.678
7.681 ± 0.502
8.05 ± 0.842
9.232 ± 1.667
1.748 ± 0.79
2.88 ± 0.566
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here