Candidatus Aquirickettsiella gammari
Average proteome isoelectric point is 7.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1375 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A370CG80|A0A370CG80_9COXI DM13 domain-containing protein OS=Candidatus Aquirickettsiella gammari OX=2016198 GN=CFE62_005880 PE=4 SV=1
MM1 pKa = 7.72 CLLCGYY7 pKa = 10.55 VYY9 pKa = 10.68 DD10 pKa = 4.78 EE11 pKa = 4.26 EE12 pKa = 5.33 KK13 pKa = 10.62 GWEE16 pKa = 4.07 EE17 pKa = 4.53 DD18 pKa = 3.35 GVAAGTRR25 pKa = 11.84 WEE27 pKa = 4.57 DD28 pKa = 3.55 VPLTWRR34 pKa = 11.84 CPEE37 pKa = 4.22 CGAMKK42 pKa = 10.32 EE43 pKa = 3.97 DD44 pKa = 3.89 FEE46 pKa = 4.48 MVEE49 pKa = 4.0 II50 pKa = 4.86
Molecular weight: 5.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.835
IPC2_protein 4.05
IPC_protein 3.884
Toseland 3.732
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.732
Rodwell 3.732
Grimsley 3.643
Solomon 3.821
Lehninger 3.77
Nozaki 3.973
DTASelect 4.062
Thurlkill 3.77
EMBOSS 3.745
Sillero 3.999
Patrickios 0.693
IPC_peptide 3.821
IPC2_peptide 3.973
IPC2.peptide.svr19 3.891
Protein with the highest isoelectric point:
>tr|A0A370CIR6|A0A370CIR6_9COXI DNA helicase OS=Candidatus Aquirickettsiella gammari OX=2016198 GN=CFE62_002800 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.32 QPSNLKK11 pKa = 10.19 RR12 pKa = 11.84 KK13 pKa = 7.57 RR14 pKa = 11.84 THH16 pKa = 5.9 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.2 KK26 pKa = 10.6 GRR28 pKa = 11.84 LILKK32 pKa = 9.67 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.51 GRR39 pKa = 11.84 LRR41 pKa = 11.84 LTAA44 pKa = 4.67
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1375
0
1375
444153
27
2853
323.0
36.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.293 ± 0.062
1.16 ± 0.023
4.425 ± 0.045
5.449 ± 0.076
4.374 ± 0.049
5.965 ± 0.079
2.333 ± 0.034
7.576 ± 0.049
6.293 ± 0.07
12.177 ± 0.094
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.991 ± 0.026
4.577 ± 0.053
4.135 ± 0.042
4.826 ± 0.055
4.763 ± 0.056
6.462 ± 0.05
5.101 ± 0.063
5.679 ± 0.06
1.118 ± 0.023
3.304 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here